##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522060_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20028846
Sequences flagged as poor quality	0
Sequence length	53
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.11486033693604	37.0	35.0	37.0	35.0	37.0
2	36.119989788727715	37.0	35.0	37.0	35.0	37.0
3	37.8683407421476	39.0	38.0	39.0	35.0	39.0
4	37.82421009178462	39.0	38.0	39.0	35.0	39.0
5	37.72458423216195	39.0	38.0	39.0	35.0	39.0
6	37.68904718724184	39.0	37.0	39.0	35.0	39.0
7	37.55767576424523	39.0	37.0	39.0	35.0	39.0
8	39.063039777728584	40.0	39.0	41.0	36.0	41.0
9	39.09330422731295	40.0	39.0	41.0	36.0	41.0
10	38.67942236911702	40.0	38.0	41.0	34.0	41.0
11	38.90565347599158	40.0	38.0	41.0	36.0	41.0
12	38.99635575609299	40.0	39.0	41.0	36.0	41.0
13	39.1413469353152	40.0	39.0	41.0	36.0	41.0
14	39.17526366721278	40.0	39.0	41.0	36.0	41.0
15	39.17128899987548	40.0	39.0	41.0	36.0	41.0
16	39.10124182891016	40.0	39.0	41.0	36.0	41.0
17	39.036604854817895	40.0	39.0	41.0	36.0	41.0
18	38.96529974817321	40.0	39.0	41.0	35.0	41.0
19	38.94224904420354	40.0	39.0	41.0	35.0	41.0
20	38.915991565365275	40.0	39.0	41.0	35.0	41.0
21	38.74898628707815	40.0	38.0	41.0	35.0	41.0
22	38.85258521634247	40.0	39.0	41.0	35.0	41.0
23	37.05699744258855	40.0	37.0	41.0	32.0	41.0
24	37.8302042464154	40.0	38.0	41.0	33.0	41.0
25	38.31398139463452	40.0	38.0	41.0	33.0	41.0
26	38.36827973014521	40.0	38.0	41.0	34.0	41.0
27	38.531272246039535	40.0	38.0	41.0	34.0	41.0
28	38.57816730928981	40.0	38.0	41.0	34.0	41.0
29	38.41017335696725	40.0	38.0	41.0	34.0	41.0
30	38.444103819061766	40.0	38.0	41.0	34.0	41.0
31	38.19008603890609	40.0	38.0	41.0	33.0	41.0
32	38.30899219056355	40.0	38.0	41.0	34.0	41.0
33	37.992115521782935	40.0	38.0	41.0	33.0	41.0
34	38.19551226266356	40.0	38.0	41.0	33.0	41.0
35	38.26852056279228	40.0	38.0	41.0	33.0	41.0
36	38.25311528182902	40.0	38.0	41.0	33.0	41.0
37	38.00201833894973	40.0	37.0	41.0	33.0	41.0
38	37.93378535138769	40.0	37.0	41.0	33.0	41.0
39	37.98148600273825	40.0	37.0	41.0	33.0	41.0
40	37.95658356951768	40.0	37.0	41.0	33.0	41.0
41	37.865751426717246	40.0	37.0	41.0	33.0	41.0
42	37.80192438446029	40.0	37.0	41.0	33.0	41.0
43	37.0322980165707	40.0	36.0	41.0	31.0	41.0
44	37.14783018452486	40.0	35.0	41.0	31.0	41.0
45	37.34225915961409	40.0	36.0	41.0	32.0	41.0
46	37.36973568022841	40.0	36.0	41.0	32.0	41.0
47	37.394463914695834	40.0	36.0	41.0	32.0	41.0
48	37.381474299617665	40.0	36.0	41.0	32.0	41.0
49	37.18169893562515	40.0	35.0	41.0	31.0	41.0
50	37.05690068214614	39.0	35.0	41.0	31.0	41.0
51	36.83667830887511	39.0	35.0	41.0	31.0	41.0
52	36.694538766736734	39.0	35.0	41.0	31.0	41.0
53	36.1071957915099	39.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	12.0
13	18.0
14	99.0
15	232.0
16	646.0
17	1493.0
18	2854.0
19	5461.0
20	9483.0
21	15224.0
22	23269.0
23	33845.0
24	48477.0
25	66965.0
26	88793.0
27	115723.0
28	148139.0
29	186254.0
30	232619.0
31	289881.0
32	362455.0
33	457888.0
34	594592.0
35	809011.0
36	1153234.0
37	1831844.0
38	2637266.0
39	4661205.0
40	6251861.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.701707627089448	39.81131014737444	28.712003677096526	19.774978548439588
2	25.5099619818336	45.46382752156565	12.076087658769756	16.950122837830996
3	36.93708564137943	25.65642074436041	17.493678866970168	19.912814747289982
4	20.811044230905765	23.287582320019837	19.532448349745163	36.36892509932924
5	19.491931786783923	36.564932398002355	23.097097056914812	20.846038758298903
