##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522086_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27637598
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.079350781497006	40.0	39.0	40.0	38.0	40.0
2	38.961020237721094	40.0	39.0	40.0	38.0	40.0
3	38.91217521870026	40.0	39.0	40.0	37.0	40.0
4	38.96250506284953	40.0	39.0	40.0	38.0	40.0
5	38.95976922451799	40.0	39.0	40.0	38.0	40.0
6	39.01902053861555	40.0	39.0	40.0	38.0	40.0
7	38.96713104373253	40.0	39.0	40.0	38.0	40.0
8	38.95694940638474	40.0	39.0	40.0	38.0	40.0
9	38.95910805996961	40.0	39.0	40.0	38.0	40.0
10	38.90947440512016	40.0	39.0	40.0	37.0	40.0
11	39.00054418622052	40.0	39.0	40.0	38.0	40.0
12	38.9303418480868	40.0	39.0	40.0	38.0	40.0
13	38.93568127736716	40.0	39.0	40.0	38.0	40.0
14	38.90225782283974	40.0	39.0	40.0	37.0	40.0
15	38.88180278908464	40.0	39.0	40.0	37.0	40.0
16	38.851356148967795	40.0	39.0	40.0	37.0	40.0
17	38.81627741310949	40.0	39.0	40.0	37.0	40.0
18	38.796738016089535	40.0	39.0	40.0	36.0	40.0
19	38.687049142259035	40.0	39.0	40.0	36.0	40.0
20	38.73191816452356	40.0	39.0	40.0	36.0	40.0
21	38.686421410427926	40.0	39.0	40.0	36.0	40.0
22	38.61191848148309	40.0	39.0	40.0	36.0	40.0
23	38.58376281469902	40.0	39.0	40.0	36.0	40.0
24	38.524780084000064	40.0	39.0	40.0	36.0	40.0
25	38.48638213783991	40.0	38.0	40.0	35.0	40.0
26	38.35613749791136	40.0	38.0	40.0	35.0	40.0
27	38.27572602365806	40.0	38.0	40.0	35.0	40.0
28	38.215189720901215	40.0	38.0	40.0	35.0	40.0
29	38.278500360270094	40.0	38.0	40.0	35.0	40.0
30	38.171530680777686	40.0	38.0	40.0	35.0	40.0
31	37.92772110658821	40.0	38.0	40.0	35.0	40.0
32	37.97651015113542	40.0	38.0	40.0	35.0	40.0
33	37.91577459083094	40.0	38.0	40.0	35.0	40.0
34	37.86147030577693	40.0	38.0	40.0	34.0	40.0
35	37.92780052738302	40.0	38.0	40.0	35.0	40.0
36	38.001807610053525	40.0	39.0	40.0	35.0	40.0
37	37.93079029516241	40.0	38.0	40.0	35.0	40.0
38	37.866979033416726	40.0	38.0	40.0	35.0	40.0
39	37.7651536866554	40.0	38.0	40.0	35.0	40.0
40	37.67916585949329	40.0	38.0	40.0	35.0	40.0
41	37.56683999817929	40.0	38.0	40.0	35.0	40.0
42	37.44573490069578	40.0	38.0	40.0	34.0	40.0
43	37.35955045731543	40.0	38.0	40.0	33.0	40.0
44	37.22878956412927	40.0	38.0	40.0	33.0	40.0
45	37.061944891158774	40.0	38.0	40.0	33.0	40.0
46	36.712724564558755	40.0	38.0	40.0	33.0	40.0
47	36.615223037834184	40.0	38.0	40.0	32.0	40.0
48	36.479800704822466	40.0	38.0	40.0	32.0	40.0
49	36.34788783019422	39.0	37.0	40.0	31.0	40.0
50	36.273320966604985	39.0	37.0	40.0	31.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1	1	-0.4365126216278128
1	2	-0.587688202940825
1	3	-0.7612124807140717
1	4	-0.5762908449002921
1	5	-0.5065717284357021
1	6	-0.8083735704159309
1	7	-2.323113819232674
1	8	-1.202776762195974
1	9	-3.341486971926649
1	10	-2.799850894969481
1	11	-0.9390647617875985
1	12	-0.9856552316102309
1	13	-1.0188004248574316
1	14	-0.9792892553619055
1	15	-1.1055121616383445
1	16	-1.4412958874041166
1	17	-1.0591362045625061
1	18	-1.047082945545867
1	19	-9.771113334802127
1	20	-1.0590495901562988
1	21	-1.1154394600670514
1	22	-1.1187247261568274
1	23	-1.1274206916029712
1	24	-1.113279512847555
1	25	-1.1900061860945428
1	26	-0.48940081148592895
1	27	-0.45090284024824
1	28	-0.46896042705169094
1	29	-0.3467874980681316
1	30	-0.29812178511353693
1	31	-0.2896791822959841
1	32	-0.26903253431074603
1	33	-0.31113515666060465
1	34	-0.2512996575547888
1	35	-0.33246848350888314
1	36	-0.41839378122953974
1	37	-0.49739434298891183
1	38	-0.40115825790140036
1	39	-0.4742594186489981
1	40	-0.5246093091992989
1	41	-0.40546609677822687
1	42	-0.3990330183215818
1	43	-0.42025051287773607
1	44	-0.4307019356321291
1	45	-0.36059040167263845
1	46	-0.39065632751196944
1	47	-0.39285710580681155
1	48	-0.37967026309424057
1	49	-0.4505923370482918
1	50	-0.47217563157870046
2	1	-0.11103611362763388
2	2	-0.16511989516506986
2	3	-0.16082766499098256
2	4	-0.1289879963634064
2	5	-0.11726182076332492
2	6	-0.09000932530554451
2	7	-0.07094068756876482
2	8	-0.0746016035408914
2	9	-0.08091397800985334
2	10	-0.10749847805994506
2	11	-0.09483697397038071
2	12	-0.14264059098549353
2	13	-0.13521452428621217
2	14	-0.111229661898264
2	15	-0.08448516260507688
2	16	-0.08916209297510136
2	17	-0.12310844946330946
2	18	-0.10861439838288334
2	19	-0.034729741964234506
2	20	-0.09728203003902536
2	21	-0.11080257519120806
2	22	-0.10313459930854663
2	23	-0.102376655352856
2	24	-0.14252544070568263
2	25	-0.13235826003212736
2	26	-0.1230956767909035
2	27	-0.15090120716259747
2	28	-0.17435031896927455
2	29	-0.0824101365003429
2	30	-0.06156268948641497
