FastQCFastQC Report
Fri 27 May 2016
SRR522062_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522062_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15776788
Sequences flagged as poor quality0
Sequence length53
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT159150.022.975811
GCTCGTA16100.020.58074610
CTCGTAT16900.019.6065111
TCAACGC197850.019.38438412
CAACGCA200200.019.18031913
ATCAACG200800.019.12300711
TATGCCG17700.019.11864315
ACGCAGA205400.018.63753515
AACGCAG208700.018.45543914
GTGGTAT208900.018.3365256
GGTATCA209600.018.2640748
AGTGGTA210250.018.2187865
CGCAGAG210550.018.2151516
GTATCAA213000.017.9504729
TGGTATC214750.017.7823077
TATCAAC216600.017.68467310
GCAGTGG222650.017.330793
CGTATGC19850.016.9294713
TCGTATG19200.016.76822912
AGCAGTG233700.016.511342
Produced by FastQC (version 0.11.5)