##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522090_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	33483537
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.599135808143565	39.0	38.0	40.0	33.0	40.0
2	37.24555264278084	39.0	36.0	40.0	33.0	40.0
3	37.178619003123835	39.0	36.0	40.0	33.0	40.0
4	37.1527229336614	39.0	36.0	40.0	33.0	40.0
5	37.146624623318615	39.0	36.0	40.0	33.0	40.0
6	37.141857205826255	39.0	36.0	40.0	33.0	40.0
7	37.06921646300389	39.0	36.0	40.0	32.0	40.0
8	37.01422860434368	39.0	36.0	40.0	31.0	40.0
9	36.85951953044865	39.0	36.0	40.0	31.0	40.0
10	36.84182653702325	39.0	36.0	40.0	31.0	40.0
11	36.962921240966864	39.0	36.0	40.0	31.0	40.0
12	37.027427060647746	39.0	36.0	40.0	32.0	40.0
13	36.9995484945333	39.0	36.0	40.0	31.0	40.0
14	37.0446635909462	39.0	36.0	40.0	32.0	40.0
15	37.04857655868315	39.0	36.0	40.0	32.0	40.0
16	37.202384323973895	39.0	36.0	40.0	33.0	40.0
17	37.01044877666299	39.0	36.0	40.0	32.0	40.0
18	36.967973007152736	39.0	36.0	40.0	31.0	40.0
19	36.91854773287541	39.0	36.0	40.0	31.0	40.0
20	36.89415906091403	39.0	36.0	40.0	31.0	40.0
21	36.95210347700125	39.0	36.0	40.0	32.0	40.0
22	36.91512055611091	39.0	36.0	40.0	31.0	40.0
23	36.8520272216164	38.0	36.0	40.0	31.0	40.0
24	36.87970610751188	38.0	36.0	40.0	31.0	40.0
25	36.81985278317521	38.0	36.0	40.0	31.0	40.0
26	36.75860357882741	39.0	36.0	40.0	31.0	40.0
27	36.65376313141589	38.0	36.0	40.0	31.0	40.0
28	36.59964360993285	38.0	35.0	40.0	31.0	40.0
29	36.562539286097525	38.0	35.0	40.0	31.0	40.0
30	36.49199267687879	38.0	35.0	40.0	31.0	40.0
31	36.45723395350975	38.0	35.0	40.0	31.0	40.0
32	36.327745900918416	38.0	35.0	40.0	30.0	40.0
33	36.24926754303167	38.0	35.0	40.0	30.0	40.0
34	36.1581058476588	38.0	35.0	40.0	30.0	40.0
35	36.29198157888756	38.0	35.0	40.0	30.0	40.0
36	36.285152282448536	38.0	35.0	40.0	30.0	40.0
37	36.09210069414112	38.0	35.0	40.0	30.0	40.0
38	36.078445804575544	38.0	35.0	40.0	30.0	40.0
39	36.02449352946196	38.0	35.0	40.0	29.0	40.0
40	35.808123168110946	38.0	35.0	40.0	29.0	40.0
41	35.99322831396217	38.0	35.0	40.0	30.0	40.0
42	35.946046142018986	38.0	35.0	40.0	29.0	40.0
43	35.985168860744906	38.0	35.0	40.0	30.0	40.0
44	35.9973019576755	38.0	35.0	40.0	30.0	40.0
45	35.90682806299705	38.0	35.0	40.0	30.0	40.0
46	35.98513158869686	38.0	35.0	40.0	30.0	40.0
47	35.8430776294631	38.0	35.0	40.0	30.0	40.0
48	35.72112357783468	38.0	35.0	40.0	29.0	40.0
49	35.630320954443974	38.0	35.0	40.0	29.0	40.0
50	35.450317688958606	38.0	35.0	40.0	29.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1	1	-0.22117491778654852
1	2	-0.11181644054358486
1	3	-0.3613994576204931
1	4	-0.2160328755584615
1	5	-0.20600997496062234
1	6	-1.0544818426947415
1	7	-0.22914363291661743
1	8	0.01521350573860758
1	9	-0.12593260723652833
1	10	0.03596373825181587
1	11	-0.064677499792829
1	12	-0.17269489593375198
1	13	-0.057879746429236434
1	14	-0.044635404105228815
1	15	-0.08550870655494691
1	16	-0.041923791042286496
1	17	-0.10250986237556958
1	18	-0.04088262343936577
1	19	0.007610129062527449
1	20	-0.05142883865555348
1	21	-0.07173246805086109
1	22	-0.01640981463978619
1	23	-0.024903153926345567
1	24	-0.04748467308859716
1	25	0.055344700559871285
1	26	-0.04961987495574505
1	27	-0.03276617887851785
1	28	0.021025108988581565
1	29	-0.03330461362694592
1	30	-0.03005724260617626
1	31	-0.00883641650461442
1	32	-0.023201751076619814
1	33	0.019018227887087846
1	34	0.004520957671822146
1	35	0.06367704375239924
1	36	0.22492100712828744
1	37	0.25841320494894404
1	38	0.2052129912828633
1	39	0.20025832946556932
1	40	0.2845312534104494
1	41	0.19104057519172102
1	42	0.15414666218800477
1	43	0.12495317799842809
1	44	0.13203256002520192
1	45	0.08583678972149755
1	46	0.05674581260726086
