##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522061_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20397597
Sequences flagged as poor quality	0
Sequence length	53
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.64934565576524	37.0	35.0	37.0	35.0	37.0
2	35.78309312611677	37.0	35.0	37.0	35.0	37.0
3	37.68053104490691	39.0	37.0	39.0	35.0	39.0
4	37.57319977446363	39.0	37.0	39.0	35.0	39.0
5	37.54044743603867	39.0	37.0	39.0	35.0	39.0
6	37.638122569045755	39.0	37.0	39.0	35.0	39.0
7	37.48894837955667	39.0	37.0	39.0	35.0	39.0
8	39.02686404677963	40.0	39.0	41.0	36.0	41.0
9	39.010459418332466	40.0	39.0	41.0	36.0	41.0
10	38.609842620187074	40.0	38.0	41.0	34.0	41.0
11	38.72245539511346	40.0	38.0	41.0	35.0	41.0
12	38.786877983715435	40.0	38.0	41.0	35.0	41.0
13	38.98485703977777	40.0	39.0	41.0	35.0	41.0
14	39.02226585808123	40.0	39.0	41.0	36.0	41.0
15	38.889589445266516	40.0	39.0	41.0	35.0	41.0
16	38.8867045466189	40.0	39.0	41.0	35.0	41.0
17	38.87676822912032	40.0	39.0	41.0	35.0	41.0
18	38.85510798159215	40.0	39.0	41.0	35.0	41.0
19	38.804791956621166	40.0	39.0	41.0	35.0	41.0
20	38.680266258814704	40.0	38.0	41.0	34.0	41.0
21	38.530206621887864	40.0	38.0	41.0	34.0	41.0
22	38.60366478463125	40.0	38.0	41.0	34.0	41.0
23	32.74818568089172	40.0	34.0	41.0	0.0	41.0
24	35.1548082354995	40.0	35.0	41.0	17.0	41.0
25	36.813198878279636	40.0	35.0	41.0	30.0	41.0
26	37.54144206300379	40.0	37.0	41.0	32.0	41.0
27	37.90024908326211	40.0	38.0	41.0	33.0	41.0
28	37.992456317280904	40.0	38.0	41.0	33.0	41.0
29	38.07933704151523	40.0	38.0	41.0	33.0	41.0
30	38.014011797566155	40.0	38.0	41.0	33.0	41.0
31	37.91050107520018	40.0	38.0	41.0	33.0	41.0
32	37.96253191000881	40.0	38.0	41.0	33.0	41.0
33	37.98439909367755	40.0	38.0	41.0	33.0	41.0
34	37.96812006826098	40.0	38.0	41.0	33.0	41.0
35	37.94141957015819	40.0	38.0	41.0	33.0	41.0
36	37.87369335711457	40.0	38.0	41.0	33.0	41.0
37	37.48527132877466	40.0	37.0	41.0	32.0	41.0
38	37.48592875915727	40.0	37.0	41.0	32.0	41.0
39	37.455510617255555	40.0	37.0	41.0	32.0	41.0
40	37.42054488085043	40.0	37.0	41.0	32.0	41.0
41	37.30606100316621	40.0	36.0	41.0	32.0	41.0
42	37.27084406069989	40.0	36.0	41.0	31.0	41.0
43	37.34082416668983	40.0	36.0	41.0	32.0	41.0
44	37.14322162556697	40.0	36.0	41.0	31.0	41.0
45	36.958795146310614	39.0	36.0	40.0	31.0	41.0
46	36.912538128878616	39.0	35.0	40.0	31.0	41.0
47	36.83903795138222	39.0	35.0	40.0	31.0	41.0
48	36.76853900976669	39.0	35.0	40.0	31.0	41.0
49	36.88546155706479	39.0	35.0	41.0	31.0	41.0
50	36.891483197751185	39.0	35.0	41.0	31.0	41.0
51	36.83176572220738	39.0	35.0	41.0	31.0	41.0
52	36.73432056727074	39.0	35.0	41.0	30.0	41.0
53	36.239125716622404	39.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	6.0
12	33.0
13	122.0
14	330.0
15	836.0
16	2060.0
17	4093.0
18	7096.0
19	11939.0
20	18333.0
21	26574.0
22	38182.0
23	52137.0
24	70023.0
25	91289.0
26	117755.0
27	150618.0
28	187215.0
29	232937.0
30	286755.0
31	350186.0
32	429556.0
33	535039.0
34	681587.0
35	906258.0
36	1291101.0
37	1979144.0
38	3374898.0
39	4666744.0
40	4884750.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	12.904667152704311	35.728438992102845	30.472927766932546	20.8939660882603
2	24.711768744131966	38.1178675115505	18.550930288504084	18.619433455813446
3	32.85334051849343	24.95885667316596	20.959968961049675	21.227833847290935
4	21.00029233835731	24.006342511816463	22.213405824225273	32.77995932560095
5	21.438794971780254	34.045549581158994	23.916915311151605	20.598740135909146
