FastQCFastQC Report
Thu 26 May 2016
SRR522063_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522063_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20963238
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1600170.7633219639065301No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT300270.143236459940015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT128100.017.1534041
AGCAGTG147000.015.1130532
ATCAACG149550.014.28206311
TCAACGC151050.014.16929812
GTGGTAT153500.014.0716786
GGTATCA152650.013.97753058
CAACGCA152650.013.9343813
TGGTATC157950.013.5778497
AACGCAG168000.012.67428814
GTATCAA169400.012.6603739
CGCAGAG170500.012.50109716
AGTGGTA173750.012.4317895
GAGTACT125550.012.27887420
ACGCAGA175050.012.22643115
GTACTTT126250.011.95330122
CAGTGGT183100.011.9293124
AGAGTAC186100.011.46794319
AGTACTT136750.011.1780921
GCAGAGT201950.010.772614517
CAGAGTA201100.010.71987718
Produced by FastQC (version 0.11.5)