1	47	0.0211386221369807
1	48	0.041850150735854186
1	49	0.010750423970591783
1	50	0.032775796832851256
2	1	-0.03829820722676658
2	2	-0.021618957130286276
2	3	-0.29352342957346167
2	4	-0.10671743608183704
2	5	-0.21731334023606053
2	6	-0.17473673431747727
2	7	0.039844146554997906
2	8	-0.14270304838256465
2	9	-0.03400427174875631
2	10	-0.10193545936228077
2	11	-0.17191348164015352
2	12	0.011216712631011205
2	13	-0.02608581766640583
2	14	-0.13397162086848624
2	15	-0.0937152782914552
2	16	-0.022090545872657685
2	17	0.09235885159824164
2	18	-0.2778826765224949
2	19	-0.16617019907480568
2	20	-0.1103935416153945
2	21	-0.1043767287158417
2	22	-0.26387364921868794
2	23	-0.19017724567932248
2	24	-0.05112691266037217
2	25	-0.1381708161058839
2	26	-0.016515280174033364
2	27	-0.09497417847536838
2	28	-0.19346062851995782
2	29	-0.12108883625491984
2	30	-0.11974112321669139
2	31	-0.10448263921195178
2	32	-0.08265768324031342
2	33	-0.13310860621334797
2	34	-0.1162827553296708
2	35	-0.05440068647202878
2	36	0.058220796866649493
2	37	-0.0019614425311971218
2	38	-0.11223250372349014
2	39	0.009129094414106476
2	40	-0.0598217967295227
2	41	-0.010916378081148537
2	42	-0.2316416775339789
2	43	-0.20094400943253987
2	44	-0.22944617801338296
2	45	-0.16690640978664106
2	46	-0.23803667248659366
2	47	-0.2093711502112825
2	48	-0.22350001618728754
2	49	-0.2051250937181308
2	50	-0.3289286238912652
3	1	-0.13599219030592025
3	2	-0.16218069078509956
3	3	-0.2561624751566143
3	4	-0.060706006935603796
3	5	-0.4591204152459767
3	6	-0.5262306132162351
3	7	-0.05040634394194399
3	8	-0.11215229773795699
3	9	0.03134232600832121
3	10	-0.14404078963672617
3	11	-0.2867676332363942
3	12	-0.19425183056965523
3	13	-0.019001480803297
3	14	-0.0027436826603448594
3	15	-0.07776598883275199
3	16	0.0335295559940576
3	17	0.09793613871975992
3	18	-0.10511858956440534
3	19	-0.06885348523592683
3	20	0.04442940518466543
3	21	-0.12453642919944485
3	22	-0.06161018022358178
3	23	-0.25942885699615204
3	24	-0.15757213392411984
3	25	-0.1151359273890904
3	26	-0.05945265802564137
3	27	-0.12574238045870345
3	28	-0.07338630417863357
3	29	-0.14702694445742992
3	30	-0.13781277676478254
3	31	-0.16727720881545594
3	32	-0.1306804748018564
3	33	-0.1475869599873647
3	34	-0.10639006220613112
3	35	-0.08876426868245346
3	36	-0.07243874311026843
3	37	-0.3140916534157725
3	38	-0.32264472040256464
3	39	-0.09296222323254
3	40	-0.47178298748322334
3	41	-0.32869590864933684
3	42	-0.16319361321765768
3	43	-0.1504951717395926
3	44	-0.2436505703474765
3	45	-0.16525099061025372
3	46	-0.2900682101900216
3	47	-0.22176153418408262
3	48	-0.2560368237683619
3	49	-0.1679455428534098
3	50	-0.21800601027266708
4	1	0.006153025544385571
4	2	-0.05899899609454451
4	3	-0.055097220258751634
4	4	0.04916578867651111
4	5	8.347427503991867E-4
4	6	-0.05123893346837605
4	7	-0.11372088034038796
4	8	0.07901711531706468
4	9	0.02228795567309305
4	10	0.0871264322835259
4	11	-0.04293364684071577
4	12	-0.06906113021191373
4	13	-0.14993737809935936
4	14	-0.010153562067799271
4	15	-0.014631393514171975
4	16	-0.11342948176519485
4	17	-0.07521095224137042
4	18	-0.1459572324472873
4	19	-0.11827342531834262
4	20	-0.35631432091172854
4	21	-0.24518478574980662
4	22	-0.1864963292820363
4	23	-0.19238782447023084
4	24	-0.23438420519188696
4	25	-0.20464971065949555
4	26	-0.20116954490823957
4	27	-0.1446442131413619
4	28	-0.2504844283122978
4	29	-0.1427522494528759
4	30	-0.19302979457202696
4	31	-0.1380137546933824
4	32	-0.2716986628669815
4	33	-0.42057207259249907
4	34	-0.224285513661016
4	35	-0.2529434351280955
4	36	-0.17315565310924086
4	37	-0.4266805511608851
4	38	-0.2259038070296171
4	39	-0.14604964094768746
4	40	-0.8479473952012668
4	41	-0.4473559525006081