6	31.634402817155372	24.0575397190169	22.60881514621551	21.699242317612217
7	21.841528685952564	23.61087435936694	33.8602728546897	20.687324099990796
8	30.87462704552894	24.8195314379434	23.646339321244554	20.659502195283103
9	30.530262952052635	24.295342240559023	24.284051694912886	20.890343112475453
10	20.6108346978323	24.700571346713048	33.72428624803206	20.964307707422595
11	20.45940019307176	34.31288989580488	24.305412054174813	20.92229785694854
12	20.32910053081253	24.561888344004444	33.952651383395796	21.15635974178723
13	20.40309944352759	24.723441687763515	24.137103012673503	30.736355856035395
14	20.83373350301999	34.50031883657668	23.88866198307575	20.77728567732758
15	20.528937795957045	34.18771338604248	24.03572832623372	21.24762049176675
16	20.688554637097695	24.380798385221553	24.156281742403284	30.774365235277468
17	30.80656510666428	24.086322521226396	23.98544299115234	21.12166938095698
18	20.857040169976884	24.405737597423855	23.93504980022892	30.802172432370345
19	21.003282886704746	34.14070294652846	24.012804057262237	20.843210109504565
20	30.85884577482338	24.31195694277125	24.062638358822365	20.76655892358301
21	20.981103803550976	34.00003931835696	24.098333739998886	20.920523138093177
22	30.56515921949041	24.117845842331327	24.32342888233354	20.993566055844713
23	18.00211564136697	35.083127684109066	28.985590802681315	17.92916587184265
24	20.729525149457555	33.84135886202674	24.29156728608767	21.137548702428035
25	20.806377339448368	24.31813904353537	24.213783613824706	30.661700003191548
26	21.01300952264132	30.758132931050653	27.407110749369153	20.821746796938875
27	21.28674274719713	24.09598052162713	33.730345785339324	20.88693094583641
28	27.862929147977578	24.002013570520095	27.1444327486223	20.990624532880027
29	28.060633808972696	24.133254520128034	26.97108389777482	20.83502777312445
30	27.821649775706426	24.138005079716006	26.977864108208433	21.06248103636914
31	25.071188532649213	25.172911299306485	27.74228748611908	22.013612681925228
32	23.88365158895923	26.25445536550212	28.158223735864574	21.703669309674076
33	23.655796317576037	25.83766117155859	28.260314192892427	22.246228317972946
34	23.107579780108413	25.680441671634163	28.580018518848078	22.631960029409345
35	22.807632683398932	25.743110818396893	28.86325286257984	22.586003635624333
36	22.852069290318855	25.897957489796468	28.743782907368942	22.506190312515734
37	23.572894395354513	25.747341708927774	28.61344402480351	22.066319870914207
38	23.260769393571213	25.770207147439965	28.794852648574242	22.17417081041458
39	23.157752356809482	25.676191170950187	28.774825779722974	22.391230692517357
40	22.81877125035856	26.062839657043916	28.658473838854647	22.459915253742878
41	23.062755872664805	26.149492021045422	28.508093379823123	22.279658726466653
42	23.04479787496537	26.143873712182863	28.510387767735583	22.30094064511619
43	22.882195388015557	25.89586900849154	29.01175074691396	22.210184856578937
44	23.078218478382528	25.831949714468816	28.747557861840296	22.34227394530836
45	23.18375051727907	25.598887947438122	28.641962089946183	22.575399445336625
46	22.922955091229618	25.624680201300183	28.62893604575088	22.82342866171932
47	22.96873499363675	25.71252878464066	28.716564014869007	22.60217220685358
48	22.774893532801926	25.70055678617437	28.65702759006367	22.86752209096003
49	22.53253655320281	26.230030919818642	28.578180066995145	22.6592524599834
50	23.17200893811168	25.94410018003591	28.366351193231242	22.517539688621163
