##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522087_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28866226
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.950477731311324	40.0	39.0	40.0	38.0	40.0
2	38.81413008406433	40.0	39.0	40.0	37.0	40.0
3	38.775619992720905	40.0	39.0	40.0	36.0	40.0
4	38.83359196314752	40.0	39.0	40.0	37.0	40.0
5	38.82969623393096	40.0	39.0	40.0	37.0	40.0
6	38.90373680993144	40.0	39.0	40.0	38.0	40.0
7	38.856891233374256	40.0	39.0	40.0	37.0	40.0
8	38.839706201981514	40.0	39.0	40.0	37.0	40.0
9	38.86597721503323	40.0	39.0	40.0	37.0	40.0
10	38.80674591822291	40.0	39.0	40.0	37.0	40.0
11	38.89255491867901	40.0	39.0	40.0	37.0	40.0
12	38.822691681274854	40.0	39.0	40.0	36.0	40.0
13	38.82569099957854	40.0	39.0	40.0	36.0	40.0
14	38.79774588475819	40.0	39.0	40.0	36.0	40.0
15	38.77156310630978	40.0	39.0	40.0	36.0	40.0
16	38.748299933631785	40.0	39.0	40.0	36.0	40.0
17	38.697436720685275	40.0	39.0	40.0	36.0	40.0
18	38.68510123214583	40.0	39.0	40.0	36.0	40.0
19	38.64425280256588	40.0	39.0	40.0	36.0	40.0
20	38.622019449303835	40.0	39.0	40.0	36.0	40.0
21	38.58323433066727	40.0	39.0	40.0	36.0	40.0
22	38.514864083721925	40.0	38.0	40.0	35.0	40.0
23	38.47122294407312	40.0	38.0	40.0	35.0	40.0
24	38.41703695522927	40.0	38.0	40.0	35.0	40.0
25	38.37528650264153	40.0	38.0	40.0	35.0	40.0
26	38.24501886737809	40.0	38.0	40.0	35.0	40.0
27	38.16507145755735	40.0	38.0	40.0	35.0	40.0
28	38.10442584354463	40.0	38.0	40.0	35.0	40.0
29	38.01313808739667	40.0	38.0	40.0	35.0	40.0
30	38.09697305078953	40.0	38.0	40.0	35.0	40.0
31	37.91738826544211	40.0	38.0	40.0	35.0	40.0
32	37.922964332088306	40.0	38.0	40.0	35.0	40.0
33	37.8585731643617	40.0	38.0	40.0	34.0	40.0
34	37.80550609560114	40.0	38.0	40.0	34.0	40.0
35	37.68574651913278	40.0	38.0	40.0	33.0	40.0
36	37.93290304039052	40.0	38.0	40.0	35.0	40.0
37	37.86066207615779	40.0	38.0	40.0	35.0	40.0
38	37.79129706806841	40.0	38.0	40.0	35.0	40.0
39	37.70542404815926	40.0	38.0	40.0	34.0	40.0
40	37.62914317237037	40.0	38.0	40.0	34.0	40.0
41	37.570481122125216	40.0	38.0	40.0	34.0	40.0
42	37.46234169995066	40.0	38.0	40.0	33.0	40.0
43	37.37908100629434	40.0	38.0	40.0	33.0	40.0
44	37.25812134222188	40.0	38.0	40.0	33.0	40.0
45	37.12240567921834	40.0	38.0	40.0	33.0	40.0
46	36.85104737973021	40.0	38.0	40.0	33.0	40.0
47	36.741202504269175	40.0	38.0	40.0	33.0	40.0
48	36.630821535174015	40.0	38.0	40.0	32.0	40.0
49	36.51498606017981	39.0	37.0	40.0	32.0	40.0
50	36.44033473582587	39.0	37.0	40.0	32.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1	1	0.020785285743080806
1	2	-0.018621080397721812
1	3	-0.016866323650539528
1	4	-0.04059828484481898
1	5	-0.00494019049200034
1	6	-0.021638772458373978
1	7	-0.015754040700834082
1	8	-0.028538766609315758
1	9	-0.04099409751717786
1	10	-0.04234944299446397
1	11	-0.04045698824773325
1	12	-0.06821264941915928
1	13	-0.057667882771440304
1	14	-0.0671439544084862
1	15	-0.07003530019064641
1	16	-0.055365391574071054
1	17	-0.0616530603219374
1	18	-0.08374841957829915
1	19	-0.05407759624436892
1	20	-0.07556268951392298
1	21	-0.09044928821864318
1	22	-0.06012874181011796
1	23	-0.06761430884508002
1	24	-0.07053591305350437
1	25	-0.07190833084852954
1	26	-0.0722632055695911
1	27	-0.0732319893598401
1	28	-0.08635728273665677
1	29	-0.07291683323800413
1	30	-0.04412832373272835
1	31	0.033239019036329864
1	32	0.033638299297905405
1	33	0.053363052322779936
1	34	0.04649442618300981
1	35	0.052630331965708876
1	36	-0.024420847986938554
1	37	-0.060152838765873184
1	38	-0.030046655464090577
1	39	-0.03968356380258342
1	40	0.01291368231398593
1	41	-0.04519926255065343
1	42	-0.030719463586883933
1	43	-0.04656341991634605
1	44	-0.06970459390434769
1	45	-0.06495734567065625
1	46	-0.0920330720296505
1	47	-0.06129715299635308
1	48	-0.07421075616836248
1	49	-0.14578148636692134
1	50	-0.049540333566710615
2	1	-0.21688640677733417
2	2	-0.4600682589760936
2	3	-0.40653992105661274
2	4	-0.3517624403058406
2	5	-0.2545768188917492
2	6	-0.2596081587805017
2	7	-0.3088631831401045
2	8	-0.261508579169508
2	9	-0.30180210635305826
