##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR522055_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 39462287 Sequences flagged as poor quality 0 Sequence length 36 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.854280923961653 33.0 12.0 38.0 2.0 39.0 2 25.854176925934375 33.0 2.0 38.0 2.0 39.0 3 25.553261801577793 33.0 2.0 38.0 2.0 39.0 4 25.365898230885605 33.0 2.0 38.0 2.0 39.0 5 25.134254459200502 33.0 2.0 38.0 2.0 39.0 6 24.55423528798521 33.0 2.0 38.0 2.0 39.0 7 24.069929474690607 33.0 2.0 38.0 2.0 39.0 8 24.02750028147127 33.0 2.0 38.0 2.0 39.0 9 23.770060133615672 33.0 2.0 38.0 2.0 39.0 10 23.750104371801868 32.0 2.0 38.0 2.0 39.0 11 23.684077889352942 32.0 2.0 38.0 2.0 39.0 12 23.52750799769917 32.0 2.0 38.0 2.0 39.0 13 23.423911543697404 32.0 2.0 38.0 2.0 39.0 14 23.310004435373955 31.0 2.0 38.0 2.0 39.0 15 23.180021953618652 31.0 2.0 38.0 2.0 39.0 16 23.036811779307165 31.0 2.0 38.0 2.0 39.0 17 22.863238919731135 31.0 2.0 38.0 2.0 39.0 18 22.717693325782157 31.0 2.0 38.0 2.0 39.0 19 22.564809535747383 31.0 2.0 38.0 2.0 39.0 20 22.323194851834106 31.0 2.0 38.0 2.0 39.0 21 22.188202878358265 31.0 2.0 38.0 2.0 39.0 22 22.00000349700969 30.0 2.0 38.0 2.0 39.0 23 21.85296181642995 30.0 2.0 38.0 2.0 39.0 24 21.614971859081557 29.0 2.0 37.0 2.0 39.0 25 21.525154054046588 29.0 2.0 37.0 2.0 39.0 26 21.235592478459242 29.0 2.0 37.0 2.0 39.0 27 21.007231258543126 29.0 2.0 36.0 2.0 39.0 28 20.937940570955757 29.0 2.0 36.0 2.0 39.0 29 20.773350186217034 28.0 2.0 36.0 2.0 39.0 30 20.53235072260257 27.0 2.0 36.0 2.0 39.0 31 20.441446183795684 27.0 2.0 36.0 2.0 39.0 32 20.198100353382966 27.0 2.0 36.0 2.0 39.0 33 19.975801579872957 27.0 2.0 36.0 2.0 39.0 34 19.751291302503578 26.0 2.0 35.0 2.0 38.0 35 19.50925961285518 25.0 2.0 35.0 2.0 38.0 36 19.417909838829157 25.0 2.0 36.0 2.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -17.211903604368697 1 2 -17.87233778131278 1 3 -17.41023839911422 1 4 -17.247628659323983 1 5 -17.02287903512071 1 6 -14.546225906330221 1 7 -14.096780456517532 1 8 -14.08982441481811 1 9 -13.829219693816611 1 10 -13.820235787800595 1 11 -10.380585975199004 1 12 -10.293751063136655 1 13 -10.208228782385694 1 14 -10.156124573999003 1 15 -10.153187565383671 1 16 -10.836765372374874 1 17 -10.75580058961166 1 18 -10.639122553938805 1 19 -10.571075649966422 1 20 -10.483810849380756 1 21 -10.927836502278678 1 22 -10.780264898413185 1 23 -10.676900131930287 1 24 -10.459165704628692 1 25 -10.526091036764129 1 26 -10.693397891013065 1 27 -10.539953344109314 1 28 -10.44842627174836 1 29 -10.337897687912339 1 30 -10.175122196525976 1 31 -10.30618032491039 1 32 -10.098713560088209 1 33 -9.960155430903232 1 34 -9.783846061438306 1 35 -9.566840025803451 1 36 -9.515774230003828 2 1 1.1252370595738945 2 2 1.1290308105027975 2 3 1.2247449433671278 2 4 1.1288481108168966 2 5 1.0773657738890705 2 6 1.1236601459964248 2 7 1.2650812824184534 2 8 1.178613844213011 2 9 1.3690776532590725 2 10 1.1081928922425028 2 11 1.0234999754830305 2 12 0.9591664380322555 2 13 0.9456635475120514 2 14 0.9407291106181042 2 15 0.8312401388711699 2 16 0.9876034430588447 2 17 1.0007154072170295 2 18 0.9668281443596634 2 19 0.9307612551584974 2 20 1.13770333004231 2 21 1.046425293578288 2 22 0.8748231136215381 2 23 1.0315854853574287 2 24 1.022864780622676 2 25 0.9748115348444983 