##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR522089_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 30082761 Sequences flagged as poor quality 0 Sequence length 50 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.42500909407883 39.0 37.0 40.0 33.0 40.0 2 36.71291511440722 39.0 36.0 40.0 31.0 40.0 3 36.930768090069925 39.0 36.0 40.0 31.0 40.0 4 36.90222446669706 39.0 36.0 40.0 31.0 40.0 5 36.75168296553631 39.0 36.0 40.0 31.0 40.0 6 36.84240858078153 39.0 36.0 40.0 31.0 40.0 7 36.79106279506725 39.0 36.0 40.0 31.0 40.0 8 36.948771191580455 39.0 36.0 40.0 31.0 40.0 9 36.84615335008645 38.0 36.0 40.0 31.0 40.0 10 36.72251004487254 38.0 36.0 40.0 31.0 40.0 11 36.80380780208306 39.0 36.0 40.0 31.0 40.0 12 36.963558896738235 39.0 36.0 40.0 31.0 40.0 13 36.88483294468882 39.0 36.0 40.0 31.0 40.0 14 36.95789508815364 38.0 36.0 40.0 31.0 40.0 15 36.983823559280346 38.0 36.0 40.0 32.0 40.0 16 37.09057393368913 39.0 36.0 40.0 33.0 40.0 17 36.95498282222167 39.0 36.0 40.0 32.0 40.0 18 36.95227432747945 39.0 36.0 40.0 31.0 40.0 19 36.866748002286094 38.0 36.0 40.0 31.0 40.0 20 36.74699037764519 38.0 36.0 40.0 31.0 40.0 21 36.88488350520752 39.0 36.0 40.0 32.0 40.0 22 36.73663348254504 38.0 36.0 40.0 31.0 40.0 23 36.657719116938765 38.0 35.0 40.0 31.0 40.0 24 36.77726612261421 38.0 36.0 40.0 31.0 40.0 25 36.78161379535609 38.0 36.0 40.0 31.0 40.0 26 36.69418142836025 38.0 36.0 40.0 31.0 40.0 27 36.537804791255695 38.0 35.0 40.0 31.0 40.0 28 36.53723173880217 38.0 35.0 40.0 31.0 40.0 29 36.37933027490396 38.0 35.0 40.0 31.0 40.0 30 36.43975032743836 38.0 35.0 40.0 31.0 40.0 31 36.42620280099955 38.0 35.0 40.0 31.0 40.0 32 36.307172370248864 38.0 35.0 40.0 30.0 40.0 33 36.24131804258259 38.0 35.0 40.0 30.0 40.0 34 36.129944721496805 38.0 35.0 40.0 30.0 40.0 35 36.18606939037278 38.0 35.0 40.0 30.0 40.0 36 36.228396256580304 38.0 35.0 40.0 30.0 40.0 37 36.06146490343755 38.0 35.0 40.0 30.0 40.0 38 35.94991809428662 38.0 35.0 40.0 29.0 40.0 39 35.833526982446855 38.0 35.0 40.0 29.0 40.0 40 35.632396873411984 38.0 35.0 39.0 29.0 40.0 41 35.63210511162855 38.0 35.0 40.0 29.0 40.0 42 35.76398845172489 38.0 35.0 40.0 29.0 40.0 43 35.84206948291747 38.0 35.0 40.0 29.0 40.0 44 35.885924799256294 38.0 35.0 40.0 30.0 40.0 45 35.74254504099541 38.0 35.0 40.0 29.0 40.0 46 35.94209175148518 38.0 35.0 40.0 30.0 40.0 47 35.76800570266805 38.0 35.0 40.0 30.0 40.0 48 35.63847473973549 38.0 35.0 40.0 29.0 40.0 49 35.563079964634895 38.0 35.0 40.0 29.0 40.0 50 35.38357652743377 38.0 35.0 40.0 29.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2 1 0.08101180297235544 2 2 0.4509152451525722 2 3 0.10135592210596656 2 4 0.2055711256453563 2 5 0.3870141563538425 2 6 0.348209186372884 2 7 0.23848558533786246 2 8 0.16576250399194947 2 9 0.15373973522009265 2 10 0.35595669251218 2 11 0.3011917309128478 2 12 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0.14390238542745237 120 21 0.11655905360085228 120 22 0.15441638563977733 120 23 0.20924907607057008 120 24 -0.0011608228766277762 120 25 -0.011929457227530804 120 26 -0.015365400223295467 120 27 0.09634856764268562 