##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522063_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20963238
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.81225901265825	40.0	39.0	40.0	38.0	40.0
2	38.77317688231179	40.0	39.0	40.0	38.0	40.0
3	38.762250182915444	40.0	39.0	40.0	38.0	40.0
4	38.75422852137633	40.0	39.0	40.0	38.0	40.0
5	38.750425578338614	40.0	39.0	40.0	38.0	40.0
6	38.80294155893283	40.0	39.0	40.0	38.0	40.0
7	38.76883036866728	40.0	39.0	40.0	37.0	40.0
8	38.745010145856284	40.0	39.0	40.0	37.0	40.0
9	38.72134490864436	40.0	39.0	40.0	37.0	40.0
10	38.69679750809488	40.0	39.0	40.0	37.0	40.0
11	38.744714008398894	40.0	39.0	40.0	36.0	40.0
12	38.70313407690167	40.0	39.0	40.0	36.0	40.0
13	38.67024898539052	40.0	39.0	40.0	36.0	40.0
14	38.63951656704942	40.0	39.0	40.0	36.0	40.0
15	38.607061227850394	40.0	39.0	40.0	36.0	40.0
16	38.566192589141046	40.0	39.0	40.0	36.0	40.0
17	38.5079200073958	40.0	39.0	40.0	36.0	40.0
18	38.45327835327729	40.0	38.0	40.0	35.0	40.0
19	38.41233921019263	40.0	38.0	40.0	35.0	40.0
20	38.3565294636258	40.0	38.0	40.0	35.0	40.0
21	38.226185048321256	40.0	38.0	40.0	35.0	40.0
22	38.12560120721808	40.0	38.0	40.0	35.0	40.0
23	38.048180247727	40.0	38.0	40.0	35.0	40.0
24	38.16502608041754	40.0	38.0	40.0	35.0	40.0
25	38.071679336942125	40.0	38.0	40.0	35.0	40.0
26	37.99699306948669	40.0	38.0	40.0	35.0	40.0
27	37.90801506904611	40.0	38.0	40.0	35.0	40.0
28	37.97043762991194	40.0	38.0	40.0	35.0	40.0
29	37.92100757526104	40.0	38.0	40.0	35.0	40.0
30	37.83264708438649	40.0	38.0	40.0	34.0	40.0
31	37.84812742191831	40.0	38.0	40.0	35.0	40.0
32	37.7728506445426	40.0	38.0	40.0	34.0	40.0
33	37.68198476781116	40.0	38.0	40.0	34.0	40.0
34	37.63236395064541	40.0	38.0	40.0	34.0	40.0
35	37.5307725361893	40.0	38.0	40.0	33.0	40.0
36	37.38993990336798	39.0	38.0	40.0	33.0	40.0
37	37.28189504884694	39.0	38.0	40.0	33.0	40.0
38	37.17914126624904	39.0	38.0	40.0	33.0	40.0
39	37.06566094417284	39.0	38.0	40.0	33.0	40.0
40	36.944181571568286	39.0	37.0	40.0	33.0	40.0
41	36.678764893095234	39.0	37.0	40.0	32.0	40.0
42	36.54898880602319	39.0	37.0	40.0	32.0	40.0
43	36.425055280105106	39.0	36.0	40.0	31.0	40.0
44	36.27570583323053	39.0	36.0	40.0	31.0	40.0
45	36.078775282711575	39.0	36.0	40.0	31.0	40.0
46	35.6832947276561	39.0	36.0	40.0	30.0	40.0
47	35.50942907770259	39.0	36.0	40.0	30.0	40.0
48	35.33108363316774	39.0	35.0	40.0	29.0	40.0
49	35.14959067869191	39.0	35.0	40.0	29.0	40.0
50	35.001370112765976	39.0	35.0	40.0	29.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1	1	-0.1531434087832082
1	2	-0.16234540020291632
1	3	-0.1790347308780369
1	4	-0.1550548179322604
1	5	-0.15592984013231614
1	6	-0.16710511966250152
1	7	-0.169263885964142
1	8	-0.18628428110700668
1	9	-0.19521116124417404
1	10	-0.18581853957998362
1	11	-0.16883178220074058
1	12	-0.19006761848499565
1	13	-0.1761988574211486
1	14	-0.21505313008689342
1	15	-0.18481998207118977
1	16	-0.19239937409432173
1	17	-0.17880247513977565
1	18	-0.16044667379575372
1	19	-0.2046196341140316
1	20	-0.20381487083637495
1	21	-0.2048652458450917
1	22	-0.23694481144892876
1	23	-0.23596841992078765
1	24	-0.17400055045545315
1	25	-0.19900302787605284
1	26	-0.1969729254191961
1	27	-0.19856830737416686
1	28	-0.26107017843040836
1	29	-0.26061641323552465
1	30	-0.20030183169259175
1	31	-0.1663561952817929
1	32	-0.12310178651711112
1	33	-0.1935112320094916
1	34	-0.1554985814582679
1	35	-0.11667144712447453
1	36	-0.04800460076842228
1	37	-0.07146626980179605
1	38	-0.05744353835613225
1	39	-0.11657410281394931
1	40	-0.07223982501505333
1	41	-0.07792034179627905
1	42	-0.061413089958250566
1	43	-0.05828119839828361
1	44	-0.0477536446392719
1	45	-0.012396247239934155
1	46	-0.21298676837295005
1	47	-0.20661548753913905
1	48	-0.12821729528283043
1	49	-0.32498897949228933
1	50	-0.2813501024843674
2	1	-0.10253788315331036
2	2	-0.11636027602511234
2	3	-0.13675522702542509
2	4	-0.10929264043932108
2	5	-0.09944601611346116
2	6	-0.13583427386859626
2	7	-0.12695588631055443
2	8	-0.1351462845626017
2	9	-0.1490311551961696
2	10	-0.1290813209608075
2	11	-0.14436037930884993
2	12	-0.15464807308327977
2	13	-0.14327796784916558
2	14	-0.17350186634375575
2	15	-0.13722127048702504
2	16	-0.15707748512085828
2	17	-0.1806875078673542
2	18	-0.15067801406583214
2	19	-0.18757889160877994
2	20	-0.1810030441257311
2	21	-0.18133468607600634
2	22	-0.17010111588630394
2	23	-0.18273958200794738
2	24	-0.17490825833638013
2	25	-0.1508236509666574
2	26	-0.11391499234031954
2	27	-0.19213862537716864
2	28	-0.17092378809797282