2 26 0.9265073436843387 2 27 0.958541242298768 2 28 0.7903061793097592 2 29 0.9209707880283808 2 30 0.8604420977554987 2 31 0.9514767587024657 2 32 0.9197414906324504 2 33 0.8444340590441151 2 34 0.8750493625213629 2 35 0.8698580693765621 2 36 0.9540905338757426 3 1 0.5650755891266783 3 2 0.6493220245374935 3 3 0.6974143843476881 3 4 0.5483013736980098 3 5 0.6816684852396016 3 6 0.6242649817592181 3 7 0.8304165520591695 3 8 0.7329882336818869 3 9 0.892482861796374 3 10 0.8192354439909764 3 11 0.6563604623178989 3 12 0.506010722033821 3 13 0.5994210640761253 3 14 0.6622137205176308 3 15 0.7195997329807859 3 16 0.646830015854647 3 17 0.6243144855865381 3 18 0.6419301747440258 3 19 0.7122615490693072 3 20 0.7146769016254666 3 21 0.7936798721629899 3 22 0.5146626717885425 3 23 0.6680449677230236 3 24 0.8352002026906717 3 25 0.8015208167628245 3 26 0.8023632438616488 3 27 0.7844346690318424 3 28 0.8508846746964558 3 29 0.6765203032890206 3 30 0.8713215807787016 3 31 0.8142771408993923 3 32 0.8607079486808438 3 33 0.7427549558622353 3 34 0.7519796408311947 3 35 0.7998288903000272 3 36 0.6888425869082653 4 1 0.6315049868949423 4 2 0.7623343045918105 4 3 0.8496945026571865 4 4 0.7830093375374325 4 5 0.752516230468423 4 6 0.773048137744329 4 7 1.0518147394442288 4 8 0.9014152092792429 4 9 1.0460344656128946 4 10 0.8122989153525211 4 11 0.7400050025277736 4 12 0.7240256253106239 4 13 0.7849548805873212 4 14 0.7345702996079915 4 15 0.7341083714188947 4 16 0.8327190729485139 4 17 0.8121697615914272 4 18 0.8279434135538679 4 19 0.871534238593501 4 20 0.8203541569574284 4 21 0.8740738231361824 4 22 0.8929642042803856 4 23 0.7471662421356697 4 24 1.0105752501348633 4 25 0.8960570803593129 4 26 1.0043827002641414 4 27 0.8704800878063139 4 28 1.0025770865211499 4 29 0.9715228603217199 4 30 0.9193368070115184 4 31 0.8760023643727415 4 32 0.7622335237416848 4 33 0.9130206981840239 4 34 0.790144915081008 4 35 0.6757995661144314 4 36 0.9240004923038114 5 1 0.7800808620365132 5 2 0.8632025355756809 5 3 0.9601965391905125 5 4 0.876773075725179 5 5 0.8679709606649908 5 6 0.8435766049121192 5 7 1.0417302564605784 5 8 0.9527153418595695 5 9 0.984560078606691 5 10 0.927426993794537 5 11 0.7851257997884957 5 12 0.7952960706389618 5 13 0.7692589493972051 5 14 0.860126251292904 5 15 0.7949917262965407 5 16 0.858344753709531 5 17 0.812231702971232 5 18 1.0044209556561974 5 19 0.9686803430537552 5 20 1.10604391766363 5 21 0.8425585541287965 5 22 0.9894884901926666 5 23 0.9449693907557553 5 24 0.9593023277615522 5 25 1.0158016547761939 5 26 1.0389605383304037 5 27 0.9155087867582772 5 28 1.0162233148657691 5 29 0.8658298980784096 5 30 0.8302340996430537 5 31 1.000982513744276 5 32 0.8795984539891002 5 33 0.9310131727732767 5 34 0.8398359720815058 5 35 0.9001618816768584 5 36 0.8333003107683972 6 1 0.8603271989088874 6 2 0.8692986628709107 6 3 0.9462098610236787 6 4 0.8080964693489001 6 5 0.8319222531194477 6 6 0.8575820784084272 6 7 1.0682551688513655 6 8 0.7012637245626756 6 9 1.0340977512000222 6 10 0.7341506412787453 6 11 0.7170087644949952 6 12 0.6693204825553458 6 13 0.6112302907971383 6 14 0.6764752836913246 6 15 0.6637070753351573 6 16 0.7101291061096227 6 17 0.4257759210980261 6 18 0.581548230694807 6 19 0.6303676878902884 6 20 0.6848541286574488 6 21 0.7369689447248255 6 22 0.7052438380750701 