SRR522070_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR522070_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34538554
Sequences flagged as poor quality	0
Sequence length	50
%GC	44

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	556368	1.610860721036555	Illumina PCR Primer Index 1 (95% over 24bp)
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT	210682	0.6099907946348883	Illumina Paired End PCR Primer 2 (96% over 29bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	115015	0.33300467645518683	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	100313	0.2904377525474865	No Hit
CTTATACACATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCC	73755	0.21354397175979054	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59032	0.17091624623312257	No Hit
CATCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTG	38964	0.11281306102160502	Illumina Paired End PCR Primer 2 (96% over 26bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTCTTC	108120	36.150547	40
CCGTCTT	110095	35.583664	39
GCCGTCT	110300	35.47638	38
TTCTGCT	115405	34.349155	44
TATGCCG	115185	34.126846	35
GTCTTCT	115665	33.750183	41
CTTCTGC	117385	33.737946	43
ATGCCGT	116465	33.72366	36
TGCCGTC	116270	33.662815	37
TCTTCTG	119785	32.615055	42
ACCGATA	86925	31.361217	25
GACCGAT	125265	31.294231	24
CCGATAT	86680	31.238346	26
ATCGTAT	87390	31.207825	31
TATCGTA	86305	31.149988	30
ATATCGT	86010	31.134361	29
GGTATCA	51480	31.128841	1
TCGTATG	126440	31.011057	32
AGACCGA	130220	30.996449	23
CGATATC	87470	30.978588	27