51	23.022260906517566	25.791288062020246	28.507524685383284	22.678926346078903
52	23.19968866920942	25.78162515908124	28.348653030060355	22.67003314164899
53	23.524560270506374	25.921499478590544	27.893927897487142	22.660012353415944
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3393.0
1	2147.0
2	901.0
3	748.5
4	596.0
5	609.5
6	623.0
7	745.5
8	1252.5
9	1637.0
10	2455.5
11	3274.0
12	5004.5
13	6735.0
14	10058.5
15	13382.0
16	20085.5
17	26789.0
18	39315.0
19	51841.0
20	70589.5
21	89338.0
22	343365.0
23	597392.0
24	428893.0
25	268574.5
26	276755.0
27	301964.0
28	327173.0
29	364968.0
30	402763.0
31	446637.5
32	490512.0
33	543456.0
34	596400.0
35	663441.5
36	730483.0
37	800259.5
38	870036.0
39	940494.5
40	1010953.0
41	1079453.5
42	1218565.5
43	1289177.0
44	1345807.5
45	1402438.0
46	1444662.5
47	1486887.0
48	1512176.0
49	1537465.0
50	1510374.0
51	1483283.0
52	1425160.0
53	1367037.0
54	1292804.5
55	1218572.0
56	1169684.5
57	1120797.0
58	973529.0
59	712312.5
60	598364.0
61	513363.5
62	428363.0
63	360774.5
64	293186.0
65	240395.0
66	187604.0
67	149874.5
68	112145.0
69	88330.5
70	64516.0
71	49841.5
72	35167.0
73	26562.0
74	17957.0
75	13784.0
76	6946.5
77	4282.0
78	3183.5
79	2085.0
80	1615.0
81	1145.0
82	867.5
83	590.0
84	411.5
85	233.0
86	166.0
87	99.0
88	84.0
89	69.0
90	55.0
91	41.0
92	30.0
93	12.0
94	5.0
95	5.0
96	5.0
97	3.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.0397597E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.21729850198726
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.37624575112997	42.37448907350423
2	13.415103095138301	15.083217102686458
3	4.628465985189916	7.80599561687148
4	2.191477133318478	4.927956966561768
5	1.199973023991811	3.3729620842039982
6	0.7459969745736277	2.5162760760711014
7	0.4834498933393184	1.9024772875227258
8	0.3517396770950876	1.581908353779772
9	0.24436075006365193	1.2363571105648041
>10	1.3004366192750845	12.891620189146133
>50	0.05005907368881528	1.8477621572735383
>100	0.011790438743726893	1.1212741934632338
>500	4.361788554980909E-4	0.16948278571745065
>1k	3.5135442270016015E-4	0.35808899937861455
>5k	4.386583618590169E-5	0.16397052250853314
>10k+	7.01853378974376E-5	2.646161480746116
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	354903	1.739925541229195	No Hit
AAGCAGTGGTATCAACGCAGAGAACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59798	0.29316198373759417	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	38676	0.18961057030394315	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	26477	0.12980450589351286	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7158883960694E-4	0.0	0.0	9.805076548967999E-6	0.0
2	2.3532183717523198E-4	4.9025382744839995E-6	0.0	9.805076548967999E-6	0.0
3	2.4512691372419995E-4	4.9025382744839995E-6	0.0	9.805076548967999E-6	0.0
4	2.4512691372419995E-4	4.9025382744839995E-6	0.0	1.4707614823451998E-5	0.0
5	2.5002945199868395E-4	4.9025382744839995E-6	0.0	1.4707614823451998E-5	4.9025382744839995E-6
6	2.5983452854765195E-4	9.805076548967999E-6	0.0	1.4707614823451998E-5	4.9025382744839995E-6
7	2.5983452854765195E-4	9.805076548967999E-6	0.0	1.4707614823451998E-5	9.805076548967999E-6
8	2.5983452854765195E-4	9.805076548967999E-6	0.0	1.9610153097935998E-5	9.805076548967999E-6
9	2.6963960509661996E-4	9.805076548967999E-6	0.0	1.9610153097935998E-5	9.805076548967999E-6
10	2.6963960509661996E-4	9.805076548967999E-6	0.0	1.9610153097935998E-5	1.4707614823451998E-5
11	2.7944468164558796E-4	9.805076548967999E-6	0.0	1.9610153097935998E-5	1.4707614823451998E-5