2	31	-0.02795079526633515
2	32	-0.0146833819310217
2	33	-0.027650456477424257
2	34	0.007017946845408574
2	35	-0.03961867819743503
2	36	-0.041969419481013404
2	37	-0.02860842360426119
2	38	-0.002885139329137587
2	39	-0.03245533001241796
2	40	-0.06378614705102592
2	41	-0.02278074468835456
2	42	-0.02310732879835342
2	43	-0.08107279677819434
2	44	-0.07495025743444472
2	45	-0.10704098199405365
2	46	-0.03226679365709373
2	47	-0.04350381341121334
2	48	-0.04404264812409764
2	49	-0.07763716799885145
2	50	-0.060092320703290625
3	1	0.005180299681462941
3	2	-0.0447399455240145
3	3	-0.03611663304248225
3	4	-0.021169486011636707
3	5	-0.025941559483193544
3	6	-0.009021330962610818
3	7	1.0264469555210098E-4
3	8	-0.0025511027037836698
3	9	0.029611602068428056
3	10	0.014803462408487178
3	11	-0.0077375598882696295
3	12	-0.01757440655698872
3	13	-0.038707516006361686
3	14	-0.013656031206487285
3	15	-0.03316675538496838
3	16	-0.03845233181250052
3	17	-0.0149288682864821
3	18	-0.045969774488590076
3	19	0.04191256189852055
3	20	-0.04154400983587436
3	21	-0.02563854767544882
3	22	-0.03818095789954157
3	23	-0.03334755380460308
3	24	-0.030297021155590187
3	25	-0.05305480215755409
3	26	-0.037751625155848956
3	27	-0.05354071311946029
3	28	-0.062129336542462
3	29	-0.013992813489686284
3	30	0.005610283857436116
3	31	0.06091410501274197
3	32	0.006470772387224599
3	33	0.012209476674868824
3	34	0.029026244201219242
3	35	-0.002306272584789326
3	36	-9.112062679648147E-4
3	37	-0.0038957704750615108
3	38	0.0048685542855650965
3	39	-0.0010677702885857343
3	40	-0.014377660973785567
3	41	-0.020731425305370976
3	42	-0.017112601852510068
3	43	-0.05174271987029044
3	44	-0.03007377824333446
3	45	-0.09859904022125221
3	46	-0.1035651871771961
3	47	-0.09364427724107571
3	48	-0.09996130369724199
3	49	-0.09395493559184587
3	50	-0.035120465634115305
4	1	-0.0023723659897640914
4	2	-0.03657554231329385
4	3	-0.02977988873477244
4	4	-0.021588422778826555
4	5	-0.026195541715843262
4	6	-0.02970411207594026
4	7	-0.033092958449955745
4	8	-0.035670469303262564
4	9	0.0046472557872760945
4	10	-0.021979774635042304
4	11	-0.048682441958924016
4	12	-0.050687957013650475
4	13	-0.056891442249145996
4	14	-0.05445179900758035
4	15	-0.04533900445021288
4	16	-0.032353325250547016
4	17	-0.07432291452296624
4	18	-0.05508070695977807
4	19	0.02266938227727877
4	20	-0.05871930495325017
4	21	-0.060760972050942996
4	22	-0.06832309333572795
4	23	-0.03872845575315864
4	24	-0.04718423399496885
4	25	-0.06332945756449959
4	26	-0.08210957541398756
4	27	-0.08077148084765184
4	28	-0.07600021919844835
4	29	-0.050886846599865976
4	30	-0.06446578333704878
4	31	-0.04639083645010089
4	32	-0.07025668226318516
4	33	-0.0782036601038314
4	34	-0.0777917523364664
4	35	-0.07008479615201679
4	36	-0.06092184652320043
4	37	-0.060903462037494194
4	38	-0.06713014506205184
4	39	-0.07287386515871219
4	40	-0.0948481408639168
4	41	-0.04147724904473904
4	42	-0.0528709182086331
4	43	-0.08852962689993404
4	44	-0.06691075208439656
4	45	-0.07588280326146446
4	46	-0.03708141842727741
4	47	-0.05669012702469445
4	48	-0.05639302423583814
4	49	-0.09049105758071363
4	50	-0.06315517115737634
5	1	0.006872333212676551
5	2	-0.042368040547223984
5	3	-0.023140337994462357
5	4	-0.03946639754633452
5	5	-0.039688548734424955
5	6	-0.029493569949345044
5	7	-0.008503470788461698
5	8	-0.012065485559197953
5	9	-0.0034233797122240617
5	10	-0.015002259708232657
5	11	-0.03113792010033478
5	12	-0.027008963370093397
5	13	-0.01427989662315099
5	14	-0.05324298302378594
5	15	-0.020408257498544913
5	16	-0.036538772170516154
5	17	-0.029619570560733166
5	18	-0.05793173935444429
5	19	0.03143965634370005
5	20	-0.01857699478635766
5	21	-0.021313973307172773
5	22	-0.011555513562271358
5	23	-0.010491515524954309
5	24	-0.02322013740893425
5	25	-0.02740195177570115
5	26	0.010058661828423965
5	27	-0.034974852968247205
5	28	0.0012476713096631897
5	29	0.03933724829258978
5	30	0.05384306373046144
5	31	0.08289854637764904
5	32	0.10177893410997996
5	33	0.07358781641036671
5	34	0.11790113880405073
5	35	0.08686067095881356
5	36	0.08991638278253333
5	37	0.11930201715265554
5	38	0.11565770494480176
5	39	0.09708804105208202
5	40	0.12726334122478278
5	41	0.08603428205473307
5	42	0.11390322838420275
5	43	0.09533697827495757
5	44	0.07567239461858577
5	45	0.049018668646787944
5	46	0.08671881320262287
5	47	0.09528238269309242
5	48	0.12877026734404495
5	49	0.11490636529628517
5	50	0.12736699697183695
6	1	0.02335597040016779
6	2	-0.015493739562657538
6	3	-0.009030570561328943
6	4	0.0077546866153213045
6	5	-0.002907424579667861
