SRR522073_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR522073_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	36242796
Sequences flagged as poor quality	0
Sequence length	50
%GC	45

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	978603	2.700131082601905	Illumina PCR Primer Index 1 (95% over 24bp)
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT	319336	0.8811019988634431	Illumina Paired End PCR Primer 2 (96% over 29bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	112043	0.3091455747509105	No Hit
CTTATACACATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCC	103466	0.2854801820477647	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	98073	0.2705999835112059	No Hit
CATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTG	65237	0.17999990949925607	Illumina Paired End PCR Primer 2 (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59646	0.16457339549630773	No Hit
ATACACATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTC	40472	0.11166908866523431	No Hit
CTTATACACATCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCC	38153	0.10527057570282382	No Hit
ACACATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTT	36449	0.10056895168904739	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTCTTC	172005	37.29412	40
TTCTGCT	176070	37.102993	44
CTTCTGC	178685	36.584393	43
CCGTCTT	176150	36.50987	39
GCCGTCT	176395	36.481586	38
GTCTTCT	179435	35.866943	41
TATGCCG	183730	35.231243	35
ATGCCGT	184205	35.024113	36
TGCCGTC	183840	34.99673	37
TCTTCTG	183435	34.981403	42
ACCGATA	142770	32.93632	25
CCGATAT	142020	32.86017	26
ATATCGT	140140	32.805973	29
TATCGTA	140360	32.779617	30
CGATATC	142420	32.76942	27
ATCGTAT	142340	32.755234	31
GATATCG	142545	32.750202	28
GACCGAT	199650	32.7319	24
AGACCGA	206760	32.510284	23
TGAGCGG	213760	32.47917	5