SRR522087_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR522087_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28866226
Sequences flagged as poor quality	0
Sequence length	50
%GC	44

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Sequence	Count	Percentage	Possible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	129480	0.4485518820506706	Illumina PCR Primer Index 1 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	105498	0.3654720918487924	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	99771	0.345632297065782	No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT	74969	0.2597118168478276	Illumina Paired End PCR Primer 2 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	62657	0.21705989553327823	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34368	0.11905955423476558	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33968	0.1176738517878991	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28910	0.10015164434727282	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	27560	36.201824	35
GACCGAT	28090	35.268734	24
TCGTATG	28345	35.143616	32
GCCGTCT	28565	35.03295	38
CGTATGC	28590	35.01673	33
CCGTCTT	28630	34.960434	39
TATCGTA	17190	34.959503	30
ATATCGT	17220	34.872803	29
ATCGTAT	17980	34.023212	31
ACCGATA	17965	33.999268	25
ATGCCGT	29430	33.96892	36
CGTCTTC	29525	33.896553	40
GCGGGCT	30985	33.887432	8
TGAGCGG	31150	33.863262	5
AGACCGA	30115	33.861526	23
GATATCG	18090	33.66563	28
CGATATC	18100	33.658543	27
TGCCGTC	29905	33.488266	37
CCGATAT	18280	33.26901	26
CGGGCTG	32095	32.729145	9