SRR522074_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR522074_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27195847
Sequences flagged as poor quality	0
Sequence length	50
%GC	46

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	1160350	4.2666440945928255	Illumina PCR Primer Index 1 (95% over 24bp)
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT	303632	1.1164645837285376	Illumina Paired End PCR Primer 2 (96% over 29bp)
CTTATACACATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCC	103494	0.3805507510025336	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	79599	0.2926880710867361	No Hit
CATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTG	70661	0.25982275896757323	Illumina Paired End PCR Primer 2 (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	69072	0.25397995510123295	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43031	0.15822636448866623	No Hit
ATACACATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTC	39900	0.14671357726052806	No Hit
ACACATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTT	37501	0.13789237746483868	No Hit
CTTATACACATCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCC	30670	0.11277457179399487	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TTCTGCT	183730	38.724472	44
CTTCTGC	186585	38.1197	43
CGTCTTC	184990	37.946835	40
CCGTCTT	189240	37.1633	39
GCCGTCT	189790	37.083218	38
GTCTTCT	190195	36.95517	41
TCTTCTG	193045	36.32546	42
TATGCCG	195780	36.092747	35
ATGCCGT	195730	35.987766	36
TGCCGTC	195515	35.974445	37
ACCGATA	158785	34.061077	25
CCGATAT	158435	33.989735	26
GACCGAT	208365	33.9337	24
ATATCGT	158040	33.85266	29
ATCGTAT	159475	33.848663	31
GATATCG	159475	33.84682	28
CGATATC	159360	33.842365	27
TATCGTA	158135	33.83437	30
TGAGCGG	219535	33.817787	5
GCGGGCT	219260	33.81535	8