##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522079_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1089998
Sequences flagged as poor quality	0
Sequence length	40
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0189137961721	31.0	31.0	34.0	31.0	34.0
2	32.31969416457645	33.0	31.0	34.0	31.0	34.0
3	32.51596149717706	34.0	31.0	34.0	31.0	34.0
4	35.900243853658445	37.0	35.0	37.0	35.0	37.0
5	35.65587276306929	37.0	35.0	37.0	35.0	37.0
6	36.0239073833163	37.0	35.0	37.0	35.0	37.0
7	36.073091877232805	37.0	35.0	37.0	35.0	37.0
8	36.20782239967413	37.0	35.0	37.0	35.0	37.0
9	38.03954686155387	39.0	38.0	39.0	35.0	39.0
10	38.029237668326	39.0	38.0	39.0	35.0	39.0
11	23.7420775083991	37.0	2.0	39.0	2.0	39.0
12	29.808465703606796	37.0	17.0	39.0	17.0	39.0
13	35.20417285169331	37.0	32.0	39.0	32.0	39.0
14	30.64793605125881	37.0	32.0	40.0	2.0	41.0
15	34.475512799106056	39.0	34.0	40.0	17.0	41.0
16	37.37682637949794	39.0	36.0	40.0	32.0	41.0
17	38.586623094721276	40.0	37.0	41.0	35.0	41.0
18	38.876001607342396	40.0	38.0	41.0	36.0	41.0
19	39.190918698933395	40.0	39.0	41.0	36.0	41.0
20	31.852796977609135	40.0	35.0	41.0	2.0	41.0
21	34.85907772307839	40.0	34.0	41.0	18.0	41.0
22	37.65757093132281	40.0	35.0	41.0	32.0	41.0
23	38.66923609034145	40.0	37.0	41.0	35.0	41.0
24	38.92178792988611	40.0	38.0	41.0	36.0	41.0
25	38.97894216319663	40.0	38.0	41.0	36.0	41.0
26	23.914302595050632	37.0	2.0	40.0	2.0	41.0
27	26.91870076825829	37.0	16.0	40.0	2.0	41.0
28	36.84929054915697	40.0	34.0	41.0	30.0	41.0
29	38.036000066055166	40.0	37.0	41.0	34.0	41.0
30	38.348306143681	40.0	38.0	41.0	35.0	41.0
31	38.48958163225988	40.0	38.0	41.0	35.0	41.0
32	38.324327200600365	40.0	38.0	41.0	34.0	41.0
33	31.21309855614414	39.0	33.0	41.0	2.0	41.0
34	38.312570298294126	40.0	38.0	41.0	34.0	41.0
35	38.26741792186775	40.0	38.0	41.0	34.0	41.0
36	38.04325329037301	40.0	38.0	41.0	34.0	41.0
37	30.906709003135784	39.0	31.0	41.0	2.0	41.0
38	26.224983899053026	35.0	15.0	40.0	2.0	41.0
39	31.038046858801575	35.0	23.0	40.0	15.0	41.0
40	34.68220308661117	37.0	30.0	40.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	0.15759110977434432
1	2	0.03759748826990972
1	3	0.10616352256710115
1	4	0.06656313688726101
1	5	0.15163636735285735
1	6	0.03856650989352062
1	7	0.06162254851381732
1	8	0.021964232120126326
1	9	0.016546741274765964
1	10	0.02081039729881695
1	11	-21.640829938808096
1	12	-12.39637528360122
1	13	-4.294208043130379
1	14	5.426803267035687
1	15	3.317977393979355
1	16	1.3006928248342007
1	17	0.6480723582007073
1	18	0.34570288719955045
1	19	0.17025725532938196
1	20	7.573966814318858
1	21	4.435685045651091
1	22	1.798545480502419
1	23	0.8249569587730221
1	24	0.6012750465401098
1	25	0.3970777479088028
1	26	15.016971339762176
1	27	-25.08512090148151
1	28	2.1270124407917663
1	29	0.8027036395436014
1	30	0.41357451841004433
1	31	0.18714797292587804
1	32	0.07259483749330542
1	33	-29.162007481700414
1	34	0.00262299047771819
1	35	-0.08586016363721427
1	36	0.029657254487489126
1	37	7.314566550013787
1	38	11.88605202538529
1	39	6.842444784280151
1	40	3.013994666998677
3	1	-0.09315441981821593
3	2	0.0219216970685423
3	3	0.06135502454946362
3	4	-0.12844864453247595
