FastQCFastQC Report
Fri 27 May 2016
SRR522058_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522058_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34583252
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT1383540.4000606999017906Illumina PCR Primer Index 1 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT538030.1555753056421646No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT479550.13866538635522188No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT458030.13244272111830316Illumina Paired End PCR Primer 2 (96% over 29bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG274150.033.39720535
ACCGATA202100.032.74141325
GACCGAT281400.032.4606924
GATATCG203400.032.4067128
CGATATC203300.032.34781327
ATGCCGT284700.032.02968636
CCGATAT206350.031.95308726
GCCGTCT288550.031.54712738
ATATCGT206850.031.51550129
TGAGCGG312750.031.3574125
AGACCGA300050.031.28983323
GCGGGCT310450.031.2791048
TCGTATG291300.031.23764632
GGTATCA261450.031.2275091
ATCGTAT211400.031.18550331
TGCCGTC294950.030.8838537
TATCGTA212650.030.7816330
CCGTCTT295900.030.73642239
CGTATGC298400.030.70491233
AGCGGGC328700.029.6754367
Produced by FastQC (version 0.11.5)