FastQCFastQC Report
Thu 26 May 2016
SRR522082_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522082_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32031874
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG453700.036.7415935
GCCGTCT460200.035.982438
CCGTCTT460950.035.9288139
ATGCCGT466950.035.4912536
GACCGAT472150.035.2869124
TGCCGTC469800.035.27027537
TCGTATG477900.035.2616832
CGTCTTC476750.035.2524940
ATATCGT303800.035.08192429
CGTATGC475400.035.05421433
TATCGTA306450.034.80792630
GCGGGCT503850.034.789748
GGTATCA493400.034.714551
TGAGCGG507350.034.714285
AGACCGA493100.034.63904623
GATATCG308900.034.60818528
ACCGATA309400.034.54772625
CCGATAT309300.034.50307526
CGATATC309950.034.47699427
ATCGTAT314400.034.46396631
Produced by FastQC (version 0.11.5)