2	29	-0.2036781268249399
2	30	-0.17712790042202897
2	31	-0.19735148870842778
2	32	-0.20801541990210382
2	33	-0.16220433547804447
2	34	-0.20423332226106794
2	35	-0.19069380284090443
2	36	-0.22361030386327485
2	37	-0.1814562587502877
2	38	-0.17220847537838324
2	39	-0.20995507078283993
2	40	-0.21104877861881732
2	41	-0.23635719804216393
2	42	-0.14242393945560394
2	43	-0.12476631334791222
2	44	-0.1616914175169839
2	45	-0.13127091763681875
2	46	-0.2279739878540994
2	47	-0.2149250304279562
2	48	-0.132589220449006
2	49	-0.19144624765562668
2	50	-0.2302611116190505
3	1	-0.10525919561228392
3	2	-0.11282823125732477
3	3	-0.11015685958204813
3	4	-0.1160130641273085
3	5	-0.10518312971881016
3	6	-0.10186244825911928
3	7	-0.11247687718535815
3	8	-0.11999339652996355
3	9	-0.11821082421528928
3	10	-0.11943960679317911
3	11	-0.12501724925294155
3	12	-0.11969808879676691
3	13	-0.11295700480332727
3	14	-0.12151500104070578
3	15	-0.10728905093080243
3	16	-0.11787216687793745
3	17	-0.13517206459015796
3	18	-0.12915714150278745
3	19	-0.1448857817877709
3	20	-0.15010185995745218
3	21	-0.18100757181974103
3	22	-0.15500514349401584
3	23	-0.16427121293477853
3	24	-0.12341292014239258
3	25	-0.08559848063934794
3	26	-0.11974824277355367
3	27	-0.13425760819994537
3	28	-0.21709513722176155
3	29	-0.17095374308201627
3	30	-0.16883812887560623
3	31	-0.16572761805460345
3	32	-0.15910374474277944
3	33	-0.19371509695768907
3	34	-0.19470214725069468
3	35	-0.18925693594353987
3	36	-0.14827212829963798
3	37	-0.127051081435269
3	38	-0.1681761699926838
3	39	-0.21117111523021492
3	40	-0.19036634564884025
3	41	-0.11521105782777141
3	42	-0.09454954473438448
3	43	-0.051404099847374596
3	44	-0.07058022361948701
3	45	-0.19146628054389936
3	46	-0.20681580040107406
3	47	-0.23906276018234962
3	48	-0.20038474317021127
3	49	-0.2699096263472569
3	50	-0.2368646848250222
4	1	-0.08342494128509514
4	2	-0.12491135436640377
4	3	-0.11257194295862405
4	4	-0.1257304556503911
4	5	-0.10721797272181988
4	6	-0.10329920026605066
4	7	-0.13240465906513776
4	8	-0.11411282722431793
4	9	-0.13003800478582406
4	10	-0.13583777735903624
4	11	-0.1259673111503048
4	12	-0.13599547949253576
4	13	-0.12208077733725275
4	14	-0.23102634484524742
4	15	-0.14493009283813763
4	16	-0.1268613000608525
4	17	-0.12942569542055082
4	18	-0.14291008005054096
4	19	-0.16121283945797416
4	20	-0.1855475856851143
4	21	-0.15275562821366861
4	22	-0.1963648987219102
4	23	-0.16328345840770453
4	24	-0.12697367579017538
4	25	-0.17666966364968317
4	26	-0.13183288317068786
4	27	-0.2002364802032801
4	28	-0.15386966390374823
4	29	-0.28197159421561935
4	30	-0.24781624654720957
4	31	-0.20163947064575893
4	32	-0.20857687844253547
4	33	-0.19253911885390806
4	34	-0.18451978679447478
4	35	-0.17457186345470177
4	36	-0.1370372490733729
4	37	-0.18376379539883914
4	38	-0.12415181735961056
4	39	-0.14935903566873776
4	40	-0.16120343027726136
4	41	-0.11419970547850511
4	42	-0.12591600158407346
4	43	-0.07726439818453912
4	44	-0.10266743222631192
4	45	-0.12113953974733249
4	46	-0.18668581992297817
4	47	-0.2136543341866357
4	48	-0.1969723223946076
4	49	-0.24677190179062336
4	50	-0.19586292972202557
5	1	-0.08328392767494108
5	2	-0.10061957465786264
5	3	-0.09320244158350732
5	4	-0.0806692911901905
5	5	-0.08466788541222314
5	6	-0.10923782075622768
5	7	-0.09181064296763708
5	8	-0.09343554526054021
5	9	-0.11424844758298747
5	10	-0.09026681031479455
5	11	-0.11155428103247544
5	12	-0.09419603831928924
5	13	-0.0908757070710493
5	14	-0.11914226356274327
5	15	-0.09796299488037619
5	16	-0.11591226558417844
5	17	-0.1270949264362926
5	18	-0.13660391568290464
5	19	-0.12931719578073597
5	20	-0.1291801728776747
5	21	-0.12735067219407625
5	22	-0.10032667876122048
5	23	-0.12996694503902972
5	24	-0.11106235674395748
5	25	-0.07113605499376519
5	26	-0.10402807678249815
5	27	-0.08105713854089203
5	28	-0.08712796171577253
5	29	-0.1479703413566611
5	30	-0.1049770845998026
5	31	-0.06801567950128629
5	32	-0.1371326531465087
5	33	-0.07439902912247476
5	34	-0.13969288078627784
5	35	-0.09765101713672664
5	36	-0.11154590053570956
5	37	-0.14526936393373546
5	38	-0.1201569126032993
5	39	-0.14275226209002057
5	40	-0.1289592282963099
5	41	-0.05984938322522737
5	42	-0.04214606016268618
5	43	-0.09365839388649988
5	44	-0.07446029654264663
5	45	0.0094606935888919
5	46	-0.06987182657793767
5	47	-0.09765591334672763
5	48	-0.032246451925367126
5	49	-0.04830091282924798
5	50	-0.1872465361676916
6	1	-0.044480003944450175
6	2	-0.04161106480328414
6	3	-0.03882757406730519
