##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR522067_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 22027463 Sequences flagged as poor quality 0 Sequence length 50 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.66554527863695 40.0 39.0 40.0 37.0 40.0 2 38.60554436069192 40.0 39.0 40.0 36.0 40.0 3 38.59323468163356 40.0 39.0 40.0 36.0 40.0 4 38.58950828790406 40.0 39.0 40.0 36.0 40.0 5 38.58470655472217 40.0 39.0 40.0 36.0 40.0 6 38.64565778637331 40.0 39.0 40.0 36.0 40.0 7 38.606048594883575 40.0 39.0 40.0 36.0 40.0 8 38.57921327571859 40.0 39.0 40.0 36.0 40.0 9 38.555441495918075 40.0 39.0 40.0 36.0 40.0 10 38.529898200260284 40.0 39.0 40.0 36.0 40.0 11 38.574939746806066 40.0 39.0 40.0 36.0 40.0 12 38.526685074899454 40.0 39.0 40.0 36.0 40.0 13 38.49512933014574 40.0 39.0 40.0 35.0 40.0 14 38.4560168368005 40.0 38.0 40.0 35.0 40.0 15 38.42450807884685 40.0 38.0 40.0 35.0 40.0 16 38.3919860403352 40.0 38.0 40.0 35.0 40.0 17 38.32550294148718 40.0 38.0 40.0 35.0 40.0 18 38.271192919493274 40.0 38.0 40.0 35.0 40.0 19 38.223624300265534 40.0 38.0 40.0 35.0 40.0 20 38.163255477945874 40.0 38.0 40.0 35.0 40.0 21 38.03765799084534 39.0 38.0 40.0 35.0 40.0 22 37.92698768805105 39.0 38.0 40.0 34.0 40.0 23 37.8583283966928 39.0 38.0 40.0 34.0 40.0 24 37.98791535820535 40.0 38.0 40.0 35.0 40.0 25 37.884382509234044 40.0 38.0 40.0 34.0 40.0 26 37.804775520449176 39.0 38.0 40.0 34.0 40.0 27 37.721074823732536 39.0 38.0 40.0 33.0 40.0 28 37.75979694075527 39.0 38.0 40.0 34.0 40.0 29 37.71573353681266 39.0 38.0 40.0 34.0 40.0 30 37.64074905040131 39.0 38.0 40.0 33.0 40.0 31 37.64577200742546 39.0 38.0 40.0 34.0 40.0 32 37.56728988717403 39.0 38.0 40.0 33.0 40.0 33 37.47174729109748 39.0 38.0 40.0 33.0 40.0 34 37.40957730810852 39.0 38.0 40.0 33.0 40.0 35 37.28632593776233 39.0 38.0 40.0 33.0 40.0 36 37.1142859257101 39.0 38.0 40.0 33.0 40.0 37 36.982440192953675 39.0 37.0 40.0 33.0 40.0 38 36.88610336106341 39.0 37.0 40.0 32.0 40.0 39 36.77081550426393 39.0 37.0 40.0 32.0 40.0 40 36.61593044101357 39.0 36.0 40.0 32.0 40.0 41 36.344310327521605 39.0 36.0 40.0 31.0 40.0 42 36.21031314409653 39.0 36.0 40.0 31.0 40.0 43 36.065616226435154 39.0 36.0 40.0 31.0 40.0 44 35.905634116829525 39.0 36.0 40.0 30.0 40.0 45 35.70585459614664 39.0 36.0 40.0 30.0 40.0 46 35.36162421428196 39.0 35.0 40.0 29.0 40.0 47 35.130990663790925 39.0 35.0 40.0 29.0 40.0 48 34.93293131396929 38.0 35.0 40.0 28.0 40.0 49 34.75331548621827 38.0 35.0 40.0 27.0 40.0 50 34.56270483804694 38.0 34.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1 1 -0.14146375991140303 1 2 -0.1516548250152283 1 3 -0.16534457750887555 1 4 -0.16865894485380295 1 5 -0.1599357853866934 1 6 -0.18458855709565114 1 7 -0.19316561006082367 1 8 -0.18555055235896845 1 9 -0.20267614985725402 1 10 -0.18479149112734916 1 11 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-0.21526346270411523 119 48 -0.23883785162096416 119 49 -0.3793766563745038 119 50 -0.3583980311934596 120 1 -0.18369470615470362 120 2 -0.1827334788795767 120 3 -0.175035488327012 120 4 -0.2132542941029527 120 5 -0.1808798391780826 120 6 -0.18115088864096407 120 7 -0.19015440678145978 