SRR522085_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR522085_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29081401
Sequences flagged as poor quality	0
Sequence length	50
%GC	45

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	160622	0.5523186451711869	Illumina PCR Primer Index 1 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	119114	0.40958824507801395	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	112982	0.3885026034337204	No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT	85870	0.2952746327455132	Illumina Paired End PCR Primer 2 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	71823	0.246972283075358	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38487	0.13234231734571522	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38300	0.1316992946797852	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32496	0.11174152166878067	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	29419	0.10116087598393214	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	33320	36.25129	35
CCGTCTT	33790	35.507534	39
GACCGAT	33515	35.443542	24
TCGTATG	33940	35.349194	32
GCCGTCT	34075	35.24884	38
GGTATCA	45850	34.829357	1
CGTATGC	34785	34.664845	33
CGTCTTC	34540	34.66475	40
TATCGTA	21800	34.631657	30
ATATCGT	21850	34.54979	29
ACCGATA	22145	34.48237	25
AGACCGA	35515	34.401833	23
ATCGTAT	22425	34.26419	31
ATGCCGT	35050	34.261326	36
GCGGGCT	37015	33.893948	8
CCGATAT	22385	33.87684	26
CGATATC	22390	33.847668	27
GATATCG	22435	33.784252	28
TGAGCGG	37255	33.764626	5
TGCCGTC	35945	33.457813	37