##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR522058_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34583252
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.401274408780296	40.0	38.0	40.0	35.0	40.0
2	38.122866149198465	39.0	38.0	40.0	35.0	40.0
3	38.096128756196784	39.0	38.0	40.0	35.0	40.0
4	38.19056706986376	39.0	38.0	40.0	35.0	40.0
5	38.1440492640773	39.0	38.0	40.0	35.0	40.0
6	38.18368145367012	39.0	38.0	40.0	35.0	40.0
7	38.06571429430639	39.0	38.0	40.0	35.0	40.0
8	38.043551659051616	39.0	38.0	40.0	35.0	40.0
9	38.12119508020819	39.0	38.0	40.0	35.0	40.0
10	38.04626950640732	39.0	38.0	40.0	35.0	40.0
11	38.11873024549571	39.0	38.0	40.0	35.0	40.0
12	38.01346539070415	39.0	38.0	40.0	35.0	40.0
13	37.98776228447226	39.0	38.0	40.0	34.0	40.0
14	37.98752589837416	39.0	38.0	40.0	34.0	40.0
15	37.92756973809172	39.0	38.0	40.0	33.0	40.0
16	37.94591584388883	39.0	38.0	40.0	34.0	40.0
17	37.86920096467504	39.0	38.0	40.0	33.0	40.0
18	37.84226789892402	39.0	38.0	40.0	33.0	40.0
19	37.78527325885952	39.0	38.0	40.0	33.0	40.0
20	37.75123235952478	39.0	38.0	40.0	33.0	40.0
21	37.76664132106489	39.0	38.0	40.0	33.0	40.0
22	37.70888874186846	39.0	38.0	40.0	33.0	40.0
23	37.658794725261814	39.0	38.0	40.0	33.0	40.0
24	37.613060449028914	39.0	38.0	40.0	33.0	40.0
25	37.48464132291549	39.0	38.0	40.0	33.0	40.0
26	37.34968371395495	39.0	38.0	40.0	33.0	40.0
27	37.28873536820655	39.0	37.0	40.0	33.0	40.0
28	37.20068141654232	39.0	37.0	40.0	33.0	40.0
29	37.06664468685594	39.0	37.0	40.0	32.0	40.0
30	37.01650345086113	39.0	37.0	40.0	32.0	40.0
31	36.74257767892967	39.0	36.0	40.0	31.0	40.0
32	36.70396439293795	39.0	36.0	40.0	31.0	40.0
33	36.60031731544506	39.0	36.0	40.0	31.0	40.0
34	36.726063934068435	39.0	36.0	40.0	31.0	40.0
35	36.615238555356214	39.0	36.0	40.0	31.0	40.0
36	36.59618230812996	39.0	36.0	40.0	31.0	40.0
37	36.48159817358992	39.0	36.0	40.0	31.0	40.0
38	36.234599250527395	39.0	36.0	40.0	30.0	40.0
39	36.41790893464848	39.0	36.0	40.0	30.0	40.0
40	36.35850766723731	39.0	36.0	40.0	30.0	40.0
41	36.431895184408916	39.0	36.0	40.0	31.0	40.0
42	36.26027477115223	39.0	36.0	40.0	30.0	40.0
43	36.21212062416802	39.0	36.0	40.0	30.0	40.0
44	36.248610541310576	39.0	36.0	40.0	30.0	40.0
45	36.0024250466671	39.0	36.0	40.0	30.0	40.0
46	35.9315517233602	39.0	36.0	40.0	30.0	40.0
47	35.76778725147074	39.0	36.0	40.0	29.0	40.0
48	35.80904777260392	39.0	36.0	40.0	30.0	40.0
49	35.690850386192714	39.0	36.0	40.0	29.0	40.0
50	35.4326010173942	39.0	35.0	40.0	29.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1	1	-0.49393929582313945
1	2	-0.5080959401945506
1	3	-0.5071535172825818
1	4	-0.6020475061235189
1	5	-0.5888189526425478
1	6	-0.5644456835025551
1	7	-0.5685023923727357
1	8	-0.5980933648757585
1	9	-0.6358016486794043
1	10	-0.6223526579280474
1	11	-0.46466908068494917
1	12	-0.5744713224542437
1	13	-0.46854204666176
1	14	-0.6047976446738943
1	15	-0.5435688286700397
1	16	-0.5612173390817077
1	17	-0.583464736663089
1	18	-0.5358096065693445
1	19	-0.5536162543392393
1	20	-0.5616831578488046
1	21	-0.5157959720323788
1	22	-0.4998372793181005
1	23	-0.48497181681260315
1	24	-0.6007780943245535
1	25	-0.5177809502334654
1	26	-0.44144729228292334
1	27	-0.6247927514306966
1	28	-0.4778079418458603
1	29	-0.5762727956934128
1	30	-0.5023442690634212
1	31	-0.4247887981621048
1	32	-0.5431526752217408
1	33	-0.7039726778819002
1	34	-0.5644962486853728
1	35	-0.5573522259087866
1	36	-0.3730332185637337
1	37	-0.6756300548049907
1	38	-0.24319073758221066
1	39	-0.33146002056495405
1	40	-0.6338038677064475
1	41	-0.21820403933526222
1	42	-0.195867773189228
1	43	-0.21969306866973426
1	44	-0.4089806109171761
1	45	-0.22388147082865828
1	46	-0.27816242591469376
1	47	-0.36905585179195555
1	48	-0.47987749774590327
1	49	-0.35857240800603307
1	50	-0.3235666849449146
2	1	-0.42525871347365296
2	2	-0.46677001578062516
2	3	-0.434871668784524
2	4	-0.4323252806407112
2	5	-0.4230172500301421
2	6	-0.49637128962623933
2	7	-0.4513520722150801
2	8	-0.4798383675366438
2	9	-0.4613490432986751
2	10	-0.42147756456324004
2	11	-0.5755588751174656
2	12	-0.4935730740566555
2	13	-0.5287539786277904