3	5	-0.3204775112436309
3	6	-0.14650480284635137
3	7	-0.07631698516988195
3	8	-0.09710149661984246
3	9	-0.07514693567565445
3	10	-0.0072485992723372306
3	11	14.422102530316891
3	12	8.281025987930011
3	13	2.904948259188693
3	14	9.056543186938313
3	15	5.224289149241336
3	16	2.1826648402841755
3	17	0.8463987085258751
3	18	0.10558953312454378
3	19	0.16203522794900493
3	20	7.5189967374340085
3	21	4.099732295127318
3	22	1.6663617223306844
3	23	0.5855129707012665
3	24	0.39325061942384565
3	25	-0.08428964043722686
3	26	-22.29147006883154
3	27	-10.924176695472926
3	28	-8.424408058911315
3	29	-3.5277352018329537
3	30	-1.772358494038052
3	31	-0.7778910244528845
3	32	-0.31022720092549605
3	33	7.198595128442356
3	34	0.0360692287381994
3	35	0.05152892608562354
3	36	0.196459159369752
3	37	6.537392823913613
3	38	11.607357410548332
3	39	6.682671856092792
3	40	3.0316287830217377
5	1	-0.07597781419321237
5	2	0.17903738004032022
5	3	0.12477973503622763
5	4	0.06429599738440572
5	5	0.09279530459446761
5	6	0.0419255384272077
5	7	0.06820661858838406
5	8	0.05643234597474134
5	9	0.07613166031158869
5	10	0.1124795273418755
5	11	14.495757877430833
5	12	8.272416657913677
5	13	2.8857006519800947
5	14	9.20221361927306
5	15	5.364594044736769
5	16	2.1189059744498664
5	17	0.9419069842005285
5	18	0.6866625713750238
5	19	0.32619370425245364
5	20	-29.871780174189013
5	21	-17.31869782143475
5	22	-7.092885233542578
5	23	-2.8043015653611434
5	24	-1.3031264649542322
5	25	-0.6267964149607153
5	26	-22.29147006883154
5	27	12.03009509997981
5	28	2.1794888031379003
5	29	1.0051273658587832
5	30	0.5990786858645691
5	31	0.45588952368044033
5	32	0.40726009213563685
5	33	7.51280300327538
5	34	0.14288580023553976
5	35	0.13625447003762758
5	36	-0.1348911023839321
5	37	7.37352571657776
5	38	11.895888717598922
5	39	6.9473781692529535
5	40	3.2207936844819116
6	1	0.08023334946180682
6	2	-0.23384930449318375
6	3	-0.043861396437897326
6	4	0.09208398756484115
6	5	0.23646262060244538
6	6	0.1649657329772083
6	7	-0.01974352649289557
6	8	0.05690830720291018
6	9	0.02334415776262233
6	10	-0.04576140047760191
6	11	-21.640829938808096
6	12	-12.375583407719848
6	13	-4.194250541668129
6	14	4.654147944159021
6	15	3.099296935700096
6	16	1.188299171412993
6	17	0.5450072157941506
6	18	0.5184307592157538
6	19	0.15931067041316993
6	20	7.649308198333742
6	21	4.429969472148294
6	22	1.928781776009636
6	23	0.7997762729336628
6	24	-0.13281372055176632
6	25	-0.03736575828066435
6	26	14.78188882616546
6	27	11.956224319565287
6	28	2.110210466961547
6	29	0.9672387020034918
6	30	0.530160781389931
6	31	0.06379083785432726
6	32	-0.20051019969092465
6	33	7.227469120138181
6	34	-0.11752732781550179
6	35	-0.036262161887421485
6	36	-0.025112019925742857
6	37	7.411949097931235
6	38	-24.008303446068993
6	39	-16.396263322855393
6	40	-7.807645974586077
11	1	-0.06869222522470508
11	2	-0.004707260885595588
11	3	-0.2484368857149093
11	4	-0.09449447730406746
11	5	-0.16041678130613946
11	6	-0.09895297845157813
11	7	-0.03376865543942387
11	8	-0.038203388677956696
11	9	-0.040875623673294115
11	10	-0.08027992489076041
11	11	14.363799469868468
11	12	8.218516045477397
11	13	2.697809673629685
11	14	-28.33970801740609