120 28 -0.08084015189910332 120 29 0.04468816026362532 120 30 -0.055145028695001486 120 31 -0.03363287372088308 120 32 -0.067230018107999 120 33 -0.07802312114360177 120 34 0.017473868572402296 120 35 0.17438744254890537 120 36 0.23906434868824533 120 37 0.22205576997316712 120 38 0.28796405889511334 120 39 0.3574738751544615 120 40 0.36194519247947454 120 41 0.6954853553177784 120 42 0.4092412827781402 120 43 0.410383950186727 120 44 0.2581229594520096 120 45 0.3017733153212063 120 46 0.2704431951758366 120 47 0.2073985917745773 120 48 0.249686100511596 120 49 0.3259252571569746 120 50 0.36615804438314115 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 28731.0 3 1571.0 4 1103.0 5 1231.0 6 1874.0 7 2830.0 8 3262.0 9 4257.0 10 5834.0 11 7380.0 12 10008.0 13 13304.0 14 16484.0 15 20212.0 16 23030.0 17 26653.0 18 31038.0 19 35348.0 20 40407.0 21 46873.0 22 57768.0 23 73509.0 24 97743.0 25 135163.0 26 189585.0 27 265195.0 28 359564.0 29 470964.0 30 606562.0 31 767414.0 32 968684.0 33 1232611.0 34 1614524.0 35 2190045.0 36 2998870.0 37 4221746.0 38 4774573.0 39 8713657.0 40 23154.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 13.40647641559479 28.57890973580916 30.293344587994298 27.72126926060175 2 23.137609351058096 31.036245721569472 25.114088433557157 20.712056493815275 3 23.168106145576196 28.955540350834152 25.705482950850154 22.17087055273949 4 23.742132578854715 26.795007280083098 26.362011784756064 23.100848356306123 5 25.12768359260641 28.042106906344134 27.308723424688313 19.521486076361143 6 25.188811625369095 27.353061110314975 23.950328229513243 23.50779903480269 7 24.269883339497994 26.96475566188888 27.550935234967294 21.21442576364583 8 23.59489875281062 27.85734660458859 26.36853711665628 22.179217525944512 9 22.754643631355083 26.833860705065078 27.58926856356369 22.82222710001615 10 22.68451024159651 28.240582704493118 25.881543918126397 23.19336313578398 11 23.424802112572117 27.326897532008367 26.20808064153688 23.040219713882635 12 22.473768282106818 27.215005298217143 27.08018389668422 23.231042522991824 13 23.210891447098223 27.0683831181586 26.69524582534163 23.025479609401543 14 23.252240710219386 27.50922696224592 26.804627407703702 22.433904919830997 15 22.51908991997111 27.58183665388958 27.086313653191606 22.812759772947704 16 22.441380962339196 27.397701294771448 27.31732303427867 22.84359470861069 17 23.004563976026006 27.093410741121797 27.003093233363785 22.898932049488412 18 22.36034119341639 27.471235103719373 27.085402832539206 23.083020870325036 19 23.000275805801206 27.432322452051526 26.963638743132652 22.603762999014616 20 23.10009121503479 27.040750250309483 27.0333373666512 22.82582116800453 21 22.623876049555605 27.699532077073318 26.95238584048816 22.72420603288292 22 23.36504975362114 26.915032825306607 26.86837815777056 22.85153926330169 23 23.33050102363048 26.985523507865818 27.08240664971048 22.601568818793222 24 22.624027096449026 27.516806053806032 26.99532134035171 22.863845509393236 25 22.789678485088434 27.245511550077666 26.940104598681003 23.024705366152897 26 23.050899905342412 