2	14	-0.5577210188837256
2	15	-0.492464980268835
2	16	-0.44629211248958
2	17	-0.5531276588792409
2	18	-0.6028321304384434
2	19	-0.5107439926487487
2	20	-0.5449223867671051
2	21	-0.604831854660226
2	22	-0.5173154051852791
2	23	-0.4905124500891276
2	24	-0.5404197462080873
2	25	-0.5351224223819528
2	26	-0.6060796799683388
2	27	-0.4975436397525286
2	28	-0.5155865790618108
2	29	-0.6122913445169758
2	30	-0.5527454754341221
2	31	-0.6325138360098208
2	32	-0.6403137568190971
2	33	-0.8178684313511297
2	34	-0.5327263231643116
2	35	-0.6873498279848178
2	36	-0.7380742919677985
2	37	-0.5680789256439809
2	38	-1.0201924149094452
2	39	-0.7814854891700591
2	40	-0.9970115989100918
2	41	-1.0654008052324855
2	42	-1.169176030711526
2	43	-0.7672752046798408
2	44	-0.8722340525783352
2	45	-1.3038311322101848
2	46	-1.1487158676825615
2	47	-1.1095759578941227
2	48	-0.9914431388440121
2	49	-1.0029995732376307
2	50	-1.1276021892607488
3	1	-0.45839107250367306
3	2	-0.5437570031827832
3	3	-0.5356797780849263
3	4	-0.5026569407224244
3	5	-0.5030163675299519
3	6	-0.8378993030459512
3	7	-0.5053473431961422
3	8	-0.6119384683312106
3	9	-0.5385502194085134
3	10	-0.43703661977442465
3	11	-0.42293938525352814
3	12	-0.4628458765725725
3	13	-0.6713600689593306
3	14	-0.471213827271427
3	15	-0.4736290306349389
3	16	-0.5051899533091699
3	17	-0.4472088401332641
3	18	-0.529254858833518
3	19	-0.6410733415174832
3	20	-0.5371776363949223
3	21	-0.4820650107020512
3	22	-0.46409955919114054
3	23	-0.46168058176386495
3	24	-0.4706126051102686
3	25	-0.5001027357999632
3	26	-0.4686719254411642
3	27	-0.4662988385805349
3	28	-0.47592967190297486
3	29	-0.5261284196952971
3	30	-0.37889496505654563
3	31	-0.4245294633966381
3	32	-0.43470027906415964
3	33	-0.4293534988058383
3	34	-0.4877385360090898
3	35	-0.3547076947556178
3	36	-0.3405976209011996
3	37	-0.5054940866508417
3	38	-0.4882608125469119
3	39	-0.5723593997638261
3	40	-0.5656353439331667
3	41	-0.6916215144548872
3	42	-0.5566940417150548
3	43	-0.44455950497081176
3	44	-0.4880265382624174
3	45	-0.742519879588798
3	46	-0.3654725857152954
3	47	-0.40933910136389073
3	48	-0.4852289617535348
3	49	-0.49547767964086376
3	50	-0.639561075757463
4	1	-0.3761932412894069
4	2	-0.38804244626238216
4	3	-0.42099271217950474
4	4	-0.4204201643105634
4	5	-0.5044986235915516
4	6	-0.44484531580951625
4	7	-0.5869208400298191
4	8	-0.606257652969397
4	9	-0.4657507625568371
4	10	-0.6318295766797846
4	11	-0.5762492547344564
4	12	-0.5347555773433541
4	13	-0.5090499868214664
4	14	-0.6017763715309314
4	15	-0.4977146722272181
4	16	-0.4788459945904151
4	17	-0.5341201798656741
4	18	-0.4380760246528794
4	19	-0.5388364455795696
4	20	-0.5364417798272996
4	21	-0.4754056120869805
4	22	-0.44352963367606435
4	23	-0.45038022093041974
4	24	-0.46010023748733886
4	25	-0.5079438014777296
4	26	-0.6655946445163394
4	27	-0.5599273614941112
4	28	-0.5991792113547874
4	29	-0.7326632861563027
4	30	-0.5234006245589455
4	31	-0.7097447979821538
4	32	-0.7690419114400768
4	33	-0.925808128318657
4	34	-0.7259413875958955
4	35	-0.7782475120893224
4	36	-0.6221447659431334
4	37	-0.5839223020145923
4	38	-0.7909153933249442
4	39	-0.9319159852321377
4	40	-0.683426660236016
4	41	-0.8493798052711057
4	42	-1.213637795820837
4	43	-0.8331138733331116
4	44	-0.9399386133501366
4	45	-0.9881519976791324
4	46	-0.8098597755020407
4	47	-0.9853487606240208
4	48	-0.9021969769885558
4	49	-0.8961884983440243
4	50	-0.6582283897553083
5	1	-0.43010214211129494
5	2	-0.4822990458509864
5	3	-0.41625418616099097
5	4	-0.4245311502137099
5	5	-0.39871794031756025
5	6	-0.42551058591136837
5	7	-0.38307356484445876
5	8	-0.43459473919106983
5	9	-0.35054443502101407
5	10	-0.4910374062282443
5	11	-0.5429379129409853
5	12	-0.41304231443378114
5	13	-0.4792480512894457
5	14	-0.5230973541749435
5	15	-0.49004827568833065
5	16	-0.4588509811747983
5	17	-0.48429569470398093
5	18	-0.5239480070489293
5	19	-0.4293867625228387
5	20	-0.4699191406611547
5	21	-0.3928810727832115
5	22	-0.4222119643129716
5	23	-0.40459853920453526
5	24	-0.43096803991507215
5	25	-0.38180892352277596
5	26	-0.5452177664186948
5	27	-0.47339189580127794
