FastQCFastQC Report
Thu 26 May 2016
SRR522066_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522066_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69105238
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1787830.2587112137577762No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT925480.13392327800101056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT439300.015.7596371
TCAACGC428500.015.34385212
ATCAACG430700.015.31661911
CAACGCA435250.015.12610313
GGTATCA466750.014.2373918
GTGGTAT468650.014.2337726
AGCAGTG492200.014.01122952
AACGCAG475250.013.8878614
TGGTATC479250.013.8568717
ACGCAGA490650.013.46988715
CGCAGAG489750.013.42281216
GTACTTT350200.013.37913622
GTATCAA508750.013.17449
GAGTACT369800.012.95938820
AGTGGTA546200.012.4624375
AGAGTAC544150.012.09089819
AGTACTT394500.011.9366421
GCAGAGT591000.011.32806517
CAGTGGT602150.011.3080734
TATCAAC599300.011.30492610
Produced by FastQC (version 0.11.5)