6	33.854207077132656	23.1155803983914	21.404588162493233	21.625624361982712
7	21.14059891418607	22.42847141567717	35.615696480965504	20.81523318917126
8	33.29421475406022	24.419349971536054	22.461059414007178	19.825375860396548
9	33.22872421107037	23.925502248107556	22.86027862014616	19.985494920675908
10	19.825380853195437	24.25531655692994	35.82121006871789	20.098092521156737
11	19.750119402785362	37.14701785614608	23.106733158765113	19.996129582303443
12	19.674628283626525	23.899190197977457	36.10852567342123	20.317655844974794
13	19.568556271289918	24.16034353651728	23.14851789264344	33.12258229954936
14	20.11793889672925	37.22159529310875	22.837796046761756	19.822669763400246
15	19.787889926359213	36.89048784937485	22.8594348371344	20.46218738713154
16	20.10756885344268	23.6319855871876	23.089088607501402	33.17135695186832
17	33.26166170532242	23.500110790207284	23.018430517664374	20.219796986805928
18	20.057690792569876	23.82940584794551	23.011205937676092	33.101697421808524
19	20.31035138020433	36.74817310992356	23.01058183781532	19.930893672056794
20	33.37444903216091	23.79455111892118	23.113024085361683	19.717975763556222
21	20.25392276719288	36.6449719569465	23.07955735442771	20.021547921432916
22	32.98505066143102	23.414359469337377	23.495896867947362	20.10469300128425
23	19.61476462498139	26.117435822313478	34.95209858820623	19.3157009644989
24	20.015591512361723	36.48679010263497	23.137893216613676	20.35972516838963
25	19.993822909218036	23.696931915098855	23.27525509956989	33.03399007611322
26	20.504032034596502	31.92068080207916	27.721012982974656	19.85427418034968
27	20.505779514206658	23.435329224659274	36.08051107887095	19.978380182263123
28	28.86790382231707	23.448844731244126	27.664289794828917	20.01896165160988
29	28.91285399068923	23.533048284459323	27.48529795475985	20.068799770091598
30	28.752685002421007	23.51896359880145	27.552825559695254	20.175525839082294
31	25.30702967110536	24.729971961440015	28.49083267203712	21.4721656954175
32	23.968060865813236	26.129788006757853	28.989059080088786	20.913092047340122
33	23.70304809373441	25.564832841592573	29.11098822168786	21.621130842985163
34	22.852689565839192	25.34804551395522	29.505209636141792	22.294055284063795
35	22.374034929421295	25.474013829853202	29.964487220082475	22.187464020643027
36	22.586753125966418	25.64872184847794	29.782819239810422	21.98170578574522
37	23.23739470561609	25.535844651259488	29.6512340251655	21.575526617958918
38	23.001350152674796	25.53210504489375	29.865465039773138	21.60107976265832
39	22.94373824632732	25.470608741012835	29.81818323432114	21.767469778338704
40	22.404116542710447	26.026561889786358	29.605290289815002	21.96403127768819
41	22.837850967549503	26.190695160370197	29.353044104488095	21.6184097675922
42	22.675804686900083	26.222604138051686	29.478163644575428	21.6234275304728
43	22.513588651088533	25.622324920766776	30.101314873557865	21.76277155458682
44	22.926178572644677	25.606977056990704	29.730924088187606	21.735920282177016
45	23.00273815076515	25.328403843137043	29.64494309856893	22.023914907528873
46	22.582768872455258	25.364506771882912	29.562112565047432	22.4906117906144
47	22.50601457517822	25.49377532784465	29.83269230788434	22.16751778909279
48	22.219897242207566	25.470079504330904	29.737374784348535	22.572648469113
49	22.067387207430723	26.201299865204415	29.45900128245032	22.272311644914538
50	22.959375692438798	25.871141053258885	29.17460147229651	21.994881782005812
51	22.67087180160055	25.714611815378678	29.520262924783587	22.094253458237183
