##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522077_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31123064
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.13839135504139	40.0	39.0	40.0	38.0	40.0
2	39.03431284914622	40.0	39.0	40.0	38.0	40.0
3	39.00027773615091	40.0	39.0	40.0	38.0	40.0
4	39.04039859314623	40.0	39.0	40.0	38.0	40.0
5	39.02610199304284	40.0	39.0	40.0	38.0	40.0
6	39.06494402350617	40.0	39.0	40.0	38.0	40.0
7	39.01896005483265	40.0	39.0	40.0	38.0	40.0
8	39.007135255063574	40.0	39.0	40.0	38.0	40.0
9	39.022573484410145	40.0	39.0	40.0	38.0	40.0
10	38.97179059876624	40.0	39.0	40.0	38.0	40.0
11	39.006498653217434	40.0	39.0	40.0	38.0	40.0
12	38.93855399327007	40.0	39.0	40.0	37.0	40.0
13	38.930148683304445	40.0	39.0	40.0	37.0	40.0
14	38.90325865730958	40.0	39.0	40.0	37.0	40.0
15	38.86521979326971	40.0	39.0	40.0	37.0	40.0
16	38.83552120703797	40.0	39.0	40.0	37.0	40.0
17	38.76784467621825	40.0	39.0	40.0	36.0	40.0
18	38.753283192169	40.0	39.0	40.0	36.0	40.0
19	38.72177591512198	40.0	39.0	40.0	36.0	40.0
20	38.68252614845376	40.0	39.0	40.0	36.0	40.0
21	38.60204506214427	40.0	39.0	40.0	36.0	40.0
22	38.523900860146675	40.0	38.0	40.0	35.0	40.0
23	38.50345881755087	40.0	38.0	40.0	35.0	40.0
24	38.427010560399836	40.0	38.0	40.0	35.0	40.0
25	38.3674796286124	40.0	38.0	40.0	35.0	40.0
26	38.182360451400285	40.0	38.0	40.0	35.0	40.0
27	38.09030087783131	40.0	38.0	40.0	35.0	40.0
28	38.22361998163163	40.0	38.0	40.0	35.0	40.0
29	38.09917908468138	40.0	38.0	40.0	35.0	40.0
30	37.988351789528174	40.0	38.0	40.0	35.0	40.0
31	37.70328682934302	40.0	38.0	40.0	35.0	40.0
32	37.9858670727278	40.0	38.0	40.0	35.0	40.0
33	37.988736873721685	40.0	38.0	40.0	35.0	40.0
34	37.97415283405259	40.0	38.0	40.0	35.0	40.0
35	37.827486651057235	40.0	38.0	40.0	34.0	40.0
36	37.981580444650305	40.0	39.0	40.0	35.0	40.0
37	37.92993424426336	40.0	38.0	40.0	35.0	40.0
38	37.856282209232354	40.0	38.0	40.0	35.0	40.0
39	37.76182081558551	40.0	38.0	40.0	35.0	40.0
40	37.71649009236366	40.0	38.0	40.0	35.0	40.0
41	37.602229651939155	40.0	38.0	40.0	35.0	40.0
42	37.4709232355786	40.0	38.0	40.0	34.0	40.0
43	37.40270138569905	40.0	38.0	40.0	33.0	40.0
44	37.29733534590296	40.0	38.0	40.0	33.0	40.0
45	37.19498571862976	40.0	38.0	40.0	33.0	40.0
46	36.966998397072985	40.0	38.0	40.0	33.0	40.0
47	36.88481301198365	40.0	38.0	40.0	33.0	40.0
48	36.758810700643096	39.0	38.0	40.0	33.0	40.0
49	36.62200729979542	39.0	37.0	40.0	32.0	40.0
50	36.60502950480711	39.0	38.0	40.0	32.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1	1	-0.1945116778690661
1	2	-0.230435984093603
1	3	-0.2367043737187302
1	4	-0.21505924475712135
1	5	-0.24676085730309438
1	6	-0.23793630153241452
1	7	-0.259440365715669
1	8	-0.29745314377201026
1	9	-0.26202264529116803
1	10	-0.27877843012309
1	11	-0.268895494765367
1	12	-0.31974193505315185
1	13	-0.31306227509033846
1	14	-0.32393168444451703
1	15	-0.30960092257329563
1	16	-0.3272028087613137
1	17	-0.31851214771131
1	18	-0.3330383100437544
1	19	-0.3707889731258689
1	20	-0.3860306304498451
1	21	-0.4369982345658556
1	22	-0.40445994280043607
1	23	-0.4077920754258102
1	24	-0.4383246635797704
1	25	-0.44812836812233314
1	26	-0.47364928932146455
1	27	-0.5043822791359602
1	28	-0.3668550056145108
1	29	-0.39388605020536716
1	30	-0.41496603233566987
1	31	-0.21345760429581162
1	32	-0.3003239571447125
1	33	-0.34290068790635075
1	34	-0.3590488683766395
1	35	-0.32548228852355265
1	36	-0.3344304560243856
1	37	-0.3731542245509303
1	38	-0.3802639087249844
1	39	-0.34127682502462164
1	40	-0.3871442499103068
1	41	-0.4639984330089604
1	42	-0.4204128462865384
1	43	-0.4857632315448299
1	44	-0.532995135312369
1	45	-0.5026554442024036
1	46	-0.5604366827376595
1	47	-0.558792195125406
1	48	-0.6172619365352077
1	49	-0.6953466245340891
1	50	-0.6717400704849581
2	1	-0.18599850054900458
2	2	-0.22020397823295923
2	3	-0.20926005243958912
2	4	-0.22528230129313442
2	5	-0.24251989969666
2	6	-0.23790244788732196
2	7	-0.23791011998004308
2	8	-0.2502821907330528
2	9	-0.21800834681710768
2	10	-0.2704849910047926
2	11	-0.2864852824740254
2	12	-0.27118731599330204
2	13	-0.27829371623783317
2	14	-0.27138330372751795
2	15	-0.28682779230175015
2	16	-0.3028387835936215
2	17	-0.40079254099197925
2	18	-0.38399338143008777
2	19	-0.3425231500272048
2	20	-0.3689923224532734
2	21	-0.35678595802042423
2	22	-0.40459093652205524
2	23	-0.42225178080023795
2	24	-0.41109485931575307
2	25	-0.46220864858591426