6 23 0.8414284110656958 6 24 0.8034481453318811 6 25 0.6964227326893351 6 26 0.8323219435879778 6 27 0.7519806991269249 6 28 0.670763586753452 6 29 0.5239278221268435 6 30 0.614707260992926 6 31 0.5845235819520624 6 32 0.6826909073025078 6 33 0.7725769638016438 6 34 0.6828366364279432 6 35 0.6135644776407005 6 36 0.6840966338072647 7 1 0.9525881295253598 7 2 1.0033850616786566 7 3 1.0086705041351998 7 4 0.9000194341920569 7 5 0.9988292660201239 7 6 0.9737818659858419 7 7 1.1493924266356466 7 8 1.0217219371303052 7 9 1.1156907432018848 7 10 0.9693662923825634 7 11 0.8085925856984204 7 12 0.5805062355649078 7 13 0.5714347819061629 7 14 0.7085558841836388 7 15 0.6078896980704229 7 16 0.797827404904421 7 17 0.8445729434960612 7 18 0.8940893041333418 7 19 0.92514563665409 7 20 1.0581327010841477 7 21 1.0482643392312383 7 22 1.099177654644535 7 23 1.1043265251789798 7 24 1.2276058131473775 7 25 1.1125947060762584 7 26 1.0898158932628945 7 27 1.1128020272980628 7 28 1.1048785625525426 7 29 1.1296722197313755 7 30 1.0719281213466232 7 31 1.2251316285904892 7 32 1.2419541666816514 7 33 0.9313932734975552 7 34 1.0100554324880306 7 35 1.0137069232135403 7 36 1.1444677839155233 8 1 0.8167097586878143 8 2 0.8476654666076051 8 3 0.907528115479753 8 4 0.8952471358759269 8 5 0.9163838052192048 8 6 0.8819348660817319 8 7 1.220523291203076 8 8 1.0726447478106458 8 9 1.0955251206975696 8 10 0.9984098064527771 8 11 0.6665973426346277 8 12 1.0082802674683649 8 13 0.9681571824677455 8 14 0.9617302734383237 8 15 0.9162432515733308 8 16 1.048083682731832 8 17 1.0870487456281808 8 18 1.0357583599130216 8 19 1.1107421016306844 8 20 1.09129949121224 8 21 1.144671821620534 8 22 0.9724436280044557 8 23 1.2163232922480276 8 24 1.022099776941257 8 25 1.1821096585909139 8 26 1.3005894316829192 8 27 1.3104200246381197 8 28 1.2433435774360468 8 29 1.2425897384434919 8 30 1.1351738011093957 8 31 1.2820181251431713 8 32 0.9964709495909894 8 33 1.0470356484010104 8 34 0.9625987163690795 8 35 0.9201758899772656 8 36 1.0179033858319748 9 1 0.868811979464656 9 2 0.9644886830966577 9 3 1.0781573765245902 9 4 0.9622957304462716 9 5 1.0569578685897696 9 6 0.8689225586652647 9 7 1.0421421990361246 9 8 0.9503807004948754 9 9 1.1498073210379793 9 10 0.9735509666683768 9 11 0.9101932849935679 9 12 0.5685452566150566 9 13 1.012423689125331 9 14 1.014192640616752 9 15 1.015771928813166 9 16 1.1383994186353874 9 17 0.9781458988675986 9 18 0.9906975954427608 9 19 1.0880206889598902 9 20 1.2701760292718873 9 21 1.15449365685555 9 22 1.2195720493585291 9 23 1.1200790855264273 9 24 1.3125294605293192 9 25 1.1847058787122933 9 26 0.790649042483782 9 27 0.9875893251491554 9 28 0.9286428900842303 9 29 0.9179200164599699 9 30 0.8267755120252502 9 31 0.9316214119357227 9 32 1.2509649632120592 9 33 1.088609417213224 9 34 1.087307379680901 9 35 1.0669988860199382 9 36 0.8692781419500442 10 1 1.069572962931904 10 2 0.9497135666851371 10 3 1.2661425061955924 10 4 1.1239430894884563 10 5 1.1428576506131876 10 6 1.0974022449842593 10 7 1.4737152780277682 10 8 1.1135000116191982 10 9 1.3788850339552674 10 10 1.1711324441641544 10 11 1.216360186323623 10 12 1.2455901450737272 10 13 1.2688399506713424 10 14 1.2358567213685951 10 15 1.2566884969052907 10 16 1.348891713667026 10 17 1.350016157041395 10 18 1.2217492568363824 10 19 1.2382909056435096 