SRR522083_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR522083_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	32632855
Sequences flagged as poor quality	0
Sequence length	50
%GC	44

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	133083	0.40781905230173704	No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	127465	0.39060327390907107	Illumina PCR Primer Index 1 (95% over 24bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	127150	0.38963798907573366	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	78698	0.24116185972695312	No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT	70353	0.2155894726342516	Illumina Paired End PCR Primer 2 (96% over 29bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43490	0.13327059492649354	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43186	0.1323390184524155	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36398	0.11153789639306766	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	27620	36.251392	35
CCGTCTT	28510	35.05039	39
GGTATCA	52425	35.010338	1
GCCGTCT	28715	34.80043	38
TCGTATG	28585	34.79139	32
GACCGAT	28125	34.77787	24
ATATCGT	18215	34.212948	29
CGTATGC	29285	34.099056	33
TATCGTA	18330	33.942505	30
ACCGATA	18640	33.758926	25
CGTCTTC	29580	33.729977	40
GCGGGCT	30965	33.597637	8
ATGCCGT	29785	33.594517	36
ATCGTAT	18955	33.56436	31
AGACCGA	30345	33.390446	23
GATATCG	18910	33.22058	28
CGATATC	18930	33.17282	27
TGAGCGG	31480	33.145824	5
TGCCGTC	30350	32.991554	37
CCGATAT	19080	32.843685	26