6	6	0.0195087168895256
6	7	0.013886046694253196
6	8	0.013197861546281331
6	9	0.03100092636021401
6	10	0.017356839877201935
6	11	-0.02738984602917327
6	12	-0.014259931476310328
6	13	-0.035758137331079354
6	14	-0.014931342260510405
6	15	-0.018866639517376882
6	16	-0.021319592528499243
6	17	-0.006868926969850975
6	18	-0.011348234845307559
6	19	0.07693901357589539
6	20	-0.01649290052625929
6	21	-0.028789498992324525
6	22	0.0014835602344973609
6	23	-0.011625229961516936
6	24	-0.04101643244312214
6	25	-0.011813404005607708
6	26	0.009520811373711524
6	27	0.0114298140887783
6	28	-0.02241100068598456
6	29	0.028098308843013342
6	30	0.010682433006287795
6	31	0.03143829998969494
6	32	0.04909942127012101
6	33	0.03652811803726763
6	34	0.027639015702860092
6	35	0.015878848472617335
6	36	0.01141335156968637
6	37	0.03701362524301999
6	38	0.05804154307789133
6	39	0.012347532421060237
6	40	0.02352348117034353
6	41	0.04029677661445419
6	42	-0.004893305017681371
6	43	0.025719478763789994
6	44	0.0067828554334070645
6	45	-0.004101120938756253
6	46	-0.014581178877016043
6	47	-0.0287661383581721
6	48	-0.0520940768414917
6	49	-0.01800128877395224
6	50	0.001955925471790465
7	1	0.030472400163468194
7	2	0.011983373620502391
7	3	0.02676545942872366
7	4	0.020792878106476564
7	5	0.011312681494075605
7	6	0.02346129609032488
7	7	0.020544343339778948
7	8	0.009917641863822269
7	9	0.02435447943996394
7	10	0.03190699831430521
7	11	-0.0034313647660653146
7	12	-0.01658264487197414
7	13	-9.566109427794345E-5
7	14	-0.013314042718477026
7	15	-0.022176298624764
7	16	-0.01598871981371275
7	17	-0.0189619605607092
7	18	-0.014326405444982981
7	19	0.030161080766127668
7	20	-0.028361199006425863
7	21	-0.01040927398078395
7	22	-0.015954655347059088
7	23	-0.0418401620981399
7	24	-0.025714239162851982
7	25	-0.015078246509951043
7	26	-0.039444570286839564
7	27	-0.06587388558417473
7	28	-0.036499762459321516
7	29	-0.03630023908738167
7	30	-0.0021220888991990705
7	31	-0.027339243379458367
7	32	-0.006315850395580469
7	33	0.008851555703849101
7	34	0.010065495683527104
7	35	-0.027760153027273304
7	36	-0.03689389852829095
7	37	-0.004382290621428808
7	38	-0.03355736258502162
7	39	-0.005308769966177351
7	40	-9.007819067008427E-4
7	41	0.0010179814889923477
7	42	-0.007282423211975697
7	43	0.007936046914323924
7	44	-0.03579008966788422
7	45	-0.010414699977609132
7	46	-0.041072776355434826
7	47	-0.03760159148660591
7	48	-0.0878926032064058
7	49	-0.08274538132150155
7	50	-0.06593278772673017
8	1	0.031338637857658114
8	2	-0.015259906832817194
8	3	0.002603928480127138
8	4	-5.170340430851184E-4
8	5	0.019262397419396393
8	6	0.012282214849626882
8	7	0.030328862870646844
8	8	0.011762980083418029
8	9	0.021934573623155984
8	10	0.00812499464421279
8	11	-9.09173816268094E-5
8	12	-0.035152882555358644
8	13	-0.01828027941809296
8	14	-0.015210610653689116
8	15	-0.011513263513236893
8	16	-0.038914383078264336
8	17	-0.03340433532214604
8	18	-0.036402720138568156
8	19	0.06025987895036167
8	20	-0.032988031485665203
8	21	-0.014284437379771475
8	22	-0.015021254802142892
8	23	-0.005684766868668589
8	24	-0.013485051848718399
8	25	0.016522015348314767
8	26	-0.0012474656336607381
8	27	0.0034842325093720206
8	28	0.007502289428565234
8	29	0.03429577113561777
8	30	0.024578302778500927
8	31	0.0337411236539964
8	32	0.05625803248920391
8	33	0.036542669723161225
8	34	0.05595982293257862
8	35	0.018306701957804705
8	36	0.0021650950104259437
8	37	0.0170904394241731
8	38	0.021467981971838412
8	39	-0.019331091051313365
8	40	0.04678467341281589
8	41	0.024504809772388114
8	42	0.04294127425610128
8	43	0.028296081782777094
8	44	0.059635031120272686
8	45	-0.03381498322001164
8	46	0.012711039568195304
8	47	0.04251081172053972
8	48	-0.003055200662835489
8	49	0.009186165281306558
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112	35	-0.028625933288012106
112	36	-0.0030347686272875762
112	37	0.005878757358921405
112	38	0.04987626332336248
112	39	0.009555577984492913
112	40	-0.021890260770469183
112	41	-0.0017546349862271882
112	42	-0.020743147738599532
112	43	0.019800370180725224
112	44	0.0405831519380655
112	45	0.04740534554076703
112	46	0.042352446968742186
112	47	0.024335717302257365
112	48	0.047644331616304214
112	49	0.07092389947316491
112	50	0.08646185783671001
113	1	0.02672843885810039
113	2	0.006493503199315853
113	3	0.0016433899698355958
113	4	0.04470613493329978
113	5	0.035887059773799024
113	6	0.001062098952310464
113	7	0.013726389879721523
113	8	-0.010535592681861772
113	9	0.042404313850092024
113	10	0.017517242627860696