5	28	-0.526363412259677
5	29	-0.5582701093608833
5	30	-0.4587621029941573
5	31	-0.4876362252801201
5	32	-0.5401672816130372
5	33	-0.5627570498731842
5	34	-0.5833984971351427
5	35	-0.612933573258367
5	36	-0.457312879903192
5	37	-0.5474849478947164
5	38	-0.3758916397632035
5	39	-0.7454366050296315
5	40	-0.5297204189463187
5	41	-0.5480163998321927
5	42	-0.8866231089605705
5	43	-0.4891449957873846
5	44	-0.6855857890320181
5	45	-0.78453460852554
5	46	-0.6330990819920146
5	47	-0.5330247026479569
5	48	-0.6317725546983013
5	49	-0.616114045758188
5	50	-0.46541821599298316
6	1	-0.366560261424425
6	2	-0.3761999860999765
6	3	-0.4079089307805148
6	4	-0.4017653349877932
6	5	-0.4291805698889348
6	6	-0.5045045923594813
6	7	-0.49775554308100567
6	8	-0.4652948726251438
6	9	-0.42569721551458883
6	10	-0.3524663109983308
6	11	-0.5846398870085636
6	12	-0.5072231059900929
6	13	-0.6878980059108812
6	14	-0.7265634859636592
6	15	-0.6068910993215226
6	16	-0.5317170290574822
6	17	-0.5462091200933656
6	18	-0.5091479297747341
6	19	-0.6330128165189635
6	20	-0.5732369590108632
6	21	-0.6686903862738163
6	22	-0.4959778908002974
6	23	-0.41269026236579975
6	24	-0.45626727867409755
6	25	-0.4659208387139202
6	26	-0.5395484197397735
6	27	-0.39568252813518967
6	28	-0.4938518050530689
6	29	-0.520957896072936
6	30	-0.4099925852852593
6	31	-0.5110822932658081
6	32	-0.7230186072940867
6	33	-0.5095131646911923
6	34	-0.6089986120934299
6	35	-0.595613448809722
6	36	-0.5729297945635068
6	37	-0.6243453148917766
6	38	-0.5917181394248061
6	39	-0.7013022222107139
6	40	-0.8387975693855338
6	41	-0.5486294094409843
6	42	-0.6251404880659379
6	43	-0.6967783042776787
6	44	-0.6734325342289367
6	45	-0.6286289651635428
6	46	-0.6508836000499585
6	47	-0.8614659635353732
6	48	-0.6027800200171143
6	49	-0.6162061735910527
6	50	-0.5115235293141609
7	1	-0.32699140546547767
7	2	-0.35604692754287726
7	3	-0.3220389106519761
7	4	-0.3824878689043061
7	5	-0.383953308946154
7	6	-0.3712675694024199
7	7	-0.44396525075978843
7	8	-0.5588044932476208
7	9	-0.3300176234974188
7	10	-0.399374608399377
7	11	-0.6017509343982326
7	12	-0.5670574038564737
7	13	-0.4370461953936271
7	14	-0.5980802071037061
7	15	-0.46896836364946637
7	16	-0.5203807865101595
7	17	-0.5098577269805986
7	18	-0.5136463905434141
7	19	-0.6108725108706992
7	20	-0.4708266458243813
7	21	-0.4770870439761268
7	22	-0.43423019847987376
7	23	-0.4036986761900536
7	24	-0.3919586916566189
7	25	-0.630161562834374
7	26	-0.4284142097626713
7	27	-0.5575930906512312
7	28	-0.5743704353943713
7	29	-0.5268799833991693
7	30	-0.47476953880488537
7	31	-0.565562260743512
7	32	-0.8188153090789214
7	33	-0.6448222641494823
7	34	-0.492457882362487
7	35	-0.6839300903490795
7	36	-0.4991937655518086
7	37	-0.47809805838299013
7	38	-0.4436832307248366
7	39	-0.7453191069927101
7	40	-0.6690043113983606
7	41	-0.6068504371984815
7	42	-0.5957113432994205
7	43	-0.6386951598069999
7	44	-0.69000486154264
7	45	-0.8779044713298632
7	46	-0.6156329510399701
7	47	-0.80720971524795
7	48	-0.645352653014541
7	49	-0.7928282274846694
7	50	-0.8540243453783845
8	1	-0.25602066231490994
8	2	-0.22934676690088196
8	3	-0.3059981625195505
8	4	-0.2865241741404887
8	5	-0.22993840569402124
8	6	-0.30375569817584847
8	7	-0.2767921482960105
8	8	-0.3265234941729105
8	9	-0.5045448108657951
8	10	-0.23993828425680874
8	11	-0.4014547205622634
8	12	-0.35457601580005615
8	13	-0.4801705965920533
8	14	-0.32565603835416823
8	15	-0.4813364794353916
8	16	-0.4138310737740767
8	17	-0.5263983216932218
8	18	-0.4512707879543427
8	19	-0.5347319822785366
8	20	-0.5390393606886263
8	21	-0.36296450508755385
8	22	-0.3543297693298797
8	23	-0.334263479996892
8	24	-0.37097125755268223
8	25	-0.4356654163848219
8	26	-0.4442658940650901
8	27	-0.49500279373489064
8	28	-0.4983510031192253
8	29	-0.3695138148046624
8	30	-0.33434419499563006
8	31	-0.5838678316825536
8	32	-0.33606032739746183
8	33	-0.42277115403246057
8	34	-0.5737133035499014
8	35	-0.5176792707179416
8	36	-0.4887186747114072
8	37	-0.41700366935541666
8	38	-0.5716635672563726
8	39	-0.7244209126768055
8	40	-1.1152056382279838
8	41	-0.7387758987316175
8	42	-0.7680300534482782