120 8 -0.20356626830222524 120 9 -0.1844076193637605 120 10 -0.19975945773767734 120 11 -0.17305370055219527 120 12 -0.18486554077722417 120 13 -0.20174709689722903 120 14 -0.19141157709176326 120 15 -0.1758443956622031 120 16 -0.18365264680156912 120 17 -0.20365473536558198 120 18 -0.18286020278697634 120 19 -0.19890647453378563 120 20 -0.17913351514550868 120 21 -0.22440866245529634 120 22 -0.186962856033702 120 23 -0.2553736860723461 120 24 -0.20755091868135622 120 25 -0.20474761147423237 120 26 -0.1688379345298756 120 27 -0.267827698884588 120 28 -0.20302978580109254 120 29 -0.19922715197809993 120 30 -0.3049185103608849 120 31 -0.20642406526307155 120 32 -0.2868849576948662 120 33 -0.19975715996721988 120 34 -0.1271847186303674 120 35 -0.1301127306545169 120 36 -0.06972666814557016 120 37 -0.08779007629723168 120 38 -0.09304936837062172 120 39 -0.11408711379234404 120 40 -0.1330025830876167 120 41 0.008245193905715098 120 42 -0.04514762004147599 120 43 -0.05922240882544827 120 44 -0.019231611285981387 120 45 -0.002900749647039902 120 46 -0.004833276432648859 120 47 -0.121033113543497 120 48 -0.21641205359558313 120 49 -0.06370026987931254 120 50 -0.1416950711399778 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 21905.0 3 763.0 4 1156.0 5 1310.0 6 2199.0 7 3746.0 8 4076.0 9 5044.0 10 6992.0 11 9519.0 12 10653.0 13 14582.0 14 18583.0 15 21791.0 16 24186.0 17 27711.0 18 32535.0 19 40743.0 20 47134.0 21 43462.0 22 53845.0 23 67153.0 24 80716.0 25 93567.0 26 121055.0 27 148736.0 28 165181.0 29 184617.0 30 217435.0 31 261018.0 32 311635.0 33 396312.0 34 543739.0 35 786675.0 36 1214968.0 37 2117118.0 38 4103807.0 39 1.0739764E7 40 82032.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 20.104808572800266 28.89970156674607 26.585830065240224 24.409659795213436 2 23.446258687837478 28.595684453184806 27.18621342895633 20.771843430021384 3 22.78370733045373 27.266565375196638 27.25637781467111 22.693349479678517 4 23.816738209466095 26.656196058595093 26.686848827281906 22.840216904656906 5 24.355308118081933 27.733703091284152 26.918949019045975 20.992039771587937 6 24.27189230596998 26.91500447487275 25.700278770453693 23.112824448703577 7 23.783401656377766 26.85236152706283 27.220529209378313 22.14370760718109 8 23.3022114258006 26.84871153795605 27.129297640858596 22.71977939538475 9 22.99741463644724 26.58981199968421 27.51202442151418 22.90074894235437 10 23.041264443390507 27.25738320386692 26.725619741138594 22.975732611603977 11 23.225448159872066 27.166769046439892 26.758560438848537 22.8492223548395 12 23.09346291944742 26.979575450881477 27.038374777885227 22.888586851785885 13 23.040106797591715 27.05869940628206 26.90637591809824 22.994817878027987 14 23.120106931969424 27.19266853382071 26.97728285822112 22.709941675988745 15 22.952929259261495 27.098318131325428 26.95195084427108 22.996801765141996 16 23.02044951794948 26.936942306973798 27.010536801264855 23.032071373811863 17 23.391322913582922 26.842133385946443 27.02774259568612 22.738801104784514 18 23.009072810609194 26.778358451901614 27.006160446166678 23.206408291322518 19 23.18722314957469 26.940814745665442 