6	4	-0.044410460744444435
6	5	-0.035526819897839346
6	6	-0.04602019635804311
6	7	-0.035584402381772406
6	8	-0.042938395352052794
6	9	-0.05829684411495606
6	10	-0.027866092412971
6	11	-0.017004715090216393
6	12	-0.02355219257162844
6	13	-0.051524207689027435
6	14	-0.06534576538454218
6	15	-0.041302176303325666
6	16	-0.06045715047081757
6	17	-0.0641613549066733
6	18	-0.07630750704230138
6	19	-0.08622904778905394
6	20	-0.0633051727277163
6	21	-0.04799007336133343
6	22	-0.05438345597462302
6	23	-0.09819567292883136
6	24	-0.027549071543596426
6	25	-0.05335296636728515
6	26	0.007904799140874275
6	27	-0.0401716533108285
6	28	-0.08774144185476018
6	29	-0.08568709684110587
6	30	-0.11866800720932957
6	31	-0.05082295988779606
6	32	-0.04843374344257256
6	33	-0.06975080030102987
6	34	-0.02993363308289787
6	35	-0.014274524193751859
6	36	0.007544038922944196
6	37	-0.019903758840051466
6	38	0.049176585939690654
6	39	-0.007791966623820201
6	40	0.030076986071030376
6	41	0.0717778691377049
6	42	-0.025702286377317307
6	43	0.07831491779815991
6	44	0.05783620950267476
6	45	0.08320860234055516
6	46	-0.022202695239748493
6	47	-0.07240805502772218
6	48	0.06842478814878206
6	49	0.016517723719751132
6	50	-0.045836494705852715
7	1	-0.07423723081165434
7	2	-0.07498879594501773
7	3	-0.05742388442174473
7	4	-0.06374249928152409
7	5	-0.0730096058660834
7	6	-0.05701610723986761
7	7	-0.0737662625167772
7	8	-0.08173486276270836
7	9	-0.07735378985455554
7	10	-0.07643279149028359
7	11	-0.08512545144574091
7	12	-0.07339848546762084
7	13	-0.09334181041570844
7	14	-0.07815013891250544
7	15	-0.0928432249602551
7	16	-0.06354404303896644
7	17	-0.09339823267005443
7	18	-0.07749954091448785
7	19	-0.08251723000127242
7	20	-0.09500755608548417
7	21	-0.10997387645888068
7	22	-0.07846444316384549
7	23	-0.07874283064660403
7	24	-0.08307474081909305
7	25	-0.07888539962828389
7	26	-0.04953652766408112
7	27	-0.1362766905625108
7	28	-0.11125688583245363
7	29	-0.08774099037808725
7	30	-0.08023958429397737
7	31	-0.0774155051529064
7	32	-0.09557631892243279
7	33	-0.08313749461102304
7	34	-0.07442888485004318
7	35	-0.029350530138003705
7	36	-0.023248980327444713
7	37	-0.01945103504250767
7	38	-0.01190951529511608
7	39	-0.02518356827133772
7	40	0.03811165866922295
7	41	0.015060189267948942
7	42	0.01911658394923421
7	43	0.029154346264377295
7	44	-0.009131099433211887
7	45	8.711416744446865E-4
7	46	-0.20588232953875973
7	47	-0.16449457026056535
7	48	-0.2900470236705388
7	49	-0.30418411353770125
7	50	-0.24728802516014525
8	1	-0.05383402901120604
8	2	-0.06204835607987036
8	3	-0.07010444265133486
8	4	-0.04857709951079414
8	5	-0.06279909241657577
8	6	-0.05310215932093598
8	7	-0.049691362259352445
8	8	-0.03363096962291934
8	9	-0.031659158434869994
8	10	-0.05388918938599829
8	11	-0.03707340543179072
8	12	-0.03330740090932238
8	13	-0.0405431961315017
8	14	-0.07661179899772463
8	15	-0.0718491348807504
8	16	-0.0611248456825777
8	17	-0.05235107916042381
8	18	-0.05315180966324817
8	19	-0.047741412696893804
8	20	-0.04847803598445921
8	21	-0.06943762921008556
8	22	-0.053516721082388585
8	23	-0.08168876288120686
8	24	-0.03363331635694777
8	25	-0.013183875233046649
8	26	-0.07460717218653201
8	27	-0.03880598523361556
8	28	-0.02448326243583665
8	29	-0.08479434490568849
8	30	-0.539187498043475
8	31	-0.1629067796166126
8	32	-0.117844506007458
8	33	-0.08218585708793569
8	34	-0.0940154995339526
8	35	-0.03579393788352547
8	36	7.590654580980072E-5
8	37	0.06202903392934189
8	38	0.036614524596565445
8	39	0.033704269809767595
8	40	-0.010943977225451818
8	41	0.08722075710110744
8	42	0.07499794350732714
8	43	0.1010038225731833
8	44	0.08088042768797266
8	45	0.0692726394211931
8	46	0.08195994622531089
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112	37	0.08872562529339945
112	38	0.07919379708087604
112	39	0.09789789607423671
112	40	0.09919702307416856
112	41	0.13666303852869532
112	42	0.13977309465055043
112	43	0.12413568545456855
112	44	0.16835754449700602
112	45	0.15745237688569347
112	46	0.14691796643614197
112	47	0.15226193767430374
112	48	0.19077710425525396
112	49	0.1645737449347635
112	50	0.2152589877058375
113	1	-0.1139947514490558
113	2	-0.12553179653465207
113	3	-0.11345949709023984
113	4	-0.12559789218490636
113	5	-0.13550167057048412
113	6	-0.09877500007452511
113	7	-0.1057105050933913
113	8	-0.09456845965975447
113	9	-0.10167469434964715
113	10	-0.1039447067946142
113	11	-0.07440323129110737
113	12	-0.0785745365489916
113	13	-0.10245493447970944
113	14	-0.08374133981104848
113	15	-0.07027105634684006