27.07897211558897 27.33938105113508 22.53074692793354 27 23.356940627654783 26.95058261804894 27.002595139889845 22.689881614406435 28 22.966716457735362 27.008806951132435 26.938946507434995 23.085530083697208 29 23.246240666505287 27.00278774562353 26.89895220133482 22.85201938653636 30 23.23108926531385 26.982552541447046 27.025894709023596 22.760463484215503 31 22.870116416907962 27.288999404785088 27.019647944958898 22.821236233348056 32 23.3370401432886 26.963154929802286 26.963959697753104 22.735845229156013 33 22.898021800028104 27.328724102176928 26.889593808386252 22.88366028940872 34 22.924452479676948 27.05203541829257 27.18744370599422 22.836068396036264 35 23.18748154791674 27.03409313008401 26.843255847555824 22.935169474443423 36 23.28177867849483 26.984492030968845 26.896221804146194 22.837507486390134 37 23.24011384263035 27.03948677247604 26.848232802745827 22.87216658214778 38 23.087063796905653 27.39978423631114 26.8686966344515 22.644455332331702 39 23.02143975712657 27.270710822671578 26.83970822342454 22.868141196777316 40 23.23901173228639 26.998145364018438 26.914602633619 22.848240270076168 41 23.361719172107794 27.30924860285619 26.70711870588845 22.62191351914757 42 23.39775001860817 26.981741984798248 26.790583956162706 22.82992404043088 43 23.048460167712552 26.983974872345534 26.883010439896555 23.084554520045366 44 23.26038458371133 27.12822618915056 26.877206685374478 22.734182541763634 45 23.24742601905773 27.009199462561025 26.887447691465134 22.85592682691611 46 22.91264984540377 26.98720067304124 27.24521644885916 22.854933032695826 47 23.143244107085604 27.01604273235642 27.127086893811313 22.71362626674666 48 22.99522693439186 26.98708543279701 26.788794957813533 23.2288926749976 49 22.924031748220074 27.197331577113754 26.92828292452612 22.95035375014006 50 23.412768345225224 26.99192346409362 26.81264598566798 22.782662205013178 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2405.0 1 1505.0 2 605.0 3 510.0 4 415.0 5 437.0 6 459.0 7 635.0 8 811.0 9 1401.5 10 1992.0 11 3756.5 12 5521.0 13 9393.5 14 13266.0 15 21501.5 16 29737.0 17 46117.0 18 62497.0 19 91579.5 20 120662.0 21 165850.5 22 211039.0 23 288186.0 24 365333.0 25 408932.0 26 452531.0 27 534673.0 28 616815.0 29 703380.0 30 789945.0 31 897528.5 32 1005112.0 33 1121896.5 34 1238681.0 35 1369633.0 36 1500585.0 37 1603811.0 38 1707037.0 39 1801182.5 40 1895328.0 41 1959631.0 42 2023934.0 43 2079331.5 44 2134729.0 45 2145186.5 46 2155644.0 47 2108483.0 48 2061322.0 49 2016467.0 50 1971612.0 51 1912554.5 52 1853497.0 53 1766872.5 54 1680248.0 55 1564209.5 56 1448171.0 57 1388971.0 58 1329771.0 59 1196038.0 60 1062305.0 61 929221.5 62 796138.0 63 681951.5 64 567765.0 65 478190.0 66 388615.0 67 319929.5 68 251244.0 69 202384.5 70 153525.0 71 121492.0 72 89459.0 73 68984.0 74 48509.0 75 36229.0 76 23949.0 77 17589.0 78 11229.0 79 8076.0 80 4923.0 81 3556.0 82 2189.0 83 1625.0 84 1061.0 85 802.5 86 544.0 87 462.5 88 381.0 89 298.5 90 216.0 91 219.5 92 223.0 93 228.0 94 233.0 95 219.5 96 206.0 97 205.0 98 204.0 99 171.5 100 139.