Basic Statistics
Measure | Value |
---|---|
Filename | SRR522059_1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 35370644 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 47 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT | 91557 | 0.25885024881085006 | Illumina PCR Primer Index 1 (95% over 24bp) |
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 56802 | 0.16059079953421262 | No Hit |
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 48410 | 0.13686490978224766 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GGTATCA | 27965 | 0.0 | 32.094505 | 1 |
TATGCCG | 19905 | 0.0 | 30.908037 | 35 |
ACCGATA | 14730 | 0.0 | 29.674757 | 25 |
GACCGAT | 20755 | 0.0 | 29.514793 | 24 |
ATGCCGT | 20930 | 0.0 | 29.240406 | 36 |
GATATCG | 15005 | 0.0 | 29.028915 | 28 |
CGATATC | 14935 | 0.0 | 28.98943 | 27 |
GCCGTCT | 21260 | 0.0 | 28.812225 | 38 |
CCGATAT | 15260 | 0.0 | 28.451721 | 26 |
ATATCGT | 15380 | 0.0 | 27.980068 | 29 |
ATCGTAT | 15660 | 0.0 | 27.919983 | 31 |
TCGTATG | 22055 | 0.0 | 27.844742 | 32 |
TGAGCGG | 23670 | 0.0 | 27.82864 | 5 |
TGCCGTC | 22235 | 0.0 | 27.513676 | 37 |
CCGTCTT | 22275 | 0.0 | 27.49366 | 39 |
AGACCGA | 23130 | 0.0 | 27.34191 | 23 |
GCGGGCT | 24060 | 0.0 | 27.223398 | 8 |
TATCGTA | 16000 | 0.0 | 27.147987 | 30 |
CGTATGC | 22815 | 0.0 | 27.055658 | 33 |
AGCGGGC | 25635 | 0.0 | 25.66993 | 7 |