FastQCFastQC Report
Fri 27 May 2016
SRR522055_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522055_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39462287
Sequences flagged as poor quality0
Sequence length36
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3242610.8216984484452206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA42650.011.37810921
AGATCGG42250.011.28452920
CGGTTCA48300.011.0579331
CGAGATC45750.010.41909418
GAGATCG47450.010.07885719
AATGCCG54000.09.789556513
AGCGGTT50200.08.98196230
GAGCGGT54750.08.31653529
ATGCCGA65250.08.02523314
ATCGGAA61600.08.02415122
CCGAGAT63100.07.927739617
TGCCGAG68900.07.451782715
GCCGAGA70400.07.315318616
TCGGAAG70600.07.06530523
AAGAGCG66050.06.98364727
AGTGGTA126400.06.85727022
TCAACGC133250.06.8189999
GGTATCA136200.06.58848145
CAACGCA136250.06.57830810
ATCAACG141800.06.4763038
Produced by FastQC (version 0.11.5)