113	11	-0.021020498988789882
113	12	-0.005278030685161639
113	13	-0.020644538570877558
113	14	-0.030144282940561595
113	15	-0.018615270812126994
113	16	-0.02689530030955467
113	17	-0.036552088406892835
113	18	-0.05265217045228354
113	19	0.06478410352812602
113	20	-0.026141862424900353
113	21	-0.03384773896341642
113	22	-0.02735425400386049
113	23	-0.07591713093513164
113	24	-0.05527208989564514
113	25	-0.016254047115076276
113	26	-0.019339154372254086
113	27	7.878758452761758E-4
113	28	-0.02571781793692196
113	29	0.02628911064781647
113	30	0.04679199456300864
113	31	0.02292286380960462
113	32	0.06434258192667386
113	33	0.053998320294446955
113	34	0.08388435946876172
113	35	0.0786115501998097
113	36	0.055311118960489125
113	37	0.09167596895627383
113	38	0.03360326307053896
113	39	0.05420969602442938
113	40	0.08137327764115554
113	41	0.08800894063080733
113	42	0.06734662927237878
113	43	0.10424367335220097
113	44	0.0635849235532362
113	45	0.13571198436689258
113	46	0.15167996434575315
113	47	0.14951214036222638
113	48	0.13410898023192885
113	49	0.17566388234325103
113	50	0.2229193633933022
114	1	-0.023759951046514516
114	2	-0.02518864314242819
114	3	-0.023789767137181173
114	4	0.0075693688949414195
114	5	-0.015011938429367433
114	6	-0.032749968583864586
114	7	-0.004441094729315864
114	8	-0.026367752550207513
114	9	-0.013918888567935994
114	10	-0.02380412720557956
114	11	-0.024836334277480887
114	12	-0.0320450588637442
114	13	-0.05540845996865329
114	14	-0.049990963534632726
114	15	-0.04988685961123451
114	16	-0.05220628088103041
114	17	-0.04929735815093039
114	18	-0.06298378921041348
114	19	0.02758652626469882
114	20	-0.030543480181698612
114	21	-0.04130368833526887
114	22	-0.056892574850401445
114	23	-0.041181197194518404
114	24	-0.02665328293388569
114	25	-0.043860356766650455
114	26	-0.04259011733250162
114	27	-0.0542643674571579
114	28	-0.06546376930187847
114	29	0.009387739374432158
114	30	0.01895834212113101
114	31	0.061750329097051804
114	32	0.05289655503155899
114	33	0.03660873915895735
114	34	0.050302238783288544
114	35	0.04158500908902596
114	36	0.04375234767060476
114	37	0.03708356154987058
114	38	0.05624464749811153
114	39	0.05477084072573035
114	40	0.05648738067306169
114	41	0.05933078308640205
114	42	0.08972852267738318
114	43	0.08775561876935711
114	44	0.09145089341376433
114	45	0.10426654528166779
114	46	0.15083200925093365
114	47	0.16553480691595013
114	48	0.21166571503097487
114	49	0.19470984045056383
114	50	0.23572589411620726
115	1	0.0020162329402637624
115	2	-0.027912130017142545
115	3	-0.015424046671839164
115	4	0.03195903453828208
115	5	0.011476619919577047
115	6	-0.025705171630754364
115	7	-0.013182149561643541
115	8	-0.027538258529979487
115	9	-0.008544806001786753
115	10	-0.002289834977574401
115	11	-0.06090157800460361
115	12	-0.04947692594534914
115	13	-0.03729863310145021
115	14	-0.029920129018414343
115	15	-0.028482645617764035
115	16	-0.04788128437117933
115	17	-0.028933302094095836
115	18	-0.021429471295469682
115	19	0.03693541521849397
115	20	-0.018640708022516606
115	21	-0.018598486772688716
115	22	-0.0270619533831038
115	23	-0.019799407833183125
115	24	-0.015335764460147061
115	25	-0.005703437392945432
115	26	0.009282203678637302
115	27	0.02557554993828859
115	28	-0.049639489598447994
115	29	0.0575241291921742
115	30	0.09382668935947436
115	31	0.0806488394576732
115	32	0.10445782191153796
115	33	0.07074908974543348
115	34	0.01086063421416128
115	35	0.03672763623195152
115	36	0.06509157613739092
115	37	0.07394021255637284
115	38	0.05446762755666157
115	39	0.0653924138016535
115	40	0.058841841847467435
115	41	0.09124057415544229
115	42	0.07266657577739011
115	43	0.10517953428785631
115	44	0.1098422509649879
115	45	0.1091818493658252
115	46	0.17119177513621509
115	47	0.1527391323681826
115	48	0.19385186131248133
115	49	0.21302293297521402
115	50	0.2394999569219749
116	1	-0.007977203235078889
116	2	-0.017371954646122845
116	3	-0.03852951808165983
116	4	-0.012266413690475986
116	5	-0.012678928850540672
116	6	-0.03820016598681519
116	7	-0.04651440402401619
116	8	-0.02694636759984803
116	9	-0.014992526201922374
116	10	-0.013350899314637843
116	11	-0.040473654233117884
116	12	-0.020492089246914702
116	13	-0.031068415912905323
116	14	-0.026344858981723007
116	15	-0.03612729436637352
116	16	-0.0186666842665133
116	17	-0.028645442690276468
116	18	-0.029025910049661263
116	19	0.06232097536009462
116	20	-0.01494790560836634
116	21	-0.015417212532419455
116	22	-0.01664239600425077
116	23	-0.001929606374545756
116	24	-0.01079790846473827
