##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR522075_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 34518051 Sequences flagged as poor quality 0 Sequence length 50 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.022941214149085 40.0 39.0 40.0 38.0 40.0 2 38.894468925838254 40.0 39.0 40.0 38.0 40.0 3 38.863309373985224 40.0 39.0 40.0 37.0 40.0 4 38.912790006596836 40.0 39.0 40.0 38.0 40.0 5 38.89846538554567 40.0 39.0 40.0 38.0 40.0 6 38.93300354646327 40.0 39.0 40.0 38.0 40.0 7 38.88437475800705 40.0 39.0 40.0 37.0 40.0 8 38.868037798541984 40.0 39.0 40.0 37.0 40.0 9 38.896078460513316 40.0 39.0 40.0 37.0 40.0 10 38.82723291648187 40.0 39.0 40.0 37.0 40.0 11 38.87020753286447 40.0 39.0 40.0 37.0 40.0 12 38.79237390894405 40.0 39.0 40.0 36.0 40.0 13 38.78588889621839 40.0 39.0 40.0 36.0 40.0 14 38.75548271830296 40.0 39.0 40.0 36.0 40.0 15 38.715564270995486 40.0 39.0 40.0 36.0 40.0 16 38.680763030334475 40.0 39.0 40.0 36.0 40.0 17 38.616014907678306 40.0 39.0 40.0 36.0 40.0 18 38.59390549020279 40.0 39.0 40.0 36.0 40.0 19 38.56428776931815 40.0 38.0 40.0 36.0 40.0 20 38.52538867272663 40.0 38.0 40.0 35.0 40.0 21 38.447018894548826 40.0 38.0 40.0 35.0 40.0 22 38.35855332620025 40.0 38.0 40.0 35.0 40.0 23 38.33222316057184 40.0 38.0 40.0 35.0 40.0 24 38.25353775043672 40.0 38.0 40.0 35.0 40.0 25 38.177299378809074 40.0 38.0 40.0 35.0 40.0 26 37.999653282857714 40.0 38.0 40.0 35.0 40.0 27 37.906240911458184 39.0 38.0 40.0 35.0 40.0 28 38.023116571674336 40.0 38.0 40.0 35.0 40.0 29 37.89732783580394 40.0 38.0 40.0 35.0 40.0 30 37.78830230015014 40.0 38.0 40.0 34.0 40.0 31 37.497246556591506 40.0 38.0 40.0 33.0 40.0 32 37.78076853180384 40.0 38.0 40.0 34.0 40.0 33 37.78065557641131 40.0 38.0 40.0 34.0 40.0 34 37.77628542816627 40.0 38.0 40.0 34.0 40.0 35 37.62681178030591 40.0 38.0 40.0 33.0 40.0 36 37.784667535255686 40.0 38.0 40.0 35.0 40.0 37 37.71835289889339 40.0 38.0 40.0 35.0 40.0 38 37.6391923750272 40.0 38.0 40.0 34.0 40.0 39 37.53385519941436 40.0 38.0 40.0 33.0 40.0 40 37.484544535843 40.0 38.0 40.0 33.0 40.0 41 37.38533285091908 40.0 38.0 40.0 34.0 40.0 42 37.257282023252124 40.0 38.0 40.0 33.0 40.0 43 37.169445227368136 40.0 38.0 40.0 33.0 40.0 44 37.044436315364386 39.0 38.0 40.0 33.0 40.0 45 36.95916571303519 39.0 38.0 40.0 33.0 40.0 46 36.743253348805816 39.0 38.0 40.0 33.0 40.0 47 36.659471561705494 39.0 38.0 40.0 32.0 40.0 48 36.52024710201628 39.0 37.0 40.0 32.0 40.0 49 36.39655570356507 39.0 37.0 40.0 31.0 40.0 50 36.37930212224323 39.0 37.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1 1 -0.2249841447652372 1 2 -0.2339187190745804 1 3 -0.24891653618644938 1 4 -0.259528653855682 1 5 -0.23769181916613746 1 6 -0.2353682001122266 1 7 -0.25458013709850746 1 8 -0.26346664790991525 1 9 -0.25503152528717266 1 10 -0.2802329800505561 1 11 -0.28133067927279853 1 12 -0.3067084305855019 1 13 -0.31528070964563426 1 14 -0.3378607379077039 1 15 -0.3314646759323523 1 16 -0.31382922012294756 1 17 -0.3629893892112932 1 18 -0.3488306456363759 1 19 -0.38180758934185377 1 20 -0.3873914898130906 1 21 -0.4301967083553464 1 22 -0.3763300638562228 1 23 -0.4499333477483063 1 24 -0.42421359771535805 1 25 -0.44796602597699575 1 26 -0.5054821742513909 1 27 -0.49334350479090716 1 28 -0.4141493316293534 1 29 -0.4117962704314735 1 30 -0.3991581394015711 1 31 -0.2934647338462497 