FastQCFastQC Report
Thu 26 May 2016
SRR522076_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522076_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32573398
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT2359330.7243119062985077Illumina PCR Primer Index 1 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT958170.29415721380987025No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT855450.2626222784617067Illumina Paired End PCR Primer 2 (96% over 29bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT819660.25163478492480273No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT489380.15023916141631893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT436350.034.24270639
GCCGTCT438900.034.09348338
TATGCCG444200.033.76096335
GGTATCA424550.033.445171
CGTCTTC446950.033.3863840
ATGCCGT464650.032.17014336
TGCCGTC468200.031.89801837
GACCGAT478450.031.2664724
ACCGATA346400.031.12557225
ATATCGT344400.031.05882529
TATCGTA345200.031.01244230
ATCGTAT349050.030.92894431
TCGTATG484300.030.89737932
CCGATAT349250.030.84041226
CGATATC349550.030.82695627
GATATCG350850.030.7548928
CGTATGC488150.030.74014933
AGACCGA497250.030.63788823
GCGGGCT520650.030.1988148
TGAGCGG529150.029.8099085
Produced by FastQC (version 0.11.5)