##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522084_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26822612
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.93242473924612	40.0	39.0	40.0	38.0	40.0
2	38.799125156043715	40.0	39.0	40.0	38.0	40.0
3	38.743849592276845	40.0	39.0	40.0	37.0	40.0
4	38.7885059814458	40.0	39.0	40.0	37.0	40.0
5	38.77425386461244	40.0	39.0	40.0	37.0	40.0
6	38.6080419386449	40.0	39.0	40.0	37.0	40.0
7	38.72309930144014	40.0	39.0	40.0	36.0	40.0
8	38.74752581888744	40.0	39.0	40.0	37.0	40.0
9	38.774260090702576	40.0	39.0	40.0	37.0	40.0
10	38.42693485630706	40.0	39.0	40.0	36.0	40.0
11	38.78086966325278	40.0	39.0	40.0	37.0	40.0
12	38.5377616840597	40.0	39.0	40.0	36.0	40.0
13	38.739578680853306	40.0	39.0	40.0	36.0	40.0
14	38.709956099726604	40.0	39.0	40.0	36.0	40.0
15	38.700616256164764	40.0	39.0	40.0	36.0	40.0
16	38.75215202755049	40.0	39.0	40.0	36.0	40.0
17	38.72565777710239	40.0	39.0	40.0	36.0	40.0
18	38.706137493246366	40.0	39.0	40.0	36.0	40.0
19	38.68117687419853	40.0	39.0	40.0	36.0	40.0
20	38.64734493419209	40.0	39.0	40.0	36.0	40.0
21	38.60133080998972	40.0	39.0	40.0	36.0	40.0
22	38.55829760352944	40.0	39.0	40.0	36.0	40.0
23	38.50419537813841	40.0	39.0	40.0	36.0	40.0
24	38.46403575460884	40.0	38.0	40.0	35.0	40.0
25	38.39555133556717	40.0	38.0	40.0	35.0	40.0
26	38.233088149655224	40.0	38.0	40.0	35.0	40.0
27	38.19065365446139	40.0	38.0	40.0	35.0	40.0
28	38.07124470204468	40.0	38.0	40.0	35.0	40.0
29	38.00305838969001	40.0	38.0	40.0	35.0	40.0
30	38.08595109976612	40.0	38.0	40.0	35.0	40.0
31	37.56989874811595	40.0	38.0	40.0	34.0	40.0
32	37.55507882677496	40.0	38.0	40.0	34.0	40.0
33	37.4876674203094	40.0	38.0	40.0	33.0	40.0
34	37.437123200380334	40.0	38.0	40.0	33.0	40.0
35	37.27170802754035	40.0	38.0	40.0	33.0	40.0
36	37.42474711262274	40.0	38.0	40.0	35.0	40.0
37	37.28135522371945	40.0	38.0	40.0	34.0	40.0
38	37.21665235287302	40.0	38.0	40.0	33.0	40.0
39	37.123481747415205	40.0	38.0	40.0	33.0	40.0
40	37.05150053991759	40.0	38.0	40.0	33.0	40.0
41	37.011897238046764	40.0	38.0	40.0	33.0	40.0
42	36.920320623509745	40.0	38.0	40.0	33.0	40.0
43	36.781067369576085	40.0	38.0	40.0	33.0	40.0
44	36.659013894694525	40.0	38.0	40.0	33.0	40.0
45	36.56038364198088	40.0	38.0	40.0	32.0	40.0
46	36.2601151595527	40.0	38.0	40.0	32.0	40.0
47	36.166567707872744	40.0	37.0	40.0	31.0	40.0
48	36.06404249519025	39.0	37.0	40.0	31.0	40.0
49	35.9132470394755	39.0	37.0	40.0	30.0	40.0
50	35.84424559397869	39.0	37.0	40.0	30.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-0.043484684656611705
1	2	-0.1367675612461028
1	3	-0.11110900057285988
1	4	-0.1283012979472531
1	5	-0.12938293615249563
1	6	0.116393334665851
1	7	-0.21278792148971348
1	8	-0.27885022137851934
1	9	-0.2605674934738218
1	10	0.018106705403894807
1	11	-0.4121908079741843
1	12	-0.38135740681484265
1	13	-0.5274521630716222
1	14	-0.43950534910672445
1	15	-0.4851675147509411
1	16	-0.6334215368893297
1	17	-0.7619117821681556
1	18	-0.6746806055097139
1	19	-0.6568822104805108
1	20	-0.7769980648059587
1	21	-0.6232901599277625
1	22	-0.6771602097638265
1	23	-0.7767461135214546
1	24	-0.8418683396665116
1	25	-0.8054591138617795
1	26	-0.883749342642659