26.98932691431601 22.882635190443857 20 23.37847531511005 26.652783391351058 27.108337442219288 22.8604038513196 21 23.10014548656829 26.940083839886597 26.96980128850971 22.9899693850354 22 23.41282624738871 26.678391730756378 26.85006838737982 23.058713634475083 23 23.16325624375461 26.812929095240847 27.155283576538004 22.86853108446654 24 23.122627887404843 26.88390491329262 26.92737368791263 23.06609351138991 25 23.25399910980777 26.6845952465951 26.87097158155988 23.19043406203725 26 23.330787909002737 26.795944965954327 27.052736167608582 22.820530957434354 27 23.329318949763834 26.633927337007083 26.980619418763848 23.056134294465235 28 23.40660165099213 26.728253017558668 26.848073343417422 23.01707198803178 29 23.36576752138073 26.834457028241854 26.905984785979125 22.893790664398296 30 23.284315842072235 26.723760767638804 26.90920918037365 23.082714209915313 31 23.36093431027491 26.75912873481302 26.825004878662117 23.054932076249955 32 23.41432149677191 26.766065232121015 26.822472305195266 22.99714096591181 33 23.268408614124596 26.829197336572363 26.70624724904407 23.196146800258973 34 23.35920441801739 26.865434911085018 26.724390552941145 23.05097011795644 35 23.40913112663115 26.850328074971692 26.72635355771382 23.01418724068334 36 23.307765309186564 26.821491100193622 26.75277762226373 23.11796596835608 37 23.383264792800333 26.849865107541827 26.689159193820473 23.077710905837368 38 23.454815382274464 26.813086607963683 26.726471917988643 23.005626091773212 39 23.362455239897784 26.767050201312507 26.700511167412554 23.16998339137715 40 23.41257399904093 26.809021390483966 26.66785675175775 23.110547858717354 41 23.416686382759476 26.847054711191625 26.681175892775364 23.055083013273535 42 23.353849112087794 26.796504218580207 26.636566150033307 23.21308051929869 43 23.436649985101795 26.802494169605318 26.608267399189796 23.152588446103096 44 23.401284102725306 26.827401443824233 26.663118411487723 23.108196041962735 45 23.332270267817414 26.750276989480387 26.63326847677883 23.284184265923376 46 23.468627820712086 26.81727919793126 26.514467065902075 23.19962591545458 47 23.4846185698377 26.860835100262797 26.551539887513197 23.103006442386302 48 23.437430030551383 26.716379188799692 26.514839843846982 23.331350936801943 49 23.504930742268574 26.778421501867804 26.405634561278863 23.31101319458476 50 23.43408135003372 26.849926635237836 26.49813477603622 23.217857238692222 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 46352.0 1 25870.5 2 5389.0 3 4229.5 4 3070.0 5 3002.0 6 2934.0 7 3416.5 8 3899.0 9 4923.5 10 5948.0 11 8432.0 12 10916.0 13 16502.5 14 22089.0 15 31716.0 16 41343.0 17 58522.0 18 75701.0 19 102505.5 20 129310.0 21 169448.5 22 209587.0 23 268156.5 24 326726.0 25 355400.0 26 384074.0 27 436782.5 28 489491.0 29 544083.5 30 598676.0 31 670536.5 32 742397.0 33 821229.0 34 900061.0 35 977211.5 36 1054362.0 37 1113423.0 38 1172484.0 39 1216115.5 40 1259747.0 41 1292616.5 42 1325486.0 43 1355298.0 44 1385110.0 45 1389362.5 46 1393615.0 47 1357072.0 48 1320529.0 49 1293980.0 50 1267431.0 51 1242707.5 52 1217984.0 53 1186478.0 54 1154972.0 55 1116574.0 56 