12	2.8434721992007196E-4	9.805076548967999E-6	0.0	2.4512691372419996E-5	1.4707614823451998E-5
13	2.9905483474352396E-4	9.805076548967999E-6	0.0	2.4512691372419996E-5	1.4707614823451998E-5
14	3.1376244956697597E-4	1.4707614823451998E-5	0.0	3.4317767921387995E-5	1.4707614823451998E-5
15	3.578852940373319E-4	2.4512691372419996E-5	0.0	3.9220306195871996E-5	1.4707614823451998E-5
16	3.7259290886078393E-4	2.9415229646903997E-5	0.0	4.412284447035599E-5	1.4707614823451998E-5
17	4.1181321505665594E-4	2.9415229646903997E-5	0.0	4.412284447035599E-5	1.4707614823451998E-5
18	4.6083859780149595E-4	2.9415229646903997E-5	0.0	4.412284447035599E-5	1.4707614823451998E-5
19	4.853512891739159E-4	5.392792101932399E-5	0.0	4.902538274483999E-5	1.4707614823451998E-5
20	5.196690570953039E-4	5.392792101932399E-5	0.0	5.392792101932399E-5	1.4707614823451998E-5
21	5.490842867422079E-4	5.392792101932399E-5	0.0	5.392792101932399E-5	1.4707614823451998E-5
22	6.079147460360159E-4	5.392792101932399E-5	0.0	5.392792101932399E-5	1.4707614823451998E-5
23	6.667452053298239E-4	5.392792101932399E-5	0.0	5.392792101932399E-5	1.4707614823451998E-5
24	6.667452053298239E-4	5.392792101932399E-5	0.0	5.8830459293807994E-5	1.4707614823451998E-5
25	7.157705880746638E-4	5.392792101932399E-5	0.0	7.353807411725999E-5	1.4707614823451998E-5
26	7.647959708195039E-4	5.392792101932399E-5	0.0	7.844061239174399E-5	1.4707614823451998E-5
27	8.628467363091839E-4	5.392792101932399E-5	0.0	7.844061239174399E-5	1.4707614823451998E-5
28	9.658000400733479E-4	5.392792101932399E-5	0.0	8.824568894071198E-5	1.9610153097935998E-5
29	0.0010491431907395758	5.8830459293807994E-5	0.0	9.314822721519598E-5	1.9610153097935998E-5
30	0.0011815117241506439	5.8830459293807994E-5	0.0	9.805076548967998E-5	1.9610153097935998E-5
31	0.00137271071685552	5.8830459293807994E-5	0.0	9.805076548967998E-5	1.9610153097935998E-5
32	0.0016031300157562677	5.8830459293807994E-5	0.0	9.805076548967998E-5	2.4512691372419996E-5
33	0.0017256934726183678	5.8830459293807994E-5	0.0	9.805076548967998E-5	2.4512691372419996E-5
34	0.0018678670825784038	5.8830459293807994E-5	0.0	9.805076548967998E-5	2.4512691372419996E-5
35	0.002005138154263956	5.8830459293807994E-5	0.0	1.0295330376416398E-4	2.4512691372419996E-5
36	0.0022208498383412515	5.8830459293807994E-5	0.0	1.0785584203864799E-4	2.9415229646903997E-5
37	0.0024120488310461277	5.8830459293807994E-5	0.0	1.0785584203864799E-4	3.4317767921387995E-5
38	0.0026326630533979076	5.8830459293807994E-5	0.0	1.0785584203864799E-4	3.4317767921387995E-5
39	0.0028924975819455594	6.373299756829199E-5	0.0	1.0785584203864799E-4	3.4317767921387995E-5
40	0.0030935016511994037	6.373299756829199E-5	0.0	1.0785584203864799E-4	3.4317767921387995E-5
41	0.0033827514093939595	6.373299756829199E-5	0.0	1.1275838031313199E-4	3.4317767921387995E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	212885	0.0	44.444508	1
AGTGGTA	216730	0.0	43.490124	5
ATCAACG	218750	0.0	43.435516	11
GTGGTAT	216975	0.0	43.42369	6
TCAACGC	219045	0.0	43.41564	12
CAACGCA	219670	0.0	43.348816	13
GTATCAA	218225	0.0	43.26326	9
GGTATCA	218310	0.0	43.25933	8
ACGCAGA	219880	0.0	43.21764	15
AGCAGTG	219615	0.0	43.18632	2
AACGCAG	220540	0.0	43.170353	14
TATCAAC	218850	0.0	43.133263	10
TGGTATC	218460	0.0	43.11668	7
GCAGTGG	220440	0.0	43.100384	3
CGCAGAG	220765	0.0	43.038002	16
CAGTGGT	221615	0.0	42.585564	4
AGAGTAC	188885	0.0	42.32824	19
CAGAGTA	192235	0.0	41.75564	18
ACATGGG	151500	0.0	41.626797	24
GAGTACT	60000	0.0	41.434414	20
>>END_MODULE