##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522062_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15776788
Sequences flagged as poor quality	0
Sequence length	53
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.55743849762068	37.0	35.0	37.0	33.0	37.0
2	35.77321955520984	37.0	35.0	37.0	35.0	37.0
3	37.68303282011522	39.0	37.0	39.0	35.0	39.0
4	37.668239251234155	39.0	38.0	39.0	35.0	39.0
5	37.656539784904254	39.0	38.0	39.0	35.0	39.0
6	37.677506346665744	39.0	37.0	39.0	35.0	39.0
7	37.66861569034204	39.0	37.0	39.0	35.0	39.0
8	39.14014924964448	40.0	39.0	41.0	36.0	41.0
9	39.05689909758564	40.0	39.0	41.0	36.0	41.0
10	39.0164326857913	40.0	39.0	41.0	36.0	41.0
11	38.95457465740175	40.0	39.0	41.0	36.0	41.0
12	38.9195194230917	40.0	39.0	41.0	35.0	41.0
13	39.04126036300925	40.0	39.0	41.0	36.0	41.0
14	39.04906277500845	40.0	39.0	41.0	36.0	41.0
15	38.92387588652392	40.0	39.0	41.0	35.0	41.0
16	38.942172576572624	40.0	39.0	41.0	35.0	41.0
17	38.90773191602752	40.0	39.0	41.0	35.0	41.0
18	38.87793339176517	40.0	39.0	41.0	35.0	41.0
19	38.83939215003713	40.0	39.0	41.0	35.0	41.0
20	38.68049155506178	40.0	38.0	41.0	34.0	41.0
21	38.672738139093966	40.0	38.0	41.0	34.0	41.0
22	38.624612753876136	40.0	38.0	41.0	34.0	41.0
23	33.05864831295192	40.0	36.0	41.0	0.0	41.0
24	35.32599759849724	40.0	36.0	41.0	17.0	41.0
25	36.93500407053705	40.0	35.0	41.0	30.0	41.0
26	37.64183305245656	40.0	37.0	41.0	32.0	41.0
27	37.9401897268316	40.0	38.0	41.0	33.0	41.0
28	38.002353520881435	40.0	38.0	41.0	33.0	41.0
29	38.037069015568946	40.0	38.0	41.0	33.0	41.0
30	37.94317975243123	40.0	38.0	41.0	33.0	41.0
31	37.920814870555404	40.0	38.0	41.0	33.0	41.0
32	38.079922224980145	40.0	38.0	41.0	33.0	41.0
33	38.15597503116604	40.0	38.0	41.0	33.0	41.0
34	38.128786353724216	40.0	38.0	41.0	33.0	41.0
35	38.13234962655263	40.0	38.0	41.0	33.0	41.0
36	38.08605198979666	40.0	38.0	41.0	33.0	41.0
37	37.75069272655499	40.0	38.0	41.0	33.0	41.0
38	37.79733764566019	40.0	38.0	41.0	33.0	41.0
39	37.8216223099404	40.0	38.0	41.0	33.0	41.0
40	37.76567936388573	40.0	37.0	41.0	33.0	41.0
41	37.67571079740692	40.0	37.0	41.0	33.0	41.0
42	37.62657931386287	40.0	37.0	41.0	32.0	41.0
43	37.709582077162985	40.0	37.0	41.0	33.0	41.0
44	37.512313406252275	40.0	37.0	41.0	32.0	41.0
45	37.317564449747316	40.0	37.0	41.0	31.0	41.0
46	37.2910407365555	40.0	37.0	41.0	31.0	41.0
47	37.21304818192397	40.0	37.0	41.0	31.0	41.0
48	37.187073693327186	39.0	36.0	40.0	31.0	41.0
49	37.362451659995685	40.0	37.0	41.0	31.0	41.0
50	37.403401186604015	40.0	37.0	41.0	31.0	41.0
51	37.405326483438834	40.0	37.0	41.0	31.0	41.0
52	37.38440536819028	40.0	37.0	41.0	31.0	41.0
53	36.939997545761535	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	4.0
12	21.0
13	98.0
14	236.0
15	638.0
16	1393.0
17	2717.0
18	4760.0
19	7680.0
20	11915.0
21	17888.0
22	25649.0
23	35856.0
24	47642.0
25	63254.0
26	82023.0
27	104554.0
28	133089.0
29	165274.0
30	205393.0
31	253433.0
32	312082.0
33	386986.0
34	490386.0
35	642713.0
36	892273.0
37	1380359.0
38	2570578.0
39	3728410.0
40	4209480.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	13.541672740991388	28.20332630444169	28.479180933406724	29.7758200211602
2	25.067510573128065	25.03464583538804	30.195455500828178	19.702388090655713
3	16.46693230586606	25.809182452093545	32.42041409189247	25.303471150147928
