FastQCFastQC Report
Fri 27 May 2016
SRR522056_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522056_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31943008
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAT22600.010.9618951
CGCAAAT19950.010.5331131
CGCCACT50050.010.2974071
CGGCAAT24600.010.16061
CGCGGAT11600.010.1063391
CGCCAAT21700.010.0914011
CGATAAC16850.09.845491
CGGGAAT32800.09.5087031
CGCAGAT37500.09.3196981
CGCGGTT13450.09.2096211
CGCGTGT14500.09.1529111
CGGACAT38900.08.9842841
CGACGAT16400.08.766891
CGAATGT34900.08.7622173
CGCCGGT15500.08.7051081
CGGCGCT27750.08.6883951
CGAGAAT32350.08.6837131
CGGCGAT12550.08.6363131
CGCATAT10600.08.555671
TTAGTCG19850.08.53313424
Produced by FastQC (version 0.11.5)