52	22.99505423327934	25.538715510619035	29.30508827118647	22.161141984915155
53	23.45854573947995	25.806399430101962	28.64590900544145	22.089145824976637
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4355.0
1	2719.0
2	1083.0
3	828.5
4	574.0
5	570.0
6	566.0
7	675.5
8	1098.0
9	1411.0
10	2226.0
11	3041.0
12	4563.0
13	6085.0
14	8690.0
15	11295.0
16	17525.0
17	23755.0
18	34909.0
19	46063.0
20	64665.5
21	83268.0
22	432554.5
23	781841.0
24	540336.5
25	289961.0
26	281090.0
27	300344.5
28	319599.0
29	352875.0
30	386151.0
31	427243.5
32	468336.0
33	516670.0
34	565004.0
35	629474.0
36	693944.0
37	761030.5
38	828117.0
39	898104.5
40	968092.0
41	1033299.0
42	1171113.5
43	1243721.0
44	1301504.5
45	1359288.0
46	1402576.0
47	1445864.0
48	1474856.5
49	1503849.0
50	1479375.0
51	1454901.0
52	1397148.5
53	1339396.0
54	1269650.5
55	1199905.0
56	1176300.0
57	1152695.0
58	985762.0
59	697089.0
60	575349.0
61	490615.5
62	405882.0
63	340400.0
64	274918.0
65	223659.5
66	172401.0
67	135889.5
68	99378.0
69	77105.5
70	54833.0
71	41803.0
72	28773.0
73	21293.5
74	13814.0
75	10429.0
76	5065.5
77	3087.0
78	2277.5
79	1468.0
80	1171.5
81	875.0
82	655.0
83	435.0
84	308.0
85	181.0
86	130.5
87	80.0
88	56.0
89	32.0
90	29.5
91	27.0
92	22.0
93	11.5
94	6.0
95	5.0
96	4.0
97	2.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.0028846E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.15681278709052
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.88993776906878	38.017068382839994
2	14.501432151031185	15.12696963692058
3	5.272290817012591	8.249576553060667
4	2.415401879950162	5.039186546325886
5	1.3423350952188158	3.5005960129434555
6	0.8300760551001032	2.5976472842941636
7	0.542472167141467	1.9805533484663345
8	0.3888172451317923	1.622357461018494
9	0.2822342991227916	1.3248397351298835
>10	1.448408900966619	13.44564218166662
>50	0.06776888957002382	2.343134072618814
>100	0.01736049406450488	1.5370339260412151
>500	7.516562281889184E-4	0.2735613986313492
>1k	5.740901477621281E-4	0.6585629187768909
>5k	5.9352962292408616E-5	0.24290106725059238
>10k+	7.913728305654437E-5	4.04036947401474
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	571592	2.853843900941672	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	69365	0.34632549473893803	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	44100	0.22018243087994185	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA	25572	0.1276758531170493	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG	21098	0.10533807090034045	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.486317883716315E-5	0.0	0.0	0.0	9.985597772332964E-6
2	9.985597772332964E-5	9.985597772332964E-6	0.0	0.0	9.985597772332964E-6
3	1.1483437438182909E-4	9.985597772332964E-6	0.0	0.0	1.4978396658499447E-5
4	1.1483437438182909E-4	9.985597772332964E-6	0.0	0.0	1.4978396658499447E-5
5	1.1483437438182909E-4	9.985597772332964E-6	0.0	0.0	1.4978396658499447E-5
6	1.1982717326799558E-4	9.985597772332964E-6	0.0	4.992798886166482E-6	1.4978396658499447E-5
7	1.2481997215416205E-4	9.985597772332964E-6	0.0	4.992798886166482E-6	1.4978396658499447E-5
8	1.2981277104032855E-4	9.985597772332964E-6	0.0	9.985597772332964E-6	1.4978396658499447E-5
9	1.2981277104032855E-4	9.985597772332964E-6	0.0	1.9971195544665928E-5	1.4978396658499447E-5
10	1.2981277104032855E-4	9.985597772332964E-6	0.0	1.9971195544665928E-5	1.4978396658499447E-5
11	1.397983688126615E-4	1.9971195544665928E-5	0.0	1.9971195544665928E-5	1.4978396658499447E-5
