FastQCFastQC Report
Thu 26 May 2016
SRR522064_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522064_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41550639
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1158830.2788958311808394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC219700.011.1952919
CAACGCA222700.011.14326710
AAGCAGT224300.011.0707741
ATCAACG224900.010.96588
GGTATCA244300.010.2369175
GTGGTAT247200.010.08124353
AACGCAG250750.010.0107911
TGGTATC254850.09.8217714
AGCAGTG258250.09.6552212
ACGCAGA258450.09.6444412
CGCAGAG257300.09.62769313
GTATCAA274450.09.2806316
AGTGGTA285150.08.9836382
GAGTACA233250.08.67550417
AGAGTAC296550.08.38867216
CAGTGGT319800.08.2810491
TACATGG239700.08.2315520
ACATGGG243750.08.12185321
GCAGAGT321350.07.873034514
CAGAGTA326000.07.779294515
Produced by FastQC (version 0.11.5)