113	16	-0.09598482741424874
113	17	-0.0581107545855275
113	18	-0.08685537874097804
113	19	-0.08081435705026507
113	20	-0.07035256976448778
113	21	-0.11602082455961238
113	22	-0.10284336163203989
113	23	-0.1290692733462393
113	24	-0.12394424946987215
113	25	-0.06216927561755625
113	26	-0.011198650532058707
113	27	-0.07157609993958403
113	28	-0.10406245447518359
113	29	-0.1159587705282874
113	30	-0.10444885584081476
113	31	-0.0020660880240157553
113	32	-0.09212291828290375
113	33	-0.06310460932049722
113	34	-0.04214284384533329
113	35	-0.08453162278915016
113	36	0.0070935728570304946
113	37	-5.530903604835657E-4
113	38	0.04088486526049451
113	39	0.002214823672154864
113	40	0.06618948067276875
113	41	0.1433305065476418
113	42	0.13815191739448807
113	43	0.1640313737987853
113	44	0.1383716923061229
113	45	0.22448178350839498
113	46	0.171300320124935
113	47	0.1868307765914068
113	48	0.1657824326031303
113	49	0.17316652083658823
113	50	0.09561452221166888
114	1	-0.09442139852240672
114	2	-0.10269474471144946
114	3	-0.07501201087484333
114	4	-0.09592949035401688
114	5	-0.10358515410297997
114	6	-0.09179937886659673
114	7	-0.10671914057326859
114	8	-0.11607143243674756
114	9	-0.1111864685099988
114	10	-0.1339919309640365
114	11	-0.08207939468166359
114	12	-0.09485567998387268
114	13	-0.07919585538364515
114	14	-0.09612140451773854
114	15	-0.0744660110165043
114	16	-0.062194801682302625
114	17	-0.03351863713419334
114	18	-0.06412495979610355
114	19	-0.05061740889375699
114	20	-0.05641032498895271
114	21	-0.1320332312432413
114	22	-0.09677972715426364
114	23	-0.0858755914849354
114	24	-0.10129086875503646
114	25	-0.16137168033340998
114	26	-0.0947170299143707
114	27	-0.024208998122453806
114	28	-0.06530531934640749
114	29	-0.032713366544641076
114	30	-0.04581644274999519
114	31	-0.07778382762423774
114	32	-0.028720986144797678
114	33	-0.13334816286894124
114	34	-0.07109787372573351
114	35	-0.018200720235199697
114	36	0.02084652028887035
114	37	0.05850636374855611
114	38	0.059813743228289695
114	39	-0.0010701226641813832
114	40	0.029120785271636862
114	41	0.08501062053105102
114	42	0.10345092394225475
114	43	0.09666921699805187
114	44	0.0659849875576839
114	45	0.14962422916330098
114	46	0.055083686518997865
114	47	0.2159725400300445
114	48	0.12627343622031617
114	49	0.2054423737890403
114	50	0.0991215042938336
115	1	-0.13062317912966392
115	2	-0.12186889737581197
115	3	-0.14133137810332386
115	4	-0.1479553857862257
115	5	-0.12785482758074096
115	6	-0.10679356935312967
115	7	-0.12196704475249476
115	8	-0.10898628255302611
115	9	-0.11487283752960309
115	10	-0.11055414275097064
115	11	-0.09050648614961432
115	12	-0.10837081488697464
115	13	-0.12951910653908527
115	14	-0.11870750426553656
115	15	-0.14522672543151316
115	16	-0.09233029522373926
115	17	-0.09896282787931199
115	18	-0.07774212751175469
115	19	-0.0821038513511283
115	20	-0.11701083388164335
115	21	-0.08575588817337376
115	22	-0.1270180939052281
115	23	-0.12065772611050107
115	24	-0.07761080917735796
115	25	-0.06327199028905994
115	26	-0.13671966143434133
115	27	-0.13759083313572518
115	28	-0.19289216898689432
115	29	-0.15031267991876263
115	30	-0.18767758589675054
115	31	-0.16687334153862565
115	32	-0.2631628461889619
115	33	-0.25157109389778043
115	34	-0.12489640341890151
115	35	-0.12774174712273378
115	36	-0.09426459312076219
115	37	-0.07838286158644081
115	38	-0.07430663264895543
115	39	-0.04118423133483873
115	40	0.016467423597532616
115	41	0.10159122192774106
115	42	0.0419966148175277
115	43	0.09006330887800829
115	44	0.06127917390687543
115	45	0.08977087628103675
115	46	0.03993451821032323
115	47	0.05792882042440084
115	48	0.08163508274331122
115	49	0.05730992981119698
115	50	-0.05337971104949446
116	1	-0.1569109959078716
116	2	-0.15369036205875375
116	3	-0.15620803722669763
116	4	-0.1705591863222793
116	5	-0.16236932835914786
116	6	-0.14767701744082018
116	7	-0.15212810209757777
116	8	-0.15458236325022057
116	9	-0.15975214483054856
116	10	-0.14041330785280337
116	11	-0.14466075453037064
116	12	-0.1384529083917201
116	13	-0.1439090627237789
116	14	-0.13394624148607193
116	15	-0.14533313402123582
116	16	-0.15385211460198178
116	17	-0.15113698166620537
116	18	-0.15595621260653303
116	19	-0.13306554104891433
116	20	-0.1556855413783964
116	21	-0.1942075067716047
116	22	-0.20341633166342632
116	23	-0.23440907026123625
116	24	-0.20463273287774797
116	25	-0.12970882724156496
116	26	-0.16519495313511356
116	27	-0.22086173367659256
116	28	-0.1660999145050539