2	10	-0.296367289621827
2	11	-0.25093983773619044
2	12	-0.2569569064975852
2	13	-0.2594363336481109
2	14	-0.27804654302964593
2	15	-0.26072943993467845
2	16	-0.23402189451795863
2	17	-0.20283963073098477
2	18	-0.2151315067022992
2	19	-0.20640254389146406
2	20	-0.20632581201839173
2	21	-0.19228217500344869
2	22	-0.17847929240725335
2	23	-0.17527546391768567
2	24	-0.18106208474866037
2	25	-0.15737976026552047
2	26	-0.19462075646450927
2	27	-0.18112084693981245
2	28	-0.18007885187022765
2	29	-0.21768291408081808
2	30	-0.16574652996898465
2	31	-0.1341494383951769
2	32	-0.1680475953370859
2	33	-0.17457050350557068
2	34	-0.170053091506027
2	35	-0.16423036273223346
2	36	-0.15325561097030516
2	37	-0.1922383398264529
2	38	-0.17794712213739672
2	39	-0.1995832995913105
2	40	-0.1511327468823751
2	41	-0.15439727915207158
2	42	-0.17002935297132638
2	43	-0.20237331490260146
2	44	-0.1853494351375531
2	45	-0.18985346448621243
2	46	-0.2018319163507556
2	47	-0.16979764811473785
2	48	-0.18083484148557716
2	49	-0.20380846291763532
2	50	-0.2046517942987407
4	1	-0.015626117220875813
4	2	-0.015559746910213335
4	3	-0.04021552778063153
4	4	-0.027317082157246375
4	5	-0.012262094853113581
4	6	-0.03251854230616402
4	7	-0.035816327243360035
4	8	-0.0213225357295741
4	9	-0.04421757287349237
4	10	-0.038193835503697926
4	11	-0.04547949484083347
4	12	-0.05438708114306223
4	13	-0.06819852760628464
4	14	-0.07224624902465138
4	15	-0.06756384622582345
4	16	-0.2747661403198123
4	17	-1.6921874373805394
4	18	-0.26796470335159484
4	19	-1.748724625486581
4	20	-0.26482743567218847
4	21	-0.4916784446931928
4	22	-0.9103768736348954
4	23	-1.3416430306019151
4	24	-1.3223164391226803
4	25	-0.9090074127030405
4	26	-0.9962399923432415
4	27	-1.4072467629962162
4	28	-0.43127204576916256
4	29	-0.4460007097973815
4	30	-0.4330168943171344
4	31	-0.09439383322269634
4	32	-0.11305686658477043
4	33	-0.10650065674916931
4	34	-0.09835677883836524
4	35	-0.06323151863366405
4	36	-0.04489334830425662
4	37	-0.04129237707323341
4	38	-0.05239540245574403
4	39	-0.018255062572322345
4	40	-0.0066674401140645045
4	41	-0.03964160271392103
4	42	0.021990166756623353
4	43	0.017532421191852166
4	44	0.01053084263018178
4	45	7.725133023441799E-4
4	46	0.010318161307750984
4	47	0.0028817872229041086
4	48	-0.03963400568845543
4	49	-0.04399429172866576
4	50	-0.05133477418559096
5	1	-0.0029243146822821586
5	2	-0.0320911289726169
5	3	-0.027255197047125534
5	4	-0.025420869943175717
5	5	-0.00529153871876531
5	6	-0.021648876824770014
5	7	-0.037872308892175965
5	8	-0.03205287380780675
5	9	-0.029197249748307286
5	10	-0.03089733817109419
5	11	-0.055085392210216355
5	12	-0.05019592074405921
5	13	-0.040352342278069386
5	14	-0.05208835355028185
5	15	-0.07065757569669984
5	16	-0.06111935096951271
5	17	-0.03477473112174323
5	18	-0.06054033271424686
5	19	-0.0558871163442376
5	20	-0.05265928146618393
5	21	-0.06816505211764934
5	22	-0.0808545376046439
5	23	-0.038147975069826145
5	24	-0.061849601663318765
5	25	-0.04029181849125507
5	26	-0.0425080509267417
5	27	-0.039864892887386816
5	28	-0.02782169410874502
5	29	-0.06997265238427275
5	30	0.02337442335233675
5	31	0.06442820265594662
5	32	0.04159353130138754
5	33	0.06791851604953791
5	34	0.07274041242649076
5	35	0.09475826216736039
5	36	0.05286718690228298
5	37	0.04352000402072065
5	38	0.07535652578913954
5	39	0.0539009392931078
5	40	0.037925119075040925
5	41	0.021965133286229843
5	42	0.03879903867346712
5	43	0.03697462967324583
5	44	-0.0158294122353837
5	45	0.022636053929389277
5	46	0.018472764154289223
5	47	-0.022960322001786437
5	48	-0.032889659096170476
5	49	-0.01091918836631578
5	50	0.024001425738696014
6	1	0.0020371881090923694
6	2	-0.016085893424580888
6	3	-0.013807332303031217
6	4	-0.030681394153539543
6	5	-0.028069953183518237
6	6	-0.04580845366375996
6	7	-0.013351999506269863
6	8	-0.01651911625437208
6	9	-0.007878386758982003
6	10	-0.037070775068109185
6	11	-0.0432773608780721
6	12	-0.05423366594932588
6	13	-0.05215977772142821
6	14	-0.06971453380342751
6	15	-0.05852128844797733
6	16	-0.0853295507041949
6	17	-0.07210103209725816
