FastQCFastQC Report
Fri 27 May 2016
SRR522057_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522057_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28511972
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT9600.010.8319671
CGCCAAT20300.010.7899621
CGCCGAT11350.010.5263691
CTCGAAT39850.010.326791
CGATAAC14450.010.258591
CGCAAAT15800.09.9421881
CGAATGT32850.09.7103953
CGCAGAT33650.09.2707471
CGCCCAT31950.09.2100391
CGACAAT21200.09.18390751
CGGCAAT23200.09.1551181
GTTACCT76250.09.11109351
CGGAAAT33150.09.0101281
CGAAAAT27800.08.9932021
CCGGAAT29300.08.9858681
CGCGGTT11550.08.8116361
CGAAAAC22000.08.749381
CGGGCAT41750.08.743961
CGCGAAC6107.2759576E-128.7048661
AATCGAA20100.08.6463835
Produced by FastQC (version 0.11.5)