FastQCFastQC Report
Thu 26 May 2016
SRR522068_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522068_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29643670
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT1603940.5410733556270192Illumina PCR Primer Index 1 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT445120.15015684630141948No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT409560.1381610306686048Illumina Paired End PCR Primer 2 (96% over 29bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT401650.13549267010461255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG287700.033.86359835
GCCGTCT290400.033.41584838
ATGCCGT298950.032.3760836
TGCCGTC302300.031.98523137
ATCGTAT238200.031.64394231
CCGATAT234950.031.62511426
TATCGTA235600.031.60174830
CCGTCTT307700.031.53958739
ATATCGT236400.031.51390329
CGATATC236000.031.49314927
TCGTATG310150.031.45906332
GATATCG236550.031.45039728
CGTATGC310350.031.4434233
ACCGATA236150.031.42741425
AGACCGA314950.030.97327423
GCGGGCT328700.030.8487078
GACCGAT315300.030.32584824
TGAGCGG336500.030.2771035
CTGAGCG340350.029.8186634
CGGGCTG350050.029.0925799
Produced by FastQC (version 0.11.5)