8	43	-0.6144391628154153
8	44	-0.8243568151728127
8	45	-0.9159617128429289
8	46	-0.5513492772620339
8	47	-0.7687625166146006
8	48	-0.6130653864012316
8	49	-0.621753167172578
8	50	-1.1369731019205176
9	1	-0.34081758967221987
9	2	-0.35642652216832715
9	3	-0.3728641687600174
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115	4	-0.24608874563340066
115	5	-0.19010267476788556
115	6	-0.1850994310112739
115	7	-0.19865723400718593
115	8	-0.20307894108566416
115	9	-0.22072241918328217
115	10	-0.3451514911697373
115	11	-0.19336053144314036
115	12	-0.2723350958846851
115	13	-0.31327510629676425
115	14	-0.29058337769033216
115	15	-0.16361168796095882
115	16	-0.17244372358589288
115	17	-0.1492400323790335
115	18	-0.18944506469739508
115	19	-0.22492697566243436
115	20	-0.16517995649756756
115	21	-0.11401880051670332
115	22	-0.17230547180081146
115	23	-0.15757316461083093
115	24	-0.1661807015895036
115	25	-0.14008264023689776
115	26	-0.24640572908291603
115	27	-0.17264898879827228
115	28	-0.14289282550972615
115	29	-0.16739567108463405
115	30	-0.32026451970530445
115	31	-0.3725311628681851
115	32	-0.14607749144595772
115	33	-0.1702120552453934
115	34	-0.09828625007686753
115	35	-0.10419929961975782
115	36	-0.2595990002346156
115	37	-0.13313301902790897
115	38	0.09167599354271516
115	39	-0.06494217570053706
115	40	-0.016902553004037202
115	41	-0.2124189990752825
115	42	0.07025995419073894
115	43	-0.183622317829915
115	44	-0.03559036452751485
115	45	-0.16360210205384362
115	46	-0.10995784496765992
115	47	-0.1352009829070866
115	48	-0.03488297225113968
115	49	-0.3146339664593114
115	50	0.1089930317497192
116	1	-0.21629769942911992
116	2	-0.24432290080570596
116	3	-0.24913368658369706
116	4	-0.2836470516859251
116	5	-0.24151934179560186
116	6	-0.2499089098483438
116	7	-0.3529293369499982
116	8	-0.29897744994414666
116	9	-0.3389433302821274
116	10	-0.4027374285027463
116	11	-0.2170433233044733
116	12	-0.216016198714982
116	13	-0.26060594408654225
116	14	-0.24892490884133878
116	15	-0.2529496893387915
116	16	-0.2273732982728447
116	17	-0.2149285442137696
116	18	-0.24781808338004652
116	19	-0.258276420999735
116	20	-0.22801853507426273
116	21	-0.2375083401297431
116	22	-0.17641959849808586
116	23	-0.20211624034699582
116	24	-0.18481695294769196
116	25	-0.20240376519910797
116	26	-0.21037631638156284
116	27	-0.19035456864413902
116	28	-0.21587530309215452
116	29	-0.2477035798010121
116	30	-0.1724695250703263
116	31	-0.19238425930039682
116	32	-0.1515024475095501
116	33	-0.2184211134672509
116	34	-0.1620865472502544
116	35	-0.16349788501764806
116	36	-0.025534586330238085
116	37	-0.06154243998638975
116	38	-0.08363755111002291
116	39	0.125379747775348
116	40	-0.0016628674400749333
116	41	-0.1293444673135511
116	42	-0.0270998212873792
116	43	-0.14532808723277668
116	44	-0.032218455830658854
116	45	5.484826234365414E-4
116	46	-0.1284889605977071
116	47	-0.06625868438942462
116	48	-0.09610900751198415
116	49	-0.08062098002587703
116	50	-0.141241112724515
117	1	-0.2970691571428574
117	2	-0.3805448877933202
117	3	-0.3550280598250808
117	4	-0.3911013677149455
117	5	-0.33338003325455645
117	6	-0.356261311461715
117	7	-0.4897752638446846
117	8	-0.5102515132057448
117	9	-0.4270686363960934
117	10	-0.3998545729659213
117	11	-0.41462887147178407
117	12	-0.5413064146226176
117	13	-0.4653927214810807
117	14	-0.4729560612782393
117	15	-0.4813605630771818
117	16	-0.5413808406878857
117	17	-0.4091371414075482
117	18	-0.46599864118458356
117	19	-0.45275333520988426
117	20	-0.4494012213850027
117	21	-0.5395474545358567
117	22	-0.3922378575328409
117	23	-0.38830072529467685
117	24	-0.4392372413916448
117	25	-0.4432955746396061
117	26	-0.42210500234568826
117	27	-0.45028090959488054
117	28	-0.4946557775834677
117	29	-0.6054648189762872
117	30	-0.502995253481231
117	31	-0.510559819085671
117	32	-0.457910726401181
117	33	-0.46513781935308884
117	34	-0.44708363282108365
117	35	-0.46683106649431494
117	36	-0.4756791448493374
117	37	-0.3601366855614785
117	38	-0.4865552412920522
117	39	-0.43656741302375934
117	40	-0.30054325569277296
