FastQCFastQC Report
Thu 26 May 2016
SRR522080_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522080_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences663835
Sequences flagged as poor quality0
Sequence length40
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTATACTCNGCGTTGATACCACTGCTTATACTCTGNGT63010.9491816490543583No Hit
GCTTATACTCTGCGTTGATACCACTNCTTATACTCTGCGT61960.933364465567498No Hit
GCTTATACTCNGCGTTGATACCACTGNTTATANTCTGCGT61860.9318580671401779No Hit
GCTTATACTCTGCGTTGATNCCACTNCTTATACTCTGCGT61240.9225183968907936No Hit
GAGTATAAGCNGTGGTATCAACGCAGAGTATAAGCAGNGG45800.6899304797125791No Hit
GAGTATAAGCNGTGGTATCAACGCAGNGTATANGCAGTGG45450.684658085216959No Hit
GAGTATAAGCAGTGGTATCNACGCANAGTATAAGCAGTGG45390.683754246160567No Hit
GAGTATAAGCAGTGGTATCAACGCANAGTATAAGCAGTGG45160.6802895297777309No Hit
GTATAAGCAGTGGTATCAACGCAGANTATAAGCAGTGGTA41660.6275655848215295No Hit
GTATAAGCAGNGGTATCAACGCAGAGTATAAGCAGTGNTA41240.6212387114267853No Hit
GTATAAGCAGNGGTATCAACGCAGAGNATAAGNAGTGGTA40800.6146105583465771No Hit
GTATAAGCAGTGGTATCAANGCAGANTATAAGCAGTGGTA40780.6143092786611131No Hit
GCTTATACTCNGCGTTGATACCACTGCTTGTACTCTGNGT29630.4463458540149284No Hit
GCTTATACTCNGCGTTGATACCACTGNTTGTANTCTGCGT29100.43836194235013215No Hit
GCTTATACTCTGCGTTGATNCCACTNCTTGTACTCTGCGT28400.42781715335889187No Hit
GCTTATACTCTGCGTTGATACCACTNCTTGTACTCTGCGT28360.4272145939879639No Hit
GAGTAAGCAGNGGTATCAACGCAGAGNAAGCANTGGTATC26460.3985930238688831No Hit
GAGTAAGCAGNGGTATCAACGCAGAGTAAGCAGTGGTNTC25300.3811188021119706No Hit
GAGTAAGCAGTGGTATCAACGCAGANTAAGCAGTGGTATC25000.3765996068300105No Hit
GAGTAAGCAGTGGTATCAANGCAGANTAAGCAGTGGTATC24440.36816377563701824No Hit
GAGTACAAGCAGTGGTATCAACGCANAGTATAAGCAGTGG22820.34376012111443355No Hit
GAGTACAAGCNGTGGTATCAACGCAGAGTATAAGCAGNGG22780.34315756174350553No Hit
GAGTACAAGCNGTGGTATCAACGCAGNGTATANGCAGTGG22730.3424043625298455No Hit
GAGTACAAGCAGTGGTATCNACGCANAGTATAAGCAGTGG22370.3369813281914934No Hit
GAGTATAAGCNGTGGTATCAACGCAGNGTACANGCAGTGG21580.32508078061566503No Hit
GAGTATAAGCAGTGGTATCNACGCANAGTACAAGCAGTGG21120.3181513478499928No Hit
GAGTATAAGCAGTGGTATCAACGCANAGTACAAGCAGTGG20700.31182447445524863No Hit
GAGTATAAGCNGTGGTATCAACGCAGAGTACAAGCAGNGG20230.3047444018468444No Hit
GTATAAGCAGTGGTATCAANGCAGANTACAAGCAGTGGTA19380.29194001521462415No Hit
GTATAAGCAGTGGTATCAACGCAGANTACAAGCAGTGGTA19000.28621570119080797No Hit
GTATAAGCAGNGGTATCAACGCAGAGNACAAGNAGTGGTA18190.27401387392951565No Hit
GTATAAGCAGNGGTATCAACGCAGAGTACAAGCAGTGNTA17210.2592511693417792No Hit
GCTTATACTCTGCGTTGATACCACTNCTTACTCTGCGTTG14960.22535720472707824No Hit
GCTTATACTCTGCGTTGATNCCACTNCTTACTCTGCGTTG14710.22159120865877815No Hit
GAGTACAAGCNGTGGTATCAACGCAGAGTACAAGCAGNGG14590.21978353054599412No Hit