116	29	-0.21978577282966683
116	30	-0.19024174401702965
116	31	-0.19848848911433947
116	32	-0.20122935556992871
116	33	-0.23985034021039553
116	34	-0.22983268666155254
116	35	-0.17205113451098697
116	36	-0.07567149764192038
116	37	-0.08130171500580019
116	38	-0.10808108816759443
116	39	-0.1023783186765499
116	40	-0.04333500630600895
116	41	-0.06413309476219098
116	42	-0.1308791455304288
116	43	-0.028275080067054148
116	44	-0.056783242836026204
116	45	-0.19547572522441214
116	46	-0.16845009347054685
116	47	-0.18592580265165992
116	48	-0.13277910827856232
116	49	-0.17311811198186433
116	50	-0.2975130347530879
117	1	-0.15139985245532728
117	2	-0.17318992636997876
117	3	-0.14667823554080428
117	4	-0.14453724564666715
117	5	-0.14812334745634104
117	6	-0.16230285811468548
117	7	-0.1336225646273448
117	8	-0.1366215308104799
117	9	-0.13841027410530415
117	10	-0.14410850102684236
117	11	-0.12500606340108789
117	12	-0.15170941480417355
117	13	-0.14122793367144482
117	14	-0.16780454143394508
117	15	-0.13118354767540552
117	16	-0.14663059751379137
117	17	-0.18842907486557436
117	18	-0.16314134557933357
117	19	-0.16047618348939352
117	20	-0.15602171619506322
117	21	-0.141423302734502
117	22	-0.18311760566783875
117	23	-0.11890243779857457
117	24	-0.12519189815915865
117	25	-0.15858184961413002
117	26	-0.13061280275250908
117	27	-0.09997482049720219
117	28	-0.13851751822613068
117	29	-0.18467577925926548
117	30	-0.1894709691474432
117	31	-0.17046260155606774
117	32	-0.11649168210662708
117	33	-0.17157343561135718
117	34	-0.10730514474010278
117	35	-0.09611091866926103
117	36	-0.00971098701548101
117	37	-0.01994075429436748
117	38	-0.01361263180805139
117	39	0.022081525308038863
117	40	0.013126138807642462
117	41	0.07018560100269156
117	42	0.0894779658296514
117	43	0.13721422224251967
117	44	0.11252809405139885
117	45	0.1252616221561027
117	46	0.035708454815257085
117	47	0.038279267135258976
117	48	0.004636432074917707
117	49	0.07560775152046517
117	50	0.05296604678215289
118	1	-0.19020076879876768
118	2	-0.1767394682827188
118	3	-0.1776389919215262
118	4	-0.1795167070610617
118	5	-0.18085192428760166
118	6	-0.19442012987052948
118	7	-0.15769896299625685
118	8	-0.1875679482525996
118	9	-0.19441096095635402
118	10	-0.17595739468597316
118	11	-0.1590745259152868
118	12	-0.15844762126027234
118	13	-0.18122556482450136
118	14	-0.1783796737275125
118	15	-0.17423210084090357
118	16	-0.17377026226927939
118	17	-0.1659993629695009
118	18	-0.1713477025278678
118	19	-0.1700747020923643
118	20	-0.18203179513920986
118	21	-0.1867186221559507
118	22	-0.1631507886714445
118	23	-0.24126677701840293
118	24	-0.13813502084937568
118	25	-0.1674157118059938
118	26	-0.2293626194332532
118	27	-0.178792416251369
118	28	-0.23375473595355345
118	29	-0.24308133847377178
118	30	-0.17913853706126304
118	31	-0.3415668497373119
118	32	-0.22651626141293946
118	33	-0.240603870571654
118	34	-0.18201224953628525
118	35	-0.1799396885747626
118	36	-0.07174009339493637
118	37	-0.11241373119312215
118	38	-0.07111195484973365
118	39	-0.08152960667830911
118	40	-0.02286582381941571
118	41	-0.02950732586212723
118	42	-0.00989258856643005
118	43	0.014886513063501638
118	44	-0.034241810052598964
118	45	-0.013089416134512533
118	46	-0.02775345816436925
118	47	-0.06926141194644941
118	48	-0.09257289157279303
118	49	-0.06831993667921665
118	50	-0.11921428873485951
119	1	-0.18004555976560965
119	2	-0.19332010742719064
119	3	-0.20251316943001285
119	4	-0.18816851907568122
119	5	-0.20236895728815796
119	6	-0.17398825641239313
119	7	-0.1824035507422792
119	8	-0.18675935456954562
119	9	-0.19145812514311444
119	10	-0.19540715735118397
119	11	-0.1768735435979849
119	12	-0.16480353389813018
119	13	-0.1716556095769235
119	14	-0.1941593157482444
119	15	-0.20371563558570216
119	16	-0.1918133233710293
119	17	-0.21120927689663915
119	18	-0.19928753950602385
119	19	-0.19154985919139733
119	20	-0.215669320275218
119	21	-0.24638417472906582
119	22	-0.211991681470721
119	23	-0.28070791016080676
119	24	-0.17659807590769105
119	25	-0.22432850465895626
119	26	-0.28956858133206254
119	27	-0.281667084944317
119	28	-0.32823158480423587
119	29	-0.27367263805642494
119	30	-0.2623206771773354
119	31	-0.36536216568303814
119	32	-0.2616489785458995
119	33	-0.3276989156821273
119	34	-0.23614207027818424
119	35	-0.18597818672571265
119	36	-0.14471729124746702
119	37	-0.1607219165874625
119	38	-0.13190118447111843
119	39	-0.14630064503719353
119	40	-0.19535517242388778
119	41	-0.18474628956942496
119	42	-0.16457154835554633