2	26	-0.46409673941182916
2	27	-0.48902779150026277
2	28	-0.40137596050744406
2	29	-0.5324421528982413
2	30	-0.4266458761654732
2	31	-0.33055821456711243
2	32	-0.395360639735884
2	33	-0.37872048751590626
2	34	-0.4202355420969539
2	35	-0.45904231865174694
2	36	-0.5042303951277347
2	37	-0.43088383155765797
2	38	-0.47461559808262876
2	39	-0.4955690619024864
2	40	-0.5163430266506168
2	41	-0.5636232294353647
2	42	-0.4981373114668699
2	43	-0.6418980742830911
2	44	-0.5689835037011832
2	45	-0.7215556443919766
2	46	-0.6753942480981081
2	47	-0.7364981810264553
2	48	-0.767645558123327
2	49	-0.7284587938714537
2	50	-0.7721966138244625
3	1	-0.17980793409692808
3	2	-0.20448169334214583
3	3	-0.19108953499868164
3	4	-0.18034010972809966
3	5	-0.20394477569207936
3	6	-0.21760412067575174
3	7	-0.22519059724100998
3	8	-0.24190956347001702
3	9	-0.2723412164645822
3	10	-0.21924148759774198
3	11	-0.25234310235192936
3	12	-0.2570122971155442
3	13	-0.2570725656588877
3	14	-0.2523694254634776
3	15	-0.35782812097743744
3	16	-0.3396776273384816
3	17	-0.3168745374467292
3	18	-0.294286446126101
3	19	-0.3581827663225212
3	20	-0.3130964013041577
3	21	-0.291734968010978
3	22	-0.36207300085234806
3	23	-0.38899756013721287
3	24	-0.41092177462341795
3	25	-0.3950152775855713
3	26	-0.3367579813860857
3	27	-0.37640909419616264
3	28	-0.3711666515146348
3	29	-0.3404117665530393
3	30	-0.3621069575270113
3	31	-0.2655114847873037
3	32	-0.3007086480167871
3	33	-0.29774749634281505
3	34	-0.29599880751564456
3	35	-0.43164786892832296
3	36	-0.33865910141947353
3	37	-0.3562722070580051
3	38	-0.4336347615011249
3	39	-0.3686478063819365
3	40	-0.4756188946312463
3	41	-0.3780453827941628
3	42	-0.3923639388077049
3	43	-0.3787994631385345
3	44	-0.4871533213942243
3	45	-0.459110083232261
3	46	-0.5707165776904617
3	47	-0.5714291210574487
3	48	-0.5039889186377522
3	49	-0.5181539363658416
3	50	-0.5531867694010515
4	1	-0.15042657787527247
4	2	-0.1804798620648569
4	3	-0.18625707450145512
4	4	-0.17245150735485026
4	5	-0.16959830678072052
4	6	-0.18113010680703212
4	7	-0.21884890804147972
4	8	-0.19136603559615395
4	9	-0.2193810064044044
4	10	-0.21897861303565946
4	11	-0.2050721237692983
4	12	-0.2748591368204458
4	13	-0.25418067536578803
4	14	-0.2714499556985359
4	15	-0.24534425933361348
4	16	-0.28258427336825775
4	17	-0.2790934390669335
4	18	-0.29141287772646507
4	19	-0.2714847731819887
4	20	-0.2888604288803336
4	21	-0.2541058539995831
4	22	-0.30382807571223225
4	23	-0.3081824090526908
4	24	-0.33981807131494435
4	25	-0.3549805500612635
4	26	-0.32449940337760097
4	27	-0.36570748190578684
4	28	-0.3459364863090144
4	29	-0.2764498704319891
4	30	-0.36679748970814785
4	31	-0.18116706391963078
4	32	-0.2839679237866761
4	33	-0.23097352458206188
4	34	-0.23294952269799296
4	35	-0.26636832710264713
4	36	-0.29379323237782984
4	37	-0.2674593173691733
4	38	-0.2644322799994967
4	39	-0.3160915584671784
4	40	-0.33352663916780756
4	41	-0.361953969677117
4	42	-0.3730937069922007
4	43	-0.4315243744368473
4	44	-0.40356192929549195
4	45	-0.40044632585168927
4	46	-0.404604471907156
4	47	-0.4538489175797764
4	48	-0.3988703915154588
4	49	-0.42012605568015715
4	50	-0.4246594245918729
5	1	-0.1730596375394029
5	2	-0.19221687204388616
5	3	-0.2029678814266802
5	4	-0.18515040032843189
5	5	-0.22460417995336002
5	6	-0.21483429340042193
5	7	-0.226687433130337
5	8	-0.22387884140349001
5	9	-0.26266875586925664
5	10	-0.2304855093404754
5	11	-0.26393775071729664
5	12	-0.23334414824806515
5	13	-0.2592894189257393
5	14	-0.2987805781254238
5	15	-0.3007288428641317
5	16	-0.27021329480682255
5	17	-0.29859079537239097
5	18	-0.3279171187859333
5	19	-0.27019995076565806
5	20	-0.3155847902578799
5	21	-0.2731138974758238
5	22	-0.31257145712979195
5	23	-0.30456516448992943
5	24	-0.33088656288923346
5	25	-0.3256799632520426
5	26	-0.35472168024377027
5	27	-0.3373159176202378
5	28	-0.26748288322256286
5	29	-0.23861171127811787
5	30	-0.2862690076965748
5	31	-0.16475590310867005
5	32	-0.20973615959470493
5	33	-0.26277546034218346
5	34	-0.22018784443531558
5	35	-0.2638656828808905
5	36	-0.24135492513411805
5	37	-0.2522009464206576
5	38	-0.25630761867508056
5	39	-0.24780545774672902
5	40	-0.3285056960400041
5	41	-0.31806471008700754
5	42	-0.30876306870093373
5	43	-0.3550342282248522
5	44	-0.40982566983067414
5	45	-0.4076692557931878
5	46	-0.4119107928480972
5	47	-0.5090675686461239
5	48	-0.4495082662800982
5	49	-0.4309873487309588
