FastQCFastQC Report
Thu 26 May 2016
SRR522078_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522078_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29233996
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT2323260.7947117458728529Illumina PCR Primer Index 1 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT817490.2796367626238986No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT814370.2785695120160788Illumina Paired End PCR Primer 2 (96% over 29bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT697210.23849288342243738No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT422380.14448247170862305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT428000.034.6580438
CCGTCTT428400.034.59981539
TATGCCG431600.034.3116335
CGTCTTC433150.034.18124840
ATGCCGT444900.033.24212636
GGTATCA380600.032.816571
TGCCGTC452550.032.70456737
GACCGAT452700.032.545824
ACCGATA331550.032.16947625
CGTATGC461900.032.0533333
ATCGTAT333650.031.96907631
TCGTATG461450.031.94361332
ATATCGT332150.031.9146729
AGACCGA468300.031.91219323
CCGATAT333850.031.82296626
TATCGTA333200.031.80716930
CGATATC335700.031.73967227
GATATCG337100.031.62743228
GCGGGCT491200.031.5326398
TGAGCGG499000.031.1235985
Produced by FastQC (version 0.11.5)