FastQCFastQC Report
Thu 26 May 2016
SRR522079_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522079_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1089998
Sequences flagged as poor quality0
Sequence length40
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGNGGTATCAACGCAGAGNAAGCANTGGTATC40660.37302820739120623No Hit
GGTATCAACGNAGAGTACTTTTTTTTNTTTTTNTTTTTTT23530.21587195572835913No Hit
GTATCAACGCNGAGTACTTTTTTTTTNTTTTTNTTTTTTT21340.19578017574344173No Hit
GAGTATAAGCNGTGGTATCAACGCAGNGTATANGCAGTGG17570.16119295631735103No Hit
GCTTATACTCNGCGTTGATACCACTGNTTATANTCTGCGT17200.15779845467606363No Hit
TATCAACGCANAGTACTTTTTTTTTTNTTTTTNTTTTTTT16920.15522964262319747No Hit
GTATAAGCAGNGGTATCAACGCAGAGNATAAGNAGTGGTA16170.14834889605302026No Hit
CTGCTTACTCNGCGTTGATACCACTGNTTACTNTGCGTTG12700.11651397525500047No Hit
GCTTACTCTGNGTTGATACCACTGCTNACTCTNCGTTGAT11540.1058717538931264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGCCG404.9283444E-639.919779
AGGCCGC251.3912214E-436.2487076
GGCGGGA701.3887984E-731.95081324
GGGCGGG752.5429836E-729.8207623
AGGGCGG857.5968273E-726.31243522
CGGGACA758.565063E-626.09316626
GAGCTCG600.002460294422.1776499
GCGGGAC903.51201E-521.74430525
TGTCCCG5450.020.5092411
GCTCTCG650.003913815620.47167610
AGCTCGA650.003913815620.47167610
TAGGGCG1107.1382656E-620.33233621
CACGCAG450.00430641320.138175
AGACCCG450.00430641320.138175
ACGGGGC4200.019.9692623
GGGGCTG4200.019.9692625
ATCGAGC5350.019.85728326
CGGGGCT4300.019.50485824
GTAGGGC951.6510967E-719.49833520
CTCACAA2900.019.27161232
Produced by FastQC (version 0.11.5)