1	27	-0.9125320477017169
1	28	-0.8063133910106828
1	29	-0.8515173205090676
1	30	-0.7643253311982292
1	31	-0.4285575315821859
1	32	-0.40302350761076866
1	33	-0.5374129173816584
1	34	-0.41749518514104267
1	35	-0.2522288441253906
1	36	-0.3082947588960465
1	37	-0.2837950199859165
1	38	-0.2769195520218872
1	39	-0.28790374958962417
1	40	-0.36212917698683356
1	41	-0.48520464478962566
1	42	-0.3921099694981933
1	43	-0.2873164444940741
1	44	-0.4389619931250692
1	45	-0.44276719508211926
1	46	-0.3112572297734175
1	47	-0.4442304206919161
1	48	-0.5513800977069891
1	49	-0.46989702178755266
1	50	-0.4729218629910079
2	1	-2.168815398517964
2	2	-2.891210709773077
2	3	-2.7272370730287747
2	4	-4.11227378087024
2	5	-4.9795577180871575
2	6	-4.677634681999919
2	7	-6.783912218310796
2	8	-5.425857401569836
2	9	-2.7338478722005206
2	10	-2.5295462180883703
2	11	-0.9083707327651993
2	12	-0.7457090608713557
2	13	-1.0071716201273873
2	14	-1.1129720763962538
2	15	-0.9899144771299504
2	16	-0.7715267264466092
2	17	-0.8028145429730174
2	18	-0.8123659010803266
2	19	-0.8203852873294011
2	20	-0.8569144540007869
2	21	-0.8564057866834744
2	22	-0.9061178309424989
2	23	-0.8378250209216276
2	24	-0.897341851732044
2	25	-0.8887351215065209
2	26	-0.8947485101709773
2	27	-0.9786914172896743
2	28	-0.9218135405524279
2	29	-0.9764644695874267
2	30	-0.9561169622443089
2	31	-0.4394630110984892
2	32	-0.5625073310738031
2	33	-0.5356410686445088
2	34	-0.5374632772346359
2	35	-0.33489230967035155
2	36	-0.32045107134971573
2	37	-0.3366363824044569
2	38	-0.31987357018820006
2	39	-0.2619133026426539
2	40	-0.31790916797911706
2	41	-0.4876977899592916
2	42	-0.47247648036043444
2	43	-0.46714594738024573
2	44	-0.5077715542747541
2	45	-0.663000381499323
2	46	-0.8835352431931653
2	47	-0.7994598452290731
2	48	-0.8261838744746939
2	49	-0.9033152681833201
2	50	-0.7832505223965001
3	1	-1.7587157943479284
3	2	-2.5295279388483607
3	3	-2.338950979052747
3	4	-2.295880589612473
3	5	-2.2992394283547384
3	6	-1.930069908164917
3	7	-2.85250752645878
3	8	-3.0526085691886635
3	9	-3.095086811344295
3	10	-3.243451415775951
3	11	-2.0991661556973042
3	12	-2.310552139256835
3	13	-2.243663522903063
3	14	-2.322453082178704
3	15	-2.3241969378932836
3	16	-2.1724768779741055
3	17	-2.4745791352975886
3	18	-2.4873153548804297
3	19	-2.7016840781438844
3	20	-2.352186839911276
3	21	-1.9395812867985
3	22	-1.9342781734429124
3	23	-2.1646013869760097
3	24	-2.1351968936522354
3	25	-1.9423281382154514
3	26	-2.231928906856872
3	27	-2.1321140219620958
3	28	-2.1077215376749194
3	29	-1.9074342198852605
3	30	-1.934171282081941
3	31	-1.1838684435247373
3	32	-1.1490518680460653
3	33	-1.4501469587797828
3	34	-1.466669341682838
3	35	-1.076953886419581
3	36	-1.1351819446484868
3	37	-1.0375031445702376
3	38	-1.0575554602217565
3	39	-1.2681592246010425
3	40	-1.1590662914761083
3	41	-1.152882616383593
3	42	-1.1587078693753696
3	43	-1.0788820336029588
3	44	-1.256388849874412
3	45	-1.238919927576589
3	46	-1.2362664901633238
3	47	-1.42009503492271
3	48	-1.308225209075303
3	49	-1.3719193657828512
3	50	-1.354051573990823
5	1	-1.4778413962921064
5	2	-1.5240584137138171
5	3	-1.4805152258568768
5	4	-1.5483915003437971
5	5	-1.481417531263702
