SRR522077_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR522077_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31123064
Sequences flagged as poor quality	0
Sequence length	50
%GC	46

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	499358	1.6044628510868981	Illumina PCR Primer Index 1 (95% over 24bp)
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT	90716	0.2914751581014003	Illumina Paired End PCR Primer 2 (96% over 29bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	64127	0.20604333814948297	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	56933	0.1829286473851032	No Hit
CTTATACACATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCC	46148	0.14827588954609353	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35456	0.11392194547426308	No Hit
CATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTG	35035	0.1125692508938066	Illumina Paired End PCR Primer 2 (96% over 26bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GCCGTCT	78340	35.943027	38
CCGTCTT	78995	35.583622	39
TATGCCG	80875	35.23822	35
CGTCTTC	79690	35.148594	40
ATGCCGT	81460	34.643635	36
TGCCGTC	81670	34.490932	37
ACCGATA	70080	33.14427	25
GTCTTCT	84970	33.091812	41
CCGATAT	70215	33.046726	26
TATCGTA	70140	32.987835	30
ATCGTAT	71005	32.95017	31
ATATCGT	70230	32.938713	29
GACCGAT	85420	32.935528	24
AGACCGA	87715	32.836777	23
CGATATC	70830	32.795666	27
GATATCG	70890	32.74545	28
CGTATGC	87195	32.740288	33
TCGTATG	86870	32.728687	32
GCGGGCT	90840	32.6305	8
TGAGCGG	91465	32.508324	5