10 20 1.3250231525578577 10 21 1.2741872701310335 10 22 1.222325272689197 10 23 1.2476517445873796 10 24 1.3715572238603535 10 25 1.1310691772930177 10 26 1.2125015705928135 10 27 1.2943353929850474 10 28 1.3235561927022133 10 29 1.2014179068140933 10 30 1.326037186066614 10 31 1.2756173124306969 10 32 0.9624078707771311 10 33 1.3316274351618773 10 34 1.2011152231918842 10 35 1.3519719322755854 10 36 1.3156872090778684 11 1 1.3455349444807219 11 2 1.3592707290746056 11 3 1.443315682212134 11 4 1.3656933292938405 11 5 1.4292375978795313 11 6 1.317468990856959 11 7 1.584092781895432 11 8 1.4001645588366216 11 9 1.400600051085604 11 10 1.3192523133991756 11 11 1.0277324660899332 11 12 1.0594636562611441 11 13 1.1077804697437195 11 14 1.072738643784664 11 15 0.9663487183008144 11 16 1.170054808421213 11 17 1.0723151519851584 11 18 1.224010102849121 11 19 1.1403424389321053 11 20 1.2055294833136578 11 21 1.1147551987068596 11 22 1.212415532517511 11 23 1.1653457467804635 11 24 1.3337489134519025 11 25 1.1586705377899165 11 26 0.9634669166988488 11 27 1.1155210567380252 11 28 1.095440565038917 11 29 1.1023121132967972 11 30 0.939869887175881 11 31 1.3363119290389882 11 32 1.078757367387233 11 33 1.0844313841321274 11 34 1.242649933051716 11 35 1.2876791005850627 11 36 1.2674554015677444 12 1 1.2861673385797125 12 2 1.3482198593802721 12 3 1.377700851556181 12 4 1.26318014667849 12 5 1.402106412910367 12 6 1.2257554261959847 12 7 1.6674421738539849 12 8 1.3433047536781366 12 9 1.5048346687447598 12 10 1.1907756521690658 12 11 1.2592245632311432 12 12 1.1456767239535353 12 13 1.2476672505754358 12 14 1.0759352966604006 12 15 1.252457663795635 12 16 1.2195632609517695 12 17 1.0928749172073964 12 18 1.1755854438731141 12 19 1.1833048851156072 12 20 1.2367313746964115 12 21 1.0174746814778821 12 22 1.1558560666046525 12 23 1.1627949537727176 12 24 1.1713183177973825 12 25 1.1977179555184279 12 26 1.1380289232079974 12 27 1.2648364591066255 12 28 1.2313996985372064 12 29 1.1912410021606163 12 30 1.0949410489109574 12 31 1.3066658619156861 12 32 1.0932571261548567 12 33 1.0805487511003946 12 34 1.1052282374421232 12 35 1.1426013494443978 12 36 1.229448110830802 13 1 1.3401436370543856 13 2 1.468403268403403 13 3 1.5796267834187603 13 4 1.4964123659363011 13 5 1.5079569139465718 13 6 1.487052130975595 13 7 1.7255895028021868 13 8 1.3489519771708238 13 9 1.5874048022351914 13 10 1.422580640878305 13 11 1.197130439603196 13 12 1.2386119347171132 13 13 1.34157126328979 13 14 1.2411914235911716 13 15 1.3266638553530115 13 16 1.3621051622397395 13 17 1.303217126397925 13 18 1.306140565881698 13 19 1.253618639421834 13 20 1.3417817199971296 13 21 1.3137329272108644 13 22 1.2784520991287849 13 23 1.4469719783457506 13 24 1.4808031248109046 13 25 1.3941201542190207 13 26 1.369856331538589 13 27 1.3328435599658448 13 28 1.3322926840331064 13 29 1.323877459389056 13 30 1.329310630290852 13 31 1.3208917208570163 13 32 1.31327108049339 13 33 1.1536016469101007 13 34 1.1127721578038816 13 35 1.2129274820386513 13 36 1.1822460933429753 14 1 1.4462800656417052 14 2 1.527559379387725 14 3 1.5758071478147926 14 4 1.5165682055113159 14 5 1.5395702388950205 14 6 1.5219623698288842 14 7 1.7445257352237498 14 8 1.6340722639805492 14 9 1.7155163200434096 14 10 1.4975429249891832 14 11 1.4706367637072333 