4	42	-0.2769289097252994
4	43	-0.5828830598952095
4	44	-0.37476191796070424
4	45	-0.4232182386704224
4	46	-0.2963677444345123
4	47	-0.43355870501731175
4	48	-0.34323087801424634
4	49	-0.38898507382128145
4	50	-0.38847929284288796
5	1	0.027880782186464614
5	2	-0.3195694348647251
5	3	-0.12266733662387708
5	4	0.02270088090218536
5	5	0.11182089918249005
5	6	-0.0022280080810617164
5	7	0.13749162640997525
5	8	-0.07249088683990834
5	9	-0.55860767131702
5	10	-0.0179649892789584
5	11	-0.3202603941751292
5	12	-0.14557036507349608
5	13	-0.06088897857927833
5	14	-0.21988907704098182
5	15	0.00842484772983454
5	16	-0.11328036582317225
5	17	0.020953334663424528
5	18	-0.10254800734153946
5	19	-0.1360672333033861
5	20	-0.040034090344946094
5	21	-0.001704259645201489
5	22	-0.1312879710734549
5	23	-0.018744751780040758
5	24	0.00920974984854439
5	25	0.008652633688036815
5	26	-0.012144181159385425
5	27	-0.02290365661398397
5	28	-0.015584073909835183
5	29	-0.092770524916304
5	30	-0.12936336778033564
5	31	-0.038607038219126366
5	32	-0.034349102124735964
5	33	-0.1204650872106896
5	34	-0.18028987070672997
5	35	-0.07828407847775054
5	36	-0.2362603565862642
5	37	0.0325163140872462
5	38	-0.25618039966845174
5	39	-0.08242414655094166
5	40	-0.023703737631542765
5	41	-0.10828665668085335
5	42	-0.11116508751712928
5	43	0.08532708418078983
5	44	-0.14378555045801988
5	45	-0.07876625695053008
5	46	-0.0561318710960137
5	47	-0.20117167333390995
5	48	0.03743738372570249
5	49	-0.1703103124686578
5	50	-0.044859061017120894
6	1	-0.1692362342875029
6	2	-0.1411658074304114
6	3	-0.32246859053812216
6	4	-0.10754465189029361
6	5	-0.17177549042159512
6	6	-0.5062531322221915
6	7	-0.09321848614020212
6	8	-0.12333353095213795
6	9	-0.5114342804390262
6	10	-0.06594479035449297
6	11	-0.1485921989384238
6	12	-0.23060892080319917
6	13	-0.14241599953040662
6	14	-0.27368290979964627
6	15	-0.12159193367197219
6	16	-0.17310406183197813
6	17	-0.11733753165930949
6	18	-0.31133541001445764
6	19	-0.11223049735821178
6	20	-0.09455873428837691
6	21	-0.13718408104156055
6	22	-0.2752910106776696
6	23	-0.18275983645755645
6	24	-0.16366190447456574
6	25	-0.10808194272532035
6	26	-0.3111089302928107
6	27	-0.1546548324523016
6	28	-0.13040272098258754
6	29	-0.13387838228244675
6	30	-0.2103840104849155
6	31	-0.12757001897458764
6	32	-0.08623367699104989
6	33	-0.1484461361402012
6	34	-0.11874717522790235
6	35	-0.22887117347519847
6	36	-0.089100530468194
6	37	-0.03553718410530138
6	38	-0.18876535396605476
6	39	-0.24001192044598696
6	40	-0.072639589000417
6	41	-0.3932888074900305
6	42	-0.017822090000251478
6	43	-0.2902632012968809
6	44	-0.20921403696998198
6	45	-0.2687404775772464
6	46	-0.2228461551135723
6	47	-0.28442032102645953
6	48	-0.2614481408492537
6	49	-0.3678997436544904
6	50	-0.38592020780745173
7	1	-0.08435950857792562
7	2	-0.02038150901005764
7	3	-0.0512406924459512
7	4	-0.005113397946828968
7	5	-0.07508258010884816
7	6	-0.1684971563597344
7	7	-0.014925250248559507
7	8	-0.10095974704345423
7	9	-0.11335639025043065
7	10	-0.3234698876484572
7	11	-0.023624332455860042
7	12	-0.21952148777692315
7	13	-0.11682330746931058
7	14	-0.04191373288242062
7	15	-0.03208028555109621
7	16	-0.18452010665502883
7	17	-0.0881058779365631
7	18	0.00446946083196309
7	19	-0.19094089491885313
7	20	-0.12157821643580746
7	21	-0.031822162533622134
7	22	-0.16744620829811652
7	23	-0.11128811469741606
7	24	-0.19246902978082403
7	25	-0.05023685429653568
7	26	-0.053569201384419785
7	27	-0.18464818435453623
7	28	-0.20337989245092558
7	29	-0.20182821880726465
7	30	-0.1555162322387531
7	31	-0.1652188791937732
7	32	-0.15965953824024126
7	33	-0.11678714695670322
7	34	-0.1252809551742189
7	35	-0.12025517451407097