117	41	-0.3263239392023465
117	42	-0.5264517132766713
117	43	-0.44758125764622747
117	44	-0.3759955188245243
117	45	-0.49960272796163707
117	46	-0.3650077651573085
117	47	-0.33399595482751465
117	48	-0.30701437762968453
117	49	-0.27799916392769575
117	50	-0.4551519352658673
118	1	-0.2690994716473938
118	2	-0.3091253376122509
118	3	-0.28748559370869486
118	4	-0.3194557461502825
118	5	-0.2943408480578995
118	6	-0.3275080554117835
118	7	-0.3894688362672696
118	8	-0.2652788039874707
118	9	-0.2957416875071033
118	10	-0.34084674800540427
118	11	-0.2822388675505252
118	12	-0.42939802074522504
118	13	-0.38710983718893743
118	14	-0.32547784066581187
118	15	-0.35966958449524356
118	16	-0.44553300026146303
118	17	-0.4442033559414611
118	18	-0.38748756847947874
118	19	-0.3660822507902708
118	20	-0.5038707864602188
118	21	-0.5112625551168506
118	22	-0.5113233315864392
118	23	-0.40516231720036444
118	24	-0.4463425506119876
118	25	-0.5394604089123618
118	26	-0.546054895577484
118	27	-0.47170398514033707
118	28	-0.6145303276237044
118	29	-0.43978990062995393
118	30	-0.4381193794596854
118	31	-0.43335516347494263
118	32	-0.42446071643226446
118	33	-0.4551496188927473
118	34	-0.5125935003142743
118	35	-0.4657998227660656
118	36	-0.6734796314625058
118	37	-0.545535770120992
118	38	-0.735201221857892
118	39	-1.0275124580579487
118	40	-0.5847002080359331
118	41	-0.7491856396525094
118	42	-0.752112919051612
118	43	-0.614890135479996
118	44	-0.6233220680553515
118	45	-0.876318136735101
118	46	-0.5344528373584296
118	47	-0.5437356067927368
118	48	-0.5757635439410151
118	49	-0.4745730102022563
118	50	-0.661997629263368
119	1	-0.30315394955034947
119	2	-0.328643312003706
119	3	-0.3651621261802305
119	4	-0.4566251195718536
119	5	-0.41747931816337314
119	6	-0.37233826267426195
119	7	-0.6590473271084392
119	8	-0.45678643923159257
119	9	-0.4868804600317489
119	10	-0.5303613394330853
119	11	-0.5030335983219274
119	12	-0.5484520425198554
119	13	-0.6501582346643389
119	14	-0.49062675714037596
119	15	-0.5271631327602435
119	16	-0.41491851733073304
119	17	-0.4368191945399573
119	18	-0.5042286966594247
119	19	-0.4932680913016583
119	20	-0.6582756165217774
119	21	-0.4553359518823541
119	22	-0.44196930391039757
119	23	-0.4106426746486491
119	24	-0.411039340959654
119	25	-0.6114274575994187
119	26	-0.5292855222505821
119	27	-0.5563014156545734
119	28	-0.5976124405532772
119	29	-0.46954137075697844
119	30	-0.5414328984723156
119	31	-0.4370502705032919
119	32	-0.5313230483252482
119	33	-0.5769930372629091
119	34	-0.360286722008361
119	35	-0.4859308557948623
119	36	-0.5512537874607233
119	37	-0.7203158724243153
119	38	-0.7148788937525907
119	39	-0.7129424481787581
119	40	-0.9750524194016492
119	41	-1.1973257048938422
119	42	-1.1450011352154448
119	43	-1.2371731838562283
119	44	-1.0793148746954628
119	45	-1.0360243220909382
119	46	-1.0643241079176633
119	47	-1.3994330565594382
119	48	-0.8140762722985002
119	49	-0.7962211832508643
119	50	-1.2113245148361855
120	1	-0.3914664325533792
120	2	-0.35915189074550824
120	3	-0.37413794549956236
120	4	-0.3482824815830554
120	5	-0.3708314798750152
120	6	-0.4132305778372398
120	7	-0.4025660963877016
120	8	-0.5053911700683358
120	9	-0.4897340650727102
120	10	-0.43209301245630627
120	11	-0.5068267289959962
120	12	-0.49798724644941217
120	13	-0.8409101265187218
120	14	-0.5081869903744973
120	15	-0.6269857951867621
120	16	-0.5850288207523207
120	17	-0.669688569993852
120	18	-0.6773102440306147
120	19	-0.5486168834002996
120	20	-0.7769513763457994
120	21	-0.5083141078119766
120	22	-0.4773563516891244
120	23	-0.41673340452231855
120	24	-0.4465767871391577
120	25	-0.5454613404145547
120	26	-0.6115491888002111
120	27	-0.5650587229117008
120	28	-0.6485748409453436
120	29	-0.7780048289878891
120	30	-0.8930630428038526
120	31	-0.657900692136586
120	32	-0.6227465018353584
120	33	-0.7231983517174285
120	34	-0.664642278076272
120	35	-0.5312369741418905
120	36	-0.4985348506144618
120	37	-0.4201382912491525
120	38	-0.2991010007564441
120	39	-0.4825361111327524
120	40	-0.3143678360654718
120	41	-0.6634130054982208
120	42	-0.8117662467583884
