SRR522069_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR522069_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	30746721
Sequences flagged as poor quality	0
Sequence length	50
%GC	45

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	172616	0.5614127112936693	Illumina PCR Primer Index 1 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	64381	0.20939143396786927	No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT	59913	0.19485980309900364	Illumina Paired End PCR Primer 2 (96% over 29bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	56182	0.1827251758000471	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35123	0.11423331938387837	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	35030	34.86677	35
CCGTCTT	36315	34.04972	39
GCCGTCT	36540	33.982517	38
GGTATCA	32930	32.866817	1
GACCGAT	37525	32.842648	24
CGTCTTC	37715	32.826416	40
TCGTATG	37610	32.70994	32
ATGCCGT	37880	32.600048	36
CGTATGC	37805	32.55317	33
ATATCGT	26905	32.49493	29
TATCGTA	26945	32.46468	30
ACCGATA	27280	32.415718	25
ATCGTAT	27200	32.365334	31
TGCCGTC	38210	32.31277	37
AGACCGA	39085	32.01213	23
CGATATC	27780	31.97489	27
CCGATAT	27575	31.965452	26
GATATCG	27890	31.912266	28
GCGGGCT	41165	31.111313	8
TGAGCGG	41740	30.444286	5