5	50	-0.4367977432713488
6	1	-0.19465411160860668
6	2	-0.22449943899378155
6	3	-0.22862723608717062
6	4	-0.22219472433966558
6	5	-0.19291549873537406
6	6	-0.20107505815400373
6	7	-0.2739113083249691
6	8	-0.23441010573198184
6	9	-0.2843443879427596
6	10	-0.2681805829877746
6	11	-0.25282175454893263
6	12	-0.25561674459091677
6	13	-0.2407886151884
6	14	-0.29102022436000397
6	15	-0.32210452854614147
6	16	-0.3307712475179514
6	17	-0.31136342279334883
6	18	-0.3686702168630589
6	19	-0.2923994257457352
6	20	-0.31717817732527465
6	21	-0.3872065880911961
6	22	-0.3507636913995569
6	23	-0.36293274634901707
6	24	-0.3902925498200389
6	25	-0.3611336713674689
6	26	-0.40636230041830856
6	27	-0.40849013595713757
6	28	-0.3110683564972021
6	29	-0.3269985532675719
6	30	-0.32567673612332015
6	31	-0.30153542899048347
6	32	-0.3234504633188209
6	33	-0.34113734763882775
6	34	-0.3849304369912261
6	35	-0.36883739278059835
6	36	-0.3696334443195539
6	37	-0.3958900849307412
6	38	-0.3571698665167631
6	39	-0.38584119888339075
6	40	-0.3950680457417519
6	41	-0.4889329152157629
6	42	-0.4481365538873163
6	43	-0.48406433378404756
6	44	-0.4482311147730087
6	45	-0.5092258528745006
6	46	-0.5216301148809706
6	47	-0.6338944301879508
6	48	-0.6417215400358458
6	49	-0.6400318307184918
6	50	-0.6706833036915256
7	1	-0.16236019274276003
7	2	-0.2000240386109624
7	3	-0.198490549407083
7	4	-0.1863389789126515
7	5	-0.18195751051743514
7	6	-0.1734905569321441
7	7	-0.19222184402704556
7	8	-0.19290874549582782
7	9	-0.17438143602539213
7	10	-0.2439783228754564
7	11	-0.2027048120720778
7	12	-0.24293772994916907
7	13	-0.2236242300138258
7	14	-0.22948620871278536
7	15	-0.2520589984029371
7	16	-0.23529812200322908
7	17	-0.26268155072431654
7	18	-0.24716107748047733
7	19	-0.27809028571491723
7	20	-0.2708622430947756
7	21	-0.26063921299815007
7	22	-0.2956709627757306
7	23	-0.3108342386502798
7	24	-0.31117461343391284
7	25	-0.344589451532336
7	26	-0.3464477396081662
7	27	-0.3579738710077507
7	28	-0.2976057231164404
7	29	-0.3681950522004769
7	30	-0.30859717598768555
7	31	-0.2834917299903523
7	32	-0.35797919722628535
7	33	-0.3100527723659212
7	34	-0.303461021272156
7	35	-0.3232980173612887
7	36	-0.29574048379513584
7	37	-0.40095148671036185
7	38	-0.4100942269131025
7	39	-0.42792479689629204
7	40	-0.472777751267742
7	41	-0.39307942300541043
7	42	-0.37014260529835497
7	43	-0.43747981196980845
7	44	-0.4127257402764215
7	45	-0.4582586318028916
7	46	-0.5089526173909888
7	47	-0.4791858786258203
7	48	-0.5439498138382248
7	49	-0.48649327531970954
7	50	-0.5758498362072473
8	1	-0.12028684355175301
8	2	-0.14692635012030308
8	3	-0.13825628452654826
8	4	-0.1563741634126501
8	5	-0.147623289542004
8	6	-0.13751127687458364
8	7	-0.1565449006537989
8	8	-0.19815490256499402
8	9	-0.18825365954890572
8	10	-0.1840861795264388
8	11	-0.18889863169389542
8	12	-0.1764769408972029
8	13	-0.1998174638007768
8	14	-0.18450920226406708
8	15	-0.19831251517800297
8	16	-0.2293036395645629
8	17	-0.20866669792564352
8	18	-0.19785936122102044
8	19	-0.2062158672627774
8	20	-0.20747222983339952
8	21	-0.2451910929409351
8	22	-0.2523548092559835
8	23	-0.23515735128676596
8	24	-0.26357288216818375
8	25	-0.24804494216103734
8	26	-0.23833278735161656
8	27	-0.2428471222225994
8	28	-0.19646884444843948
8	29	-0.22958339257979077
8	30	-0.15021591677549395
8	31	-0.12615611736996613
8	32	-0.13800207735038583
8	33	-0.13446491057430876
8	34	-0.20773042820189858
8	35	-0.17280014380880004
8	36	-0.1913683954337202
8	37	-0.1985872131164541
8	38	-0.21448969236039517
8	39	-0.19794311095673578
8	40	-0.23940364469487463
8	41	-0.23073538447113862
8	42	-0.24282302045512694
8	43	-0.27481763044669094
8	44	-0.2724811304386634
8	45	-0.3295962124427376
8	46	-0.31062288932273674
8	47	-0.3425247217169982
8	48	-0.343202297968773
8	49	-0.409068161842022
8	50	-0.42313484565628556
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113	23	-0.046699969043821454
113	24	-0.06358480653413068
113	25	-0.12420998609673717
113	26	-0.10809635929773975
113	27	-0.0852677855811308
113	28	-0.04419109339105631
113	29	-0.04880243778367799
113	30	-0.0427975852843403
113	31	0.007984362009253232
113	32	-0.021912364404492735
113	33	-0.023454703953248668
113	34	0.032730641163837504
113	35	-0.04359205818484213
113	36	-0.005347407170688712
113	37	0.023543955454179866
113	38	-0.013250289713873542
113	39	0.06722868883569788
113	40	-0.022577514024305856
113	41	0.0625604995101483