6	18	-0.06226465928539682
6	19	-0.057087966365351406
6	20	-0.06889927507626226
6	21	-0.07752496076742688
6	22	-0.08714193304808049
6	23	-0.06300691104936362
6	24	-0.06763989865932274
6	25	-0.08346162615179509
6	26	-0.08100092380094281
6	27	-0.08281419542679913
6	28	-0.09754287943070494
6	29	-0.08648611194143285
6	30	-0.05222201113893732
6	31	-0.03373836726842683
6	32	-0.03864324542725939
6	33	-0.04168287355980027
6	34	-0.008638114874983671
6	35	-0.04782880026797187
6	36	-0.06708777043095182
6	37	-0.07402738977988577
6	38	-0.07299671328065216
6	39	-0.07738469682669802
6	40	-0.08040467532408258
6	41	-0.08201594386291333
6	42	-0.06179310417419259
6	43	-0.06359846270659375
6	44	-0.10707198807630647
6	45	-0.09217622968174766
6	46	-0.16378918362471495
6	47	-0.10946556675498442
6	48	-0.08118877791462609
6	49	-0.0841322336068373
6	50	-0.07118393469114892
7	1	0.02896812661118986
7	2	0.011655278618739828
7	3	-0.0042648412130859015
7	4	0.007621502712254369
7	5	0.012080806657927212
7	6	0.0016269322855819723
7	7	-0.016288647434713255
7	8	0.0024569750340148744
7	9	-0.007289938414857033
7	10	0.0016944273810253208
7	11	-0.017489895768505903
7	12	-0.037189810786181
7	13	-0.0341339887560963
7	14	-0.034420392805742495
7	15	-0.024427595539108893
7	16	-0.06938967149232411
7	17	-0.014203317842877539
7	18	-0.051089430268454805
7	19	-0.03135208500703612
7	20	-0.047848720812389445
7	21	-0.04874536621363035
7	22	-0.031007152198718302
7	23	-0.050214281604631594
7	24	-0.07540092822823397
7	25	-0.03946344026466164
7	26	-0.04281463728803914
7	27	-0.05056667932655756
7	28	-0.06381589052003278
7	29	-0.05558879866376998
7	30	-0.04144962672266672
7	31	0.0038334120835017416
7	32	-0.00775248805713602
7	33	-0.02723667068559621
7	34	-0.017914070430308016
7	35	0.0024785352261460503
7	36	-0.01747124925544341
7	37	-0.017318310584521157
7	38	-0.08553596408300024
7	39	-0.08271905595060502
7	40	-0.07466857035494456
7	41	-0.023631113281844307
7	42	-0.022003572866267973
7	43	-0.10258707735609107
7	44	-0.053915793805877854
7	45	-0.03607464533371996
7	46	-0.027771748392858342
7	47	-0.07850580472501889
7	48	-0.07346830509992941
7	49	-0.10313095362293723
7	50	-0.04953007211995697
8	1	-0.017089304285114792
8	2	-0.02058313042067539
8	3	-0.039044651603717284
8	4	-0.0351443322579712
8	5	-0.018822960061896765
8	6	-0.04156613115353025
8	7	-0.03679158036791108
8	8	-0.025719715851138858
8	9	-0.02359143942555164
8	10	-0.038769558450098884
8	11	-0.04134234593853847
8	12	-0.048322272700218605
8	13	-0.061081500748542794
8	14	-0.08733424760229269
8	15	-0.06456653342000607
8	16	-0.06795751278588824
8	17	-0.045194632118452205
8	18	-0.08160273108624949
8	19	-0.057619844827428324
8	20	-0.08475426432622157
8	21	-0.06451643733240076
8	22	-0.09847875326595812
8	23	-0.09511144465445653
8	24	-0.08751344834642794
8	25	-0.0849557705552968
8	26	-0.0794439861292986
8	27	-0.09459706452388872
8	28	-0.09942510565544893
8	29	-0.09957572536252002
8	30	-0.06515516674941324
8	31	-0.034174938373922714
8	32	-0.05106480714124473
8	33	-0.07289138482720858
8	34	-0.03283058452264953
8	35	-0.06541102609954663
8	36	-0.09268713074838075
8	37	-0.10331931629545466
8	38	-0.09245044941812353
8	39	-0.1191823476868592
8	40	-0.12383781791628223
8	41	-0.11309886007469316
8	42	-0.18234132126744385
8	43	-0.1764512614208229
8	44	-0.13943646400198162
8	45	-0.1590699732048222
8	46	-0.16111785570894455
8	47	-0.15292432986388604
8	48	-0.17069710717882458
8	49	-0.19925597789819705
8	50	-0.20342535333272593
9	1	-0.021084945834168423
9	2	-0.06167122082405285
9	3	-0.0515762762581744
9	4	-0.04228016607083873
9	5	-0.044111877046695724
9	6	-0.06522276499729429
9	7	-0.05816887233761747
9	8	-0.07539642062476304
9	9	-0.06610866033811647
9	10	-0.055269258185404624
9	11	-0.05791330761675084
9	12	-0.0666618433209436
9	13	-0.06616446608506976
9	14	-0.07743085111384573
9	15	-0.07880742626026205
9	16	-0.06976822907148517
9	17	-0.07985667135615415
9	18	-0.08425639558428344
9	19	-0.0740302973934277
9	20	-0.08321007003831227
9	21	-0.09775258934624276
9	22	-0.08656027854883064
9	23	-0.08928396029819652
