FastQCFastQC Report
Thu 26 May 2016
SRR522067_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522067_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22027463
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA436390.19811178436663363No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT288950.1311771582592149No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT137300.016.9333821
AGCAGTG158400.014.6772632
GTGGTAT156300.014.4521046
GATCGGA18500.014.4410751
ATCAACG155750.014.30562311
GGTATCA157500.014.2722348
TCAACGC157500.014.18852612
TGGTATC161450.013.9774937
CAACGCA160650.013.89664213
GTATCAA174350.012.9937229
AACGCAG173950.012.83412214
GTACTTT118850.012.8266522
CTCGTAT14650.012.7490612
GAGTACT122850.012.67371920
AGTGGTA183400.012.4963685
TCGTATG15500.012.4752113
ACGCAGA181300.012.27745915
CGCAGAG182900.012.13401616
AGTACTT131750.011.73564321
CAGTGGT198400.011.5848084
Produced by FastQC (version 0.11.5)