119	43	-0.08734106258484076
119	44	-0.15117061921306174
119	45	-0.15331879029196926
119	46	-0.18572814464812382
119	47	-0.2546339693674966
119	48	-0.2556111273579731
119	49	-0.24904166923798243
119	50	-0.255346420168884
120	1	-0.21441307361504158
120	2	-0.21257731764474386
120	3	-0.2452042626703843
120	4	-0.22031952797620136
120	5	-0.21115657246356534
120	6	-0.2155342610889477
120	7	-0.2019488588076257
120	8	-0.2188223892667267
120	9	-0.23267352375818007
120	10	-0.2500531517221134
120	11	-0.2284373417311656
120	12	-0.2337769792787867
120	13	-0.24175773179111815
120	14	-0.22965687800165568
120	15	-0.261558540786325
120	16	-0.25748039927173494
120	17	-0.2457835314326644
120	18	-0.2479222128507672
120	19	-0.23073741708331852
120	20	-0.2591043857802191
120	21	-0.28384992486141414
120	22	-0.290935307967672
120	23	-0.3182968869978353
120	24	-0.20664271905977216
120	25	-0.2743084739720061
120	26	-0.1953313947342039
120	27	-0.19657485473695857
120	28	-0.23582732934675477
120	29	-0.2054237535541361
120	30	-0.22851476383878833
120	31	-0.1864989938329984
120	32	-0.14421627425700478
120	33	-0.17024983232934687
120	34	-0.1256988261405212
120	35	-0.09003282431267223
120	36	-0.07273975061922044
120	37	-0.01893518873437472
120	38	-0.07590369588988466
120	39	-0.01184350062989381
120	40	-0.033980848161832
120	41	0.022482388171859213
120	42	-0.09369457260178393
120	43	0.04057145023450204
120	44	0.05342110965388969
120	45	0.018757714902150724
120	46	-0.05734759650303545
120	47	-1.311742796872295E-4
120	48	-0.08570968160441339
120	49	-0.03986001129766237
120	50	-0.03779750721855635
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	22553.0
3	692.0
4	1193.0
5	1294.0
6	2136.0
7	3591.0
8	4354.0
9	4857.0
10	7050.0
11	9571.0
12	9914.0
13	13220.0
14	17746.0
15	20844.0
16	22104.0
17	24812.0
18	29207.0
19	36456.0
20	44192.0
21	37650.0
22	46801.0
23	59462.0
24	70847.0
25	79589.0
26	104202.0
27	127877.0
28	139825.0
29	150960.0
30	174901.0
31	210416.0
32	247928.0
33	313002.0
34	440236.0
35	643459.0
36	1003970.0
37	1807116.0
38	3695111.0
39	1.1195881E7
40	138219.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	19.46072452530755	29.25222198454748	28.16290567954965	23.12414781059532
2	22.524420315790813	28.80591452222828	27.345468228318975	21.32419693366193
3	22.466666676207204	27.818292462389177	27.453791483267736	22.261249378135886
4	22.979263999172627	27.26106024117766	27.36504483363586	22.39463092601385
5	23.50193226123508	28.130472140347823	27.39181369235058	20.975781906066516
6	23.468001168140372	27.642900899737132	26.329544382321927	22.55955354980057
7	23.10475165824883	27.695423257552548	27.531106968301494	21.668718115897125
8	22.65789950333496	27.889132315197905	27.277222591201745	22.17574559026539
9	22.325187194488784	27.62844589171971	27.745527119301876	22.300839794489626
10	22.30812397940317	28.017398468861472	27.314424536989158	22.360053014746207
11	22.410976019532868	28.079726080353655	27.171435620506536	22.337862279606938
12	22.295932261942735	27.936930308855658	27.42050685622149	22.346630572980118
13	22.29665095963985	27.953646835609153	27.300212195363606	22.44949000938739
14	22.402017479062312	28.06442556920487	27.341501932352053	22.192055019380767
15	22.319549897069653	27.977215316074044	27.277193539823706	22.4260412470326
16	22.34139389450725	27.82187776287443	27.369762148204124	22.466966194414198
17	22.699918986717172	27.705917824014612	27.353782186894964	22.24038100237325
18	22.36790052986569	27.652227563901512	27.337586270669934	22.642285635562867
19	22.45958173146115	27.84317932669825	27.336048531831757	22.361190410008838
20	22.66689818315212	27.565241615915102	27.413156274104704	22.35470392682807
21	22.4080831055138	27.852330008305014	27.31675891549155	22.42282797068963
22	22.681504710867554	27.628156538853982	27.20920392515848	22.481134825119984
23	22.45972995540411	27.70758155896653	27.431727346658302	22.400961138971056
24	22.421358537768516	27.80572399584546	27.248372770360163	22.524544696025867
25	22.51362388232206	27.61693360173715	27.259993300242325	22.609449215698465
26	22.64532805793013	27.679662866138173	27.355263954447896	22.319745121483805
27	22.608156552798064	27.56850103999784	27.299431289599713	22.523911117604385
28	22.656840756718715	27.6910135128069	27.20171249863367	22.45043323184072
29	22.60357448401687	27.756287915707293	27.243207078768684	22.396930521507148
30	22.556137374919928	27.665571273742458	27.244897978678146	22.533393372659475
31	22.600971181307447	27.724710410380162	27.177107914219594	22.4972104940928