5	6	-4.0194758655117795
5	7	-4.668417611523495
5	8	-2.177090305025544
5	9	-2.843212369344222
5	10	-2.187244556921925
5	11	-13.554327899350433
5	12	-36.32638696712963
5	13	-8.210795684640697
5	14	-8.497212454415056
5	15	-8.508312418809215
5	16	-5.4284261335672355
5	17	-5.8470697084494105
5	18	-6.074251501708311
5	19	-5.356316849788712
5	20	-5.041377531151213
5	21	-0.9081172266294644
5	22	-0.8949820559853521
5	23	-1.4008992889953902
5	24	-1.1388520177750792
5	25	-0.9342661910240722
5	26	-0.7587789135603273
5	27	-0.8557353321770833
5	28	-0.8729479207560402
5	29	-1.0728701523568986
5	30	-0.9226990007935356
5	31	-6.55418521635854
5	32	-7.0720268268279085
5	33	-6.596882670977283
5	34	-7.463469396497327
5	35	-34.99139332476436
5	36	-27.82709079821105
5	37	-34.99927209605968
5	38	-34.92650257539607
5	39	-34.842149863680696
5	40	-34.77452493283038
5	41	-30.03044262127875
5	42	-28.64819053062147
5	43	-34.50112952933805
5	44	-34.37545625724942
5	45	-28.09664600828465
5	46	-26.66658146879594
5	47	-26.661384672895796
5	48	-26.55463127386665
5	49	-26.40192517522692
5	50	-26.43250791831803
6	1	0.41967140718058005
6	2	0.36552497987157295
6	3	0.4087976211603319
6	4	0.38360436954304333
6	5	0.3887855582511506
6	6	0.7158197302398861
6	7	0.46235261600362065
6	8	0.40725908374515996
6	9	0.3962217392377596
6	10	0.6990863278312034
6	11	0.2873038753411876
6	12	-0.36669370632576204
6	13	0.2617920996949863
6	14	0.24769615787955246
6	15	0.23044243244090978
6	16	0.1280768191040167
6	17	0.10621913880297029
6	18	0.11840075838130559
6	19	0.09651389584450243
6	20	0.09285298080929749
6	21	0.1496591655950894
6	22	0.1347067019618109
6	23	0.15612002675686654
6	24	0.10345440026671326
6	25	0.13557470073072153
6	26	0.18606111942954584
6	27	0.1556523438481605
6	28	0.1884773695686377
6	29	0.14135077634109194
6	30	0.13380899365888865
6	31	0.16419781746053985
6	32	0.2155335188207772
6	33	0.19775786894192038
6	34	0.1169287772603056
6	35	-4.223412061542845
6	36	-1.6437891525119426
6	37	-4.677875903856595
6	38	-4.546591726196915
6	39	-5.029517648509948
6	40	-4.578657601310283
6	41	-0.9134781420596809
6	42	-0.8974601569893892
6	43	-1.0691636786278664
6	44	-0.979679587560689
6	45	-0.8335925598163456
6	46	0.5575203517397895
6	47	0.5293419137016855
6	48	0.5363477662697136
6	49	0.5165066866993797
6	50	0.5305814075283877
7	1	0.396612868593337
7	2	0.34808397597246454
7	3	0.3761898616283048
7	4	0.395438724756211
7	5	0.4118382639745377
7	6	0.7179800567299992
7	7	0.46730112034531857
7	8	0.414209673374458
7	9	0.41523491103333043
7	10	0.696125804791663
7	11	0.34207621168592794
7	12	0.5351327804660713
7	13	0.3192728708019956
7	14	0.32219833716500546
7	15	0.29670493333651393
7	16	0.19384201450702676
7	17	0.1518043742444064
7	18	0.16982299324455852
7	19	0.1517111136735494
7	20	0.17188738578015972
7	21	0.17045164949595204
7	22	0.142716344090708
7	23	0.17178647875346087
7	24	0.15790137998223486
7	25	0.18449471884723323
7	26	0.20993591863514638
7	27	0.18549434802224596
7	28	0.24336231809520825
7	29	0.1810303448201367
7	30	0.1938626005209727
7	31	0.5808778816210065
7	32	0.6632819963226879
7	33	0.6385023219228501
7	34	0.6517574018613601
7	35	0.8882584789380772
