SRR522084_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR522084_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26822612
Sequences flagged as poor quality	0
Sequence length	50
%GC	44

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	172008	0.6412798276319994	Illumina PCR Primer Index 1 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	101213	0.37734207242754736	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	94946	0.35397745752725346	No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT	87000	0.3243531987115945	Illumina Paired End PCR Primer 2 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59811	0.22298723181769176	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	40784	0.15205081443969737	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32947	0.12283292917184949	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32594	0.12151687538857141	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27453	0.10235021108309661	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	33915	36.870914	35
CCGTCTT	34415	36.22091	39
GCCGTCT	34550	35.989403	38
CGTCTTC	34960	35.62342	40
ATGCCGT	35420	35.12261	36
TCGTATG	35510	35.019547	32
CGTATGC	35835	34.83933	33
GGTATCA	39975	34.784687	1
TATCGTA	22695	34.771786	30
ATATCGT	22720	34.733124	29
GACCGAT	35410	34.538097	24
ATCGTAT	23155	34.50366	31
TGCCGTC	36270	34.26286	37
GATATCG	23205	34.1218	28
GCGGGCT	38165	34.024975	8
TGAGCGG	38315	33.824894	5
AGACCGA	37055	33.81558	23
CGATATC	23465	33.73135	27
ACCGATA	23395	33.71826	25
CCGATAT	23580	33.49431	26