FastQCFastQC Report
Thu 26 May 2016
SRR522089_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522089_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30082761
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG693010.23036781763482417Illumina Paired End PCR Primer 2 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT386100.12834593207717868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG119750.031.34608831
ATGCCGA137400.030.69111425
AATGCCG142550.029.59627324
TGCCGAG146400.028.92615926
GAGATCG140250.026.82701730
CGAGATC140300.026.80168329
CCGAGAT153400.024.74142828
GCCGAGA176950.024.12061327
GATCGGA212450.020.110521
GTTCAGC215200.020.04755814
GACCGAT23650.019.81656332
GCGGTTC217750.019.60072311
GGAATGC224000.019.48124922
GAATGCC219850.019.47972523
TCGTATG22850.019.45443540
GAGCGGT222250.019.2681569
CGGTTCA221750.019.2669912
AGAGCGG227150.019.1915848
ATCGGAA226800.019.0223882
TATGCCG22450.019.02018543
Produced by FastQC (version 0.11.5)