SRR522072_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR522072_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26586227
Sequences flagged as poor quality	0
Sequence length	50
%GC	45

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	156829	0.5898881402013155	Illumina PCR Primer Index 1 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	69757	0.2623802166437532	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	60380	0.22711007470146102	No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT	46360	0.17437600303345036	Illumina Paired End PCR Primer 2 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37750	0.14199081351408005	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	28365	34.39756	35
CCGTCTT	29575	33.451397	39
GCCGTCT	29695	33.360516	38
GGTATCA	30995	33.309258	1
ATCGTAT	22865	32.319542	31
TATCGTA	22725	32.281628	30
GACCGAT	30410	32.23529	24
ATATCGT	22810	32.167854	29
TCGTATG	30330	32.041813	32
CGTCTTC	30810	32.03865	40
CGTATGC	30450	32.00387	33
ACCGATA	23370	31.920319	25
ATGCCGT	30970	31.775373	36
CCGATAT	23705	31.443985	26
AGACCGA	31650	31.42412	23
TGCCGTC	31470	31.353477	37
GATATCG	23835	31.282772	28
CGATATC	23840	31.257816	27
GCGGGCT	33275	31.017756	8
TGAGCGG	33615	30.830374	5