120	43	-0.6542112144166268
120	44	-0.5162786123930303
120	45	-0.7246565948936095
120	46	-0.6680042310173491
120	47	-0.6245818356572244
120	48	-0.4770323019977383
120	49	-0.4424806405491921
120	50	-0.8731311325453817
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	22467.0
3	1543.0
4	2426.0
5	2601.0
6	3994.0
7	6612.0
8	7318.0
9	8776.0
10	12479.0
11	17110.0
12	19403.0
13	24624.0
14	30202.0
15	36121.0
16	41290.0
17	46528.0
18	54777.0
19	65629.0
20	79082.0
21	74492.0
22	92402.0
23	114263.0
24	141640.0
25	155549.0
26	201693.0
27	251338.0
28	302378.0
29	352380.0
30	436483.0
31	533129.0
32	653768.0
33	813042.0
34	1096292.0
35	1554496.0
36	2293925.0
37	3658666.0
38	5399590.0
39	1.5838939E7
40	135805.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.568476560139445	19.029213237707012	11.102567289903291	25.299742912250256
2	18.247448028520648	21.914208278487244	34.713532304210055	25.12481138878205
3	19.42022398587617	24.24960787377659	30.95492002892036	25.375248111426885
4	13.490570522401999	16.21257017703251	36.24866163540664	34.048197665158845
5	14.663771353833353	37.64755552774505	34.883688786699416	12.804984331722189
6	33.93560270156202	36.109952875455434	16.58693925024749	13.367505172735056
7	28.71987284480939	32.173527232198985	21.477789306800876	17.62881061619075
8	28.503351853666047	33.60932627157215	19.983913022407492	17.903408852354314
9	27.334257055987678	12.801046009207	18.640297332361918	41.2243996024434
10	15.51401203826352	26.96421013574008	35.70779846542372	21.813979360572684
11	36.324432066859316	21.1719366633448	22.674732376746178	19.828898893049708
12	21.179584268130714	25.66710614721831	31.87280941653492	21.28050016811606
13	30.19689715524564	20.019866853392998	25.239419496708692	24.543816494652667
14	21.34926172358792	20.022656363156933	27.927266920914594	30.700814992340554
15	24.582824079123615	28.039604256997	23.29178007898158	24.0857915848978
16	23.979066305742766	26.395469254952232	25.591044503878745	24.03441993542626
17	22.58365855605766	26.82241906718758	26.83940996476818	23.754512411986585
18	23.069133178634164	26.52403692539018	27.421081014653048	22.985748881322607
19	23.683505969856256	26.774267395626445	27.21274668906061	22.329479945456693
20	24.19652290871218	25.790188544112784	27.244069246646802	22.76921930052824
21	24.22823055228434	25.566300266820484	27.054632530932572	23.150836649962603
22	23.38844586129483	25.722503430358213	27.08849053337375	23.80056017497321
23	23.35117397423929	26.094421189101812	27.15158730773764	23.402817528921258
24	23.61925260977226	25.783783303906194	27.40698612999879	23.189977956322757
25	23.403979874615974	26.0850376012119	27.202730545332948	23.308251978839177
26	23.2465483347646	25.673435863589145	27.277758118737726	23.80225768290852
27	23.2971332220031	25.853977392044687	27.03231246109884	23.816576924853365
28	23.592389697637884	25.692870704812222	27.264084447666093	23.450655149883808
29	22.912302241499873	26.59879433752795	27.307931956736205	23.180971464235974
30	23.22514685640555	25.933473000261948	27.77838156516978	23.06299857816272
31	23.378508420162067	26.239355985324075	27.193196701524393	23.18893889298947
32	22.998549280622242	26.00955058853969	27.690895664600536	23.301004466237536
33	23.02746968362734	25.837538458151	27.201486597029266	23.93350526119239
34	23.747410067128648	25.84882016649556	27.479966179973243	22.92380358640255
35	23.32070826557655	26.062426624492613	27.769101978713078	22.84776313121776
36	23.34286083014046	26.60509841980617	27.03247798973189	23.019562760321488
37	23.301573451814495	25.906389352466956	27.630157588712144	23.1618796070064
38	23.84504045513234	26.065592343045285	27.04713568363054	23.042231518191837
39	22.85885626030538	26.180837607244083	27.077033628519775	23.883272503930762
40	23.219925240563104	25.957469253930327	27.320779552105684	23.501825953400882
41	23.487117049313987	26.050245053746718	27.189029519170678	23.27360837776862
42	23.21203588916065	26.083364893754453	27.663694706055153	23.040904511029744
43	23.05242702828197	25.70614822094182	27.155991052471485	24.085433698304726
44	22.773807883677293	25.918295521173352	27.84996198086404	23.457934614285314