7	36	-0.058131993611610255
7	37	-0.042608564205103505
7	38	-0.305836027955273
7	39	-0.3108876801712128
7	40	-0.31038217932594847
7	41	-0.2603407550503931
7	42	-0.29766397295345115
7	43	-0.25797306210375126
7	44	-0.20073336672761855
7	45	-0.23004702823801182
7	46	-0.32536570814635013
7	47	-0.24058985057578042
7	48	-0.27313109704038396
7	49	-0.2188803635443506
7	50	-0.22280333380994222
8	1	-0.09109191496398239
8	2	-0.0049374946559339605
8	3	-0.01270497175008245
8	4	-0.029875188153063448
8	5	-0.02714581040193309
8	6	-0.09622781950946546
8	7	-0.15614487471439276
8	8	0.07927403680543677
8	9	-0.3102220978763057
8	10	-0.3038528373157021
8	11	0.26480896303863233
8	12	-0.2094888789533016
8	13	0.16751780676655414
8	14	-0.1554458261999372
8	15	-0.053796376211664665
8	16	-0.08998504717089872
8	17	-0.09288505939591118
8	18	0.04087595832044144
8	19	-0.1615852054718161
8	20	0.09072362293429848
8	21	-0.1950706347976805
8	22	-0.1861515317974778
8	23	-0.07472199351848019
8	24	-0.2775156101580194
8	25	-0.10137045077857465
8	26	-0.045157497766467714
8	27	-0.14862801540277815
8	28	-0.24254941665235918
8	29	-0.11546661478162434
8	30	-0.1481890713425642
8	31	-0.15993641039933237
8	32	-0.15316763714863413
8	33	-0.2745508909681149
8	34	-0.14865732488410544
8	35	-0.07933927416526387
8	36	-0.18723269289443323
8	37	-0.10006221259385484
8	38	-0.1545045160197276
8	39	-0.1580312187111943
8	40	-0.22512505362079338
8	41	-0.09494747806642323
8	42	-0.25056064950196344
8	43	-0.10035190965498231
8	44	-0.13978622001340568
8	45	-0.2357214992112162
8	46	-0.24570965452095095
8	47	-0.20229185716206644
8	48	-0.24985265338413143
8	49	-0.21829855143505483
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116	20	-0.2666312193164586
116	21	-0.21879425352756954
116	22	-0.18969512252915166
116	23	-0.18883637714086632
116	24	-0.34748463611993685
116	25	-0.2225854635911162
116	26	-0.1907636997626483
116	27	-0.18248786762978142
116	28	-0.20728853068383302
116	29	-0.24281178430313588
116	30	-0.3550521368103432
116	31	-0.19019902481339557
116	32	-0.306275440258716
116	33	-0.1805916226338553
116	34	-0.23479123188909767
116	35	-0.21786538326302463
116	36	-0.1231828161261106
116	37	-0.056176190252642755
116	38	-0.10307840314094108
116	39	-0.17530185995136094
116	40	-0.12079548075479352
116	41	-0.09391477118126801
116	42	-0.18396872317240565
116	43	-0.1386681394720739
116	44	-0.18654481994143168
116	45	-0.18281047238069448
116	46	-0.13867430222445165
116	47	-0.1194620683904759
116	48	-0.2459645058888711
116	49	-0.11034723441142091
116	50	-0.06366569029255942
117	1	-0.2022941749714633
117	2	-0.277425426920189
117	3	-0.19555569197396494
117	4	-0.18780542161132985
117	5	-0.142582318432801
117	6	-0.05948654226543937
117	7	-0.14324819894913787
117	8	-0.30330996720451253
117	9	-0.0046260103353361615
117	10	-0.35584261837128395
117	11	-0.2927600919168114
117	12	-0.44032720599259534
117	13	-0.3213480716558408
117	14	-0.359083598846496
117	15	-0.31102226667317723
117	16	-0.30880520126617483
117	17	-0.16121424906576465
117	18	-0.15779025473420205
117	19	-0.2607075986803693
117	20	-0.2194955158876013
117	21	-0.1936360334833367
117	22	-0.2004210989560562
117	23	-0.12361074489168544
117	24	-0.21978534443166353
117	25	-0.2275965782748557
117	26	-0.2589808868414565
117	27	-0.3368720615395304
117	28	-0.267534158669541
117	29	-0.33866718176100363
117	30	-0.4203780833383348
117	31	-0.44715571862228387
117	32	-0.4286178877610993
117	33	-0.4563021087263195
117	34	-0.45807528935797137
117	35	-0.45065459490964344
117	36	-0.32465736591402106
117	37	-0.12846566403820248
117	38	-0.1139954403625083
117	39	-0.09872722491267893
117	40	0.06743199407633682
117	41	-0.07640321760502644
117	42	-0.09782407695603013