9	24	-0.08076257909642948
9	25	-0.0997822716337069
9	26	-0.09768045203954046
9	27	-0.11271179650258034
9	28	-0.14095857518849897
9	29	-0.10293673057326203
9	30	-0.057876092205987106
9	31	-0.03399958678751602
9	32	-0.08587094528338213
9	33	-0.07080354103008091
9	34	-0.0836631839448998
9	35	-0.04351910954731153
9	36	-0.032144674173700594
9	37	-0.04341219687680109
9	38	-0.03762935941013268
9	39	-0.05238555922819188
9	40	-0.04063614555132489
9	41	-0.0978479300910422
9	42	-0.10373157851061166
9	43	-0.11222473578087033
9	44	-0.1077451808677452
9	45	-0.11386056176080217
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114	18	-0.15548010804433687
114	19	-0.09899584170321418
114	20	-0.12049583748834891
114	21	-0.11977255693349065
114	22	-0.11467501640342448
114	23	-0.08910497441211618
114	24	-0.08919638657491902
114	25	-0.11817989936476891
114	26	-0.08094677456776367
114	27	-0.09917707269909926
114	28	-0.0867882603667951
114	29	-0.06430523015346523
114	30	-0.01181621988088466
114	31	0.03663293987796834
114	32	0.01891158911257662
114	33	0.026284869473215622
114	34	0.02519002696192274
114	35	0.06698258687863046
114	36	-0.00642921859052592
114	37	-0.034393412787622424
114	38	0.03176476407742257
114	39	0.012647691669130268
114	40	0.04767002016990318
114	41	0.04878315249102627
114	42	0.06497261122462561
114	43	0.06469416633100167
114	44	0.06911180270996198
114	45	0.09421026543853372
114	46	0.1273023384858618
114	47	0.1382634673946157
114	48	0.16865646880462037
114	49	0.16119583971985207
114	50	0.1949331269127157
115	1	-0.04729810916686006
115	2	-0.0839572719672006
115	3	-0.08408965745299213
115	4	-0.03125660383673079
115	5	-0.08499265636898912
115	6	-0.07013590364050515
115	7	-0.07955610179002548
115	8	-0.08245597619210798
115	9	-0.06013238144709021
115	10	-0.06012034653928566
115	11	-0.08976976393235958
115	12	-0.0793921713232919
115	13	-0.1025389083967454
115	14	-0.08181527599705163
115	15	-0.10940747598187528
115	16	-0.10940664001184075
115	17	-0.06717431126050855
115	18	-0.08012930713655919
115	19	-0.09181111177484524
115	20	-0.092266221291041
115	21	-0.10722355718330334
115	22	-0.08385033475853731
115	23	-0.06043661508203968
115	24	-0.09354299920860853
115	25	-0.10037370627269837
115	26	-0.04540218782087635
115	27	-0.037233597283730546
115	28	-0.06246726542535441
115	29	-0.0451506150802814
115	30	-0.012960998706944338
115	31	0.02616695464709551
115	32	0.05071933614486568
115	33	0.08964776034269306
115	34	0.029609904328218306
115	35	0.10674119809554838
115	36	0.07175272219412676
115	37	0.05964641096666412
115	38	0.04419041203770746
115	39	0.0756847589041385
115	40	0.07157797190863846
115	41	0.06995910126710214
115	42	0.08038426673200405
115	43	0.06636447230773967
115	44	0.014070073777340042
115	45	-0.0073301828075713615
115	46	0.05769258304045621
115	47	0.0986972820662686
115	48	0.11031740734878781
115	49	0.14679690270281753
115	50	0.13015272027756453
116	1	-0.08992383914554836
116	2	-0.09434581060065028
116	3	-0.11951511262619618
116	4	-0.07958468943028407
116	5	-0.12816672597552525
116	6	-0.11669419295308359
116	7	-0.1245392766955149
116	8	-0.11118959432824482
116	9	-0.09822139220665349
116	10	-0.10216424013814418
116	11	-0.11548400788156954
116	12	-0.11391099043277109
116	13	-0.13005094526207728
116	14	-0.1458519118178998
116	15	-0.13898572259540032
116	16	-0.1343257940414091
116	17	-0.11695636713492519
116	18	-0.13331377811009304
116	19	-0.12359670795807176
116	20	-0.123235640547108
116	21	-0.16597358621136493
116	22	-0.1026698072018064
116	23	-0.09499820830729533
116	24	-0.12466413385178754
116	25	-0.11944114735742772
116	26	-0.059607989014537566
116	27	-0.08684593837860177
116	28	-0.07524161826395215
116	29	-0.08909514506878935
116	30	0.023074274962674224
116	31	0.06740199230579691
116	32	0.08109575545358183
116	33	0.08061390193565643
116	34	0.05788285682812244
116	35	0.12148065580711886
116	36	0.0619420103964714
116	37	0.07966538436131998
116	38	0.054735201900328434
116	39	0.095049693879389
116	40	0.10764869386749609
116	41	0.1022537631467415
116	42	0.10564554593446474
