FastQCFastQC Report
Thu 26 May 2016
SRR522084_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522084_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26822612
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT1720080.6412798276319994Illumina PCR Primer Index 1 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1012130.37734207242754736No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT949460.35397745752725346No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT870000.3243531987115945Illumina Paired End PCR Primer 2 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT598110.22298723181769176No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA407840.15205081443969737No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT329470.12283292917184949No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT325940.12151687538857141No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274530.10235021108309661No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG339150.036.87091435
CCGTCTT344150.036.2209139
GCCGTCT345500.035.98940338
CGTCTTC349600.035.6234240
ATGCCGT354200.035.1226136
TCGTATG355100.035.01954732
CGTATGC358350.034.8393333
GGTATCA399750.034.7846871
TATCGTA226950.034.77178630
ATATCGT227200.034.73312429
GACCGAT354100.034.53809724
ATCGTAT231550.034.5036631
TGCCGTC362700.034.2628637
GATATCG232050.034.121828
GCGGGCT381650.034.0249758
TGAGCGG383150.033.8248945
AGACCGA370550.033.8155823
CGATATC234650.033.7313527
ACCGATA233950.033.7182625
CCGATAT235800.033.4943126