45	22.694022766845833	25.857843727756208	27.818545627420022	23.629587877977936
46	22.799129137270036	25.737425724707208	27.19267248569508	24.270772652327675
47	22.778562774916335	25.969794368168913	27.999025680970906	23.252617175943843
48	23.38140015436099	26.02954350242614	27.124234457351427	23.464821885861447
49	22.60700291261493	25.932133760769283	27.43903770464061	24.021825621975175
50	22.58560291957064	25.848102273275202	28.058990361629167	23.507304445524994
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10949.0
1	7765.5
2	4582.0
3	10520.0
4	16458.0
5	19102.0
6	21746.0
7	19080.0
8	16414.0
9	20211.5
10	24009.0
11	35715.0
12	47421.0
13	79550.0
14	111679.0
15	119877.5
16	128076.0
17	168455.5
18	208835.0
19	205313.5
20	201792.0
21	229456.0
22	257120.0
23	299631.0
24	342142.0
25	406407.5
26	470673.0
27	541680.0
28	612687.0
29	689527.0
30	766367.0
31	847420.5
32	928474.0
33	1032198.5
34	1135923.0
35	1235997.0
36	1336071.0
37	1443780.5
38	1551490.0
39	1641629.5
40	1731769.0
41	1832721.0
42	1933673.0
43	1975614.0
44	2017555.0
45	2087820.5
46	2158086.0
47	2210447.5
48	2262809.0
49	2299561.5
50	2336314.0
51	2314027.5
52	2291741.0
53	2232070.5
54	2172400.0
55	2107450.0
56	2042500.0
57	2029253.5
58	2016007.0
59	1798401.0
60	1580795.0
61	1370766.0
62	1160737.0
63	1041899.0
64	923061.0
65	764138.0
66	605215.0
67	506563.5
68	407912.0
69	344096.5
70	280281.0
71	225420.5
72	170560.0
73	145930.5
74	121301.0
75	94606.0
76	67911.0
77	55245.5
78	42580.0
79	35075.0
80	27570.0
81	22115.5
82	16661.0
83	14040.5
84	11420.0
85	9246.5
86	7073.0
87	5026.0
88	2979.0
89	1985.5
90	992.0
91	616.5
92	241.0
93	152.5
94	64.0
95	46.0
96	28.0
97	27.5
98	27.0
99	54.5
100	82.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13756080544420748
2	0.0025185601400354134
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	7.228932663706697E-5
11	8.906045041686653E-4
12	0.0
13	5.783146130965358E-5
14	0.0013677140599733073
15	0.0
16	4.4530225208433264E-4
17	8.38556188989977E-5
18	5.783146130965359E-6
19	2.6313314895892384E-4
20	0.002431812948070933
21	0.0013012078794672056
22	3.730129254472656E-4
23	1.1566292261930716E-4
24	0.006827004007604606
25	0.0024751865440531735
26	0.00591326691891208
27	0.019775468194836044
28	0.02941018964902433
29	0.10391735282731653
30	0.12152703279610605
31	0.12554631935712698
32	0.1327347699979169
33	0.19326406897766582
34	0.26047290173867976
35	0.23649308630663188
36	0.22716487159738477
37	0.26220784557796933
38	0.2558868668568242
39	0.28577994920778416
40	0.2825240379360507
41	0.31452796862481297
42	0.34772901056268507
43	0.34858202461700244
44	0.38981296495772
45	0.38350933567496776
46	0.397331054927975
47	0.40100913586726894
48	0.43656102670737845
49	0.41666411244379215
50	0.45187190608910927
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3.4583252E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.543552006358794
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.81354949385667	42.46230940648909
2	9.765069173188712	10.261828398942644
3	3.3558794744851537	5.289894830840481
4	1.7513938661422017	3.6809781875704224
5	1.016648319287227	2.670915691832284
6	0.6483867164266518	2.0441124692877595
7	0.450130137505244	1.6556005402745357
8	0.3292137678197246	1.3838448584516025
9	0.2417298914896007	1.1431212412477774
>10	1.466770508942673	14.136503640827192
>50	0.09598320182102679	3.459912952225544
>100	0.0570455237474876	5.693300621612227
>500	0.0050597519407364995	1.8124964341391887
>1k	0.002986176353697746	2.7077182403855926
>5k	7.149860563920364E-5	0.23342963377336884
>10k+	8.249838775551222E-5	1.3640328521001994
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCTGAGCGGGCTGGCAAGGCAGACCGATATCGTATGCCGTCTTCTGCT	138354	0.4000606999017906	Illumina PCR Primer Index 1 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53803	0.1555753056421646	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47955	0.13866538635522188	No Hit
TCTCTGAGCGGGCTGGCAAGGCAGACCGATCTCGTATGCCGTCTTCTGCT	45803	0.13244272111830316	Illumina Paired End PCR Primer 2 (96% over 29bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.891573065482679E-6	5.783146130965358E-6