1 32 -0.33619783459876373 1 33 -0.3217617369800294 1 34 -0.3586175107522678 1 35 -0.34016598595888325 1 36 -0.3470690471121429 1 37 -0.3437378737159662 1 38 -0.4006330571644128 1 39 -0.36973947579866007 1 40 -0.3874559730050606 1 41 -0.3698282548575662 1 42 -0.426232448115897 1 43 -0.4589777136540576 1 44 -0.34264625795329096 1 45 -0.4202482652080093 1 46 -0.48418965500093236 1 47 -0.5285965310295282 1 48 -0.4918327172880268 1 49 -0.5474240093606042 1 50 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-0.10086496271195244 120 43 -0.1571797805508126 120 44 -0.2559242038966971 120 45 -0.14053135623780122 120 46 -0.15542909129307247 120 47 -0.18613488158232627 120 48 -0.09670308561253194 120 49 -0.14469928501601004 120 50 -0.16881938402621444 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7731.0 3 542.0 4 879.0 5 1074.0 6 1810.0 7 3030.0 8 3565.0 9 4445.0 10 6591.0 11 9036.0 12 10763.0 13 15199.0 14 19622.0 15 24149.0 16 30651.0 17 34918.0 18 44198.0 19 63093.0 20 59882.0 21 47918.0 22 56250.0 23 73035.0 24 90363.0 25 87745.0 26 123724.0 27 156638.0 28 187430.0 29 200558.0 30 251624.0 31 313072.0 32 390128.0 33 476234.0 34 672613.0 35 1000654.0 36 1553785.0 37 2764639.0 38 5122204.0 39 2.0357228E7 40 251031.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.50860855669203 19.562830223304523 12.61570274760925 24.312858472394193 2 17.547002332129296 21.411090174478176 35.45794882537879 25.583958668013736 3 17.889036444573776 25.27338290427889 32.82105174899263 24.016528902154704 4 11.786072167874503 16.197393748750454 37.69948658286755 34.3170475005075 5 12.68543812047789 38.29691021662839 37.15978633903751 11.857865323856204 6 32.35430934382709 37.50207391489166 17.325937666642883 12.817679074638368 7 27.081079983339734 33.790633196526656 22.470182340248584 16.658104479885033 8 29.31060331303178 33.39726220347725 20.159602869814407 17.132531613676566 9 26.554653969310145 12.627285938015445 19.482620846698442 41.33543924597597 10 17.154911208631102 27.05109856868802 35.38738035933721 20.406609863343675 11 36.15010302870228 21.5725534445731 23.030222650751632 19.247120875972982 12 21.80373103915977 26.084323242931646 31.73501018351239 20.376935534396193 13 29.078463902843183 20.04919976507364 25.734859711517316 25.137476620565856 14 20.555334946344452 20.261772601239855 29.562827866498026 29.62006458591767 15 25.127224013893485 28.4917274153167 23.558369503538888 22.822679067250927 16 24.673525165137512 26.843436206754546 25.659887344160886 22.82315128394706 17 21.330949421217323 27.503951483239884 26.93538809592697 24.229710999615826 18 21.72994355909608 28.458973538222075 27.804437741864398 22.00664516081745 19 22.191075548518207 28.150373414854368 28.09936530874592 21.559185727881502 20 24.71329031695852 25.96319116584272 27.845164806391836 21.478353710806918 21 24.906041189869036 25.96479737514728 27.38415908824053 21.74500234674316 22 22.513878122363877 25.6960731036603 27.721546886931275 24.06850188704455 23 22.233291708806153 27.887162350923706 27.689745454954206 22.18980048531593 24 23.65928192179796 26.310262998336725 27.83133671133402 22.19911836853129 25 22.16213124455035 28.232221718246684 27.802610448907622 21.803036588295342 26 21.967884888688445 26.14598743210786 27.77062305014018 24.115504629063516 27 21.78085325997106 26.212019806398256 27.601356448591847 24.40577048503884 