116	43	0.086447536702984
116	44	0.11572560797443998
116	45	0.04327094281831734
116	46	0.1460327905236838
116	47	0.10348726920351936
116	48	0.13522495741433005
116	49	0.16446833283281848
116	50	0.21428913136335126
117	1	-0.05899799365413827
117	2	-0.07876606921509932
117	3	-0.13817108770990671
117	4	-0.08755143901113627
117	5	-0.07351634142032992
117	6	-0.1197811838526448
117	7	-0.09967795217496445
117	8	-0.10316114665459253
117	9	-0.11940101577967965
117	10	-0.16334465273119747
117	11	-0.11292429350758226
117	12	-0.13341681535266048
117	13	-0.12496531534593913
117	14	-0.12400169496656588
117	15	-0.13130532343576107
117	16	-0.14407130468088525
117	17	-0.1379359619513565
117	18	-0.13434300820482292
117	19	-0.15187837529992976
117	20	-0.13699490044099605
117	21	-0.09461116134233549
117	22	-0.10371826799448769
117	23	-0.11735563838551855
117	24	-0.12078871526117752
117	25	-0.10522906923163333
117	26	-0.14094873871744085
117	27	-0.15103290312546136
117	28	-0.0990348492666584
117	29	-0.09258638741036407
117	30	-0.046970038284200655
117	31	-0.030247307503870502
117	32	-0.0371063313873492
117	33	-0.04635873912319255
117	34	0.015460581806962637
117	35	0.018196349953548463
117	36	-0.028905973890729797
117	37	-0.0018066474455125103
117	38	-0.03198672243083678
117	39	-0.034416063010645814
117	40	-0.04087235341538076
117	41	0.005423728180225851
117	42	0.0021396874060499727
117	43	0.04608026776768526
117	44	0.01885247045766647
117	45	-0.005794096758116041
117	46	0.008576602509236864
117	47	0.026646907228496275
117	48	0.007821983100953389
117	49	-0.03976925613170579
117	50	0.031156817105710388
118	1	-0.9640117376161399
118	2	-1.1777813884881425
118	3	-1.2104842183388342
118	4	-1.085738490932222
118	5	-1.0180899423829004
118	6	-0.9475965801474047
118	7	-1.0638870098862157
118	8	-0.9895871856128124
118	9	-1.0657540927115718
118	10	-1.1845397005863774
118	11	-1.0145577055586443
118	12	-0.9926846455307725
118	13	-1.0063582174386596
118	14	-0.955580865613463
118	15	-0.9642426697582422
118	16	-0.9814056683574819
118	17	-0.9762072831957838
118	18	-1.024725472931678
118	19	-1.0011197770060818
118	20	-0.9404598077059916
118	21	-0.9264716844961711
118	22	-0.9457147062930531
118	23	-0.9767773954390648
118	24	-1.0165692599770182
118	25	-0.9985410234503931
118	26	-1.2031374303534932
118	27	-1.0838208547559347
118	28	-1.4263339830996173
118	29	-1.5977122300651203
118	30	-1.6250020207952574
118	31	-1.6873830001636776
118	32	-1.787320317537997
118	33	-1.7801880379690118
118	34	-1.8777641730834276
118	35	-1.891511199891461
118	36	-2.0480109649453837
118	37	-2.084698294625511
118	38	-2.1548870741524766
118	39	-2.1613608019175032
118	40	-2.224286132630688
118	41	-2.313147186974298
118	42	-2.3170523146963546
118	43	-2.391131576033459
118	44	-2.3905311171837553
118	45	-2.493573773231354
118	46	-2.470979656538418
118	47	-2.4898199585882566
118	48	-2.538779053425685
118	49	-2.5946362151802376
118	50	-2.590835409385349
120	1	-1.881651457285713
120	2	-1.7333290920526068
120	3	-1.744916580261851
120	4	-1.6766716917077034
120	5	-1.6532662929047817
120	6	-1.364292611967386
120	7	-1.2387911994275527
120	8	-1.2193376819324016
120	9	-1.2357394526938208
120	10	-1.1399733143600557
120	11	-1.2122890870408938
120	12	-1.0145961162008632
120	13	-1.1405350623756618
120	14	-0.887890595640549
120	15	-1.1394303192887634
120	16	-1.1153055611586424
120	17	-1.1201453359369324
120	18	-1.1315384055912645
120	19	-1.1180191382310554
120	20	-1.0621532318905338
120	21	-1.0072789208833015
120	22	-0.9261387322797106
120	23	-0.9422465365418589
120	24	-0.9189958803329219
120	25	-0.9311303676936973
120	26	-1.1036961027942453
120	27	-1.0953158578321336
120	28	-1.31392785747191
120	29	-1.4000680096132925
120	30	-1.0487738549072034
120	31	-1.1060530087923084
120	32	-1.0953838331845915
120	33	-1.1330522176504445
120	34	-1.1387267686782039
120	35	-1.0579875228991185
120	36	-0.9449714722903622
120	37	-0.927051092741884
120	38	-0.8944211063526382
120	39	-0.8811228484734315
120	40	-0.8519853722427868
120	41	-0.8004860404309184