7	36	0.8153294290417179
7	37	0.8862716755600459
7	38	0.9183676112462678
7	39	0.857473810626523
7	40	0.8850333596393014
7	41	0.7945854536291463
7	42	0.7597066268342658
7	43	0.804568573828405
7	44	0.8113761447728578
7	45	0.7554581690745223
7	46	0.7353241482259492
7	47	0.702740571147018
7	48	0.6894519044117757
7	49	0.7169023280457694
7	50	0.7207788636691319
8	1	0.4226736296560887
8	2	0.35410991003182346
8	3	0.36606268439128087
8	4	0.3936994495151396
8	5	0.4007595374790185
8	6	0.7373713543841163
8	7	0.48175184645116076
8	8	0.43381487216587544
8	9	0.45452244354954274
8	10	0.7299349223644427
8	11	0.3963065385889166
8	12	0.6133586852108266
8	13	0.38285858441287957
8	14	0.3839694752753573
8	15	0.34001212282863236
8	16	0.22956193185898144
8	17	0.2109291088053098
8	18	0.19280322759479418
8	19	0.20450783074648626
8	20	0.19383240537302981
8	21	0.18438090885548064
8	22	0.17066191808709874
8	23	0.1923295759557888
8	24	0.15908290561616667
8	25	0.2044195833589697
8	26	0.22950711917157918
8	27	0.17535351993763015
8	28	0.22869990119730943
8	29	0.17045014727870011
8	30	0.23609358776678135
8	31	0.6460414191683128
8	32	0.6838857207614168
8	33	0.701212350041672
8	34	0.7117936766845716
8	35	0.9185124440244223
8	36	0.8844404903021115
8	37	0.9222785053346385
8	38	0.9587345348467835
8	39	0.9167178295698974
8	40	0.9218667012767199
8	41	0.8292912243958881
8	42	0.8015839937874958
8	43	0.850896824756795
8	44	0.875788667289001
8	45	0.7689399252561913
8	46	0.7611023258228542
8	47	0.7608909234957082
8	48	0.7248322585679716
8	49	0.7496509953582873
8	50	0.6834999190518474
9	1	0.44009619118771326
9	2	0.40178862547298166
9	3	0.4245193862164882
9	4	0.43476822419224703
9	5	0.4504968188138605
9	6	0.7423646787433498
9	7	0.5360706260814325
9	8	0.463431021066512
9	9	0.47231985906277885
9	10	0.7429563483860591
9	11	0.42145685639104613
9	12	0.611618215219444
9	13	0.3924337492781902
9	14	0.3847614929179244
9	15	0.3606124657130323
9	16	0.2623379293858932
9	17	0.23544611914968527
9	18	0.2099826195775023
9	19	0.20713631068760918
9	20	0.22114769880128193
9	21	0.22309629650453644
9	22	0.19215939061263043
9	23	0.20414511389576262
9	24	0.20299770842812848
9	25	0.22003045064277416
9	26	0.2655649923064871
9	27	0.2487876571486538
9	28	0.2606055148446771
9	29	0.23399270810955386
9	30	0.2567194053446684
9	31	0.6312340002513679
9	32	0.663728265111935
9	33	0.6653619898845236
9	34	0.6613503747970242
9	35	0.9319156437934808
9	36	0.8724639933645477
9	37	0.9490977844369013
9	38	0.975148814943104
9	39	0.9427053012649651
9	40	0.951274657222605
9	41	0.8425267009518009
9	42	0.7976515935265809
9	43	0.8378889182776845
9	44	0.8927496444406486
9	45	0.814283550827902
9	46	0.7669127914189247
9	47	0.7575148288538571
9	48	0.7734459779241476
9	49	0.765179047666777
9	50	0.7403136186984725
10	1	0.4594303750477451
10	2	0.42301692722033835
10	3	0.44243289858679447
10	4	0.4432432009960081
10	5	0.4543728320925453
10	6	0.7722029976420899
10	7	0.5212872226960812
10	8	0.47868891886277254
10	9	0.47972356178578224
10	10	0.7332843614670992
10	11	0.4147502103175853
10	12	0.6161502592612607
10	13	0.3812108816075792
10	14	0.39176981345335093
10	15	0.36430778616239934
10	16	0.2509557381327241
