FastQCFastQC Report
Thu 26 May 2016
SRR522087_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522087_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28866226
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT1294800.4485518820506706Illumina PCR Primer Index 1 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1054980.3654720918487924No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT997710.345632297065782No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT749690.2597118168478276Illumina Paired End PCR Primer 2 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT626570.21705989553327823No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT343680.11905955423476558No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT339680.1176738517878991No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289100.10015164434727282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG275600.036.20182435
GACCGAT280900.035.26873424
TCGTATG283450.035.14361632
GCCGTCT285650.035.0329538
CGTATGC285900.035.0167333
CCGTCTT286300.034.96043439
TATCGTA171900.034.95950330
ATATCGT172200.034.87280329
ATCGTAT179800.034.02321231
ACCGATA179650.033.99926825
ATGCCGT294300.033.9689236
CGTCTTC295250.033.89655340
GCGGGCT309850.033.8874328
TGAGCGG311500.033.8632625
AGACCGA301150.033.86152623
GATATCG180900.033.6656328
CGATATC181000.033.65854327
TGCCGTC299050.033.48826637
CCGATAT182800.033.2690126
CGGGCTG320950.032.7291459