120	42	-0.7516797123178378
120	43	-0.7500072734954557
120	44	-0.7385987756561079
120	45	-0.7762689447474997
120	46	-0.639588426267153
120	47	-0.646504850338566
120	48	-0.6172977872245369
120	49	-0.5930768942282185
120	50	-0.6357116922201484
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	19009.0
3	1518.0
4	1753.0
5	1088.0
6	1659.0
7	2518.0
8	3032.0
9	3445.0
10	5060.0
11	6690.0
12	7963.0
13	10210.0
14	12639.0
15	14938.0
16	17373.0
17	20164.0
18	23477.0
19	29812.0
20	35203.0
21	33049.0
22	41896.0
23	58676.0
24	75212.0
25	74650.0
26	110616.0
27	141875.0
28	165580.0
29	174211.0
30	215134.0
31	265584.0
32	329854.0
33	397897.0
34	559081.0
35	806769.0
36	1202886.0
37	2097153.0
38	3869544.0
39	1.7660974E7
40	368034.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.95615369980899	19.37058489103617	11.866951537072357	23.806309872082483
2	17.085582992387017	22.053232729488087	36.45819512394866	24.402989154176232
3	18.150864361352014	26.18391883536464	32.017031148713606	23.648185654569737
4	11.853674228519875	16.12764505417462	38.21438404421924	33.80429667308627
5	12.27077615204876	39.835667791085896	36.02766575021874	11.865890306646595
6	30.7432741640698	37.81270540873615	17.847514947052655	13.596505480141394
7	27.115591764576358	33.534376818084915	22.799821493810796	16.55020992352793
8	28.53924167295025	34.02370645889074	20.637176470523023	16.799875397635976
9	27.17589407080787	12.86460516175547	20.018668183364184	39.940832584072474
10	16.63499066348334	27.888356448120373	35.08287158840923	20.39378129998705
11	35.05459286572481	21.868161774940724	23.55347734061252	19.52376801872195
12	20.571314033223466	26.7420340989501	32.598549599105894	20.088102268720544
13	29.381423120570037	20.67032247305207	26.489268115617193	23.4589862907607
14	20.665964438856676	20.484787308185005	29.66757414010408	29.18167411285424
15	23.767218478785555	29.062739271839693	24.490250301511534	22.679791947863222
16	22.744178612056874	27.479567297782538	26.619832464417065	23.156421625743526
17	20.765145182907293	27.996597266949646	28.262742373366116	22.97551517677694
18	21.029643829511745	27.562157869473253	29.229212764447915	22.17898553656709
19	21.880698917828745	27.10608238153474	29.91018985301369	21.103028847622824
20	23.082387008263566	26.060396672568142	29.586766901915063	21.27044941725323
21	23.165196586488307	26.045531549569382	29.16312648560293	21.62614537833938
22	22.118284530856233	25.877123666945582	29.472470699841402	22.53212110235678
23	21.992728966334248	26.848691245811825	29.302923199143954	21.85565658870998
24	22.381036159164854	26.276091485253676	29.44998616917072	21.892886186410752
25	21.935372619162326	26.842631302442577	29.57946706904815	21.64252900934694
26	21.72091028030995	26.150371930558176	29.472488965270976	22.6562288238609
27	21.37975415837012	26.359206819916924	29.44900512841272	22.812033893300235
28	22.382853335863935	26.23112247447904	29.65163863260734	21.734385557049684
29	21.548972172923364	27.026389253397294	29.638723524780364	21.785915048898982
30	21.55647231640094	26.580743204078694	30.009218731236775	21.853565748283597
31	21.85978137016532	26.796706274726155	29.492813992753003	21.850698362355526
32	21.59507333188104	26.428639699723373	30.280875836399584	21.695411131996
33	21.577550469939936	26.518808584785607	29.451064211202237	22.45257673407222
34	22.71634206267023	25.960052706424424	29.55156349752201	21.77204173338333
35	21.67217676435931	26.357856400247652	30.33135065053225	21.638616184860783
36	21.982239459998056	27.406273782414058	28.97378173812173	21.637705019466157
37	22.02142200447642	26.29381992241217	30.003322221711944	21.681435851399467
38	22.67609690758917	26.901283830937867	28.931362048758412	21.491257212714547
39	21.710097714220506	26.826523352939073	29.046569214904338	22.41680971793608
40	21.739830388296014	26.471109164657967	29.268757916160222	22.52030253088579
41	22.41078491135231	26.55444382099832	29.003750235930685	22.03102103171869
42	21.468796150722177	26.926857735158006	29.775883656540632	21.828462457579185
43	21.79627901367137	26.591735246476567	29.006768913149372	22.605216826702694