1078176.0 57 1032008.0 58 985840.0 59 945870.0 60 905900.0 61 825234.0 62 744568.0 63 646860.0 64 549152.0 65 473509.0 66 397866.0 67 344112.5 68 290359.0 69 243735.5 70 197112.0 71 162147.0 72 127182.0 73 104714.0 74 82246.0 75 64521.5 76 46797.0 77 37280.0 78 27763.0 79 22212.5 80 16662.0 81 13541.5 82 10421.0 83 8708.0 84 6995.0 85 5831.5 86 4668.0 87 3914.0 88 3160.0 89 2243.0 90 1326.0 91 876.0 92 426.0 93 309.5 94 193.0 95 164.5 96 136.0 97 222.0 98 308.0 99 416.0 100 524.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4750342788000597 2 0.0018386139157287428 3 0.0027238724677462857 4 4.0404108271569904E-4 5 3.6318299569950476E-4 6 9.533553637112E-5 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 3.177851212370667E-5 23 0.014595416639673847 24 7.127466290602781E-4 25 0.03404840584682857 26 0.06998536327129457 27 0.07918297263738452 28 0.09615269811144388 29 0.10631274241613754 30 0.11647732650827741 31 0.1298560801123579 32 0.12110336991599986 33 0.12843966642912985 34 0.10780633248595173 35 0.07485201541366793 36 0.09801855075185009 37 0.08880278223597515 38 0.11016702195799852 39 0.12186151441952257 40 0.13181726828913526 41 0.12560683906267373 42 0.1314177669938658 43 0.1431167992428361 44 0.17791880980574112 45 0.2560984894175058 46 0.3025859128670424 47 0.33008794521638735 48 0.34465158334393753 49 0.3537765561108876 50 0.3520695960310999 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2.2027463E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.01102713389873 #Duplication Level Percentage of deduplicated Percentage of total 1 84.7794991869464 48.33366328545346 2 8.966116895453704 10.223350672248376 3 2.39312447502943 4.093034531420999 4 1.074962138350045 2.4513878254955284 5 0.6052195840958883 1.7252095065428796 6 0.4069698952563319 1.39210630446832 7 0.2859007553961273 1.140964700644353 8 0.21075368154379912 0.9612227085650066 9 0.16159410327238624 0.8291381225706039 >10 0.9157257273290518 9.15369776219794 >50 0.0637282151781304 2.64867332229289 >100 0.13288258515981127 15.32131431982644 >500 0.0032522802478014375 1.129812291123138 >1k 2.5458551452691016E-4 0.2678546399431379 >5k 0.0 0.0 >10k+ 1.5891226563040743E-5 0.3285700072067544 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 43639 0.19811178436663363 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 28895 0.1311771582592149 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.0858087016194286E-5 9.07957489248762E-6 0.0 0.0 0.0 2 4.5397874462438095E-5 9.07957489248762E-6 0.0 4.53978744624381E-6 0.0 3 4.5397874462438095E-5 9.07957489248762E-6 0.0 4.53978744624381E-6 0.0 4 4.5397874462438095E-5 9.07957489248762E-6 0.0 1.3619362338731428E-5 0.0 5 4.5397874462438095E-5 1.3619362338731428E-5 0.0 1.3619362338731428E-5 0.0 6 4.9937661908681904E-5 1.815914978497524E-5 0.0 1.3619362338731428E-5 0.0 7 4.9937661908681904E-5 2.7238724677462856E-5 0.0 1.815914978497524E-5 0.0 8 5.447744935492571E-5 3.177851212370667E-5 0.0 2.2698937231219047E-5 0.0 9 5.447744935492571E-5 3.177851212370667E-5 0.0 3.177851212370667E-5 0.0 10 4.5397874462438094E-4 3.177851212370667E-5 0.0 3.177851212370667E-5 0.0 11 4.857572567480876E-4 