10	17	0.21493900391901377
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120	36	0.3804703911044811
120	37	0.4770583494848779
120	38	0.3903677835148187
120	39	0.4606038491905906
120	40	0.39294103251615553
120	41	0.35233411211734733
120	42	0.3155370805176787
120	43	0.36280920828571084
120	44	0.15567771552285592
120	45	0.28559014799149196
120	46	0.03771097300896997
120	47	0.16104445846035276
120	48	0.19406690683744188
120	49	0.29451116191174975
120	50	-0.04405666279212994
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	46567.0
3	2601.0
4	2629.0
5	1515.0
6	2052.0
7	2986.0
8	4427.0
9	5991.0
10	8910.0
11	11736.0
12	12676.0
13	18668.0
14	26350.0
15	24618.0
16	21921.0
17	25569.0
18	31616.0
19	41502.0
20	55360.0
21	69762.0
22	109674.0
23	136121.0
24	115360.0
25	90016.0
26	101083.0
27	126413.0
28	149175.0
29	162033.0
30	201333.0
31	237063.0
32	292707.0
33	347451.0
34	490292.0
35	708790.0
36	1048483.0
37	1784633.0
38	3431167.0
39	1.6502473E7
40	370889.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.08787268862146	19.254619576899064	12.107972139972986	23.549535594506487
2	17.123060220983003	22.10619667845833	36.34793117889553	24.422811921663147
3	18.16076674411873	26.242604560659494	32.16984237031054	23.426786324911237
4	11.734638232685466	16.06408127094051	38.07251994853972	34.128760547834304
5	12.092709060689346	39.92476191189882	36.13713639003399	11.845392637377849
6	30.77990157392382	37.607866597406286	17.911886371736912	13.700345456932986
7	26.98365468657564	33.7957317505096	22.651108698884357	16.569504864030392
8	28.873280499304094	33.712548203731984	20.644812667759577	16.769358629204344
9	27.220156635006315	12.701835302244241	19.9208824256191	40.15712563713034
10	17.010677769591705	27.721832656125112	34.949412615371976	20.318076958911206
11	35.23379453127086	21.784820956288673	23.531835751119242	19.449548761321232
12	20.822705961489056	26.77687291634609	32.3502380734059	20.050183048758957
13	29.382265232036314	20.629042391546356	26.458247243035093	23.530445133382237
14	20.620139455471374	20.47538099570616	29.86534644724384	29.039133101578624
15	24.06642201736356	28.94806441669439	24.4907915754066	22.49472199053545
16	22.975566287131173	27.290026787846017	26.616453311854936	23.117953613167874
17	20.677692009206574	27.93857769323206	28.145630947789936	23.23809934977143
18	20.901215016939638	27.74347398008447	29.25024127984066	22.10506972313523
19	21.73593682822538	27.226341714967955	29.974948002826867	21.0627734539798
20	23.30830047424166	25.88925344034354	29.72012569096552	21.08232039444928
21	23.389634834966856	25.89448037349979	29.196213254697195	21.51967153683616
22	22.05769442274819	25.764681319869435	29.552552255861247	22.62507200152112
23	21.904115825856184	27.021096975939557	29.394161910853423	21.680625287350836
24	22.473243652696777	26.2276934113928	29.5456408118612	21.753422124049223
25	21.82084232823296	27.0404205288315	29.640987930778394	21.497749212157146
26	21.597028465753105	26.072224803155365	29.509341486098318	22.821405244993215
27	21.25123571518099	26.25906646405208	29.50211935711821	22.987578463648727
28	22.571043218394543	26.115560109441454	29.685211476836788	21.62818519532722