117	43	-0.12318677919437704
117	44	-0.20767889123237637
117	45	-0.2721771130210513
117	46	-0.30036694428474675
117	47	-0.21283302115195823
117	48	-0.2388419023026742
117	49	-0.26802712303162934
117	50	-0.19051644246361832
118	1	-0.3585973536309197
118	2	-0.32429449865632876
118	3	-0.3099839286576014
118	4	-0.3932444190399167
118	5	-0.514689771557947
118	6	-0.587898741700208
118	7	-0.12614451146986738
118	8	-0.18626875809378163
118	9	-0.14845143061811683
118	10	-0.13593485533953498
118	11	-0.04242920743451606
118	12	-0.06381413493495813
118	13	-0.11786386826572226
118	14	-0.3500256974531908
118	15	-0.22081738606193824
118	16	-0.34209797204277237
118	17	-0.2100311754069537
118	18	-0.5186436107305639
118	19	-0.17417603451377772
118	20	-0.2624264298267107
118	21	-0.16802572944087757
118	22	-0.20865438585752827
118	23	-0.2178897370559696
118	24	-0.33175371037791024
118	25	-0.38036180520347784
118	26	-0.3233770406728098
118	27	-0.438029571705556
118	28	-0.3670995863324862
118	29	-0.4315363612613581
118	30	-0.4951327812901667
118	31	-0.3923602439307743
118	32	-0.424119811324168
118	33	-0.4425052971202206
118	34	-0.39326509283565514
118	35	-0.4169583091046789
118	36	-0.2768843537718624
118	37	-0.5066445839873239
118	38	-0.5839325381651932
118	39	-0.3918940725475011
118	40	-0.2931178268313701
118	41	-0.30242479044251525
118	42	-0.22746202627437384
118	43	-0.42387923936972527
118	44	-0.8331779836380306
118	45	-0.6934202159470502
118	46	-0.5361476016783726
118	47	-0.6515667418794919
118	48	-0.48955497191469277
118	49	-0.41038216021544827
118	50	-0.5937264572699377
119	1	-0.3520396526339269
119	2	-0.4333969628372074
119	3	-0.41331765595410275
119	4	-0.41770223604065393
119	5	-0.33371910896907053
119	6	-0.650663888507097
119	7	-0.5519562146196364
119	8	-0.25462598102469514
119	9	-0.22522125141030358
119	10	-0.20568941001977947
119	11	-0.027860363310701075
119	12	-0.17106732973736882
119	13	-0.24147186441788193
119	14	-0.2981777556444456
119	15	-0.3570211405231021
119	16	-0.37166467678210324
119	17	-0.24163137625767916
119	18	-0.2976617119675069
119	19	-0.2563928621296654
119	20	-0.36793869903811327
119	21	-0.4805458515209011
119	22	-0.3460650539258552
119	23	-0.40534192398624214
119	24	-0.48976045960139913
119	25	-0.4459059548856743
119	26	-0.5235995437080092
119	27	-0.4201443056852554
119	28	-0.44327296411864836
119	29	-0.5604186509408322
119	30	-0.6014440829969558
119	31	-0.5683482671371607
119	32	-0.5710883117366521
119	33	-0.6194941786650503
119	34	-0.6058000766475331
119	35	-0.5337218022061307
119	36	-0.3663023542544863
119	37	-0.4766288669716161
119	38	-0.4146201581898339
119	39	-0.4020130556901833
119	40	-0.241716770837769
119	41	-0.3537567188232984
119	42	-0.4607474065361572
119	43	-0.6079175278903364
119	44	-0.6229703587824531
119	45	-0.5636741483143055
119	46	-0.6281461157401509
119	47	-0.5983967990918728
119	48	-0.5690996483953512
119	49	-0.6597941878091618
119	50	-0.848365444068726
120	1	-0.3437303837614749
120	2	-0.4552367625868925
120	3	-0.4222745934955796
120	4	-0.4436882296626621
120	5	-0.31579158732404267
120	6	-0.19948982377469804
120	7	-0.30843918156166694
120	8	-0.19228385793601177
120	9	-0.28605532314541904
120	10	-0.14640253496680344
120	11	-0.029526825751133856
120	12	-0.4978944993341514
120	13	-0.2013132668122637
120	14	-0.3801014439749153
120	15	-0.40471676033082815
120	16	-0.4248343859474204
120	17	-0.3548423107898344
120	18	-0.3397366599252294
120	19	-0.4839660831362167
120	20	-0.25104889455462853
120	21	-0.22408602234846597
120	22	-0.3524209402098606
120	23	-0.3616707296358257
120	24	-0.4821022946484632
120	25	-0.3628149542739436
120	26	-0.450723703104579
120	27	-0.4280414979641378
120	28	-0.49411659553020115