11	15	-17.006157523657606
11	16	-6.790562810981239
11	17	-2.981385266721283
11	18	-1.656385750914886
11	19	-0.8177968579440389
11	20	7.129508424102404
11	21	4.353311008508037
11	22	1.6991962546998565
11	23	0.5940553629531706
11	24	0.44141451954205024
11	25	0.3513740657698392
11	26	14.78407997173544
11	27	12.022978177409342
11	28	2.0076963480200973
11	29	0.7526654944270632
11	30	0.2295445083735146
11	31	0.07106268999222465
11	32	0.030882470987478428
11	33	7.223140229844475
11	34	-0.06405069163598398
11	35	-0.0656610705986509
11	36	-0.0661132915475875
11	37	-28.637434188436394
11	38	-11.380994707463543
11	39	-4.07623148677051
11	40	-1.458771159916239
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	0.0
11	1.0
12	0.0
13	0.0
14	2.0
15	7.0
16	14.0
17	10.0
18	26.0
19	47.0
20	82.0
21	177.0
22	383.0
23	751.0
24	1277.0
25	2323.0
26	4089.0
27	6736.0
28	10622.0
29	16950.0
30	24485.0
31	36222.0
32	55022.0
33	94322.0
34	191429.0
35	380134.0
36	173473.0
37	91413.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.663670530202374	18.74224662521746	10.52045789871615	20.07362494586401
2	15.859720328735754	24.606465723664535	37.04359840512883	22.490215542470875
3	25.322362517684756	26.639704492615067	28.84298142435881	19.194951565341373
4	11.266167460857726	16.19379118126822	42.07530656019552	30.464734797678528
5	8.443409987908234	43.91732828867576	35.37823005179826	12.261031671617745
6	25.019311962040298	37.9037392729161	21.238938053097346	15.83801071194626
7	25.383532813821674	35.85969882513546	21.1749929816385	17.581775379404366
8	23.630502074315732	31.203635236027953	20.99682751711471	24.1690351725416
9	24.335732726115094	18.415079660696627	24.157567261591307	33.09162035159697
10	22.23756373864906	24.675916836544655	28.572070774441787	24.514448650364496
11	28.929107533789853	23.09097607687553	27.0227478252397	20.957168564094914
12	29.34818109424255	25.494772457880575	27.74395502368816	17.41309142418871
13	31.538223005913775	18.58296987700895	27.469866917187	22.408940199890274
14	22.663086748282446	21.424993781709027	33.87475055851043	22.037168911498103
15	26.055001935783366	28.747942656775514	26.669131502993583	18.527923904447533
16	17.17425169587467	26.88665483789878	35.21052332206114	20.7285701441654
17	21.26664452595326	28.803355602487347	27.251609635980984	22.678390235578412
18	17.605078174455365	29.740605028633084	32.10382037398234	20.550496422929214
19	18.238657318637284	31.343727236196763	30.475927478766017	19.941687966399936
20	19.573760667177183	26.521073738279448	28.308073473071012	25.597092121472354
21	23.57444692559069	26.533167950766885	29.368310767542695	20.524074356099735
22	19.54884320888662	23.038115666267277	27.77096838709796	29.642072737748144
23	20.99948807245518	30.287028049592752	29.82143086501076	18.89205301294131
24	26.17151591103837	22.915363147455317	28.086290066587278	22.826830874919036
25	18.93201344960298	30.02977077876504	31.17262005789017	19.865595713741808
26	29.638931030458632	23.154160345431205	29.518808106741357	17.68810051736881
27	18.232414963571358	23.605324697038757	36.81514497019058	21.3471153691993
28	20.232441006718364	28.870721662536685	32.5395390996489	18.35729823109605