2	0.0	3.759044985127483E-5	0.0	2.6024157589344115E-5	5.783146130965358E-6
3	0.0	3.759044985127483E-5	0.0	2.6024157589344115E-5	5.783146130965358E-6
4	0.0	8.38556188989977E-5	0.0	3.469887678579215E-5	8.674719196448037E-6
5	8.674719196448037E-6	8.38556188989977E-5	0.0	3.469887678579215E-5	8.674719196448037E-6
6	2.6024157589344115E-5	8.38556188989977E-5	0.0	3.469887678579215E-5	1.4457865327413396E-5
7	3.469887678579215E-5	8.963876502996306E-5	0.0	5.204831517868823E-5	1.7349438392896074E-5
8	4.626516904772287E-5	9.831348422641109E-5	0.0	5.493988824417091E-5	2.0241011458378756E-5
9	4.626516904772287E-5	2.3132584523861434E-4	0.0	8.963876502996306E-5	2.0241011458378756E-5
10	5.204831517868823E-5	2.3132584523861434E-4	0.0	1.2144606875027253E-4	2.0241011458378756E-5
11	5.493988824417091E-5	2.3132584523861434E-4	0.0	1.3301236101220324E-4	2.0241011458378756E-5
12	5.7831461309653585E-5	2.371089913695797E-4	0.0	1.648196647325127E-4	2.6024157589344115E-5
13	6.650618050610162E-5	2.400005644350624E-4	0.0	1.9951854151830487E-4	3.180730372030947E-5
14	1.3012078794672057E-4	3.122898910721294E-4	0.0	2.6024157589344115E-4	5.204831517868823E-5
15	1.4747022633961664E-4	3.267477563995428E-4	0.0	2.8337416041730257E-4	6.072303437513626E-5
16	1.792775300599261E-4	3.325309025305081E-4	0.0	3.036151718756813E-4	7.518089970254967E-5
17	1.8795224925637416E-4	3.354224755959908E-4	0.0	3.469887678579215E-4	7.518089970254967E-5
18	1.8795224925637416E-4	3.383140486614735E-4	0.0	3.8457921770919634E-4	8.096404583351502E-5
19	1.8795224925637416E-4	4.048202291675751E-4	0.0	4.3662753288788457E-4	8.674719196448038E-5
20	1.9662696845282218E-4	4.0771180223305776E-4	0.0	4.800011288701248E-4	8.674719196448038E-5
21	1.9951854151830487E-4	4.1349494836402314E-4	0.0	5.262662979178477E-4	8.963876502996306E-5
22	2.0241011458378756E-4	4.5397697128078066E-4	0.0	5.696398939000878E-4	8.963876502996306E-5
23	2.0530168764927023E-4	4.626516904772287E-4	0.0	6.303629282752241E-4	9.831348422641109E-5
24	2.1397640684571827E-4	4.915674211320555E-4	0.0	6.85302816519395E-4	9.831348422641109E-5
25	2.2554269910764899E-4	5.349410171142957E-4	0.0	7.344595586326006E-4	1.0120505729189378E-4
26	2.3421741830409703E-4	5.349410171142957E-4	0.0	8.125320314006329E-4	1.0409663035737646E-4
27	2.3421741830409703E-4	5.378325901797783E-4	0.0	0.0010814483264905222	1.0409663035737646E-4
28	2.515668566969931E-4	5.378325901797783E-4	0.0	0.0016742208049144714	1.0409663035737646E-4
29	2.515668566969931E-4	5.378325901797783E-4	0.0	0.0025908494666724807	1.0409663035737646E-4
30	2.5445842976247576E-4	5.927724784239493E-4	0.0	0.0038024185811097234	1.0409663035737646E-4
31	2.660247220244065E-4	5.95664051489432E-4	0.0	0.00507181915685662	1.0409663035737646E-4
32	2.7180786815537186E-4	5.95664051489432E-4	0.0	0.0069282090648965	1.0698820342285914E-4
33	2.7180786815537186E-4	5.95664051489432E-4	0.0	0.010675687757762052	1.0698820342285914E-4
34	2.7469944122085455E-4	5.95664051489432E-4	0.0	0.01725401648173515	1.0987977648834181E-4
35	2.7469944122085455E-4	7.980741660732195E-4	0.0	0.026561990179523893	1.0987977648834181E-4
36	2.7469944122085455E-4	8.009657391387021E-4	0.0	0.044628538692659675	1.0987977648834181E-4
37	2.7469944122085455E-4	8.125320314006329E-4	0.0	0.08105657617161047	1.0987977648834181E-4
38	2.7469944122085455E-4	8.414477620554597E-4	0.0	0.1441391341681806	1.0987977648834181E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	27415	0.0	33.397205	35
ACCGATA	20210	0.0	32.741413	25
GACCGAT	28140	0.0	32.46069	24
GATATCG	20340	0.0	32.40671	28
CGATATC	20330	0.0	32.347813	27
ATGCCGT	28470	0.0	32.029686	36
CCGATAT	20635	0.0	31.953087	26
GCCGTCT	28855	0.0	31.547127	38
ATATCGT	20685	0.0	31.515501	29
TGAGCGG	31275	0.0	31.357412	5
AGACCGA	30005	0.0	31.289833	23
GCGGGCT	31045	0.0	31.279104	8
TCGTATG	29130	0.0	31.237646	32
GGTATCA	26145	0.0	31.227509	1
ATCGTAT	21140	0.0	31.185503	31
TGCCGTC	29495	0.0	30.88385	37
TATCGTA	21265	0.0	30.78163	30
CCGTCTT	29590	0.0	30.736422	39
CGTATGC	29840	0.0	30.704912	33
AGCGGGC	32870	0.0	29.675436	7
>>END_MODULE