120	29	-0.49869356258642483
120	30	-0.5626877404739119
120	31	-0.49726502523343186
120	32	-0.4563635059935791
120	33	-0.5466990369355926
120	34	-0.5273737566318744
120	35	-0.4870297359348399
120	36	-0.23695594194273895
120	37	-0.12486556824341477
120	38	-0.2562914819228794
120	39	-0.14117973947407592
120	40	-0.3145263736732318
120	41	-0.04637473148249427
120	42	-0.18290997817175736
120	43	-0.30921640672374195
120	44	-0.35880920563378993
120	45	-0.300451491432554
120	46	-0.417422916419369
120	47	-0.38848644378309416
120	48	-0.32560119638296925
120	49	-0.40669220383986016
120	50	-0.45159918346991645
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	25472.0
3	1161.0
4	1018.0
5	1188.0
6	2047.0
7	2968.0
8	3677.0
9	4775.0
10	6429.0
11	8601.0
12	11594.0
13	15519.0
14	19584.0
15	23666.0
16	27177.0
17	31562.0
18	36097.0
19	41503.0
20	47139.0
21	52985.0
22	63720.0
23	79988.0
24	105544.0
25	144702.0
26	204408.0
27	284899.0
28	383427.0
29	498980.0
30	641378.0
31	810464.0
32	1016482.0
33	1291802.0
34	1696805.0
35	2300363.0
36	3153467.0
37	4567045.0
38	5318290.0
39	1.053192E7
40	25691.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	13.625334934031095	29.156744635816313	30.44238236518063	26.775538064971965
2	22.402158095583687	31.78649308721009	25.736657684556967	20.074691132649257
3	22.397886005763727	29.356314976898346	26.698136860400197	21.54766215693773
4	23.081393701029853	27.427493696379805	26.778082016843086	22.71303058574726
5	24.400892892528052	28.598967904734796	27.90725782643572	19.092881376301435
6	24.45922006387796	27.92730051189037	24.54864012723626	23.064839296995416
7	23.219007597674047	27.777770311422	28.171244274462403	20.831977816441555
8	23.05054271894872	28.471057284061718	26.804459158541107	21.67394083844846
9	22.526324375342284	27.19431143118614	27.951537394909398	22.32782679856217
10	22.140459653351435	28.985097960230426	26.247982702663702	22.626459683754437
11	22.884694051288548	27.80768053267491	26.71650847400022	22.591116942036322
12	21.957835577525756	27.560812945179595	27.44626710135193	23.03508437594272
13	22.700776802641847	27.557665129582936	27.284545835166696	22.45701223260852
14	22.831423518880865	27.895124683978977	27.34967744740907	21.923774349731083
15	21.865527527751922	27.977265364767167	27.930460273656276	22.226746833824635
16	21.829871199091063	27.79351237594762	28.0784643510033	22.298152073958015
17	22.27026374185021	27.548030544084995	27.45768763915234	22.724018074912454
18	21.766386269168635	28.132314695427784	27.552208716779237	22.549090318624344
19	22.654580966162566	27.760042196259015	27.48693186146971	22.09844497610871
20	22.346841773756694	27.431870195209523	27.516723903796226	22.704564127237557
21	22.012895960217314	28.27156496650336	27.4313812211259	22.28415785215343
22	22.761469082887306	27.387377959551618	27.42450982974651	22.426643127814568
23	22.71725324437622	27.451584144633333	27.63879929640361	22.19236331458683
24	22.035444777882088	28.10696258296842	27.435981794268834	22.421610844880654
25	22.197561268602914	27.931917449260595	27.379968530378473	22.490552751758017
26	22.423568957072522	27.49128911951035	28.047464061028105	22.037677862389028
27	22.968781056846392	27.367946475837474	27.447045752539108	22.21622671477703
28	22.367991974781983	27.409841766025938	27.330730753696546	22.89143550549553
29	22.466999146615148	27.446640744096147	27.363877230157968	22.722482879130737
30	22.954916833104814	27.36363500850281	27.37931518008195	22.30213297831043
31	22.33366086656989	27.93337083683497	27.413940368237384	22.319027928357755
32	23.084917514844317	27.38881170955124	27.2958250317161	22.230445743888342
33	22.37579704716613	27.954014903562502	27.281658865082825	22.388529184188542