28 23.75822028099473 26.169407899462538 28.18140339831352 21.89096842122921 29 21.76114548116693 28.268938980862252 27.99997410840838 21.969941429562432 30 21.90888767351828 26.474608742477717 29.845300683689537 21.77120290031447 31 22.238722534856922 27.94440189406358 27.773287504961345 22.043588066118154 32 21.468795068631085 26.297782610637512 29.957016561559136 22.27640575917227 33 21.675179354672512 26.47740953884894 27.854005729607778 23.993405376870772 34 24.29093765248985 25.741724266919913 28.006156165989843 21.9611819146004 35 21.928696053994976 26.24627738459787 30.173505654346837 21.651520907060316 36 22.0602156601093 28.647500387326236 27.341502309464495 21.950781643099972 37 22.03864443624309 26.05455630727544 29.996344862499402 21.91045439398207 38 24.271474116931227 26.5146293911429 27.51229638399891 21.70160010792696 39 21.851081174650915 26.624483411044764 27.62628845968281 23.89814695462151 40 21.912541515761653 26.01113282777043 28.067391484055705 24.008934172412207 41 23.85760351681477 26.258935803718092 27.751230261722608 22.132230417744523 42 21.52929038875491 26.45260026047593 29.95504875513855 22.06306059563062 43 21.928193547720088 26.099062051278842 27.80708308330056 24.165661317700515 44 21.857542707259732 26.32352388831692 29.785176000965762 22.033757403457585 45 21.618358948943165 26.264964916956462 29.953658206763794 22.163017927336572 46 21.884811900878336 26.3497519117104 27.548513432424254 24.216922754987007 47 21.70024592464957 26.365635238942353 30.10287094988902 21.83124788651906 48 24.244002590382728 26.532336115001947 26.998647524797974 22.225013769817348 49 21.74291116225893 26.590928824014398 27.668901036714733 23.99725897701194 50 21.922393134074152 26.433594169202323 29.46746882843387 22.176543868289652 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 7613.0 1 6744.5 2 5876.0 3 15433.0 4 24990.0 5 26253.0 6 27516.0 7 21958.5 8 16401.0 9 22739.0 10 29077.0 11 44737.5 12 60398.0 13 102100.5 14 143803.0 15 154069.5 16 164336.0 17 214522.0 18 264708.0 19 273272.0 20 281836.0 21 328024.5 22 374213.0 23 434435.5 24 494658.0 25 564909.5 26 635161.0 27 722831.0 28 810501.0 29 904433.0 30 998365.0 31 1104138.5 32 1209912.0 33 1318702.5 34 1427493.0 35 1527557.0 36 1627621.0 37 1704356.0 38 1781091.0 39 1834725.5 40 1888360.0 41 1923050.5 42 1957741.0 43 1992265.0 44 2026789.0 45 2049422.5 46 2072056.0 47 2018253.5 48 1964451.0 49 1929130.5 50 1893810.0 51 1832433.0 52 1771056.0 53 1744178.0 54 1717300.0 55 1667092.0 56 1616884.0 57 1822984.5 58 2029085.0 59 1721997.0 60 1414909.0 61 1235924.0 62 1056939.0 63 926952.0 64 796965.0 65 701969.0 66 606973.0 67 528543.5 68 450114.0 69 382299.0 70 314484.0 71 257019.5 72 199555.0 73 172177.5 74 144800.0 75 113280.5 76 81761.0 77 65898.5 78 50036.0 79 39475.5 80 28915.0 81 22868.5 82 16822.0 83 14324.5 84 11827.0 85 10354.5 86 8882.0 87 7378.0 88 5874.0 89 4292.0 90 2710.0 91 2324.5 92 1939.0 93 1407.5 94 876.0 95 630.0 96 384.0 97 257.0 98 130.0 99 92.5 100 55.