113	42	0.0676969600122419
113	43	0.0697100353693827
113	44	0.008054105676045253
113	45	0.12945686262791156
113	46	0.13846041650338492
113	47	0.1662553854892863
113	48	0.16409538303485505
113	49	0.14357492466294275
113	50	0.15500246568835507
114	1	-0.04804083839914597
114	2	-0.05463661862992808
114	3	-0.05563158835465032
114	4	-0.056741425225133924
114	5	-0.04577286112611745
114	6	-0.0724427918470738
114	7	-0.05601752750433775
114	8	-0.03757428529480933
114	9	-0.07319608140397094
114	10	-0.0402245641490353
114	11	-0.03831583501155933
114	12	-0.0629774221858952
114	13	-0.0884713011867575
114	14	-0.06173249092829991
114	15	-0.06175394029540371
114	16	-0.1320001048359174
114	17	-0.03561859725492411
114	18	-0.04965050760310419
114	19	-0.023213506991687893
114	20	-0.05244382449934193
114	21	-0.0912012760341483
114	22	-0.0642137183691247
114	23	-0.06845037547263644
114	24	-0.051406828130822646
114	25	-0.06356835657792459
114	26	-0.09774204585037438
114	27	-0.06639125855249262
114	28	-0.08021883752405756
114	29	-0.04988314693024165
114	30	-0.07906988272513615
114	31	-0.03468169861726267
114	32	0.04147524619497034
114	33	0.007675147715431763
114	34	0.06109851858342097
114	35	0.058379509634661986
114	36	-0.015857269043351607
114	37	-0.01433164174951429
114	38	-0.016041867543272303
114	39	-0.007413420398584947
114	40	0.044405051663957806
114	41	0.07475546590691096
114	42	0.08576904501726546
114	43	-0.029292974650310555
114	44	-0.017002424983260767
114	45	0.02582623781741944
114	46	0.05315733569942438
114	47	-0.00900663549849412
114	48	0.03238893369025675
114	49	-0.08933241061757258
114	50	-0.0525830570896062
115	1	-0.05544937920471682
115	2	-0.06711098616913347
115	3	-0.0752973540430446
115	4	-0.09067447233019976
115	5	-0.08306162939221196
115	6	-0.11870673276169441
115	7	-0.0806545741806346
115	8	-0.09485219233976494
115	9	-0.10266193763683162
115	10	-0.109362192474876
115	11	-0.14030272262839105
115	12	-0.13139696970412018
115	13	-0.09431903930541097
115	14	-0.15409341095797657
115	15	-0.14749744941146758
115	16	-0.14779736826608314
115	17	-0.0971610462769803
115	18	-0.1227175014340105
115	19	-0.13856360293801373
115	20	-0.10722424151030197
115	21	-0.10089314473601974
115	22	-0.08287974423306821
115	23	-0.13692173917652894
115	24	-0.15957316185860293
115	25	-0.09998556051166219
115	26	-0.10926933379148096
115	27	-0.14737391657589427
115	28	-0.05377977715657778
115	29	-0.023167478601941127
115	30	-0.07984177112692237
115	31	-0.035280057527842246
115	32	-0.17782757408260608
115	33	-0.022160160954470598
115	34	-0.04297731699576701
115	35	-0.0400248076967884
115	36	-0.05441647680987671
115	37	-0.13079941901899161
115	38	-0.08390664293982297
115	39	-0.10805344326787036
115	40	-0.10337631879647802
115	41	-0.0667033144163014
115	42	-0.11725550095214743
115	43	-0.10666319126069368
115	44	-0.08377931105548697
115	45	-0.08407423026644523
115	46	-0.13126324777324783
115	47	-0.09826817216320194
115	48	-0.04660069368265596
115	49	-0.06317151441094637
115	50	-0.007029666557038183
116	1	-0.10457108917704971
116	2	-0.10131575092628253
116	3	-0.08533663160786631
116	4	-0.10970723576492958
116	5	-0.10810738351676008
116	6	-0.1345932663633178
116	7	-0.12401112147208693
116	8	-0.1170104458192327
116	9	-0.12398809401515365
116	10	-0.12438130180892415
116	11	-0.13666411631383113
116	12	-0.1307936661824698
116	13	-0.13752992270678988
116	14	-0.14703987583153122
116	15	-0.1940766659711457
116	16	-0.1103458362070242
116	17	-0.1881042968375226
116	18	-0.13805353205050608
116	19	-0.16976397125356613
116	20	-0.16051302229750775
116	21	-0.19133364236186168
116	22	-0.17241655903657005
116	23	-0.17577040622730777
116	24	-0.15142010311237186
116	25	-0.10286432447169602
116	26	-0.18866786838441385
116	27	-0.1267811563703276
116	28	-0.1260624129583121
116	29	-0.12102247025185164
116	30	-0.12536613135299035
116	31	-0.08913147558496348
116	32	-0.012028445374099306
116	33	-0.06330508070460894
116	34	-0.10052199156250907
116	35	-0.004825556312631818
116	36	0.048775763607537215
116	37	-0.042715989816066724
116	38	-0.050023508879434075
116	39	0.04119173993923653
116	40	0.0046810220876949415
116	41	0.0367413453610439
116	42	0.0539703422776725
116	43	0.021035096530063413
116	44	-0.02017428929288201
116	45	-0.06840393617886775
116	46	0.06860955606222774
116	47	0.11539352130073155
116	48	0.09349025389688848
116	49	0.011841571595319067
116	50	0.12420363355153086
117	1	-0.0498336710875833