44	21.792491982736376	26.87028244149299	29.598959429911524	21.738266145859107
45	21.62797909963848	26.862238433809388	29.625748865870715	21.884033600681416
46	21.890993241030042	26.859536577395332	28.547914980985816	22.701555200588814
47	21.7508871579229	27.097673938311946	29.411429375696517	21.740009528068637
48	22.67291795133396	27.23855616294949	28.05820856977453	22.030317315942025
49	22.05550944367436	26.992994549868044	28.329986383944878	22.62150962251272
50	21.965431732983483	27.132438428523216	28.957736861033627	21.94439297745968
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	29939.0
1	20502.5
2	11066.0
3	28696.0
4	46326.0
5	47464.5
6	48603.0
7	36369.5
8	24136.0
9	33330.5
10	42525.0
11	64686.5
12	86848.0
13	143727.5
14	200607.0
15	198087.0
16	195567.0
17	249141.5
18	302716.0
19	286318.0
20	269920.0
21	300512.0
22	331104.0
23	375508.5
24	419913.0
25	474350.5
26	528788.0
27	606379.0
28	683970.0
29	758754.5
30	833539.0
31	923137.5
32	1012736.0
33	1101016.5
34	1189297.0
35	1280000.0
36	1370703.0
37	1438895.0
38	1507087.0
39	1556102.0
40	1605117.0
41	1642430.0
42	1679743.0
43	1717032.0
44	1754321.0
45	1748560.0
46	1742799.0
47	1716693.0
48	1690587.0
49	1654120.0
50	1617653.0
51	1575556.5
52	1533460.0
53	1494585.5
54	1455711.0
55	1390047.5
56	1324384.0
57	1311686.0
58	1298988.0
59	1214666.0
60	1130344.0
61	988712.5
62	847081.0
63	740675.5
64	634270.0
65	554442.0
66	474614.0
67	403241.0
68	331868.0
69	285210.0
70	238552.0
71	198444.0
72	158336.0
73	129316.5
74	100297.0
75	77158.5
76	54020.0
77	42120.0
78	30220.0
79	22757.0
80	15294.0
81	11139.5
82	6985.0
83	5153.5
84	3322.0
85	2411.0
86	1500.0
87	1124.5
88	749.0
89	455.5
90	162.0
91	109.0
92	56.0
93	48.0
94	40.0
95	44.5
96	49.0
97	55.0
98	61.0
99	157.0
100	253.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1775500545169985
2	0.0
3	2.0092685479563557E-4
4	0.019621546647628964
5	2.251766476157985E-4
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	6.235661010899034E-5
18	1.7321280585830653E-4
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0015727722771934232
24	0.010981691891416633
25	0.014075272604045988
26	0.05524795655656545
27	0.044529548130053435
28	0.048707441007355794
29	0.010915871025190477
30	0.011265760893024257
31	0.035148342564767565
32	0.03706754045367759
33	0.05228255332027124
34	0.05754822261836376
35	0.08952330657980714
36	0.06042009093949448
37	0.052140518819467435
38	0.10511245910705475
39	0.1255307846616319
40	0.11090123107883934
41	0.09806269790862164
42	0.11566804749605993
43	0.09927518754962979
44	0.10830303899096473
45	0.10318979696202753
46	0.06609454245941261
47	0.09430744427761356
48	0.09032701399898968
49	0.08500938085913967
50	0.10035603545818564
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2.8866226E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.807471228704138
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.40660822333368	20.80418809230809
2	14.134722212125464	8.99179540175812
3	6.598871484423029	6.296802446481049
4	3.578560879049611	4.552998888021468
5	2.3144421239999877	3.680827563481525
6	1.4753960294766582	2.815717005511387
7	1.0728173195848931	2.3886524218446383
8	0.8216696526834013	2.090818706978128
9	0.6042332993228615	1.7297219955273204
>10	3.497712941993191	20.210095924323976
>50	0.28363656729974823	6.287037580313773
>100	0.19041883871274934	11.621290776163576
>500	0.014835288238409565	3.2084189971686827
>1k	0.005888391950887587	3.049911556463359
>5k	6.591099011748194E-5	0.11940398875387996
>10k+	1.20836815174986E-4	2.1523186549012734
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	129480	0.4485518820506706	Illumina PCR Primer Index 1 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	105498	0.3654720918487924	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	99771	0.345632297065782	No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT	74969	0.2597118168478276	Illumina Paired End PCR Primer 2 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	62657	0.21705989553327823	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34368	0.11905955423476558	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33968	0.1176738517878991	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28910	0.10015164434727282	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	3.4642561171661302E-6	0.0	0.0	0.0	0.0
