SRR522075_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR522075_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34518051
Sequences flagged as poor quality	0
Sequence length	50
%GC	45

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	541203	1.5678840036478305	Illumina PCR Primer Index 1 (95% over 24bp)
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT	112711	0.3265277057502465	Illumina Paired End PCR Primer 2 (96% over 29bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	68142	0.1974097552610951	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	60469	0.1751808061237293	No Hit
CTTATACACATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCC	52915	0.1532966041448864	No Hit
CATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTG	39829	0.11538600484714504	Illumina Paired End PCR Primer 2 (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36879	0.10683975175771077	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GCCGTCT	87230	35.81658	38
CCGTCTT	87815	35.522778	39
CGTCTTC	88090	35.243904	40
TATGCCG	89920	35.158855	35
ATGCCGT	90920	34.44719	36
TGCCGTC	90670	34.42605	37
GTCTTCT	94690	32.943493	41
ACCGATA	76395	32.78304	25
TATCGTA	76430	32.660942	30
ATCGTAT	77515	32.622635	31
ATATCGT	76500	32.613235	29
CCGATAT	76690	32.59822	26
CTTCTGC	96230	32.57522	43
GACCGAT	95920	32.538067	24
TCGTATG	97185	32.471645	32
CGATATC	77205	32.41991	27
AGACCGA	98735	32.39325	23
GATATCG	77355	32.385983	28
CGTATGC	97745	32.385303	33
TTCTGCT	96790	32.36448	44