FastQCFastQC Report
Thu 26 May 2016
SRR522075_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522075_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34518051
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT5412031.5678840036478305Illumina PCR Primer Index 1 (95% over 24bp)
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT1127110.3265277057502465Illumina Paired End PCR Primer 2 (96% over 29bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT681420.1974097552610951No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT604690.1751808061237293No Hit
CTTATACACATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCC529150.1532966041448864No Hit
CATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTG398290.11538600484714504Illumina Paired End PCR Primer 2 (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT368790.10683975175771077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT872300.035.8165838
CCGTCTT878150.035.52277839
CGTCTTC880900.035.24390440
TATGCCG899200.035.15885535
ATGCCGT909200.034.4471936
TGCCGTC906700.034.4260537
GTCTTCT946900.032.94349341
ACCGATA763950.032.7830425
TATCGTA764300.032.66094230
ATCGTAT775150.032.62263531
ATATCGT765000.032.61323529
CCGATAT766900.032.5982226
CTTCTGC962300.032.5752243
GACCGAT959200.032.53806724
TCGTATG971850.032.47164532
CGATATC772050.032.4199127
AGACCGA987350.032.3932523
GATATCG773550.032.38598328
CGTATGC977450.032.38530333
TTCTGCT967900.032.3644844