GCTTACTCTGNGTTGATACCACTGCTTACTCTGCGTTNAT14440.21752393290501407No Hit
GCTTATACTCNGCGTTGATACCACTGCTTACTCTGCGNTG14430.21737329306228204No Hit
GTACAAGCAGNGGTATCAACGCAGAGNATAAGNAGTGGTA14360.216318814163158No Hit
GCTTACTCTGCGTTGATACCACTGCNTACTCTGCGTTGAT14220.21420985636490994No Hit
GCGTTGATACCACTGCTTANACTCTNCGTTGATACCACTG14050.21164897903846588No Hit
GCTTATACTCNGCGTTGATACCACTGNTTACTNTGCGTTG13980.21059450013934186No Hit
GCTTACTCTGCGTTGATACNACTGCNTACTCTGCGTTGAT13980.21059450013934186No Hit
GAGTACAAGCNGTGGTATCAACGCAGNGTACANGCAGTGG13950.21014258061114585No Hit
GAGTACAAGCAGTGGTATCAACGCANAGTACAAGCAGTGG13940.20999194076841382No Hit
GAGTACAAGCAGTGGTATCNACGCANAGTACAAGCAGTGG13900.20938938139748584No Hit
GTACAAGCAGNGGTATCAACGCAGAGTATAAGCAGTGNTA13850.20863618218382582No Hit
GCGTTGATACCACTGCTTATACTCTNCGTTGATACCACTG13780.2075817032847018No Hit
GCTTACTCTGNGTTGATACCACTGCTNACTCTNCGTTGAT13440.2024599486318136No Hit
GCGTTGATACNACTGCTTATACTCTGNGTTGANACCACTG13180.1985433127207815No Hit
CTGCTTACTCNGCGTTGATACCACTGCTTACTCTGCGNTG13160.19824203303531754No Hit
CTTATACTCTNCGTTGATACCACTGCTTATACTCTGCNTT13090.1971875541361935No Hit
GTACAAGCAGTGGTATCAACGCAGANTATAAGCAGTGGTA13040.19643435492253347No Hit
GTACAAGCAGTGGTATCAANGCAGANTATAAGCAGTGGTA12920.1946266768097494No Hit
CTTATACTCTGCGTTGATACCACTGNTTATACTCTGCGTT12870.1938734775960894No Hit
GCGTTGATACNACTGCTTATACTCTGCGTTGATACCANTG12700.1913126002696453No Hit
CTTATACTCTNCGTTGATACCACTGCNTATACNCTGCGTT12470.18784788388680923No Hit
CTTATACTCTGCGTTGATANCACTGNTTATACTCTGCGTT12120.18257548939118906No Hit
CTGCTTACTCNGCGTTGATACCACTGNTTACTNTGCGTTG12090.18212356986299305No Hit
CTGCTTACTCTGCGTTGATACCACTNCTTACTCTGCGTTG11990.18061717143567302No Hit
CTGCTTACTCTGCGTTGATNCCACTNCTTACTCTGCGTTG11760.17715245505283692No Hit
GAGTATAAGCAGTGGTATCAACGCANAGTAAGCAGTGGTA11330.17067494181536075No Hit
GAGTATAAGCNGTGGTATCAACGCAGAGTAAGCAGTGNTA11280.16992174260170073No Hit
GAGTAAGCAGNGGTATCAACGCAGAGTATAAGCAGTGNTA11080.16690894574706064No Hit
GAGTATAAGCAGTGGTATCNACGCANAGTAAGCAGTGGTA10880.16389614889242055No Hit
GAGTAAGCAGNGGTATCAACGCAGAGNATAAGNAGTGGTA10810.16284166999329652No Hit
GAGTAAGCAGTGGTATCAANGCAGANTATAAGCAGTGGTA10580.15937695361046045No Hit
GCTTGTACTCTGCGTTGATNCCACTNCTTATACTCTGCGT10460.15756927549767638No Hit
GAGTAAGCAGTGGTATCAACGCAGANTATAAGCAGTGGTA10460.15756927549767638No Hit
GAGTATAAGCNGTGGTATCAACGCAGNGTAAGNAGTGGTA10360.15606287707035635No Hit
CTGCTTATACTCTGCGTTGNTACCANTGCTTATACTCTGC10270.15470711848576832No Hit
GTATAAGCAGNGGTATCAACGCAGAGNAAGCANTGGTATC10150.15289944037298425No Hit
GTATAAGCAGTGGTATCAACGCAGANTAAGCAGTGGTATC10150.15289944037298425No Hit
GTATAAGCAGNGGTATCAACGCAGAGTAAGCAGTGGTNTC10130.15259816068752025No Hit
GCGTTGATACCACTGCTTANTCTGCNTTGATACCACTGCT10010.1507904825747362No Hit