34	22.327403140813047	27.468141122628737	27.8783943178398	22.326061418718414
35	22.475521326458363	27.427089896401817	27.322787999708954	22.774600777430866
36	22.959480994324608	27.353551549908218	27.330623480339323	22.35634397542785
37	22.926076892058386	27.451098892858894	27.22767328770917	22.39515092737355
38	22.509883370419214	28.062935269236995	27.29925972510835	22.127921635235445
39	22.426030192014377	27.879371129877512	27.289067263798483	22.40553141430963
40	22.876333835601045	27.394986909039165	27.387549372250987	22.341129883108803
41	22.507689474980257	28.055535354262407	27.247810831075558	22.18896433968178
42	23.018556402973765	27.40386127416582	27.253321131546482	22.324261191313933
43	22.42014511167481	27.406834594379497	27.340265577009653	22.832754716936037
44	22.96991032456262	27.522504921373375	27.26435170968792	22.243233044376094
45	22.810023459148262	27.432316407604635	27.385306056988835	22.372354076258265
46	22.387407746091466	27.35908696852104	27.89624236897355	22.35726291641394
47	22.53774938456736	27.351158314106105	27.800399333320662	22.31069296800587
48	22.3582533850161	27.31636017188161	27.254421795708982	23.07096464739331
49	22.375150638099417	27.81473682876588	27.307323182231023	22.502789350903676
50	23.027918131998607	27.365615493213056	27.310017438278223	22.29644893651011
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2027.0
1	1211.5
2	396.0
3	343.0
4	290.0
5	351.0
6	412.0
7	811.0
8	1210.0
9	1928.5
10	2647.0
11	4531.0
12	6415.0
13	11250.0
14	16085.0
15	28224.5
16	40364.0
17	66162.5
18	91961.0
19	135349.0
20	178737.0
21	257117.5
22	335498.0
23	432315.0
24	529132.0
25	591033.5
26	652935.0
27	742041.0
28	831147.0
29	923764.0
30	1016381.0
31	1152902.5
32	1289424.0
33	1425432.0
34	1561440.0
35	1698634.5
36	1835829.0
37	1917825.0
38	1999821.0
39	2075766.5
40	2151712.0
41	2208551.5
42	2265391.0
43	2268918.5
44	2272446.0
45	2238933.5
46	2205421.0
47	2158621.0
48	2111821.0
49	2060056.0
50	2008291.0
51	1955071.0
52	1901851.0
53	1818478.5
54	1735106.0
55	1633506.0
56	1531906.0
57	1524072.0
58	1516238.0
59	1314182.5
60	1112127.0
61	961319.5
62	810512.0
63	685985.0
64	561458.0
65	463929.0
66	366400.0
67	302137.5
68	237875.0
69	189252.5
70	140630.0
71	109777.0
72	78924.0
73	60965.5
74	43007.0
75	31962.5
76	20918.0
77	15661.5
78	10405.0
79	7415.5
80	4426.0
81	3138.5
82	1851.0
83	1438.0
84	1025.0
85	797.0
86	569.0
87	417.0
88	265.0
89	217.0
90	169.0
91	167.0
92	165.0
93	147.0
94	129.0
95	131.5
96	134.0
97	121.5
98	109.0
99	107.0
100	105.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.24321504624795165
2	9.318012012888603E-4
3	5.97308462364654E-6
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.040637881236979236
10	0.0
11	0.0
12	0.0
13	0.0
14	1.493271155911635E-5
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	8.063664241922829E-5
21	8.362318473105156E-5
22	5.106987353217791E-4
23	4.0616975440796476E-4
24	4.479813467734905E-5
25	0.0070452533135910945
26	0.012373244797883806
27	0.019767923561958226
28	0.02704911371818336
29	0.022198969003782366
30	0.02430448133361777
31	0.03317152545742106
32	0.03927601794278782
33	0.05089665407809217
34	0.05698621385189981
35	0.0543013123135707
36	0.053778667409001626
37	0.029133720251836002
38	0.01743543401642425
39	0.010429005752886859
40	0.014069600830999427
41	0.0032314387813927783
42	0.006277711939452513
43	0.029886328914415464
44	0.05764325316050094
45	0.07450825759536694
46	0.07485170996122663
47	0.07697215500262114
48	0.07791590237315729
49	0.07477107331880739
50	0.07625239830547173
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3.3483537E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.489885462915026
