SRR522081_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR522081_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	32005877
Sequences flagged as poor quality	0
Sequence length	50
%GC	44

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	63910	0.19968207713852051	Illumina PCR Primer Index 1 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47325	0.1478634689497807	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45116	0.14096161151903444	No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT	36202	0.11311047655404038	Illumina Paired End PCR Primer 2 (96% over 29bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CTTCTGC	31520	43.766163	43
TTCTGCT	31485	43.713993	44
TCTTCTG	36445	37.76574	42
TATGCCG	40890	35.624416	35
CCGTCTT	41270	35.2432	39
CGTCTTC	41695	35.203007	40
GCCGTCT	41495	35.076496	38
CGTATGC	42890	33.97034	33
TCGTATG	43470	33.92927	32
ATGCCGT	43020	33.892418	36
TGCCGTC	43115	33.793037	37
GACCGAT	43750	33.09156	24
AGACCGA	45500	32.61835	23
GCGGGCT	46670	32.3102	8
ATATCGT	26470	32.272503	29
GGTATCA	54605	32.24235	1
TGAGCGG	46975	32.03785	5
ATCGTAT	27220	31.918694	31
TATCGTA	26835	31.835087	30
ACCGATA	26860	31.834269	25