117	2	-0.052593671973149014
117	3	-0.03782060784597263
117	4	-0.05753821012487492
117	5	-0.050641113953162176
117	6	-0.05058954343226674
117	7	-0.04072352439862925
117	8	-0.10484579505833125
117	9	-0.05715336936679449
117	10	-0.06402296723116763
117	11	-0.04841763354408357
117	12	-0.06789260884364268
117	13	-0.06477219327926775
117	14	-0.08222395621565681
117	15	-0.09190047730032092
117	16	-0.05882129203335751
117	17	-0.11956229732039247
117	18	-0.08980622144738959
117	19	-0.1198281803312824
117	20	-0.1311615938963655
117	21	-0.14460377393053392
117	22	-0.13402088915996302
117	23	-0.12052362110402726
117	24	-0.12278153317615192
117	25	-0.09372445190559375
117	26	-0.16384349467170267
117	27	-0.08210757292289372
117	28	-0.11847998230420842
117	29	-0.12025068722391552
117	30	-0.10642581542752794
117	31	-0.11311590404593375
117	32	-0.018431733402039185
117	33	-0.10747936688441229
117	34	-0.15476398302406835
117	35	-0.09809053709027893
117	36	-5.519507524525125E-4
117	37	-0.005212879132670878
117	38	-0.045934278939640194
117	39	0.05794178782988979
117	40	-0.006940429337561227
117	41	0.021669224486821292
117	42	-0.00353665937276304
117	43	0.011300466956022603
117	44	-0.0030374027668855774
117	45	-0.06744938454749416
117	46	0.05461598577927873
117	47	0.08225032479166572
117	48	0.07142892543892998
117	49	-0.015076170364913821
117	50	0.07843506279073864
118	1	-0.08481404234071022
118	2	-0.07392384175720679
118	3	-0.036346688678158046
118	4	-0.07111160690452323
118	5	-0.0705889677413225
118	6	-0.06935317025769905
118	7	-0.09418087786666973
118	8	-0.07825961240363455
118	9	-0.05695495388803806
118	10	-0.09287328771745962
118	11	-0.0856434948567184
118	12	-0.045648919702593105
118	13	-0.11840090992178176
118	14	-0.14990042623351485
118	15	-0.15559636200440963
118	16	-0.13125055961268117
118	17	-0.09868061418369223
118	18	-0.1369130199535391
118	19	-0.07528756373262269
118	20	-0.11614773343163165
118	21	-0.11841027879668076
118	22	-0.07768895843838663
118	23	-0.10348424415656865
118	24	-0.12001714226963145
118	25	-0.1377439033013843
118	26	-0.06763368630163313
118	27	-0.10731109929388083
118	28	0.0059886732732792325
118	29	-0.03829754356828374
118	30	-0.05834454682644008
118	31	0.01873872040521718
118	32	-0.09153084888765761
118	33	0.025321188472680944
118	34	-0.007949998373433687
118	35	0.04751407275156083
118	36	0.0647582120369421
118	37	0.09329648468585816
118	38	0.046084934628417784
118	39	0.04068660611973485
118	40	0.12038509353150317
118	41	0.14142368498291802
118	42	0.08224628056932204
118	43	0.12453635105742222
118	44	-0.04200017381760546
118	45	0.10742505490215848
118	46	0.15951040397624183
118	47	0.05525252653917079
118	48	0.18611928039454995
118	49	0.11089209668694622
118	50	0.15359247889434613
119	1	-0.08351825689496195
119	2	-0.07763092521821591
119	3	-0.06192569323422248
119	4	-0.07607464129145569
119	5	-0.0679436818478294
119	6	-0.08140875687069382
119	7	-0.11530359058269113
119	8	-0.09199687738065165
119	9	-0.09955781314324952
119	10	-0.12881938804390813
119	11	-0.15776877992449556
119	12	-0.14396350793603574
119	13	-0.1880636505261606
119	14	-0.14281085817422223
119	15	-0.16382468628086855
119	16	-0.1580836899162037
119	17	-0.18784886813201496
119	18	-0.21533661258991543
119	19	-0.18567843610276924
119	20	-0.20358389302399615
119	21	-0.23175090876760862
119	22	-0.13979646864072492
119	23	-0.1790442641189145
119	24	-0.2112214786089126
119	25	-0.1557022402447643
119	26	-0.16250303117554665
119	27	-0.1927553229992114
119	28	-0.14313866418942212
119	29	-0.037594142605577474
119	30	-0.03718609255328431
119	31	0.02492035344093324
119	32	-0.02554443748729085
119	33	-0.09675657077325184
119	34	-0.0671218778562448
119	35	0.018893152771454425
119	36	-0.03428199870692339
119	37	1.8361398915089922E-4
119	38	-0.0928896332071929
119	39	0.06142131783635563
119	40	0.010178016632011122
119	41	0.0031056566531191265
119	42	-0.009129134309709741
119	43	-0.0033378144960849454
119	44	0.03637325378886658
119	45	-0.06856128020575625
119	46	-0.010703274958764553
119	47	-0.0172390718449833
119	48	-0.011579017168415362
119	49	-0.03303285248594534
119	50	-0.044425530616031494
120	1	-0.07894697429542674
120	2	-0.07408276226425414
120	3	-0.04448162840557046
120	4	-0.045374609042902136
120	5	-0.05351594124925896
120	6	-0.08432366637705258
120	7	-0.0725234481786643
120	8	-0.05523303810784341
120	9	-0.07667251051797308
120	10	-0.06562846766476582