4	3.4642561171661302E-6	0.0	0.0	0.0	0.0
5	3.4642561171661302E-6	1.3857024468664521E-5	0.0	0.0	3.4642561171661302E-6
6	6.9285122343322605E-6	1.3857024468664521E-5	0.0	0.0	6.9285122343322605E-6
7	1.732128058583065E-5	1.3857024468664521E-5	0.0	0.0	1.039276835149839E-5
8	4.849958564032582E-5	1.732128058583065E-5	0.0	0.0	1.3857024468664521E-5
9	5.1963841757491954E-5	1.732128058583065E-5	0.0	1.732128058583065E-5	1.3857024468664521E-5
10	5.5428097874658084E-5	1.732128058583065E-5	0.0	7.621363457765487E-5	1.732128058583065E-5
11	6.92851223433226E-5	1.732128058583065E-5	0.0	9.007065904631939E-5	1.732128058583065E-5
12	6.92851223433226E-5	1.732128058583065E-5	0.0	1.8706983032697105E-4	1.732128058583065E-5
13	7.9677890694821E-5	2.7714048937329042E-5	0.0	2.1824813538146623E-4	1.732128058583065E-5
14	3.18711562779284E-4	2.7714048937329042E-5	0.0	3.256400750136163E-4	1.732128058583065E-5
15	3.429613555994469E-4	2.7714048937329042E-5	0.0	4.572818074659292E-4	1.732128058583065E-5
16	4.642103197002615E-4	2.7714048937329042E-5	0.0	5.612094909809132E-4	3.117830505449517E-5
17	4.642103197002615E-4	2.7714048937329042E-5	0.0	7.586720896593825E-4	3.117830505449517E-5
18	4.676745758174276E-4	2.7714048937329042E-5	0.0	8.937780782288617E-4	3.46425611716613E-5
19	4.676745758174276E-4	2.7714048937329042E-5	0.0	0.0011639900553678199	3.46425611716613E-5
20	4.676745758174276E-4	2.7714048937329042E-5	0.0	0.0012540607144141392	4.157107340599356E-5
21	5.092456492234212E-4	3.117830505449517E-5	0.0	0.001434202032506778	5.5428097874658084E-5
22	5.127099053405873E-4	3.117830505449517E-5	0.0	0.001621271862833749	5.5428097874658084E-5
23	5.127099053405873E-4	3.117830505449517E-5	0.0	0.001908805120558538	5.8892353991824214E-5
24	5.161741614577534E-4	3.117830505449517E-5	0.0	0.0021131962314713394	5.8892353991824214E-5
25	5.265669298092518E-4	3.810681728882743E-5	0.0	0.0023487656474386366	6.235661010899034E-5
26	5.646737470980793E-4	3.810681728882743E-5	0.0	0.002556621014468604	6.582086622615648E-5
27	5.889235399182422E-4	3.810681728882743E-5	0.0	0.0026397631612805916	7.621363457765487E-5
28	6.37423125558568E-4	3.810681728882743E-5	0.0	0.0029411534434740445	7.9677890694821E-5
29	6.37423125558568E-4	3.810681728882743E-5	0.0	0.0033326143847138175	7.9677890694821E-5
30	6.478158939100663E-4	3.810681728882743E-5	0.0	0.0038349315217029063	7.9677890694821E-5
31	6.789941989645616E-4	3.810681728882743E-5	0.0	0.004319927378106165	7.9677890694821E-5
32	6.824584550817277E-4	3.810681728882743E-5	0.0	0.005050885418828218	7.9677890694821E-5
33	6.824584550817277E-4	3.810681728882743E-5	0.0	0.005903092423651086	8.660640292915326E-5
34	6.824584550817277E-4	3.810681728882743E-5	0.0	0.007077475247370404	9.353491516348552E-5
35	6.859227111988938E-4	5.1963841757491954E-5	0.0	0.008833853098773633	9.353491516348552E-5
36	6.859227111988938E-4	5.1963841757491954E-5	0.0	0.01167454311484986	9.353491516348552E-5
37	6.859227111988938E-4	5.1963841757491954E-5	0.0	0.0167843208876699	9.353491516348552E-5
38	6.859227111988938E-4	5.1963841757491954E-5	0.0	0.02356040585284685	9.353491516348552E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	27560	0.0	36.201824	35
GACCGAT	28090	0.0	35.268734	24
TCGTATG	28345	0.0	35.143616	32
GCCGTCT	28565	0.0	35.03295	38
CGTATGC	28590	0.0	35.01673	33
CCGTCTT	28630	0.0	34.960434	39
TATCGTA	17190	0.0	34.959503	30
ATATCGT	17220	0.0	34.872803	29
ATCGTAT	17980	0.0	34.023212	31
ACCGATA	17965	0.0	33.999268	25
ATGCCGT	29430	0.0	33.96892	36
CGTCTTC	29525	0.0	33.896553	40
GCGGGCT	30985	0.0	33.887432	8
TGAGCGG	31150	0.0	33.863262	5
AGACCGA	30115	0.0	33.861526	23
GATATCG	18090	0.0	33.66563	28
CGATATC	18100	0.0	33.658543	27
TGCCGTC	29905	0.0	33.488266	37
CCGATAT	18280	0.0	33.26901	26
CGGGCTG	32095	0.0	32.729145	9
>>END_MODULE