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.92021642697995	32.442120767673124
2	14.422606579433895	12.833202295913972
3	4.946649670371374	6.6022763177996655
4	2.346539594076601	4.175891110986525
5	1.263078111379517	2.8097100252994873
6	0.7728564696534026	2.063057748849165
7	0.5274108958948254	1.642511524517793
8	0.39368538108846846	1.4012014010440013
9	0.2741099550747848	1.0975608454969765
>10	1.7832602960003041	15.653565610101408
>50	0.22349600975539174	6.9737078351194635
>100	0.11920825289660988	8.266630104289858
>500	0.003235973751685949	1.0886444907276278
>1k	0.0036127599330770623	2.2533261598693306
>5k	6.7247421600608684E-6	0.01618879258824373
>10k+	2.6898967850900822E-5	0.6804049697234092
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG	157562	0.4705655797354981	Illumina Paired End PCR Primer 2 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	39671	0.11847912005234094	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.570393086011194E-4	0.0	0.0	0.0	0.0
2	6.689854778484125E-4	0.0	0.0	2.98654231182327E-6	0.0
3	6.779451047838823E-4	0.0	0.0	2.98654231182327E-6	0.0
4	7.018374432784685E-4	0.0	0.0	5.97308462364654E-6	0.0
5	8.780434396760414E-4	0.0	0.0	1.194616924729308E-5	0.0
6	8.840165242996879E-4	0.0	0.0	1.493271155911635E-5	0.0
7	9.049223204824509E-4	0.0	0.0	2.389233849458616E-5	0.0
8	0.001051262893761791	0.0	0.0	2.389233849458616E-5	0.0
9	0.0013857556326859974	0.0	0.0	2.687888080640943E-5	0.0
10	0.002401180018705909	0.0	0.0	2.687888080640943E-5	0.0
11	0.0025057089996197235	0.0	0.0	3.285196543005597E-5	0.0
12	0.0026819149960172965	0.0	0.0	3.285196543005597E-5	0.0
13	0.002753592011501055	0.0	0.0	3.285196543005597E-5	0.0
14	0.002816309400049344	0.0	0.0	3.285196543005597E-5	0.0
15	0.004999471829992154	0.0	0.0	3.882505005370251E-5	0.0
16	0.005109973895529615	0.0	0.0	3.882505005370251E-5	0.0
17	0.005495237853754817	0.0	0.0	3.882505005370251E-5	0.0
18	0.005740134323324325	0.0	0.0	4.181159236552578E-5	0.0
19	0.006146304077732289	0.0	0.0	4.181159236552578E-5	0.0
20	0.05768805129517828	0.0	0.0	4.778467698917232E-5	0.0
21	0.06044164330667934	0.0	0.0	4.778467698917232E-5	0.0
22	0.06077314950329172	0.0	0.0	5.375776161281886E-5	0.0
23	0.06119723851157063	0.0	0.0	5.375776161281886E-5	0.0
24	0.06268752312517044	0.0	0.0	5.375776161281886E-5	0.0
25	0.06296825810248183	0.0	0.0	5.375776161281886E-5	0.0
26	0.06371489368043765	0.0	0.0	5.674430392464213E-5	0.0
27	0.06398965557312539	0.0	0.0	5.97308462364654E-5	0.0
28	0.06529776110570398	0.0	0.0	5.97308462364654E-5	0.0
29	0.066328118203283	0.0	0.0	5.97308462364654E-5	0.0
30	0.07343310236311057	0.0	0.0	6.570393086011194E-5	0.0
31	0.5939993734831538	0.0	0.0	6.570393086011194E-5	0.0
32	0.5983119405814267	0.0	0.0	6.869047317193521E-5	0.0
33	0.599724575094919	0.0	0.0	7.167701548375848E-5	0.0
34	0.6000232293261014	0.0	0.0	7.167701548375848E-5	0.0
35	0.6003188970149719	0.0	0.0	7.466355779558175E-5	0.0
36	0.6005966454499714	0.0	0.0	7.765010010740502E-5	0.0
37	0.6008833535119065	0.0	0.0	9.556935397834463E-5	0.0
38	0.6012447251316371	0.0	0.0	9.556935397834463E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGCCGA	25155	0.0	34.360126	25
TGCCGAG	25310	0.0	34.134666	26
AGATCGG	23415	0.0	33.258266	31
AATGCCG	26220	0.0	33.08896	24
CGAGATC	25215	0.0	30.85717	29
CCGAGAT	25560	0.0	30.586473	28
GAGATCG	25905	0.0	30.180206	30
GCCGAGA	28940	0.0	29.985144	27
GTTCAGC	34470	0.0	25.626059	14
GAATGCC	35275	0.0	24.93145	23
GGAATGC	36290	0.0	24.421354	22
TATGCCG	3090	0.0	24.004017	43
CGTATGC	3145	0.0	23.723597	41
TCGTATG	3115	0.0	23.527546	40
CAGGAAT	38350	0.0	23.306221	20
GACCGAT	3210	0.0	22.827154	32
CTCGTAT	3275	0.0	22.443933	39
AGGAATG	40500	0.0	22.141258	21
ACCGATC	3445	0.0	21.781094	33
GCAGGAA	42375	0.0	21.340132	19
>>END_MODULE