4	17.490505672003707	28.741902344127336	31.826909254279133	21.940682729589824
5	23.279516717851568	28.02568558314912	29.33090056100139	19.363897137997924
6	21.739076420371497	27.227392546569046	29.86337903507355	21.170151997985904
7	22.77150456734286	27.99358145650433	29.060769530528013	20.1741444456248
8	21.08185772668049	26.354426515714096	29.64641472015723	22.91730103744818
9	20.846138009840786	25.689107313858816	31.154998089598468	22.30975658670193
10	21.77227709467859	27.01541023432653	28.739918416853925	22.472394254140955
11	21.552739378890053	27.659799954211213	28.445289370688126	22.342171296210612
12	21.636748874358965	27.329878553226422	28.350029169435505	22.683343402979112
13	21.900547817464496	27.584353671989508	27.58630590713395	22.928792603412052
14	22.271719693514296	27.922838286221506	27.400862583689406	22.404579436574796
15	21.908597618222416	27.625458363261266	27.557586499862964	22.908357518653354
16	22.36853280908636	27.391247191760453	27.291366278104263	22.948853721048923
17	23.147823245137097	26.942378892332204	27.1372411165061	22.772556746024602
18	22.391078589634343	27.32527051767445	27.040231509734426	23.243419382956784
19	22.755962747296852	27.485328445815462	27.121895787659696	22.636813019227993
20	22.988151960969493	27.17574071477667	27.154944339747733	22.6811629845061
21	22.71533977638541	27.509972245301135	27.23817420884403	22.536513769469426
22	22.9213703068077	26.94764612416672	27.389770338550534	22.74121323047505
23	19.252518319952074	37.816639229734214	23.668524924084675	19.26231752622904
24	22.25986049885439	27.363529255764863	27.467809036921835	22.90880120845891
25	22.37133439328715	27.159508006319154	27.317987666437553	23.15116993395614
26	22.519083098536914	27.13131468838904	27.81901487172167	22.53058734135237
27	22.730602705696494	26.80190669989354	27.86030971576724	22.607180878642723
28	23.1539968718601	26.779107382313818	27.320389929813345	22.746505816012743
29	23.31136096903882	26.815496284795103	27.302376123707816	22.57076662245826
30	23.020680762142458	26.835455987619277	27.346383813993064	22.797479436245197
31	22.910221015836683	26.97622608607024	27.283069278740385	22.830483619352684
32	23.00426423933693	27.035788273253086	27.229788471519043	22.73015901589094
33	22.78500541428331	27.03568685844039	27.266963338798746	22.912344388477553
34	22.812913503052712	27.096656176149413	27.16519357425605	22.92523674654182
35	22.901030298435902	27.104154533863294	27.26554353142097	22.72927163627983
36	22.814054419695566	27.003525685963453	27.2504644164579	22.93195547788308
37	23.15545470979264	27.040992120829664	27.08017626908595	22.723376900291743
38	22.9563520787628	27.106252552800985	27.20444110677028	22.732954261665935
39	22.775656236237694	27.04547972629156	27.26221585787931	22.916648179591434
40	22.903242409037887	27.096041348847432	27.165542187674703	22.835174054439978
41	22.883973594625218	27.063189287958995	27.269245172084457	22.78359194533133
42	22.812463474821364	26.93365088001436	27.29407278591815	22.959812859246128
43	22.84797767454313	27.04641781330902	27.199712641128222	22.905891871019627
44	23.03967068582021	26.984428009047218	27.22666996602857	22.749231339104007
45	22.874130019367694	26.909735999494956	27.265575223549938	22.950558757587412
46	22.83957926036656	26.976986697165483	27.213251518623437	22.970182523844525
47	22.98941330770243	27.01608844588645	27.16588446266756	22.82861378374356