GTACAAGCAGTGGTATCAACGCAGANTACAAGCAGTGGTA9970.15018792320380817No Hit
CTGCTTATACTCTGCGTTGATACCANTGCTTATACTCTGC9960.15003728336107616No Hit
CTGCTTATACNCTGCGTTGATACCACTGCTTATACTCNGC9920.14943472399014815No Hit
GTATAAGCAGTGGTATCAANGCAGANTAAGCAGTGGTATC9920.14943472399014815No Hit
GCGTTGATACNACTGCTTACTCTGCGNTGATANCACTGCT9780.14732576619190008No Hit
GTACAAGCAGNGGTATCAACGCAGAGTACAAGCAGTGNTA9610.14476488886545602No Hit
GCGTTGATACNACTGCTTACTCTGCGTTGATACCACTNCT9490.14295721075267198No Hit
CTGCTTATACNCTGCGTTGATACCACNGCTTANACTCTGC9240.13919121468437187No Hit
GCGTTGATACCACTGCTTACTCTGCNTTGATACCACTGCT9170.13813673578524785No Hit
GCTTGTACTCNGCGTTGATACCACTGCTTATACTCTGNGT9120.13738353657158783No Hit
GCTTGTACTCNGCGTTGATACCACTGNTTATANTCTGCGT9090.1369316170433918No Hit
GTACAAGCAGNGGTATCAACGCAGAGNACAAGNAGTGGTA8930.13452137955967974No Hit
GCTTGTACTCTGCGTTGATACCACTNCTTATACTCTGCGT8820.13286434128962768No Hit
GTACAAGCAGTGGTATCAANGCAGANTACAAGCAGTGGTA8560.12894770537859557No Hit
GTGGTATCAACGCAGAGTANAAGCANTGGTATCAACGCAG8180.12322339135477942No Hit
GGTATCAACGNAGAGTATAAGCAGTGNTATCANCGCAGAG7940.11960803512921132No Hit
GGTATCAACGCAGAGTATANGCAGTNGTATCAACGCAGAG7870.11855355623008729No Hit
GGTATCAACGNAGAGTATAAGCAGTGGTATCAACGCANAG7830.11795099685915927No Hit
GTATCAACGCAGAGTATAAGCAGTGNTATCAACGCAGAGT7570.11403436094812718No Hit
GTATCAACGCNGAGTATAAGCAGTGGNATCAANGCAGAGT7500.11297988204900314No Hit
GCTTACTCTGNGTTGATACCACTGCTTATACTCTGCGNTG7470.11252796252080714No Hit
GTATCAACGCAGAGTATAANCAGTGNTATCAACGCAGAGT7450.11222668283534312No Hit
GGTATCAACGCAGAGTATAAGCAGTNGTATCAACGCAGAG7440.11207604299261112No Hit
GCTTACTCTGCGTTGATACCACTGCNTATACTCTGCGTTG7280.10966580550889905No Hit
GCTTACTCTGNGTTGATACCACTGCTNATACTNTGCGTTG7130.10740620786791899No Hit
GTATCAACGCNGAGTATAAGCAGTGGTATCAACGCAGNGT7040.10605044928333095No Hit
GCTTACTCTGCGTTGATACNACTGCNTATACTCTGCGTTG6810.10258573290049486No Hit
TATCAACGCAGAGTATAAGNAGTGGNATCAACGCAGAGTA6730.10138061415863882No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGCT359.2558126E-538.83672722
CCACCGC359.2558126E-538.83672721
CACCCTG402.0526754E-433.91860633
GTATCGC250.00627672228.8020846
GTGCATT507.5878354E-427.1348869
ACCACCG509.827447E-625.63420120
ACCGCTT550.001308905724.71428123
TCACCCT550.001323262924.66807732
GTGGGAT550.001323262924.66807733
AGTGGGA550.001323262924.66807732
CCACTAC801.751337E-523.78749721
CACTACT801.751337E-523.78749722
AGAGAAA701.941054E-423.30203623
AGAGCAT600.002171835422.65475825
TGTGCAT600.002195593722.6124048
ACCACTA1006.039045E-1021.97217220
CGGAGTA1155.599268E-721.27577222
ACTACTT904.3497144E-521.1444423
GCAGGGG1051.1277734E-920.90214534
TCTGCGA650.003493828720.8729889
Produced by FastQC (version 0.11.5)