14 12 1.4241762038199504 14 13 1.4251821926820334 14 14 1.493447123459756 14 15 1.433769702388119 14 16 1.435923613000952 14 17 1.3455228973039972 14 18 1.426162017122941 14 19 1.4479346818856733 14 20 1.5970198023191102 14 21 1.3211949535856888 14 22 1.1776760341144552 14 23 1.427226395947379 14 24 1.3042727807217673 14 25 1.3293339662429133 14 26 1.331422007539281 14 27 1.451166803908201 14 28 1.4169700961842864 14 29 1.3798850375565337 14 30 1.0464702453462564 14 31 1.3114755894214838 14 32 1.3619322995760896 14 33 1.3270475704946172 14 34 1.372555725905773 14 35 1.4024079720655038 14 36 1.375329378405656 15 1 1.1971073938901142 15 2 1.261610928014239 15 3 1.2588765831105597 15 4 1.2247594807169406 15 5 1.2004132753986134 15 6 1.2585022425596328 15 7 1.4619818405615526 15 8 1.3370679471189 15 9 1.3340390323251334 15 10 1.218712532271791 15 11 1.1506237457091153 15 12 1.039017923289471 15 13 1.140963946570384 15 14 1.0797449509450558 15 15 1.1294088105651667 15 16 1.143144782622997 15 17 1.146349001445234 15 18 1.0642158581686587 15 19 1.0925905253053507 15 20 1.1405882825499134 15 21 0.8901010672263503 15 22 1.1390316480634937 15 23 1.0979028615359354 15 24 1.0614287456447435 15 25 0.9928216959696101 15 26 1.0020204539882904 15 27 0.9909112825774571 15 28 1.1006669226581849 15 29 0.9403291693408917 15 30 1.1014387704396285 15 31 1.1847542168261995 15 32 1.0466671597257822 15 33 1.0804355786350541 15 34 1.0563439206002485 15 35 1.0363134018751126 15 36 1.1057917644005464 16 1 1.3596444239059515 16 2 1.3880868240430644 16 3 1.4529193498805348 16 4 1.4370513328415697 16 5 1.3699956468455419 16 6 1.3144896209228598 16 7 1.6516757798731838 16 8 1.2913759832233218 16 9 1.5319672829979716 16 10 1.3220259069965294 16 11 1.0679821974119506 16 12 1.0241299129535193 16 13 1.0225197292704742 16 14 1.1479370339520294 16 15 1.1498756939781458 16 16 1.2653409057597038 16 17 1.0734668423766927 16 18 1.258743666754679 16 19 1.1083016117292281 16 20 1.3363093657097345 16 21 1.2767193233865441 16 22 1.0851095886230269 16 23 1.0802726642872003 16 24 1.129025724507688 16 25 1.0518192516582907 16 26 1.241450894329649 16 27 0.8907048704279497 16 28 1.1671031917553165 16 29 1.2046243994133867 16 30 1.1131385167791592 16 31 1.0924909930640005 16 32 1.1948636320236936 16 33 1.012852283540301 16 34 0.8562078980399797 16 35 0.8857830803107412 16 36 0.9799082920194451 17 1 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0.9933116224700065 110 3 1.0254139994319544 110 4 0.7785262461586413 110 5 0.8765475380276797 110 6 0.8385889391957377 110 7 0.9697220732549283 110 8 0.8151945183150815 110 9 0.8596503440675036 110 10 0.7296883470537594 110 11 0.5440774787859475 110 12 0.6293804416022795 110 13 0.4862732511961525 110 14 0.5306780586902207 110 15 0.42056039097309394 110 16 0.40176674952284586 110 17 0.6332899997913408 110 18 0.5627413481929295 110 19 0.5585455329820341 110 20 0.6795132307615681 110 21 0.4554395290709792 110 22 0.5843524109057618 110 23 0.6442642893104207 110 24 0.6864884601253713 110 25 0.44442056991735157 110 26 0.6944772405183954 110 27 0.5019485090108269 110 28 0.5691941041668152 110 29 0.5646875783375833 110 30 0.5673992560883114 110 31 0.45046329707823674 110 32 0.44519567272346094 110 33 0.4847242367226485 110 34 0.5026872999161185 110 35 0.6840613188708424 110 36 