116	25	0.024045739989830395
116	26	0.018289857075430405
116	27	0.028498305469256024
116	28	0.03452838215395104
116	29	0.08062387799031256
116	30	0.1085410120980086
116	31	0.12840399566757554
116	32	0.13707466784914146
116	33	0.09776456567885106
116	34	0.1274546291833829
116	35	0.10713542800871778
116	36	0.0794830965267721
116	37	0.11724124016016901
116	38	0.11328540732382208
116	39	0.13025925125381121
116	40	0.10963741726854437
116	41	0.09431299841284613
116	42	0.14378620902078865
116	43	0.14646002587836193
116	44	0.12935792697098236
116	45	0.13232967685563324
116	46	0.16042231233145543
116	47	0.12820795377431438
116	48	0.10888652228516094
116	49	0.19877772215015455
116	50	0.16238063035804373
117	1	-0.16979665036718927
117	2	-0.21526354335703246
117	3	-0.22438946401310034
117	4	-0.20877770904547788
117	5	-0.22672451051622033
117	6	-0.3545538243751949
117	7	-0.2784800109410952
117	8	-0.3874935022374473
117	9	-0.3213329645971399
117	10	-0.5603088351901206
117	11	-0.49534749003836964
117	12	-0.5083280328578113
117	13	-0.4363067463876291
117	14	-0.7858042689018063
117	15	-0.6530594125928317
117	16	-0.607769191097205
117	17	-0.823039959925481
117	18	-0.798325360589935
117	19	-0.7263602084589778
117	20	-0.7396296956053234
117	21	-0.6854997897459825
117	22	-0.5013385077717487
117	23	-0.6820709103260825
117	24	-0.8025643026785474
117	25	-0.7384198695314552
117	26	-0.858539360485004
117	27	-0.5992170916760813
117	28	-0.545090509041323
117	29	-0.5161435772685152
117	30	-0.5856362680236131
117	31	-0.4771648413135807
117	32	-0.530451351792415
117	33	-0.5472132291664593
117	34	-0.5195002551026136
117	35	-0.5909326454073209
117	36	-0.5928188609910947
117	37	-0.564411752447505
117	38	-0.5952894823548718
117	39	-0.6018387024985188
117	40	-0.5942212642231013
117	41	-0.56579044713218
117	42	-0.5403743454523564
117	43	-0.5031460352861785
117	44	-0.4814961862177469
117	45	-0.46609276489406426
117	46	-0.44540774605206224
117	47	-0.42795473120242633
117	48	-0.41126189781280686
117	49	-0.40986123421425447
117	50	-0.3773465074606861
118	1	-0.02651387462503152
118	2	-0.04216978907757607
118	3	-0.04671611712497281
118	4	-0.022575597102793665
118	5	-0.03718391110475494
118	6	-0.03912630458199118
118	7	-0.03736837798874859
118	8	-0.04439365418149066
118	9	-0.021402898403927395
118	10	-0.01524143051575777
118	11	-0.0669768450567716
118	12	-0.06885415885556512
118	13	-0.0725724461554762
118	14	-0.08057890865976702
118	15	-0.08080121437443921
118	16	-0.08879998441462789
118	17	-0.09528750615500314
118	18	-0.09744243844897937
118	19	-0.03743352965814495
118	20	-0.07997050695622931
118	21	-0.10579968068225298
118	22	-0.0990063023345158
118	23	-0.11688261769799624
118	24	-0.10463713177816203
118	25	-0.10988625538434604
118	26	-0.12353728728277247
118	27	-0.08755946898646982
118	28	-0.16576364991656334
118	29	-0.10592246420403484
118	30	-0.10341749060720673
118	31	-0.11293808201410371
118	32	-0.12329188070628305
118	33	-0.09300902724418592
118	34	-0.13743886620092383
118	35	-0.1513649570975346
118	36	-0.17925766715344338
118	37	-0.1917477327905388
118	38	-0.18345616312976887
118	39	-0.16280242886763574
118	40	-0.17021050743020538
118	41	-0.14916436206464567
118	42	-0.1321997351964015
118	43	-0.132565556636834
118	44	-0.1323083002772094
118	45	-0.11369090073337418
118	46	-0.09533645861744589
118	47	-0.1124348215430544
118	48	-0.1006327898791568
118	49	-0.06759645741904308
118	50	-0.06553849590130767
119	1	-0.0011382367560841544
119	2	-0.01793415524635833
119	3	-0.020554627116517565
119	4	0.02307700015534664
119	5	5.045019500826697E-4
119	6	-0.012852629842463159
119	7	-0.0138149296311596
119	8	-0.016407822810030837
119	9	0.02506702214322587
119	10	-0.002292975888565252
119	11	-0.05227465685847932
119	12	-0.06676074414190225
119	13	-0.10573435374568163
119	14	-0.06929790167079375
119	15	-0.05795699130609222
119	16	-0.07539905894483923
119	17	-0.060728559293160345
119	18	-0.059781271971296235
119	19	0.03288984004046114
119	20	-0.04834464110715686
119	21	-0.046010673948643444
119	22	-0.10302584380224289
119	23	-0.0713663988245159
119	24	-0.06386708081552683
119	25	-0.06797614167391686
119	26	-0.07013312561188911
119	27	-0.06099590690058676
119	28	-0.06773555265603193
119	29	0.03757659412474368
119	30	-0.05185858895210771
119	31	0.13110121615756753
119	32	0.11732325991084025
119	33	0.13064728115398339
119	34	0.14234389020771232
119	35	0.09034486083157134
119	36	0.08568453674035226
119	37	0.07616724778038275
119	38	0.08911173589442001
119	39	0.10971145178802288
119	40	0.09274639761797232
119	41	0.020635188742062383