32	22.668899999828042	27.69993242108485	27.142559221350275	22.48860835773683
33	22.56933857913985	27.788865413966846	27.02770074247743	22.614095264415873
34	22.60314757889054	27.827891268096305	27.049215951742294	22.51974520127086
35	22.66052720219304	27.805968141015423	27.03373817771962	22.49976647907192
36	22.592003069136098	27.78088541387435	27.055398126217174	22.571713390772377
37	22.64450837939167	27.80626291534257	27.00557493092324	22.543653774342523
38	22.725373313746434	27.771849226346717	27.02961145542222	22.47316600448463
39	22.6577427084557	27.726926305716244	26.993526692128217	22.621804293699842
40	22.66781084025853	27.776321193184867	27.000217588431646	22.555650378124955
41	22.66205802129945	27.84409114170448	26.946981893375167	22.546868943620904
42	22.63852277270771	27.77246127945292	26.928390890649474	22.6606250571899
43	22.695140058642515	27.78159724608561	26.92117809819071	22.60208459708117
44	22.662134991666864	27.807668206400376	26.945148825955435	22.585047975977325
45	22.631343077079446	27.766712317070503	26.902623127725484	22.69932147812457
46	22.693942877088652	27.83954696803223	26.819963826104665	22.646546328774456
47	22.714187651637154	27.881878027330558	26.806924938589184	22.597009382443105
48	22.686282832350248	27.750353991376908	26.781293175115167	22.782070001157678
49	22.75148891859522	27.787157339065132	26.71250708852147	22.748846653818173
50	22.64825195544819	27.871697900068114	26.77777176792811	22.702278376555586
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	172040.0
1	95313.0
2	18586.0
3	13695.0
4	8804.0
5	8225.0
6	7646.0
7	8151.5
8	8657.0
9	9965.5
10	11274.0
11	14419.5
12	17565.0
13	24266.0
14	30967.0
15	41872.5
16	52778.0
17	73229.5
18	93681.0
19	124064.0
20	154447.0
21	198724.0
22	243001.0
23	304965.0
24	366929.0
25	395582.0
26	424235.0
27	475709.5
28	527184.0
29	580200.0
30	633216.0
31	702643.5
32	772071.0
33	844697.5
34	917324.0
35	986580.5
36	1055837.0
37	1106217.5
38	1156598.0
39	1187769.0
40	1218940.0
41	1236431.5
42	1253923.0
43	1271413.5
44	1288904.0
45	1284627.5
46	1280351.0
47	1239279.5
48	1198208.0
49	1171254.0
50	1144300.0
51	1112275.5
52	1080251.0
53	1054016.0
54	1027781.0
55	992986.5
56	958192.0
57	915728.0
58	873264.0
59	838314.5
60	803365.0
61	729218.0
62	655071.0
63	564686.0
64	474301.0
65	407376.5
66	340452.0
67	294775.5
68	249099.0
69	207861.5
70	166624.0
71	135872.5
72	105121.0
73	86419.0
74	67717.0
75	52707.0
76	37697.0
77	30110.0
78	22523.0
79	18422.0
80	14321.0
81	12153.0
82	9985.0
83	8660.5
84	7336.0
85	6250.5
86	5165.0
87	4456.5
88	3748.0
89	2697.0
90	1646.0
91	1085.5
92	525.0
93	383.0
94	241.0
95	208.0
96	175.0
97	319.5
98	464.0
99	586.0
100	708.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4948281367601703
2	2.719045597822245E-4
3	2.719045597822245E-4
4	0.002466222059779124
5	0.0011162397717375532
6	1.9081021739103474E-5
7	4.2932298912982814E-5
8	7.155383152163803E-5
9	6.678357608686215E-5
10	6.678357608686215E-5
11	4.770255434775868E-5
12	7.155383152163803E-5
13	4.2932298912982814E-5
14	0.0021132231576057093
15	1.5741842934760366E-4
16	7.63240869564139E-5
17	4.2932298912982814E-5
18	4.770255434775868E-6
19	3.3391788043431074E-5
20	3.816204347820695E-5
21	3.816204347820695E-5
22	3.816204347820695E-5
23	0.006873938081512026
24	7.393895923902596E-4
25	0.020373760961927733
26	0.08457662885857614
27	0.08816863120096237
28	0.10561822558137249
29	0.10911005255962844
30	0.12331110298895619
31	0.1360810767878512
32	0.1322028591193784
33	0.16467398786389775
34	0.11918960229330984
35	0.05831637269013499
36	0.07931503711401837
37	0.0775261913259774
38	0.07909560536401866
39	0.10426824329333093
40	0.09554344610312586
41	0.09260019849986915
42	0.11556420816288017
43	0.12014842363569979
44	0.15429868229326024
45	0.2208628266301227
46	0.29037498882567664
47	0.2986418414941432
48	0.3241627080701941
49	0.34378753892886205
50	0.34158845117343034
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2.0963238E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.177201040676294
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.83939547795255	51.9808956549027
2	6.843433461564678	8.099504755270086
3	1.9599138142485377	3.479466414245532
4	0.8936981067880844	2.1154621014028105
5	0.5296648414844963	1.567204140435299
6	0.34819723637286987	1.2363202715187125
7	0.25058819547982064	1.0380375615630746
8	0.2009360182772054	0.9512664919922552
9	0.15146128745397863	0.806674955178938
>10	0.7834425288875154	8.12189643110709
>50	0.06817681847365574	2.9638701653092467