48	22.861681351108984	26.91782383080764	27.252365944195994	22.968128873887384
49	22.888404154254975	26.994132138937278	27.152054017585836	22.965409689221914
50	23.04507736302218	27.0093633761194	27.14966443106163	22.795894829796786
51	22.917060177268024	26.889193161497765	27.19372282875323	23.000023832480984
52	22.97458139134531	26.944540295527837	27.082711639403406	22.998166673723446
53	23.067597789866987	26.980016464694838	27.076930995079607	22.875454750358564
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4093.0
1	2801.0
2	1509.0
3	1230.5
4	952.0
5	970.5
6	989.0
7	1186.5
8	1940.0
9	2496.0
10	3491.0
11	4486.0
12	6659.0
13	8832.0
14	13006.5
15	17181.0
16	25793.5
17	34406.0
18	48728.0
19	63050.0
20	80357.0
21	97664.0
22	134162.5
23	170661.0
24	185400.5
25	231457.0
26	262774.0
27	296937.5
28	331101.0
29	371439.0
30	411777.0
31	455815.0
32	499853.0
33	546049.5
34	592246.0
35	650278.5
36	708311.0
37	763278.0
38	818245.0
39	867671.5
40	917098.0
41	958165.5
42	1037415.0
43	1075597.0
44	1096347.0
45	1117097.0
46	1122989.5
47	1128882.0
48	1118199.5
49	1107517.0
50	1079798.5
51	1052080.0
52	1000759.0
53	949438.0
54	888622.0
55	827806.0
56	761008.5
57	694211.0
58	617659.0
59	468756.5
60	396406.0
61	338507.0
62	280608.0
63	235069.0
64	189530.0
65	153607.0
66	117684.0
67	93378.5
68	69073.0
69	53965.5
70	38858.0
71	30101.5
72	21345.0
73	15942.5
74	10540.0
75	8068.5
76	4068.0
77	2539.0
78	1867.5
79	1196.0
80	888.0
81	580.0
82	433.5
83	287.0
84	219.5
85	152.0
86	116.0
87	80.0
88	58.0
89	36.0
90	35.0
91	34.0
92	23.0
93	10.0
94	8.0
95	6.0
96	4.0
97	3.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.5776788E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.70870217643601
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.77318889078619	46.608006595060054
2	12.830599277159187	15.578580605245046
3	4.377622664115484	7.972793716698099
4	2.0330240074201726	4.936889959360629
5	1.1221113159829077	3.4060960845407515
6	0.6702793524093646	2.4415073748260707
7	0.4474321184868423	1.901411625777268
8	0.305439071900295	1.4834247719233629
9	0.23343928402999084	1.2754616373411454
>10	1.1665764356448574	12.331987497167015
>50	0.03460192430905359	1.3648018507176023
>100	0.005537781254431104	0.4754259899181808
>500	6.402485118791397E-5	0.02657944037659684
>1k	6.289111742632338E-5	0.06769730021845052
>5k	1.0480266258147862E-5	0.05053681032274449
>10k+	1.0480266258147862E-5	0.07879874050701126
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.394453674600939E-4	0.0	0.0	0.0	0.0
2	1.964911996028596E-4	0.0	0.0	0.0	0.0
3	1.964911996028596E-4	1.2676851587281264E-5	0.0	0.0	0.0
4	1.964911996028596E-4	1.9015277380921895E-5	0.0	6.338425793640632E-6	0.0
5	6.655347083322664E-4	1.9015277380921895E-5	0.0	6.338425793640632E-6	0.0
6	6.71873134125907E-4	1.9015277380921895E-5	0.0	1.2676851587281264E-5	0.0
7	6.972268373004695E-4	1.9015277380921895E-5	0.0	1.9015277380921895E-5	0.0
8	7.22580540475032E-4	1.9015277380921895E-5	0.0	2.535370317456253E-5	0.0
9	7.542726694432352E-4	1.9015277380921895E-5	0.0	2.535370317456253E-5	0.0
10	8.049800757923603E-4	1.9015277380921895E-5	0.0	2.535370317456253E-5	0.0
11	8.366722047605634E-4	1.9015277380921895E-5	0.0	3.169212896820316E-5	0.0
12	9.444254432524542E-4	3.169212896820316E-5	0.0	5.070740634912506E-5	0.0