120	11	-0.127595675612433
120	12	-0.10090777735536705
120	13	-0.10550355578758541
120	14	-0.14063207255291132
120	15	-0.1322984376415164
120	16	-0.14722008468621794
120	17	-0.15421033842302023
120	18	-0.15643266133712785
120	19	-0.12773018787633106
120	20	-0.14067014683060108
120	21	-0.23121619455979925
120	22	-0.13596532661784266
120	23	-0.17641102174192724
120	24	-0.15334245765799892
120	25	-0.15015463200759882
120	26	-0.15715203864476024
120	27	-0.1269645473072316
120	28	-0.06295541750640155
120	29	-0.09696786705887916
120	30	-0.04290199747310908
120	31	0.03357018649752064
120	32	-0.033348325637575726
120	33	0.008004591711575415
120	34	-0.08158460051394911
120	35	-0.0025613809393547626
120	36	-0.038182183794631896
120	37	0.0012598222567135053
120	38	-0.020354293150305125
120	39	0.0882344506315178
120	40	0.026950435809702356
120	41	0.011802193858791554
120	42	0.02363354096721082
120	43	0.04580343301248746
120	44	0.08089738617988473
120	45	0.06636393330970947
120	46	0.061602453213687625
120	47	0.07308714785262538
120	48	0.0765277737397625
120	49	0.05434824715230491
120	50	0.008924580686759498
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6332.0
3	429.0
4	675.0
5	760.0
6	1252.0
7	2157.0
8	2605.0
9	3156.0
10	4911.0
11	6728.0
12	8160.0
13	11236.0
14	14586.0
15	17891.0
16	22577.0
17	26306.0
18	32504.0
19	45807.0
20	56588.0
21	38639.0
22	43434.0
23	56442.0
24	74684.0
25	68947.0
26	100145.0
27	128748.0
28	152050.0
29	159290.0
30	200698.0
31	251326.0
32	315281.0
33	380457.0
34	537575.0
35	806300.0
36	1268145.0
37	2291518.0
38	4482705.0
39	1.9161183E7
40	340837.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.846945822154154	19.215791554628304	12.572350196710328	24.364912426507214
2	17.583902580085468	21.287179899109983	35.26737461041674	25.861542910387815
3	17.937995813129113	25.0062366027121	32.76927172901314	24.286495855145642
4	11.84596093408512	16.004938214801225	37.49442052286239	34.65468032825126
5	12.71439084532294	38.14612211702549	37.07125686596924	12.068230171682327
6	32.53403649460734	37.209922519196695	17.270234061787747	12.985806924408214
7	27.242976462728734	33.68661581648902	22.317693399338832	16.752714321443417
8	29.443090821649182	33.108819234507244	20.194570174710304	17.25351976913327
9	26.613594342767794	12.633087796240114	19.44080120132131	41.312516659670784
10	17.320476544340234	26.892654270800588	35.19311273465877	20.593756450200402
11	36.2349735231724	21.469406097034664	22.94285356994414	19.3527668098488
12	21.88509460379608	26.014472739573456	31.589248410760582	20.511184245869877
13	29.194760515866946	19.951236163637358	25.626252608033706	25.227750712461987
14	20.64980170332844	20.246852944812886	29.458937590463457	29.644407761395215
15	25.247687695530235	28.35768997551141	23.508813271084104	22.88580905787425
16	24.77860791598154	26.70950070982728	25.578076117441395	22.933815256749785
17	21.393057572994742	27.34357067157655	26.904809243717136	24.358562511711572
18	21.81176313488929	28.2768913754764	27.760197389305887	22.151148100328424
19	22.262852782644135	28.008663564051112	28.075758664303052	21.652724989001698
20	24.769967166143093	25.79811809617545	27.836671057087997	21.59524368059346
21	24.994994066136933	25.83719584935468	27.35494165998566	21.812868424522726
22	22.598569680804697	25.543438429407615	27.710476852427675	24.147515037360012
23	22.30511902197199	27.735264250417735	27.686461169355752	22.27315555825452
24	23.7597130259175	26.15846931133167	27.807148152159805	22.27466951059102
25	22.24523548461143	28.088965379691338	27.773026040687494	21.892773095009737
26	22.014956114842	26.024899759930637	27.755724808876113	24.20441931635125
27	21.869888657721287	26.059570086164	27.58694253789343	24.483598718221277
28	23.813766736420966	26.02811840078193	28.166962367861125	21.991152494935974
29	21.793422071898902	28.143120033181425	27.995425609932894	22.06803228498678
30	21.947914617700416	26.37245797759415	29.834433221637703	21.845194183067736
31	22.308450928205783	27.883325795403525	27.71626624481317	22.09195703157752
32	21.564340593248303	26.182747734863288	29.889886665750915	22.363025006137494
33	21.769442601519394	26.31933135586187	27.848340542649925	24.06288549996881
34	24.367172547505504	25.628739616582585	27.972937076104547	22.031150759807367
35	22.04072365852158	26.111161810834815	30.13309477994033	21.71501975070328
36	22.1082560964253	28.564497601311288	27.298466036182706	22.02878026608071