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08753101384548044 2 0.0019352193436413894 3 0.006570475256554896 4 0.0014601056125677547 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 2.3176279564567537E-5 20 9.560215320384108E-5 21 0.0 22 1.5354285211525992E-4 23 0.002149599929613639 24 0.0 25 0.11897832817965301 26 0.09246756139273332 27 0.13489753520556533 28 0.17950028522757555 29 0.19326699528892868 30 0.2569119560081767 31 0.24439386800836466 32 0.30292556204868 33 0.3169732844997535 34 0.2949905833327612 35 0.32971734122532004 36 0.35338611673063464 37 0.3631838889165556 38 0.40339763099602577 39 0.4136965902275305 40 0.4365310196685207 41 0.41912563371553047 42 0.4440082668630393 43 0.5240330631645455 44 0.5768575983620859 45 0.6162920380411976 46 0.4872320282509578 47 0.5270401854380481 48 0.5222426955681826 49 0.535615408876938 50 0.6028642810684763 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 3.4518051E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.08010754838364 #Duplication Level Percentage of deduplicated Percentage of total 1 82.7077840406981 40.5930693580595 2 8.900149639575409 8.736406030141378 3 2.965851680176974 4.366929583069205 4 1.5424203767499742 3.028086319028285 5 0.9044091475393802 2.2194249114487365 6 0.5830833877881174 1.7170677229390012 7 0.39432552699649087 1.3547477491842577 8 0.28106161840765204 1.103562756733626 9 0.23188332874599424 1.0242772842177537 >10 1.2898272888123925 11.654106990865689 >50 0.10388753612243379 3.534956749238733 >100 0.07823784328652009 7.748826524224997 >500 0.01027497293824366 3.5321634521831866 >1k 0.006549627261374444 5.900451873222856 >5k 1.712921430293397E-4 0.5066312287227908 >10k+ 8.269275778356604E-5 2.9792914667197934 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT 541203 1.5678840036478305 Illumina PCR Primer Index 1 (95% over 24bp) TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT 112711 0.3265277057502465 Illumina Paired End PCR Primer 2 (96% over 29bp) GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 68142 0.1974097552610951 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 60469 0.1751808061237293 No Hit CTTATACACATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCC 52915 0.1532966041448864 No Hit CATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTG 39829 0.11538600484714504 Illumina Paired End PCR Primer 2 (96% over 26bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36879 0.10683975175771077 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 2.897034945570942E-6 0.0 0.0 2 0.0 0.0 2.897034945570942E-6 0.0 0.0 3 0.0 2.897034945570942E-6 2.897034945570942E-6 5.794069891141884E-6 0.0 4 0.0 5.794069891141884E-6 2.897034945570942E-6 1.448517472785471E-5 0.0 5 2.897034945570942E-6 1.7382209673425653E-5 2.897034945570942E-6 2.3176279564567537E-5 0.0 6 8.691104836712826E-6 1.7382209673425653E-5 2.897034945570942E-6 2.3176279564567537E-5 0.0 7 8.691104836712826E-6 1.7382209673425653E-5 2.897034945570942E-6 2.897034945570942E-5 0.0 8 1.1588139782283768E-5 1.7382209673425653E-5 2.897034945570942E-6 4.9249594074706016E-5 0.0 9 1.1588139782283768E-5 1.7382209673425653E-5 2.897034945570942E-6 1.1588139782283768E-4 0.0 10 1.448517472785471E-5 1.7382209673425653E-5 2.897034945570942E-6 1.99895411244395E-4 2.897034945570942E-6 11 2.0279244618996595E-5 2.3176279564567537E-5 2.897034945570942E-6 2.49145005319101E-4 2.897034945570942E-6 12 2.0279244618996595E-5 3.766145429242225E-5 2.897034945570942E-6 2.897034945570942E-4 2.897034945570942E-6 13 3.766145429242225E-5 4.055848923799319E-5 2.897034945570942E-6 3.302619837950874E-4 2.897034945570942E-6 14 1.0429325804055392E-4 4.6352559129135074E-5 2.897034945570942E-6 