29	21.368873551066507	27.233685151304798	29.74663408315775	21.650807214470944
30	21.463775070379718	26.419933798562788	30.37829067756374	21.738000453493754
31	21.74966906351855	26.923121093258573	29.563252586397894	21.76395725682498
32	21.388261102124297	26.33370108308737	30.677669652848955	21.60036816193938
33	21.442106364093398	26.400667076425812	29.537518863888856	22.619707695591938
34	22.862829786637086	25.84757779104535	29.666112666648765	21.623479755668797
35	21.484375474759506	26.234735532368724	30.72655295733393	21.554336035537837
36	21.82456168663816	27.618125174816683	29.04475752053929	21.512555618005873
37	21.811261321978435	26.204128756143646	30.36951691056294	21.615093011314983
38	22.841206501647	26.80395672402911	28.969742468388798	21.38509430593509
39	21.58461630139144	26.75335378663824	29.067351794614847	22.594678117355475
40	21.610402594759936	26.33142456642431	29.324389670501777	22.733783168313973
41	22.569397996023678	26.529919497954914	28.99135948086114	21.909323025160273
42	21.316392062301666	26.74628156164603	30.11597061162407	21.821355764428237
43	21.69045591110604	26.477256951423072	29.01545939319095	22.816827744279937
44	21.712180978369915	26.717070232963476	29.949343013894055	21.621405774772555
45	21.494641908057226	26.73974031896143	30.01716386574514	21.748453907236208
46	21.778811916918013	26.810256228481315	28.5190544703526	22.891877384248076
47	21.629773551660872	26.975252140992232	29.785578009133758	21.609396298213138
48	22.835264539936773	27.14769320721469	28.02968575398986	21.987356498858677
49	21.838691649706917	26.9329611226724	28.386243663617368	22.84210356400332
50	21.842706680972412	27.05915445878169	29.252738659985344	21.84540020026055
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	47728.0
1	29706.0
2	11684.0
3	28898.0
4	46112.0
5	46826.5
6	47541.0
7	35765.0
8	23989.0
9	33320.0
10	42651.0
11	65008.0
12	87365.0
13	144651.5
14	201938.0
15	199736.5
16	197535.0
17	248823.5
18	300112.0
19	285249.5
20	270387.0
21	298535.0
22	326683.0
23	369059.5
24	411436.0
25	459667.5
26	507899.0
27	580031.5
28	652164.0
29	722153.0
30	792142.0
31	877483.5
32	962825.0
33	1042895.0
34	1122965.0
35	1206352.0
36	1289739.0
37	1352818.0
38	1415897.0
39	1456983.0
40	1498069.0
41	1521529.0
42	1544989.0
43	1572819.0
44	1600649.0
45	1591186.0
46	1581723.0
47	1548516.0
48	1515309.0
49	1489338.5
50	1463368.0
51	1428798.5
52	1394229.0
53	1354581.5
54	1314934.0
55	1252714.0
56	1190494.0
57	1208599.0
58	1226704.0
59	1130774.5
60	1034845.0
61	896490.5
62	758136.0
63	664753.5
64	571371.0
65	503357.5
66	435344.0
67	372991.0
68	310638.0
69	271739.0
70	232840.0
71	194817.5
72	156795.0
73	129656.0
74	102517.0
75	81541.5
76	60566.0
77	47956.0
78	35346.0
79	26869.5
80	18393.0
81	13740.0
82	9087.0
83	6800.0
84	4513.0
85	3115.5
86	1718.0
87	1186.0
88	654.0
89	423.5
90	193.0
91	129.5
92	66.0
93	62.0
94	58.0
95	66.0
96	74.0
97	79.0
98	84.0
99	99.0
100	114.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35257192699950324
2	0.0020467805298007517
3	0.0
4	0.012977856146150123
5	0.001092361922097669
6	0.4312219853905354
7	0.0
8	0.0
9	0.0
10	0.8018943121572203
11	0.0
12	0.7117502202992012
13	0.0
14	0.0
15	0.0
16	0.0