29	19.478402234134588	26.437320469689368	33.30642194428058	20.777855351895465
30	19.341059383922087	31.788857165924156	31.216375900006604	17.653707550147157
31	20.329799375410552	25.27165237303623	30.27690009871596	24.121648152837256
32	19.138643165274896	29.03902647576373	30.845576699713206	20.976753659248168
33	21.577398847174457	24.57708160827524	30.565108989313515	23.280410555236788
34	19.27057672917372	24.845200315241794	32.94735480390511	22.93686815167938
35	24.867562704115855	24.684586803895535	29.539321007083203	20.908529484905404
36	22.56169277374821	24.689494843109806	31.628865374064908	21.11994700907708
37	24.45861116884562	24.85839900870788	32.5032056851648	18.179784137281693
38	24.196801861001845	24.8032072974718	30.769151513208808	20.230839328317543
39	21.599489173374632	24.209769192237047	35.14455989827504	19.046181736113276
40	22.06143686634	26.768605785153536	30.755680978938475	20.41427636956799
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1237.0
1	910.0
2	583.0
3	583.0
4	2034.0
5	3485.0
6	3297.0
7	3109.0
8	3109.0
9	2781.5
10	2454.0
11	3983.5
12	5513.0
13	5513.0
14	8711.5
15	11910.0
16	15032.0
17	18154.0
18	18154.0
19	22814.0
20	27474.0
21	27851.0
22	28228.0
23	28228.0
24	27524.5
25	26821.0
26	29774.0
27	32727.0
28	32727.0
29	36071.0
30	39415.0
31	42595.0
32	45775.0
33	45775.0
34	48252.5
35	50730.0
36	54938.0
37	59146.0
38	59146.0
39	74735.0
40	90324.0
41	108211.0
42	126098.0
43	126098.0
44	132066.0
45	138034.0
46	125099.5
47	112165.0
48	112165.0
49	92598.0
50	73031.0
51	65348.5
52	57666.0
53	57666.0
54	52789.0
55	47912.0
56	41681.5
57	35451.0
58	35451.0
59	29260.5
60	23070.0
61	18519.0
62	13968.0
63	13968.0
64	10700.0
65	7432.0
66	5877.5
67	4323.0
68	4323.0
69	3261.0
70	2199.0
71	1653.5
72	1108.0
73	1108.0
74	685.0
75	262.0
76	181.5
77	101.0
78	101.0
79	71.5
80	42.0
81	34.5
82	27.0
83	27.0
84	21.5
85	16.0
86	8.5
87	1.0
88	1.0
89	3.5
90	6.0
91	3.5
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	0.013761493140354386
2	3.669731504094503E-4
3	0.006972489857779556
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	39.73805456523773
12	1.8348657520472514E-4
13	0.0
14	19.22306279461063
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	20.090403835603368
21	0.0
22	0.0
23	0.0
24	0.0
25	2.752298628070877E-4
26	41.038882640151634
27	19.88847685959057
28	9.174328760236257E-5
29	9.174328760236257E-5
30	5.504597256141754E-4
31	1.8348657520472514E-4
32	0.0018348657520472515
33	19.8935227404087
34	0.0037614747916968655
35	0.02174315916175993
36	0.0
37	19.22306279461063
38	19.861504333035473
39	0.0
40	9.174328760236257E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	1089998.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.23374557171573
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.54160489749783	70.05175135633942
2	7.578570198245918	11.857998653739168
3	1.6829550003249123	3.9499161991219784
4	0.5447828138065771	1.7048160018874856
5	0.22270785464221532	0.8711634818450862
6	0.11693518711786433	0.548896660641598
7	0.06552678778936422	0.35884842308314513
8	0.03958117445330534	0.24772668252876678
9	0.027450865866856414	0.19328256503558466