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20811254658440426 2 5.850526818332931E-4 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.07856659167687434 10 0.0 11 3.3241629649618925E-6 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 3.3241629649618925E-6 21 9.64007259838949E-5 22 8.642823708900923E-5 23 4.2549285951512224E-4 24 0.0 25 0.01071377723607218 26 0.009187986435154671 27 0.0243029554368364 28 0.023555018769719972 29 0.027527393512849437 30 0.027002175764385457 31 0.037463316615120534 32 0.03982679648320844 33 0.05413731804736939 34 0.0578171664495822 35 0.061181219370123635 36 0.04941368247415854 37 0.028634339780181747 38 0.01490554673488913 39 0.009626775946529642 40 0.011777509384859986 41 0.004028885513533814 42 0.006282668003777978 43 0.0292659307435245 44 0.05376168763232869 45 0.07452108534851572 46 0.07378312117029417 47 0.084267531161784 48 0.09262115269273322 49 0.08063422104108063 50 0.08458000248049041 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 3.0082761E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.3442593387473 #Duplication Level Percentage of deduplicated Percentage of total 1 76.27884800027167 36.11365561785748 2 12.32432725710517 11.669722916719588 3 4.262746022733057 6.054496595864623 4 1.9824867495152845 3.7543746721872715 5 1.1517527432095978 2.726444028431441 6 0.7081822261431676 2.0117017784168114 7 0.5149101229477784 1.7064626877890114 8 0.3653711392579029 1.3838580777535767 9 0.2803160066775168 1.1944218345248139 >10 1.855662110412692 16.912615937726187 >50 0.16575583265617905 5.417624015075991 >100 0.10645859373471504 9.648433932525801 >500 0.0030259936771067646 0.887023318650549 >1k 1.1484865335250291E-4 0.07063108351839117 >5k 2.823533665676303E-5 0.08790004121145516 >10k+ 1.411766825868527E-5 0.36063346174715466 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG 69301 0.23036781763482417 Illumina Paired End PCR Primer 2 (97% over 36bp) AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 38610 0.12834593207717868 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.019486077092459E-4 3.324162964961893E-6 0.0 3.324162964961893E-6 0.0 2 5.052727706742077E-4 6.648325929923786E-6 0.0 3.324162964961893E-6 0.0 3 5.119210966041315E-4 6.648325929923786E-6 0.0 3.324162964961893E-6 0.0 4 5.385144003238266E-4 6.648325929923786E-6 0.0 3.324162964961893E-6 0.0 5 5.983493336931407E-4 6.648325929923786E-6 0.0 3.324162964961893E-6 0.0 6 6.016734966581026E-4 6.648325929923786E-6 3.324162964961893E-6 3.324162964961893E-6 0.0 7 6.049976596230645E-4 6.648325929923786E-6 3.324162964961893E-6 6.648325929923786E-6 0.0 8 6.947500596770356E-4 6.648325929923786E-6 3.324162964961893E-6 6.648325929923786E-6 0.0 9 8.277165782755114E-4 6.648325929923786E-6 3.324162964961893E-6 6.648325929923786E-6 0.0 10 0.0014127692601088044 6.648325929923786E-6 3.324162964961893E-6 9.97248889488568E-6 0.0 11 0.0014859008453379661 9.97248889488568E-6 3.324162964961893E-6 9.97248889488568E-6 0.0 12 0.0016088948750415562 9.97248889488568E-6 3.324162964961893E-6 9.97248889488568E-6 0.0 13 0.0016521089935860609 1.3296651859847572E-5 3.324162964961893E-6 