13	0.0010268249785697825	3.803055476184379E-5	0.0	5.070740634912506E-5	6.338425793640632E-6
14	0.001083870810712548	3.803055476184379E-5	0.0	8.239953531732822E-5	6.338425793640632E-6
15	0.0011979624749980794	4.4368980555484424E-5	0.0	8.239953531732822E-5	6.338425793640632E-6
16	0.0012486698813472044	4.4368980555484424E-5	0.0	8.239953531732822E-5	6.338425793640632E-6
17	0.0012613467329344859	4.4368980555484424E-5	0.0	8.873796111096885E-5	6.338425793640632E-6
18	0.001293038861902689	4.4368980555484424E-5	0.0	8.873796111096885E-5	6.338425793640632E-6
19	0.0013183925650772515	9.507638690460947E-5	0.0	1.0141481269825012E-4	6.338425793640632E-6
20	0.0013564231198390953	9.507638690460947E-5	0.0	1.0141481269825012E-4	6.338425793640632E-6
21	0.0014071305261882203	9.507638690460947E-5	0.0	1.0141481269825012E-4	6.338425793640632E-6
22	0.0014578379325373453	9.507638690460947E-5	0.0	1.0141481269825012E-4	6.338425793640632E-6
23	0.0015022069130928297	9.507638690460947E-5	0.0	1.0775323849189074E-4	6.338425793640632E-6
24	0.0015592527452355956	9.507638690460947E-5	0.0	1.0775323849189074E-4	6.338425793640632E-6
25	0.0016099601515847206	9.507638690460947E-5	0.0	1.0775323849189074E-4	1.2676851587281264E-5
26	0.0016796828353147675	1.0141481269825012E-4	0.0	1.0775323849189074E-4	1.2676851587281264E-5
27	0.0017367286674575331	1.0775323849189074E-4	0.0	1.1409166428553138E-4	1.2676851587281264E-5
28	0.0018191282027748613	1.0775323849189074E-4	0.0	1.2043009007917201E-4	1.9015277380921895E-5
29	0.001914204589679471	1.1409166428553138E-4	0.0	1.2676851587281265E-4	1.9015277380921895E-5
30	0.0019902656992031587	1.2043009007917201E-4	0.0	1.3310694166645328E-4	1.9015277380921895E-5
31	0.002072665234520487	1.2676851587281265E-4	0.0	1.3310694166645328E-4	1.9015277380921895E-5
32	0.0021487263440441743	1.2676851587281265E-4	0.0	1.394453674600939E-4	1.9015277380921895E-5
33	0.0022945101372979088	1.3310694166645328E-4	0.0	1.394453674600939E-4	1.9015277380921895E-5
34	0.0023705712468215963	1.3310694166645328E-4	0.0	1.4578379325373453E-4	1.9015277380921895E-5
35	0.002497339762694409	1.3310694166645328E-4	0.0	1.4578379325373453E-4	1.9015277380921895E-5
36	0.002636785130154503	1.394453674600939E-4	0.0	1.4578379325373453E-4	1.9015277380921895E-5
37	0.002757215220233675	1.394453674600939E-4	0.0	1.4578379325373453E-4	1.9015277380921895E-5
38	0.002979060123011097	1.4578379325373453E-4	0.0	1.5846064484101581E-4	1.9015277380921895E-5
39	0.003226258728963082	1.5212221904737516E-4	0.0	1.5846064484101581E-4	1.9015277380921895E-5
40	0.0034671189091214256	1.5212221904737516E-4	0.0	1.5846064484101581E-4	1.9015277380921895E-5
41	0.003758686495628895	1.5212221904737516E-4	0.0	1.5846064484101581E-4	1.9015277380921895E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	15915	0.0	22.97581	1
GCTCGTA	1610	0.0	20.580746	10
CTCGTAT	1690	0.0	19.60651	11
TCAACGC	19785	0.0	19.384384	12
CAACGCA	20020	0.0	19.180319	13
ATCAACG	20080	0.0	19.123007	11
TATGCCG	1770	0.0	19.118643	15
ACGCAGA	20540	0.0	18.637535	15
AACGCAG	20870	0.0	18.455439	14
GTGGTAT	20890	0.0	18.336525	6
GGTATCA	20960	0.0	18.264074	8
AGTGGTA	21025	0.0	18.218786	5
CGCAGAG	21055	0.0	18.21515	16
GTATCAA	21300	0.0	17.950472	9
TGGTATC	21475	0.0	17.782307	7
TATCAAC	21660	0.0	17.684673	10
GCAGTGG	22265	0.0	17.33079	3
CGTATGC	1985	0.0	16.92947	13
TCGTATG	1920	0.0	16.768229	12
AGCAGTG	23370	0.0	16.51134	2
>>END_MODULE