SRR522086_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR522086_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27637598
Sequences flagged as poor quality	0
Sequence length	50
%GC	44

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	200058	0.7238617480433719	Illumina PCR Primer Index 1 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	122505	0.4432548733070074	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	115987	0.41967105824464196	No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT	106062	0.38375983325323715	Illumina Paired End PCR Primer 2 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	74659	0.27013563190259876	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	53834	0.19478537896093576	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40385	0.1461234076854291	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39303	0.1422084509659631	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33520	0.12128405659565639	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	40170	36.654984	35
CCGTCTT	40280	36.37462	39
GCCGTCT	40335	36.336006	38
GGTATCA	47905	36.06158	1
CGTCTTC	40680	35.943653	40
GACCGAT	40860	35.492657	24
TCGTATG	41475	35.325665	32
ATGCCGT	41480	35.263798	36
TGCCGTC	41720	35.09788	37
CGTATGC	41965	35.067467	33
TGAGCGG	43865	34.802547	5
ATATCGT	26135	34.752464	29
ACCGATA	26365	34.629444	25
GCGGGCT	44125	34.57244	8
TATCGTA	26315	34.55743	30
AGACCGA	42910	34.545555	23
ATCGTAT	26680	34.505257	31
CCGATAT	26585	34.284943	26
CGATATC	26640	34.262283	27
GATATCG	26750	34.154102	28