17	1.6404069819896735E-4
18	8.574854678582385E-5
19	0.0
20	0.0
21	0.0
22	9.767877938211236E-4
23	0.0
24	0.013950915742284905
25	1.4912790745360667E-5
26	0.03953380826595113
27	0.026727449213372657
28	0.04039129373380937
29	0.018476947733501867
30	0.015513030572861434
31	0.012176293643586985
32	1.1084975616841493
33	1.117393041363757
34	1.1377117187543107
35	1.8398357326273818
36	1.1258187681348855
37	1.8386762631469298
38	1.8796566121151812
39	1.9001430584016203
40	1.8687814594641268
41	1.1733160066588593
42	1.171295323512863
43	1.8722039449401868
44	1.8739748388411988
45	1.1707099964761074
46	1.131534095188045
47	0.08620338690355735
48	0.06950851766412608
49	1.147830047275038
50	0.0653627618369158
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2.6822612E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.00063832972525
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.43379694578228	21.25923908932384
2	13.214268030396536	8.457300240655407
3	6.114583806236555	5.870117547605124
4	3.5906435921495103	4.596115478532878
5	2.251488698605355	3.6024537773766867
6	1.5630581544937017	3.001131521416845
7	1.0997361964464845	2.463458219641413
8	0.9024365406722634	2.310283628286519
9	0.6597365529089314	1.9000791740284524
>10	3.7012693654447473	21.237536683197668
>50	0.26563213909300354	5.906728793605879
>100	0.1851879027684969	11.197249939109806
>500	0.012624468212732345	2.7448744602343518
>1k	0.0053039175117355975	2.730160455650014
>5k	1.0516017479216662E-4	0.21525642189714028
>10k+	1.285291025141883E-4	2.50801456943796
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	172008	0.6412798276319994	Illumina PCR Primer Index 1 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	101213	0.37734207242754736	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	94946	0.35397745752725346	No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT	87000	0.3243531987115945	Illumina Paired End PCR Primer 2 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59811	0.22298723181769176	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	40784	0.15205081443969737	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32947	0.12283292917184949	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32594	0.12151687538857141	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27453	0.10235021108309661	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.728197686340167E-6	0.0	3.728197686340167E-6	0.0
3	0.0	1.4912790745360669E-5	0.0	3.728197686340167E-6	3.728197686340167E-6
4	0.0	1.8640988431700835E-5	0.0	3.728197686340167E-6	3.728197686340167E-6
5	0.0	3.3553779177061504E-5	0.0	7.456395372680334E-6	3.728197686340167E-6
6	7.456395372680334E-6	3.3553779177061504E-5	0.0	7.456395372680334E-6	3.728197686340167E-6
7	1.4912790745360669E-5	3.3553779177061504E-5	0.0	7.456395372680334E-6	7.456395372680334E-6
8	2.6097383804381168E-5	3.3553779177061504E-5	0.0	7.456395372680334E-6	7.456395372680334E-6
9	2.6097383804381168E-5	3.3553779177061504E-5	0.0	7.456395372680334E-6	7.456395372680334E-6
10	2.6097383804381168E-5	4.1010174549741836E-5	0.0	1.4912790745360669E-5	1.11845930590205E-5
11	4.1010174549741836E-5	4.846656992242217E-5	0.0	1.4912790745360669E-5	1.11845930590205E-5
