SRR522088_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR522088_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27666026
Sequences flagged as poor quality	0
Sequence length	50
%GC	42

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	238845	0.863315172189891	Illumina PCR Primer Index 1 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	212878	0.7694563722306919	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	202804	0.7330434808381948	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	125177	0.452457465340342	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	74892	0.27070024440806933	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	68998	0.24939613661897087	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	58642	0.21196394451447417	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	37591	0.13587423072616212	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	67275	36.300694	1
CGTCTTC	50080	36.08614	40
GCCGTCT	51245	35.439663	38
CCGTCTT	51240	35.438446	39
TATGCCG	51620	35.064785	35
TGCCGTC	52690	34.382263	37
ATGCCGT	52905	34.288593	36
GACCGAT	56050	32.166626	24
TCGTATG	56180	32.119606	32
TGAGCGG	60145	31.991396	5
ATATCGT	34570	31.950512	29
CGTATGC	56820	31.882763	33
GCGGGCT	60160	31.83383	8
AGACCGA	58700	31.623781	23
CGGGCTG	60830	31.50485	9
TATCGTA	35075	31.484589	30
ATCGTAT	35520	31.44554	31
AGCGGGC	61080	31.426214	7
CTGAGCG	61435	31.355146	4
CGATATC	35580	31.285147	27