0.5260752119145522 111 1 0.9034699603460119 111 2 0.7274042204544777 111 3 0.8295700010485518 111 4 0.6264491342416889 111 5 0.780479963717351 111 6 0.6185273429760159 111 7 0.8791625635594968 111 8 0.6237242110391676 111 9 0.8144831574323206 111 10 0.6444328026433652 111 11 0.48828255905678475 111 12 0.5544175182480231 111 13 0.45276239309205124 111 14 0.5661252678281805 111 15 0.5510009986113182 111 16 0.5669953545994808 111 17 0.47515222913873956 111 18 0.4485738719766985 111 19 0.5621972467843044 111 20 0.5100321200977547 111 21 0.6299952216694393 111 22 0.46408457214037924 111 23 0.5170905055211925 111 24 0.49712586238188905 111 25 0.5854198734215963 111 26 0.5587867418187464 111 27 0.17546928516705051 111 28 0.5440423802904455 111 29 0.5503112916673558 111 30 0.6168193433734928 111 31 0.4725614322632019 111 32 0.3928250093774288 111 33 0.2950061812060518 111 34 0.3498640624790177 111 35 0.3475731290763413 111 36 0.40297467395750886 112 1 0.7917944664206011 112 2 0.776445436868066 112 3 0.8564464235302829 112 4 0.7082332629071679 112 5 0.6192831929453391 112 6 0.555664116073153 112 7 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0.5534184776341533 113 11 0.4592900098806396 113 12 0.4835693015916682 113 13 0.43523198993452894 113 14 0.47993389511104567 113 15 0.5839265336310504 113 16 0.41104184977015024 113 17 0.4297518431971845 113 18 0.4302063100622391 113 19 0.34686458640386064 113 20 0.3552628975087764 113 21 0.4691675859746418 113 22 0.518887292171506 113 23 0.4363497808533907 113 24 0.5486208659805065 113 25 0.24598873929609155 113 26 0.351737280682503 113 27 0.4289061698716523 113 28 0.40635349097328444 113 29 0.39011627374636504 113 30 0.511297998528832 113 31 0.415330268993813 113 32 0.28487636514342185 113 33 0.3842824797830602 113 34 0.33186053914706193 113 35 0.39615258310962886 113 36 0.60966739288083 114 1 0.6928654639180891 114 2 0.612967763997645 114 3 0.6513654947455727 114 4 0.39930514228445446 114 5 0.42785243498625647 114 6 0.4326033930028288 114 7 0.6632264230478029 114 8 0.4228894220464845 114 9 0.6154533703295257 114 10 0.457395008644621 114 11 0.20050025395699222 114 12 0.3554669671327879 114 13 0.33258707856189673 114 14 0.31806979566405147 114 15 0.2128432577795536 114 16 0.20409288889565858 114 17 0.332367203374087 114 18 0.1609966768018829 114 19 0.2894526503130521 114 20 0.30478659375715367 114 21 0.2849070535337326 114 22 0.3979101645822709 114 23 0.43776315174562086 114 24 0.4240617731038796 114 25 0.3229991149057625 114 26 0.41614298835156305 114 27 0.37334848262179676 114 28 0.36176593982215266 114 29 0.3777852686744403 114 30 0.2607976533110552 114 31 0.39359605694808053 114 32 -0.06630206269531058 114 33 0.22945171429335076 114 34 0.2851176443370136 114 35 0.27013208775584374 114 36 0.25524408441877 115 1 0.5808396768101538 115 2 0.5656986165974409 115 3 0.6177481184341289 115 4 0.5016631959160378 115 5 0.45112264247838496 115 6 0.3037996860855756 115 7 0.5904996935602611 115 8 0.43175752561344183 115 9 0.5794017684077275 115 10 0.3312707897456093 115 11 0.28657418489407505 115 12 0.2413862767220607 115 13 0.29617496560701895 115 14 0.2609740215935936 115 15 0.2828747391825708 115 16 0.14380716748247124 115 17 0.18212846817077732 115 18 -0.21169029575973397 115 19 0.2901391559246278 115 20 0.3282515791872278 115 21 0.28060864329978585 