12	4.1010174549741836E-5	4.846656992242217E-5	0.0	1.8640988431700835E-5	1.8640988431700835E-5
13	4.1010174549741836E-5	5.592296529510251E-5	0.0	2.2369186118041E-5	1.8640988431700835E-5
14	2.4233284961211086E-4	5.592296529510251E-5	0.0	2.2369186118041E-5	2.2369186118041E-5
15	2.4233284961211086E-4	7.829215141314351E-5	0.0	3.3553779177061504E-5	2.2369186118041E-5
16	3.429941871432954E-4	7.829215141314351E-5	0.0	3.3553779177061504E-5	2.2369186118041E-5
17	3.429941871432954E-4	8.574854678582385E-5	0.0	4.1010174549741836E-5	2.2369186118041E-5
18	3.429941871432954E-4	1.0811773290386484E-4	0.0	4.4738372236082E-5	2.2369186118041E-5
19	3.504505825159757E-4	1.1557412827654518E-4	0.0	4.4738372236082E-5	2.2369186118041E-5
20	3.57906977888656E-4	1.1557412827654518E-4	0.0	4.846656992242217E-5	2.2369186118041E-5
21	3.914607570657175E-4	1.3421511670824601E-4	0.0	6.337936066778283E-5	2.6097383804381168E-5
22	3.989171524383979E-4	1.3421511670824601E-4	0.0	6.337936066778283E-5	2.6097383804381168E-5
23	4.0264535012473804E-4	1.3794331439458617E-4	0.0	6.337936066778283E-5	2.9825581490721337E-5
24	4.0264535012473804E-4	1.3794331439458617E-4	0.0	6.710755835412301E-5	2.9825581490721337E-5
25	4.0264535012473804E-4	1.4912790745360668E-4	0.0	8.202034909948367E-5	2.9825581490721337E-5
26	4.324709316154594E-4	1.5285610513994684E-4	0.0	8.9476744472164E-5	2.9825581490721337E-5
27	4.399273269881397E-4	1.5285610513994684E-4	0.0	1.677688958853075E-4	2.9825581490721337E-5
28	4.622965131061807E-4	1.5658430282628703E-4	0.0	2.609738380438117E-4	2.9825581490721337E-5
29	4.622965131061807E-4	1.6031250051262719E-4	0.0	4.883938969105619E-4	3.3553779177061504E-5
30	4.622965131061807E-4	1.6031250051262719E-4	0.0	7.791933164450949E-4	3.3553779177061504E-5
31	5.219476760876234E-4	1.6404069819896735E-4	0.0	0.0011110029105293698	3.3553779177061504E-5
32	5.256758737739635E-4	1.677688958853075E-4	0.0	0.0015024636675950874	3.3553779177061504E-5
33	5.256758737739635E-4	1.677688958853075E-4	0.0	0.002139985471959256	3.728197686340167E-5
34	5.294040714603037E-4	1.7522529125798785E-4	0.0	0.0031838808241345024	4.1010174549741836E-5
35	5.294040714603037E-4	1.7522529125798785E-4	0.0	0.004701257282474951	4.1010174549741836E-5
36	5.294040714603037E-4	1.7522529125798785E-4	0.0	0.007441482581934973	4.4738372236082E-5
37	5.294040714603037E-4	2.0877907043504934E-4	0.0	0.012564026202966363	4.846656992242217E-5
38	5.294040714603037E-4	2.0877907043504934E-4	0.0	0.020404425937339734	4.846656992242217E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	33915	0.0	36.870914	35
CCGTCTT	34415	0.0	36.22091	39
GCCGTCT	34550	0.0	35.989403	38
CGTCTTC	34960	0.0	35.62342	40
ATGCCGT	35420	0.0	35.12261	36
TCGTATG	35510	0.0	35.019547	32
CGTATGC	35835	0.0	34.83933	33
GGTATCA	39975	0.0	34.784687	1
TATCGTA	22695	0.0	34.771786	30
ATATCGT	22720	0.0	34.733124	29
GACCGAT	35410	0.0	34.538097	24
ATCGTAT	23155	0.0	34.50366	31
TGCCGTC	36270	0.0	34.26286	37
GATATCG	23205	0.0	34.1218	28
GCGGGCT	38165	0.0	34.024975	8
TGAGCGG	38315	0.0	33.824894	5
AGACCGA	37055	0.0	33.81558	23
CGATATC	23465	0.0	33.73135	27
ACCGATA	23395	0.0	33.71826	25
CCGATAT	23580	0.0	33.49431	26
>>END_MODULE