3.177851212370667E-5 0.0 3.631829956995048E-5 0.0 12 4.902970441943315E-4 3.631829956995048E-5 0.0 4.0858087016194286E-5 0.0 13 4.948368316405752E-4 3.631829956995048E-5 0.0 4.0858087016194286E-5 4.53978744624381E-6 14 5.084561939793067E-4 3.631829956995048E-5 0.0 4.0858087016194286E-5 4.53978744624381E-6 15 5.175357688717943E-4 3.631829956995048E-5 0.0 4.5397874462438095E-5 4.53978744624381E-6 16 5.266153437642819E-4 3.631829956995048E-5 0.0 5.447744935492571E-5 4.53978744624381E-6 17 5.493142809955009E-4 3.631829956995048E-5 0.0 5.447744935492571E-5 4.53978744624381E-6 18 5.674734307804762E-4 3.631829956995048E-5 0.0 6.809681169365715E-5 4.53978744624381E-6 19 5.94712155457939E-4 4.5397874462438095E-5 0.0 6.809681169365715E-5 4.53978744624381E-6 20 6.219508801354019E-4 4.5397874462438095E-5 0.0 6.809681169365715E-5 4.53978744624381E-6 21 6.582691797053523E-4 4.5397874462438095E-5 0.0 7.263659913990095E-5 4.53978744624381E-6 22 7.082068416140343E-4 4.5397874462438095E-5 0.0 7.263659913990095E-5 4.53978744624381E-6 23 7.808434407539352E-4 4.9937661908681904E-5 0.0 7.717638658614476E-5 4.53978744624381E-6 24 8.398606775551047E-4 5.447744935492571E-5 0.0 8.171617403238857E-5 4.53978744624381E-6 25 8.80718764571299E-4 5.901723680116952E-5 0.0 8.171617403238857E-5 4.53978744624381E-6 26 9.215768515874934E-4 6.355702424741334E-5 0.0 8.625596147863238E-5 4.53978744624381E-6 27 0.0010305317502973448 6.355702424741334E-5 0.0 9.079574892487619E-5 4.53978744624381E-6 28 0.0010850091996522705 7.263659913990095E-5 0.0 9.533553637112E-5 4.53978744624381E-6 29 0.0012393619728245599 7.717638658614476E-5 0.0 9.987532381736381E-5 4.53978744624381E-6 30 0.0014073341083355808 8.171617403238857E-5 0.0 9.987532381736381E-5 4.53978744624381E-6 31 0.0017024202923414285 8.171617403238857E-5 0.0 1.0441511126360762E-4 4.53978744624381E-6 32 0.002265353935675661 8.171617403238857E-5 0.0 1.0441511126360762E-4 4.53978744624381E-6 33 0.005361488974013939 8.171617403238857E-5 0.0 1.0441511126360762E-4 4.53978744624381E-6 34 0.005797308568853345 8.171617403238857E-5 0.0 1.0441511126360762E-4 4.53978744624381E-6 35 0.0059743602792568535 8.171617403238857E-5 0.0 1.0895489870985143E-4 4.53978744624381E-6 36 0.006187730289230312 8.171617403238857E-5 0.0 1.0895489870985143E-4 4.53978744624381E-6 37 0.006555453072376061 8.625596147863238E-5 0.0 1.0895489870985143E-4 4.53978744624381E-6 38 0.006895937130844346 8.625596147863238E-5 0.0 1.1349468615609523E-4 4.53978744624381E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGCAGT 13730 0.0 16.933382 1 AGCAGTG 15840 0.0 14.677263 2 GTGGTAT 15630 0.0 14.452104 6 GATCGGA 1850 0.0 14.441075 1 ATCAACG 15575 0.0 14.305623 11 GGTATCA 15750 0.0 14.272234 8 TCAACGC 15750 0.0 14.188526 12 TGGTATC 16145 0.0 13.977493 7 CAACGCA 16065 0.0 13.896642 13 GTATCAA 17435 0.0 12.993722 9 AACGCAG 17395 0.0 12.834122 14 GTACTTT 11885 0.0 12.82665 22 CTCGTAT 1465 0.0 12.74906 12 GAGTACT 12285 0.0 12.673719 20 AGTGGTA 18340 0.0 12.496368 5 TCGTATG 1550 0.0 12.47521 13 ACGCAGA 18130 0.0 12.277459 15 CGCAGAG 18290 0.0 12.134016 16 AGTACTT 13175 0.0 11.735643 21 CAGTGGT 19840 0.0 11.584808 4 >>END_MODULE