119	42	0.09695618274152196
119	43	0.04598759651121043
119	44	0.10152726451097038
119	45	0.09541317774534974
119	46	0.035233325408377425
119	47	-0.028185916173143255
119	48	0.02471457781534525
119	49	0.0406293720348927
119	50	0.17321319478913466
120	1	-0.06220720458667017
120	2	-0.06819855983277279
120	3	-0.07595178861411256
120	4	-0.05795828558322569
120	5	-0.050249914666537165
120	6	-0.06485360873982415
120	7	-0.05997754891851059
120	8	-0.06078232704047082
120	9	-0.0463343785368906
120	10	-0.04973145528352774
120	11	-0.07892718804185961
120	12	-0.08036331887059589
120	13	-0.09785351812912779
120	14	-0.09345499893895948
120	15	-0.0845153277189965
120	16	-0.1081271901317109
120	17	-0.12015771063013858
120	18	-0.11757493452116563
120	19	-0.04292630627284666
120	20	-0.12222387760630937
120	21	-0.11023775948327597
120	22	-0.11635343009056953
120	23	-0.09210342902949264
120	24	-0.10300205316919886
120	25	-0.11067648010578779
120	26	-0.14034869367197444
120	27	-0.10127759836139205
120	28	-0.11349871717172988
120	29	0.01258678270239244
120	30	0.02388191576695675
120	31	0.10384568995804244
120	32	0.08037455907378188
120	33	0.07806614335382989
120	34	0.10506271331866657
120	35	0.07501612440300676
120	36	-0.010390472978897947
120	37	0.025195759374895488
120	38	0.012981054857206686
120	39	0.037483324857973344
120	40	0.019624852988485486
120	41	0.05248305696495237
120	42	0.0384139836597015
120	43	0.04941744941418591
120	44	0.07607111659760335
120	45	0.052020257041967
120	46	0.11620429620524675
120	47	0.15987150315025644
120	48	0.19580215709697057
120	49	0.15943325379959816
120	50	0.14159556801369888
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	24891.0
3	2525.0
4	2734.0
5	1360.0
6	1862.0
7	2634.0
8	2798.0
9	3578.0
10	5206.0
11	6404.0
12	6960.0
13	8851.0
14	11060.0
15	13339.0
16	15227.0
17	18051.0
18	20566.0
19	25388.0
20	31544.0
21	29701.0
22	38072.0
23	55514.0
24	74702.0
25	67876.0
26	105465.0
27	135462.0
28	151172.0
29	154017.0
30	193606.0
31	238837.0
32	297271.0
33	351549.0
34	502573.0
35	734338.0
36	1094899.0
37	1898119.0
38	3610250.0
39	1.7307051E7
40	392146.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.52978765288846	19.03515293674147	12.327360561662601	23.10769884870747
2	16.974988202665077	22.26020510176029	36.441176979272946	24.323629716301685
3	18.246849816688123	26.574150908483436	32.224424857760795	22.954574417067647
4	11.599115995068274	15.973543543250127	37.87780191529577	34.54953854638583
5	11.930234734153384	40.39088877791237	35.889750866558806	11.789125621375437
6	30.58041440504345	37.344218553291064	18.17029106509184	13.905075976573652
7	27.056443182942306	34.06860827775265	22.390570989562843	16.484377549742202
8	29.392843763050607	33.36524035120563	20.71216174430209	16.529754141441668
9	27.60887903500152	12.666165851316022	19.883699733963855	39.841255379718596
10	17.46027277768495	28.023831882929912	34.39814849322289	20.117746846162245
11	35.285439783877024	21.69602437954268	23.395430384362637	19.623105452217665
12	21.026552307476216	27.07127804666672	32.19153487940595	19.710634766451122
13	29.505686420361133	20.63331625273658	26.472962664845184	23.388034662057102
14	20.58753079771983	20.59004548803409	30.283836533116954	28.538587181129127
15	24.248131114722778	28.774302310931652	24.770571595983125	22.206994978362445
16	22.858980002531336	27.304644926089455	26.781802818030716	23.0545722533485
17	20.352685440734682	27.985197125904936	28.34441016010515	23.31770727325523
18	20.625536520868074	27.68794128134707	29.679257083341852	22.007265114443012
19	21.58129718121567	27.20490659315133	30.41646398376009	20.797332241872915
20	23.325474956253434	25.67754259975849	30.24613426970028	20.750848174287796
21	23.548791606903517	25.670692985284415	29.6282549577168	21.15226045009527
22	21.88355153005699	25.694106991497595	30.027956843427567	22.394384635017847
23	21.731595425439934	27.018708872711755	29.835471308266403	21.414224393581907
24	22.497220740408796	26.04633939975809	29.986931816824846	21.46950804300827
25	21.57212741432553	27.08579865384816	30.021503287801615	21.3205706440247
26	21.378785238899113	25.867189391189534	29.933083871359173	22.820941498552184
27	20.999509465860815	26.06119187125198	29.983064806619137	22.956233856268067
28	22.493580917824783	26.020072048924263	30.083822776397323	21.40252425685363
29	21.11425864991272	27.258285913083807	30.160552463080485	21.466902973922984
30	21.19506845964266	26.307445472371356	30.99436278562845	21.503123282357535
31	21.473819870143977	27.00439670999749	29.9331732943779	21.58861012548063
