FastQCFastQC Report
Thu 26 May 2016
SRR522071_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522071_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29632159
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT2379960.8031679365651351Illumina PCR Primer Index 1 (95% over 24bp)
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT548880.18523118750813938Illumina Paired End PCR Primer 2 (96% over 29bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT458370.1546866699790589No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT418040.14107645683191697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG405900.034.93243435
GCCGTCT405850.034.78367638
ATGCCGT416100.033.84879336
TGCCGTC421650.033.3489737
CCGTCTT424650.033.2487139
CCGATAT344200.032.39563426
TATCGTA345800.032.28753730
TCGTATG440000.032.23922332
CGTATGC440350.032.2361333
ACCGATA347300.032.20855725
ATCGTAT349850.032.20372831
ATATCGT345550.032.1978129
CGATATC346950.032.1896427
GATATCG348300.032.06072228
AGACCGA446450.032.05666723
GCGGGCT463600.031.8840588
TGAGCGG474050.031.3432435
CGTCTTC448500.031.27912740
GACCGAT449600.031.25864824
CTGAGCG477850.031.1032334
Produced by FastQC (version 0.11.5)