12	1.4978396658499446E-4	1.9971195544665928E-5	0.0	2.496399443083241E-5	1.4978396658499447E-5
13	1.4978396658499446E-4	2.496399443083241E-5	0.0	2.9956793316998894E-5	1.4978396658499447E-5
14	1.647623632434939E-4	2.496399443083241E-5	0.0	4.493518997549834E-5	1.4978396658499447E-5
15	1.697551621296604E-4	2.9956793316998894E-5	0.0	4.493518997549834E-5	1.4978396658499447E-5
16	1.7474796101582687E-4	3.494959220316537E-5	0.0	4.992798886166482E-5	1.4978396658499447E-5
17	1.7974075990199336E-4	3.494959220316537E-5	0.0	4.992798886166482E-5	1.9971195544665928E-5
18	2.0470475433282577E-4	3.494959220316537E-5	0.0	4.992798886166482E-5	1.9971195544665928E-5
19	2.0969755321899224E-4	4.992798886166482E-5	0.0	4.992798886166482E-5	1.9971195544665928E-5
20	2.2467594987749169E-4	4.992798886166482E-5	0.0	5.4920787747831303E-5	2.496399443083241E-5
21	2.3466154764982465E-4	5.4920787747831303E-5	0.0	5.991358663399779E-5	2.496399443083241E-5
22	2.446471454221576E-4	6.490638552016427E-5	0.0	5.991358663399779E-5	2.496399443083241E-5
23	2.6961113985299003E-4	6.490638552016427E-5	0.0	5.991358663399779E-5	2.496399443083241E-5
24	3.095535309423219E-4	6.490638552016427E-5	0.0	5.991358663399779E-5	2.496399443083241E-5
25	3.345175253731543E-4	6.490638552016427E-5	0.0	5.991358663399779E-5	2.496399443083241E-5
26	3.6946711757631967E-4	6.490638552016427E-5	0.0	5.991358663399779E-5	2.9956793316998894E-5
27	3.9942391089331855E-4	6.490638552016427E-5	0.0	5.991358663399779E-5	2.9956793316998894E-5
28	4.743158941858158E-4	6.490638552016427E-5	0.0	6.490638552016427E-5	2.9956793316998894E-5
29	5.242438830474806E-4	6.490638552016427E-5	0.0	7.489198329249723E-5	2.9956793316998894E-5
30	5.941430674538114E-4	6.490638552016427E-5	0.0	8.48775810648302E-5	2.9956793316998894E-5
31	6.84013447404808E-4	7.988478217866371E-5	0.0	8.48775810648302E-5	2.9956793316998894E-5
32	7.988478217866371E-4	7.988478217866371E-5	0.0	8.987037995099668E-5	2.9956793316998894E-5
33	9.036965983961333E-4	8.987037995099668E-5	0.0	9.486317883716315E-5	3.494959220316537E-5
34	9.83581380574797E-4	8.987037995099668E-5	0.0	9.486317883716315E-5	3.494959220316537E-5
35	0.0010884301571842932	9.486317883716315E-5	0.0	9.985597772332964E-5	3.494959220316537E-5
36	0.001223235727110788	9.486317883716315E-5	0.0	1.0484877660949612E-4	3.494959220316537E-5
37	0.001378012492581949	9.486317883716315E-5	0.0	1.1483437438182909E-4	3.494959220316537E-5
38	0.001552760453597776	9.985597772332964E-5	0.0	1.1483437438182909E-4	4.493518997549834E-5
39	0.0016975516212966038	9.985597772332964E-5	0.0	1.1982717326799558E-4	4.493518997549834E-5
40	0.0018972635767432631	9.985597772332964E-5	0.0	1.1982717326799558E-4	4.493518997549834E-5
41	0.0021419107221654207	9.985597772332964E-5	0.0	1.2981277104032855E-4	4.493518997549834E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	272750	0.0	45.29232	1
GTGGTAT	278070	0.0	44.188316	6
AGTGGTA	278560	0.0	44.136738	5
TCAACGC	279335	0.0	44.104298	12
ATCAACG	280615	0.0	44.056377	11
CAACGCA	280305	0.0	44.051445	13
AGCAGTG	281025	0.0	44.002136	2
GCAGTGG	280680	0.0	43.990913	3
GTATCAA	280300	0.0	43.970066	9
GGTATCA	280350	0.0	43.939598	8
TGGTATC	279775	0.0	43.925743	7
TATCAAC	280970	0.0	43.884453	10
ACGCAGA	281540	0.0	43.852364	15
AACGCAG	282150	0.0	43.788376	14
CGCAGAG	282320	0.0	43.71456	16
AGAGTAC	271110	0.0	43.410553	19
CAGTGGT	283515	0.0	43.38608	4
CAGAGTA	273035	0.0	43.135475	18
GAGTACT	93510	0.0	43.10218	20
GCAGAGT	276765	0.0	42.748577	17
>>END_MODULE