32	21.085262395946437	26.187043069127757	31.245877669200144	21.481816865725662
33	21.16309197537602	26.23750459993632	30.073314794209754	22.526088630477904
34	22.72092901775567	25.748271661253753	30.158093442095797	21.37270587889478
35	21.224799955822352	26.153642257007093	31.278535547164623	21.343022240005936
36	21.58225993699929	27.68647340506393	29.482466851205867	21.248799806730915
37	21.604330575057762	26.18964683259226	30.86432069236613	21.341701899983846
38	22.74052811815039	26.74566500974857	29.349605589602174	21.16420128249887
39	21.34296110704616	26.66295966366266	29.466536472315656	22.52754275697552
40	21.306680359007377	26.34874355567992	29.675891790662945	22.668684294649754
41	22.46180974104883	26.43110506800576	29.40178146061953	21.70530373032588
42	21.103501181391334	26.694159723528895	30.59775848040133	21.604580614678444
43	21.420531476482278	26.40134517778999	29.383645858897438	22.794477486830292
44	21.481005444144746	26.63087346734797	30.451120588362183	21.437000500145096
45	21.291526398140377	26.79939893428213	30.393722237914083	21.515352429663405
46	21.642326123054495	26.84979640080997	28.73787186820928	22.770005607926254
47	21.415171691539168	27.00418164822907	30.146830146649055	21.433816513582705
48	22.847525732017644	27.083159058506727	28.279533562706167	21.78978164676946
49	21.660722830277624	26.925198624175028	28.567925908292384	22.846152637254967
50	21.6831807991199	27.11425255096617	29.490426798698117	21.71213985121581
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	62466.0
1	37987.5
2	13509.0
3	33501.0
4	53493.0
5	54335.5
6	55178.0
7	41163.5
8	27149.0
9	37893.5
10	48638.0
11	73829.0
12	99020.0
13	164060.5
14	229101.0
15	226002.0
16	222903.0
17	280043.0
18	337183.0
19	315199.5
20	293216.0
21	318352.0
22	343488.0
23	383856.0
24	424224.0
25	470651.0
26	517078.0
27	587956.5
28	658835.0
29	728982.5
30	799130.0
31	884664.0
32	970198.0
33	1050361.5
34	1130525.0
35	1214579.0
36	1298633.0
37	1366170.5
38	1433708.0
39	1475944.5
40	1518181.0
41	1540692.0
42	1563203.0
43	1587378.5
44	1611554.0
45	1610041.5
46	1608529.0
47	1574988.0
48	1541447.0
49	1519150.5
50	1496854.0
51	1463627.0
52	1430400.0
53	1401122.5
54	1371845.0
55	1314575.0
56	1257305.0
57	1290580.5
58	1323856.0
59	1226340.5
60	1128825.0
61	971706.5
62	814588.0
63	710620.0
64	606652.0
65	528600.5
66	450549.0
67	383736.5
68	316924.0
69	271130.5
70	225337.0
71	187074.0
72	148811.0
73	121078.5
74	93346.0
75	73364.5
76	53383.0
77	41538.0
78	29693.0
79	22374.5
80	15056.0
81	11186.5
82	7317.0
83	5457.0
84	3597.0
85	2576.0
86	1555.0
87	1079.0
88	603.0
89	382.0
90	161.0
91	111.0
92	61.0
93	53.0
94	45.0
95	48.5
96	52.0
97	71.0
98	90.0
99	97.0
100	104.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1409854792735606
2	0.0
3	0.0
4	0.01544273131116532
5	4.3419113339733794E-4
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0038823923844611965
18	1.555851561340461E-4
19	3.6182594449778166E-5
20	0.0
21	9.769300501440103E-5
22	0.0
23	8.321996723448977E-4
24	0.009190378990243653
25	0.0032347239438101677
26	0.04926260234337297
27	0.05300026435003505
28	0.03410933178780587
29	0.010691956659909446
30	0.005818161187524328
31	0.029217445018195863
32	0.027491535262941447
33	0.054031468291853735
34	0.04790213679206131
35	0.07896127586775088
36	0.05791024241686995
37	0.04168596706558942
38	0.10096752981210595
39	0.12948303249797613
40	0.11188743681704902
41	0.08996078458048344
42	0.10204577112670934
43	0.09866993506454504
44	0.11436956279630378
45	0.09398428908329877
46	0.07717385570193183
47	0.09666180107258236
48	0.08382783482124605
49	0.07915304361833471
50	0.09478754268008385
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2.7637598E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.135036391050264
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.08392586945504	26.047552845025272
2	11.253734585549825	8.133082175681276
3	5.106942906699185	5.536187033417732
4	2.889223218707495	4.176087445994508
5	1.9275171495614192	3.482545117188768
6	1.2614352105943385	2.7349204343867144
7	0.9369193065236431	2.369892926569659
8	0.6730760905048971	1.9457303219472237
9	0.5244086410734211	1.7054572796072358
>10	2.9557730246882477	19.215141646854487
>50	0.21755015096688074	5.479283753600394
>100	0.15370051958474254	10.65376720385354
>500	0.011347819030268887	2.7760574683480206
>1k	0.0041963796516105295	2.4878572027787134
>5k	1.295462526440704E-4	0.2941495999380772
>10k+	1.1958115627078461E-4	2.9622875448084116