9.97248889488568E-6 0.0 14 0.0016919989491656034 1.6620814824809466E-5 3.324162964961893E-6 9.97248889488568E-6 0.0 15 0.0030914715574145604 1.6620814824809466E-5 3.324162964961893E-6 9.97248889488568E-6 0.0 16 0.003194520609328379 1.994497778977136E-5 3.324162964961893E-6 9.97248889488568E-6 0.0 17 0.003536909394719454 2.326914075473325E-5 3.324162964961893E-6 1.3296651859847572E-5 0.0 18 0.003749655824477015 2.6593303719695143E-5 3.324162964961893E-6 1.3296651859847572E-5 0.0 19 0.003998968046849157 2.6593303719695143E-5 3.324162964961893E-6 1.3296651859847572E-5 0.0 20 0.036898208911077014 2.6593303719695143E-5 3.324162964961893E-6 1.3296651859847572E-5 0.0 21 0.03864339446768201 2.6593303719695143E-5 3.324162964961893E-6 1.3296651859847572E-5 0.0 22 0.038959189949353386 2.9917466684657036E-5 3.324162964961893E-6 1.3296651859847572E-5 0.0 23 0.039255040453234996 3.324162964961893E-5 3.324162964961893E-6 1.6620814824809466E-5 0.0 24 0.04013594363894989 3.324162964961893E-5 3.324162964961893E-6 1.6620814824809466E-5 0.0 25 0.0403619867205673 3.324162964961893E-5 3.324162964961893E-6 1.6620814824809466E-5 0.0 26 0.04077750709118754 3.324162964961893E-5 3.324162964961893E-6 1.6620814824809466E-5 0.0 27 0.040993577683910064 3.324162964961893E-5 3.324162964961893E-6 1.994497778977136E-5 0.0 28 0.04199082657339863 3.656579261458082E-5 3.324162964961893E-6 1.994497778977136E-5 0.0 29 0.04265565916639101 3.656579261458082E-5 3.324162964961893E-6 1.994497778977136E-5 0.0 30 0.04655490232429131 3.988995557954272E-5 3.324162964961893E-6 2.326914075473325E-5 0.0 31 0.31463202463364315 3.988995557954272E-5 3.324162964961893E-6 2.326914075473325E-5 3.324162964961893E-6 32 0.3170719602499252 3.988995557954272E-5 3.324162964961893E-6 2.326914075473325E-5 3.324162964961893E-6 33 0.31772682035402267 4.65382815094665E-5 3.324162964961893E-6 2.326914075473325E-5 3.324162964961893E-6 34 0.3178996768282007 4.65382815094665E-5 3.324162964961893E-6 2.326914075473325E-5 3.324162964961893E-6 35 0.31816560986539766 4.986244447442839E-5 3.324162964961893E-6 2.6593303719695143E-5 3.324162964961893E-6 36 0.3184415153914895 5.318660743939029E-5 3.324162964961893E-6 2.9917466684657036E-5 3.324162964961893E-6 37 0.3187772558509506 5.318660743939029E-5 3.324162964961893E-6 2.9917466684657036E-5 3.324162964961893E-6 38 0.31914291377709647 5.318660743939029E-5 3.324162964961893E-6 3.656579261458082E-5 3.324162964961893E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGATCGG 11975 0.0 31.346088 31 ATGCCGA 13740 0.0 30.691114 25 AATGCCG 14255 0.0 29.596273 24 TGCCGAG 14640 0.0 28.926159 26 GAGATCG 14025 0.0 26.827017 30 CGAGATC 14030 0.0 26.801683 29 CCGAGAT 15340 0.0 24.741428 28 GCCGAGA 17695 0.0 24.120613 27 GATCGGA 21245 0.0 20.11052 1 GTTCAGC 21520 0.0 20.047558 14 GACCGAT 2365 0.0 19.816563 32 GCGGTTC 21775 0.0 19.600723 11 GGAATGC 22400 0.0 19.481249 22 GAATGCC 21985 0.0 19.479725 23 TCGTATG 2285 0.0 19.454435 40 GAGCGGT 22225 0.0 19.268156 9 CGGTTCA 22175 0.0 19.26699 12 AGAGCGG 22715 0.0 19.191584 8 ATCGGAA 22680 0.0 19.022388 2 TATGCCG 2245 0.0 19.020185 43 >>END_MODULE