FastQCFastQC Report
Thu 26 May 2016
SRR522074_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522074_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27195847
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT11603504.2666440945928255Illumina PCR Primer Index 1 (95% over 24bp)
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT3036321.1164645837285376Illumina Paired End PCR Primer 2 (96% over 29bp)
CTTATACACATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCC1034940.3805507510025336No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT795990.2926880710867361No Hit
CATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTG706610.25982275896757323Illumina Paired End PCR Primer 2 (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT690720.25397995510123295No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430310.15822636448866623No Hit
ATACACATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTC399000.14671357726052806No Hit
ACACATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTT375010.13789237746483868No Hit
CTTATACACATCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCC306700.11277457179399487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT1837300.038.72447244
CTTCTGC1865850.038.119743
CGTCTTC1849900.037.94683540
CCGTCTT1892400.037.163339
GCCGTCT1897900.037.08321838
GTCTTCT1901950.036.9551741
TCTTCTG1930450.036.3254642
TATGCCG1957800.036.09274735
ATGCCGT1957300.035.98776636
TGCCGTC1955150.035.97444537
ACCGATA1587850.034.06107725
CCGATAT1584350.033.98973526
GACCGAT2083650.033.933724
ATATCGT1580400.033.8526629
ATCGTAT1594750.033.84866331
GATATCG1594750.033.8468228
CGATATC1593600.033.84236527
TATCGTA1581350.033.8343730
TGAGCGG2195350.033.8177875
GCGGGCT2192600.033.815358