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	200058	0.7238617480433719	Illumina PCR Primer Index 1 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	122505	0.4432548733070074	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	115987	0.41967105824464196	No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT	106062	0.38375983325323715	Illumina Paired End PCR Primer 2 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	74659	0.27013563190259876	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	53834	0.19478537896093576	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40385	0.1461234076854291	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39303	0.1422084509659631	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33520	0.12128405659565639	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	3.618259444977816E-6	0.0	0.0	0.0
4	0.0	3.618259444977816E-6	0.0	3.618259444977816E-6	0.0
5	0.0	1.0854778334933447E-5	0.0	1.0854778334933447E-5	0.0
6	3.618259444977816E-6	1.0854778334933447E-5	0.0	1.0854778334933447E-5	0.0
7	7.236518889955632E-6	1.0854778334933447E-5	0.0	1.4473037779911264E-5	0.0
8	2.5327816114844714E-5	1.0854778334933447E-5	0.0	2.1709556669866895E-5	0.0
9	2.5327816114844714E-5	1.0854778334933447E-5	0.0	3.618259444977816E-5	0.0
10	2.894607555982253E-5	1.0854778334933447E-5	0.0	6.151041056462288E-5	0.0
11	2.894607555982253E-5	1.4473037779911264E-5	0.0	6.512867000960069E-5	0.0
12	3.618259444977816E-5	2.5327816114844714E-5	0.0	7.236518889955632E-5	3.618259444977816E-6
13	6.512867000960069E-5	2.894607555982253E-5	0.0	8.683822667946758E-5	3.618259444977816E-6
14	1.6643993446897954E-4	2.894607555982253E-5	0.0	8.683822667946758E-5	1.0854778334933447E-5
15	1.77294712803913E-4	3.9800853894755975E-5	0.0	8.683822667946758E-5	1.0854778334933447E-5
16	1.9538601002880206E-4	3.9800853894755975E-5	0.0	8.683822667946758E-5	1.0854778334933447E-5
17	1.9538601002880206E-4	4.341911333973379E-5	0.0	1.0131126445937885E-4	1.0854778334933447E-5
18	1.9538601002880206E-4	4.703737278471161E-5	0.0	1.8814949113884645E-4	1.0854778334933447E-5
19	1.9900426947377987E-4	4.703737278471161E-5	0.0	1.9538601002880206E-4	1.4473037779911264E-5
20	2.026225289187577E-4	4.703737278471161E-5	0.0	2.026225289187577E-4	1.4473037779911264E-5
21	2.1347730725369116E-4	4.703737278471161E-5	0.0	2.0624078836373552E-4	1.4473037779911264E-5
22	2.1709556669866897E-4	5.065563222968943E-5	0.0	2.3518686392355804E-4	1.4473037779911264E-5
23	2.1709556669866897E-4	5.065563222968943E-5	0.0	2.3518686392355804E-4	1.4473037779911264E-5
24	2.2071382614364678E-4	5.065563222968943E-5	0.0	2.3880512336853587E-4	1.809129722488908E-5
25	2.2071382614364678E-4	5.427389167466724E-5	0.0	2.4242338281351368E-4	1.809129722488908E-5
26	2.460416422584915E-4	6.151041056462288E-5	0.0	2.5327816114844714E-4	1.809129722488908E-5
27	2.6413293948338056E-4	6.874692945457851E-5	0.0	3.184068311580478E-4	1.809129722488908E-5
28	2.8584249615324747E-4	6.874692945457851E-5	0.0	4.739919872920939E-4	2.1709556669866895E-5
29	2.8584249615324747E-4	7.598344834453414E-5	0.0	6.87469294545785E-4	2.5327816114844714E-5
30	2.894607555982253E-4	7.598344834453414E-5	0.0	9.733117906990325E-4	2.5327816114844714E-5
31	3.0031553393315875E-4	7.598344834453414E-5	0.0	0.0012482995085173465	2.5327816114844714E-5
32	3.0031553393315875E-4	7.960170778951195E-5	0.0	0.0016499263069098841	2.5327816114844714E-5
33	3.0031553393315875E-4	7.960170778951195E-5	0.0	0.0024459433848050037	2.894607555982253E-5
34	3.0031553393315875E-4	9.04564861244454E-5	0.0	0.0035097116616284816	2.894607555982253E-5
35	3.0031553393315875E-4	9.407474556942322E-5	0.0	0.005156019709093388	2.894607555982253E-5
36	3.0393379337813656E-4	9.407474556942322E-5	0.0	0.008148320270090041	2.894607555982253E-5
37	3.0393379337813656E-4	1.0131126445937885E-4	0.0	0.01393391712260957	2.894607555982253E-5
38	3.0755205282311437E-4	1.0131126445937885E-4	0.0	0.022208876473273836	2.894607555982253E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	40170	0.0	36.654984	35
CCGTCTT	40280	0.0	36.37462	39
GCCGTCT	40335	0.0	36.336006	38
GGTATCA	47905	0.0	36.06158	1
CGTCTTC	40680	0.0	35.943653	40
GACCGAT	40860	0.0	35.492657	24
TCGTATG	41475	0.0	35.325665	32
ATGCCGT	41480	0.0	35.263798	36
TGCCGTC	41720	0.0	35.09788	37
CGTATGC	41965	0.0	35.067467	33
TGAGCGG	43865	0.0	34.802547	5
ATATCGT	26135	0.0	34.752464	29
ACCGATA	26365	0.0	34.629444	25
GCGGGCT	44125	0.0	34.57244	8
TATCGTA	26315	0.0	34.55743	30
AGACCGA	42910	0.0	34.545555	23
ATCGTAT	26680	0.0	34.505257	31
CCGATAT	26585	0.0	34.284943	26
CGATATC	26640	0.0	34.262283	27
GATATCG	26750	0.0	34.154102	28
>>END_MODULE