4.084819273255028E-4 8.691104836712826E-6 15 1.100873279316958E-4 5.50436639658479E-5 2.897034945570942E-6 5.011870455837729E-4 8.691104836712826E-6 16 1.2746953760512145E-4 5.50436639658479E-5 2.897034945570942E-6 5.562307095496209E-4 8.691104836712826E-6 17 1.2746953760512145E-4 5.50436639658479E-5 2.897034945570942E-6 6.576269326446038E-4 8.691104836712826E-6 18 1.2746953760512145E-4 5.794069891141884E-5 2.897034945570942E-6 7.735083304674415E-4 1.1588139782283768E-5 19 1.3036657255069238E-4 6.373476880256073E-5 2.897034945570942E-6 8.604193788345698E-4 1.1588139782283768E-5 20 1.3616064244183427E-4 6.373476880256073E-5 2.897034945570942E-6 9.99477056221975E-4 1.1588139782283768E-5 21 1.3616064244183427E-4 6.373476880256073E-5 2.897034945570942E-6 0.001100873279316958 1.1588139782283768E-5 22 1.3616064244183427E-4 6.952883869370261E-5 2.897034945570942E-6 0.0012167546771397956 1.448517472785471E-5 23 1.3616064244183427E-4 7.53229085848445E-5 2.897034945570942E-6 0.0013123568303436367 1.7382209673425653E-5 24 1.3616064244183427E-4 7.821994353041544E-5 2.897034945570942E-6 0.0014166500883841906 1.7382209673425653E-5 25 1.3616064244183427E-4 7.821994353041544E-5 2.897034945570942E-6 0.001549913695880454 1.7382209673425653E-5 26 1.448517472785471E-4 7.821994353041544E-5 2.897034945570942E-6 0.001642618814138724 1.7382209673425653E-5 27 1.5354285211525992E-4 8.401401342155732E-5 2.897034945570942E-6 0.0020134392871718047 1.7382209673425653E-5 28 1.651309918975437E-4 8.98080833126992E-5 2.897034945570942E-6 0.0026536840101429827 1.7382209673425653E-5 29 1.7382209673425653E-4 1.0429325804055392E-4 2.897034945570942E-6 0.0035343826335965493 2.0279244618996595E-5 30 1.7382209673425653E-4 1.0429325804055392E-4 2.897034945570942E-6 0.004698990681716068 2.0279244618996595E-5 31 1.7382209673425653E-4 1.0429325804055392E-4 2.897034945570942E-6 0.005993965302386279 2.0279244618996595E-5 32 1.7671913167982746E-4 1.0719029298612486E-4 2.897034945570942E-6 0.007859655807333965 2.0279244618996595E-5 33 1.7671913167982746E-4 1.100873279316958E-4 2.897034945570942E-6 0.011092746806591136 2.0279244618996595E-5 34 1.7671913167982746E-4 1.1298436287726674E-4 2.897034945570942E-6 0.015985838829660457 2.0279244618996595E-5 35 1.7671913167982746E-4 1.1298436287726674E-4 2.897034945570942E-6 0.022721445078112898 2.0279244618996595E-5 36 1.7671913167982746E-4 1.1298436287726674E-4 2.897034945570942E-6 0.03331300483912026 2.0279244618996595E-5 37 1.8830727146211122E-4 1.1588139782283768E-4 2.897034945570942E-6 0.05277818263841142 2.0279244618996595E-5 38 1.8830727146211122E-4 1.2746953760512145E-4 2.897034945570942E-6 0.07956706478010592 2.0279244618996595E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCGTCT 87230 0.0 35.81658 38 CCGTCTT 87815 0.0 35.522778 39 CGTCTTC 88090 0.0 35.243904 40 TATGCCG 89920 0.0 35.158855 35 ATGCCGT 90920 0.0 34.44719 36 TGCCGTC 90670 0.0 34.42605 37 GTCTTCT 94690 0.0 32.943493 41 ACCGATA 76395 0.0 32.78304 25 TATCGTA 76430 0.0 32.660942 30 ATCGTAT 77515 0.0 32.622635 31 ATATCGT 76500 0.0 32.613235 29 CCGATAT 76690 0.0 32.59822 26 CTTCTGC 96230 0.0 32.57522 43 GACCGAT 95920 0.0 32.538067 24 TCGTATG 97185 0.0 32.471645 32 CGATATC 77205 0.0 32.41991 27 AGACCGA 98735 0.0 32.39325 23 GATATCG 77355 0.0 32.385983 28 CGTATGC 97745 0.0 32.385303 33 TTCTGCT 96790 0.0 32.36448 44 >>END_MODULE