FastQCFastQC Report
Thu 26 May 2016
SRR522065_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522065_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72603242
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3979260.5480829630169959No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1227060.1690089817201276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT479150.017.7684141
TCAACGC476000.017.18381312
ATCAACG480350.017.028211
CAACGCA482800.016.8826313
GGTATCA522450.015.6981868
GTGGTAT526050.015.6394916
AGCAGTG543900.015.564252
GTACTTT395350.015.52387722
TGGTATC535200.015.3483747
AACGCAG535650.015.31039614
CGCAGAG541250.015.08648516
GAGTACT416850.014.94235720
ACGCAGA554400.014.81987615
GTATCAA568350.014.4884599
AGTGGTA598950.013.9378935
AGTACTT451450.013.71977121
AGAGTAC606050.013.48002619
CAGTGGT653150.012.7881384
GCAGAGT650000.012.68813617
CAGAGTA648750.012.67192318
Produced by FastQC (version 0.11.5)