37	22.136465511959315	25.971427926460773	29.92524500714302	21.966861554436896
38	24.30094923919918	26.401854162722387	27.494077535661376	21.803119062417046
39	21.941924433600402	26.513444989281137	27.56670154402895	23.977929033089517
40	21.993227709220367	25.908585139782414	28.036534096648136	24.06165305434908
41	23.893372690721254	26.174326589042547	27.727933840048518	22.204366880187678
42	21.6081188538028	26.384009366617356	29.881629631350805	22.126242148229043
43	21.99293874865308	25.983486127826342	27.768942793430618	24.25463233008995
44	21.90530347836148	26.249561269980394	29.726083800504043	22.11905145115408
45	21.682835985000718	26.18953898966866	29.880876368511878	22.246748656818742
46	21.9706468650209	26.265891364202247	27.488533391768737	24.274928379008117
47	21.75143213228096	26.29038828735307	30.03327444770668	21.92490513265928
48	24.2926778725864	26.473018849660924	26.944892606087055	22.28941067166562
49	21.806036551281963	26.53728366175424	27.589892254373023	24.066787532590773
50	21.958622729835167	26.378150276556696	29.41260361344666	22.25062338016148
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7554.0
1	6656.5
2	5759.0
3	14817.0
4	23875.0
5	24913.0
6	25951.0
7	20648.5
8	15346.0
9	21277.0
10	27208.0
11	41726.0
12	56244.0
13	94807.0
14	133370.0
15	142681.5
16	151993.0
17	196668.0
18	241343.0
19	247800.0
20	254257.0
21	294669.5
22	335082.0
23	388161.5
24	441241.0
25	501487.5
26	561734.0
27	638834.0
28	715934.0
29	798768.5
30	881603.0
31	974534.0
32	1067465.0
33	1165009.5
34	1262554.0
35	1351204.0
36	1439854.0
37	1510017.5
38	1580181.0
39	1627994.0
40	1675807.0
41	1709119.0
42	1742431.0
43	1775684.5
44	1808938.0
45	1831995.0
46	1855052.0
47	1810699.0
48	1766346.0
49	1737411.5
50	1708477.0
51	1652622.0
52	1596767.0
53	1575104.5
54	1553442.0
55	1515117.0
56	1476792.0
57	1671287.5
58	1865783.0
59	1578382.0
60	1290981.0
61	1135250.0
62	979519.0
63	862827.5
64	746136.0
65	657577.0
66	569018.0
67	497745.5
68	426473.0
69	362535.5
70	298598.0
71	244983.0
72	191368.0
73	165311.0
74	139254.0
75	109009.5
76	78765.0
77	63771.5
78	48778.0
79	38518.5
80	28259.0
81	22121.5
82	15984.0
83	13664.5
84	11345.0
85	10085.5
86	8826.0
87	7285.5
88	5745.0
89	4161.0
90	2577.0
91	2169.0
92	1761.0
93	1299.0
94	837.0
95	567.0
96	297.0
97	202.0
98	107.0
99	80.0
100	53.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10091230092255697
2	2.0563528063946403E-4
3	0.014005690442303495
4	2.4419189575936354E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	2.4419189575936354E-4
20	2.249135881994138E-5
21	0.0
22	2.6025715205932164E-4
23	9.317848653975714E-5
24	0.0026057845718532083
25	0.07119157676763445
26	0.08423977793446044
27	0.11551240584795892
28	0.18429098111933967
29	0.2002084370613382
30	0.2275482902326069
31	0.25012640143656806
32	0.28404337053703965
33	0.2745488040637644
34	0.29552038963772975
35	0.3265648909117689
36	0.3570342560102694
37	0.3655295635416873
38	0.37989190267384987
39	0.3983187516499018
40	0.4025342749030108
41	0.40621643164696125
42	0.4383373050930975
43	0.46857533050087874
44	0.5831173948683201
45	0.5697221841654151
46	0.4997483538253174
47	0.4965963505392657
48	0.5185639820038285
49	0.5348059561230861
50	0.5540842636830359
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3.1123064E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.082112441505664
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.0766049830692	41.60651872020622
2	8.885025541750897	8.899616964552367
3	2.9154827231201517	4.380406006817117
4	1.4439101242024512	2.892562767829423
5	0.820451483857915	2.0544971733686133
6	0.5491452017066996	1.6501411043152943
7	0.3837126034692262	1.3451996426518067
8	0.28524404350771	1.1428499408178294
9	0.20751982777039452	0.9353728213414909
>10	1.23645646602108	11.38929956405466
>50	0.10462775922248646	3.6257877930554674
>100	0.07532608701895434	7.565145553816424
>500	0.010031339382365416	3.4736746115737716
>1k	0.006230665072719473	5.622805104711981
>5k	1.541005519006915E-4	0.47942878542441375
>10k+	7.705027584482138E-5	2.936693445462988
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	499358	1.6044628510868981	Illumina PCR Primer Index 1 (95% over 24bp)
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT	90716	0.2914751581014003	Illumina Paired End PCR Primer 2 (96% over 29bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	64127	0.20604333814948297	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	56933	0.1829286473851032	No Hit
CTTATACACATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCC	46148	0.14827588954609353	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35456	0.11392194547426308	No Hit
CATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTG	35035	0.1125692508938066	Illumina Paired End PCR Primer 2 (96% over 26bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.2130512599916254E-6	0.0
2	0.0	3.2130512599916254E-6	0.0	6.426102519983251E-6	0.0
3	0.0	3.2130512599916254E-6	0.0	9.639153779974876E-6	0.0
4	0.0	3.2130512599916254E-6	0.0	9.639153779974876E-6	0.0
5	3.2130512599916254E-6	1.2852205039966502E-5	0.0	1.6065256299958127E-5	0.0
6	6.426102519983251E-6	1.2852205039966502E-5	0.0	1.6065256299958127E-5	3.2130512599916254E-6
7	1.6065256299958127E-5	1.2852205039966502E-5	0.0	2.5704410079933003E-5	3.2130512599916254E-6
8	2.891746133992463E-5	1.2852205039966502E-5	0.0	4.1769666379891134E-5	3.2130512599916254E-6
9	2.891746133992463E-5	1.927830755994975E-5	0.0	8.353933275978227E-5	3.2130512599916254E-6
10	3.2130512599916255E-5	1.927830755994975E-5	0.0	2.0563528063946403E-4	6.426102519983251E-6
11	3.2130512599916255E-5	3.85566151198995E-5	0.0	2.4419189575936354E-4	6.426102519983251E-6
12	3.534356385990788E-5	5.462187141985763E-5	0.0	2.9560071591922953E-4	9.639153779974876E-6
13	4.498271763988276E-5	5.783492267984926E-5	0.0	3.437964848191039E-4	9.639153779974876E-6
14	9.960458905974039E-5	5.783492267984926E-5	0.0	4.305488688388778E-4	9.639153779974876E-6
15	1.124567940997069E-4	6.426102519983251E-5	0.0	5.526448167185596E-4	9.639153779974876E-6
16	1.4137425543963152E-4	6.747407645982413E-5	0.0	6.651016108182665E-4	1.2852205039966502E-5
17	1.4137425543963152E-4	6.747407645982413E-5	0.0	7.614931486180153E-4	1.2852205039966502E-5
18	1.4458730669962314E-4	6.747407645982413E-5	0.0	9.0929350657763E-4	1.2852205039966502E-5
19	1.4458730669962314E-4	6.747407645982413E-5	0.0	0.0010217503006773368	1.2852205039966502E-5
20	1.510134092196064E-4	6.747407645982413E-5	0.0	0.001175976761156935	1.2852205039966502E-5
21	1.54226460479598E-4	6.747407645982413E-5	0.0	0.001294859657776625	2.249135881994138E-5
22	1.54226460479598E-4	6.747407645982413E-5	0.0	0.001407316451876332	2.249135881994138E-5
23	1.5743951173958964E-4	7.7113230239799E-5	0.0	0.0015197732459760389	2.249135881994138E-5
24	1.5743951173958964E-4	7.7113230239799E-5	0.0	0.0016547213988956872	2.249135881994138E-5
25	1.606525629995813E-4	7.7113230239799E-5	0.0	0.0018121609106352768	2.249135881994138E-5
26	1.638656142595729E-4	7.7113230239799E-5	0.0	0.0019728134736348582	2.249135881994138E-5
27	1.7350476803954778E-4	7.7113230239799E-5	0.0	0.002486901675233518	2.249135881994138E-5
28	1.9278307559949753E-4	8.675238401977389E-5	0.0	0.0033994082330711397	2.249135881994138E-5
29	1.9278307559949753E-4	9.960458905974039E-5	0.0	0.004681415685807798	2.249135881994138E-5
30	1.9278307559949753E-4	9.960458905974039E-5	0.0	0.006114436547764063	2.249135881994138E-5
31	1.9920917811948078E-4	9.960458905974039E-5	0.0	0.007698470818939935	2.249135881994138E-5
32	1.9920917811948078E-4	1.0603069157972364E-4	0.0	0.009616662421154935	2.249135881994138E-5
33	1.9920917811948078E-4	1.0603069157972364E-4	0.0	0.013340588831485228	3.2130512599916255E-5
34	2.024222293794724E-4	1.0603069157972364E-4	0.0	0.019185129073409996	3.2130512599916255E-5
35	2.152744344194389E-4	1.0603069157972364E-4	0.0	0.027108513480549344	3.2130512599916255E-5
36	2.152744344194389E-4	1.0603069157972364E-4	0.0	0.03972937882979645	3.2130512599916255E-5
37	2.1848748567943052E-4	1.1888289661969015E-4	0.0	0.06164881452545932	3.2130512599916255E-5
38	2.1848748567943052E-4	1.3494815291964827E-4	0.0	0.09285075531123799	3.2130512599916255E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGTCT	78340	0.0	35.943027	38
CCGTCTT	78995	0.0	35.583622	39
TATGCCG	80875	0.0	35.23822	35
CGTCTTC	79690	0.0	35.148594	40
ATGCCGT	81460	0.0	34.643635	36
TGCCGTC	81670	0.0	34.490932	37
ACCGATA	70080	0.0	33.14427	25
GTCTTCT	84970	0.0	33.091812	41
CCGATAT	70215	0.0	33.046726	26
TATCGTA	70140	0.0	32.987835	30
ATCGTAT	71005	0.0	32.95017	31
ATATCGT	70230	0.0	32.938713	29
GACCGAT	85420	0.0	32.935528	24
AGACCGA	87715	0.0	32.836777	23
CGATATC	70830	0.0	32.795666	27
GATATCG	70890	0.0	32.74545	28
CGTATGC	87195	0.0	32.740288	33
TCGTATG	86870	0.0	32.728687	32
GCGGGCT	90840	0.0	32.6305	8
TGAGCGG	91465	0.0	32.508324	5
>>END_MODULE