>100	0.12724466696143139	15.037252309085677
>500	0.003476026888984354	1.2954644775552784
>1k	3.311936340768336E-4	0.3135990603411671
>5k	2.419531932300846E-5	0.08606140308534833
>10k+	1.6130212882003383E-5	0.9070238070069802
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	160017	0.7633219639065301	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	30027	0.143236459940015	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4310766304327604E-5	4.770255434775868E-6	0.0	0.0	0.0
2	1.4310766304327604E-5	4.770255434775868E-6	0.0	0.0	0.0
3	2.385127717387934E-5	9.540510869551737E-6	0.0	4.770255434775868E-6	0.0
4	2.385127717387934E-5	1.4310766304327604E-5	0.0	4.770255434775868E-6	4.770255434775868E-6
5	2.385127717387934E-5	1.4310766304327604E-5	0.0	9.540510869551737E-6	4.770255434775868E-6
6	2.385127717387934E-5	1.9081021739103474E-5	0.0	9.540510869551737E-6	4.770255434775868E-6
7	2.8621532608655207E-5	2.8621532608655207E-5	0.0	1.4310766304327604E-5	4.770255434775868E-6
8	3.3391788043431074E-5	3.3391788043431074E-5	0.0	2.385127717387934E-5	4.770255434775868E-6
9	3.3391788043431074E-5	3.3391788043431074E-5	0.0	2.385127717387934E-5	4.770255434775868E-6
10	1.9081021739103472E-4	3.3391788043431074E-5	0.0	2.385127717387934E-5	4.770255434775868E-6
11	2.0035072826058646E-4	3.3391788043431074E-5	0.0	2.385127717387934E-5	4.770255434775868E-6
12	2.098912391301382E-4	4.2932298912982814E-5	0.0	2.385127717387934E-5	4.770255434775868E-6
13	2.098912391301382E-4	4.2932298912982814E-5	0.0	2.8621532608655207E-5	4.770255434775868E-6
14	2.242020054344658E-4	4.2932298912982814E-5	0.0	3.3391788043431074E-5	4.770255434775868E-6
15	2.3374251630401755E-4	4.770255434775868E-5	0.0	3.3391788043431074E-5	4.770255434775868E-6
16	2.480532826083451E-4	5.247280978253455E-5	0.0	3.3391788043431074E-5	4.770255434775868E-6
17	2.480532826083451E-4	5.247280978253455E-5	0.0	3.816204347820695E-5	4.770255434775868E-6
18	2.671343043474486E-4	5.7243065217310414E-5	0.0	4.770255434775868E-5	4.770255434775868E-6
19	3.1960711412998315E-4	5.7243065217310414E-5	0.0	4.770255434775868E-5	4.770255434775868E-6
20	3.5299890217341425E-4	5.7243065217310414E-5	0.0	4.770255434775868E-5	4.770255434775868E-6
21	4.674850326080351E-4	6.201332065208628E-5	0.0	5.247280978253455E-5	4.770255434775868E-6
22	4.913363097819144E-4	6.201332065208628E-5	0.0	5.247280978253455E-5	4.770255434775868E-6
23	5.199578423905697E-4	6.201332065208628E-5	0.0	5.7243065217310414E-5	4.770255434775868E-6
24	5.676603967383283E-4	7.63240869564139E-5	0.0	6.201332065208628E-5	4.770255434775868E-6
25	6.15362951086087E-4	7.63240869564139E-5	0.0	6.678357608686215E-5	4.770255434775868E-6
26	6.630655054338457E-4	7.63240869564139E-5	0.0	6.678357608686215E-5	4.770255434775868E-6
27	7.203085706511561E-4	8.109434239118976E-5	0.0	6.678357608686215E-5	4.770255434775868E-6
28	7.9663265760757E-4	8.586459782596563E-5	0.0	6.678357608686215E-5	9.540510869551737E-6
29	8.634162336944322E-4	9.06348532607415E-5	0.0	7.155383152163803E-5	9.540510869551737E-6
30	9.635915978247253E-4	9.06348532607415E-5	0.0	7.63240869564139E-5	9.540510869551737E-6
31	0.0011257802826071049	9.540510869551736E-5	0.0	7.63240869564139E-5	9.540510869551737E-6
32	0.0012975094782590362	9.540510869551736E-5	0.0	7.63240869564139E-5	9.540510869551737E-6
33	0.0019987370271710887	9.540510869551736E-5	0.0	7.63240869564139E-5	9.540510869551737E-6
34	0.0021847769891273476	9.540510869551736E-5	0.0	8.109434239118976E-5	9.540510869551737E-6
35	0.002289722608692417	9.540510869551736E-5	0.0	9.06348532607415E-5	9.540510869551737E-6
36	0.002461451804344348	9.540510869551736E-5	0.0	9.06348532607415E-5	9.540510869551737E-6
37	0.0026856538097788135	9.540510869551736E-5	0.0	1.0017536413029323E-4	9.540510869551737E-6
38	0.002871693771735073	1.0017536413029323E-4	0.0	1.0017536413029323E-4	9.540510869551737E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	12810	0.0	17.153404	1
AGCAGTG	14700	0.0	15.113053	2
ATCAACG	14955	0.0	14.282063	11
TCAACGC	15105	0.0	14.169298	12
GTGGTAT	15350	0.0	14.071678	6
GGTATCA	15265	0.0	13.9775305	8
CAACGCA	15265	0.0	13.93438	13
TGGTATC	15795	0.0	13.577849	7
AACGCAG	16800	0.0	12.674288	14
GTATCAA	16940	0.0	12.660373	9
CGCAGAG	17050	0.0	12.501097	16
AGTGGTA	17375	0.0	12.431789	5
GAGTACT	12555	0.0	12.278874	20
ACGCAGA	17505	0.0	12.226431	15
GTACTTT	12625	0.0	11.953301	22
CAGTGGT	18310	0.0	11.929312	4
AGAGTAC	18610	0.0	11.467943	19
AGTACTT	13675	0.0	11.17809	21
GCAGAGT	20195	0.0	10.7726145	17
CAGAGTA	20110	0.0	10.719877	18
>>END_MODULE