>10	0.1381699991843302	2.0394538442486367
>50	0.017851278803642543	0.9615736819426427
>100	0.019166315839138307	3.0843153533182686
>500	0.002818575856795176	1.3642079247355177
>1k	0.001879050571196784	2.766049171532705
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGNGGTATCAACGCAGAGNAAGCANTGGTATC	4066	0.37302820739120623	No Hit
GGTATCAACGNAGAGTACTTTTTTTTNTTTTTNTTTTTTT	2353	0.21587195572835913	No Hit
GTATCAACGCNGAGTACTTTTTTTTTNTTTTTNTTTTTTT	2134	0.19578017574344173	No Hit
GAGTATAAGCNGTGGTATCAACGCAGNGTATANGCAGTGG	1757	0.16119295631735103	No Hit
GCTTATACTCNGCGTTGATACCACTGNTTATANTCTGCGT	1720	0.15779845467606363	No Hit
TATCAACGCANAGTACTTTTTTTTTTNTTTTTNTTTTTTT	1692	0.15522964262319747	No Hit
GTATAAGCAGNGGTATCAACGCAGAGNATAAGNAGTGGTA	1617	0.14834889605302026	No Hit
CTGCTTACTCNGCGTTGATACCACTGNTTACTNTGCGTTG	1270	0.11651397525500047	No Hit
GCTTACTCTGNGTTGATACCACTGCTNACTCTNCGTTGAT	1154	0.1058717538931264	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.5871643801181287E-4	0.0
3	0.0	0.0	0.0	4.5871643801181287E-4	0.0
4	0.0	0.0	0.0	5.504597256141754E-4	0.0
5	0.0	0.0	0.0	5.504597256141754E-4	0.0
6	9.174328760236257E-5	0.0	0.0	5.504597256141754E-4	0.0
7	9.174328760236257E-5	0.0	0.0	5.504597256141754E-4	0.0
8	9.174328760236257E-5	0.0	0.0	5.504597256141754E-4	0.0
9	9.174328760236257E-5	0.0	0.0	5.504597256141754E-4	0.0
10	9.174328760236257E-5	0.0	0.0	6.42203013216538E-4	0.0
11	9.174328760236257E-5	0.0	0.0	6.42203013216538E-4	0.0
12	9.174328760236257E-5	0.0	0.0	6.42203013216538E-4	0.0
13	1.8348657520472514E-4	0.0	0.0	0.0010091761636259883	0.0
14	0.0010091761636259883	0.0	0.0	0.0011926627388307136	0.0
15	0.0011009194512283508	0.0	0.0	0.0011926627388307136	0.0
16	0.0011009194512283508	0.0	0.0	0.0011926627388307136	0.0
17	0.0011009194512283508	0.0	0.0	0.0014678926016378011	0.0
18	0.0011009194512283508	0.0	0.0	0.001743122464444889	0.0
19	0.0011926627388307136	0.0	0.0	0.002293582190059064	0.0
20	0.0011926627388307136	0.0	0.0	0.002568812052866152	0.0
21	0.0011926627388307136	0.0	0.0	0.003853218079299228	0.0
22	0.0011926627388307136	0.0	0.0	0.004495421092515766	0.0
23	0.0011926627388307136	0.0	0.0	0.0059633136941535676	0.0
24	0.0011926627388307136	0.0	0.0	0.008623869034622082	0.0
25	0.0011926627388307136	0.0	0.0	0.01036699149906697	0.0
26	0.001284406026433076	0.0	0.0	0.010825707937078783	0.0
27	0.0014678926016378011	0.0	0.0	0.011834884100704771	0.0
28	0.0014678926016378011	0.0	0.0	0.015045899166787462	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGCCG	40	4.9283444E-6	39.91977	9
AGGCCGC	25	1.3912214E-4	36.248707	6
GGCGGGA	70	1.3887984E-7	31.950813	24
GGGCGGG	75	2.5429836E-7	29.82076	23
AGGGCGG	85	7.5968273E-7	26.312435	22
CGGGACA	75	8.565063E-6	26.093166	26
GAGCTCG	60	0.0024602944	22.177649	9
GCGGGAC	90	3.51201E-5	21.744305	25
TGTCCCG	545	0.0	20.50924	11
GCTCTCG	65	0.0039138156	20.471676	10
AGCTCGA	65	0.0039138156	20.471676	10
TAGGGCG	110	7.1382656E-6	20.332336	21
CACGCAG	45	0.004306413	20.13817	5
AGACCCG	45	0.004306413	20.13817	5
ACGGGGC	420	0.0	19.96926	23
GGGGCTG	420	0.0	19.96926	25
ATCGAGC	535	0.0	19.857283	26
CGGGGCT	430	0.0	19.504858	24
GTAGGGC	95	1.6510967E-7	19.498335	20
CTCACAA	290	0.0	19.271612	32
>>END_MODULE