FastQCFastQC Report
Fri 27 May 2016
SRR522059_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522059_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35370644
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT915570.25885024881085006Illumina PCR Primer Index 1 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT568020.16059079953421262No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT484100.13686490978224766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA279650.032.0945051
TATGCCG199050.030.90803735
ACCGATA147300.029.67475725
GACCGAT207550.029.51479324
ATGCCGT209300.029.24040636
GATATCG150050.029.02891528
CGATATC149350.028.9894327
GCCGTCT212600.028.81222538
CCGATAT152600.028.45172126
ATATCGT153800.027.98006829
ATCGTAT156600.027.91998331
TCGTATG220550.027.84474232
TGAGCGG236700.027.828645
TGCCGTC222350.027.51367637
CCGTCTT222750.027.4936639
AGACCGA231300.027.3419123
GCGGGCT240600.027.2233988
TATCGTA160000.027.14798730
CGTATGC228150.027.05565833
AGCGGGC256350.025.669937