115 22 0.19243347931869792 115 23 0.29342904631823785 115 24 0.3617909960863841 115 25 0.2025831259965436 115 26 0.15376875396266598 115 27 0.3619591116814398 115 28 0.2196938132337003 115 29 0.20112861935544402 115 30 0.2978629915217148 115 31 0.0803733157267068 115 32 0.08764289943024295 115 33 -0.03296789010046197 115 34 0.23054881642775982 115 35 0.0030484453108456933 115 36 8.252712827889752E-4 116 1 0.5366541414635719 116 2 0.4399601840935148 116 3 0.5066856132235849 116 4 0.3770384864424834 116 5 0.3458566059849808 116 6 0.2714574071744451 116 7 0.4429127513729689 116 8 0.26437069401777435 116 9 0.3957705862184291 116 10 0.19552734750291023 116 11 0.1046199226864637 116 12 0.013843752006849286 116 13 0.03399612189471668 116 14 0.1477903876350979 116 15 0.05705427295245613 116 16 0.08011715366765415 116 17 0.13639163226240214 116 18 0.2070398585982396 116 19 0.2564165595637107 116 20 0.21505294231751293 116 21 0.23436149772501835 116 22 0.13084647610297395 116 23 0.17599465876115872 116 24 0.19896768993357128 116 25 0.03150959347068749 116 26 0.10468260629226833 116 27 0.1576638513291826 116 28 0.09699682441782898 116 29 -0.021801559769013323 116 30 -0.011361323022150316 116 31 0.11767349191480747 116 32 0.12059551912059874 116 33 -0.13788023452680065 116 34 -0.07931913552626568 116 35 -0.18168502032260747 116 36 -0.0776615862364487 117 1 0.5085850347693217 117 2 0.4176758040424815 117 3 0.5095402195218846 117 4 0.3425660224763405 117 5 0.3596855828618608 117 6 0.3058166389015078 117 7 0.3338041031570249 117 8 0.2541505140431397 117 9 0.3712896019881633 117 10 0.1631045204331194 117 11 0.12457849788070519 117 12 0.12455459526000112 117 13 0.09974844790119519 117 14 0.0415265020981046 117 15 0.10676994513787008 117 16 0.12724331026977964 117 17 0.11667854567364344 117 18 0.05627208138014339 117 19 0.10536248700195827 117 20 0.3108914910814491 117 21 -0.01176538679416339 117 22 -0.22400277186123319 117 23 0.05917887029707458 117 24 0.21240447975100807 117 25 0.08302574144507702 117 26 0.11528195901911431 117 27 0.1462300371732006 117 28 0.025891083891441014 117 29 0.017109308672811352 117 30 0.15685069779132732 117 31 0.05073394566291611 117 32 -0.18055703473811135 117 33 0.03371791815964187 117 34 0.09052161822035387 117 35 0.0034678395112166527 117 36 0.23423166761384806 118 1 0.36583940632585765 118 2 0.276145165412089 118 3 0.3716602937097804 118 4 0.2909424243028589 118 5 0.23719282487378734 118 6 0.09748944708816865 118 7 0.4056382609764846 118 8 0.169513913441957 118 9 0.2161644446898876 118 10 0.043567144681553316 118 11 -0.05324077708359454 118 12 -0.04854688324184053 118 13 -0.05137948305274165 118 14 -2.0100976994186226E-4 118 15 -0.0019796186992309117 118 16 0.03091515510444154 118 17 -0.053105316137546765 118 18 -0.03788594142626067 118 19 -0.0210074865234553 118 20 0.08324130166747068 118 21 0.04858276971045683 118 22 -0.4832611415686152 118 23 -0.09924749802999955 118 24 -0.09687561491835339 118 25 0.021875716111249943 118 26 -0.003921018801648302 118 27 -0.22311019409493937 118 28 -0.03976267807053446 118 29 -0.16057178834321562 118 30 0.03868661553099173 118 31 0.021424334952900637 118 32 0.009952693367779375 118 33 -0.10566311026650865 118 34 -0.1747967614416872 118 35 -0.1160830551352845 118 36 -0.060023584885396275 119 1 0.4986294420845745 119 2 0.43902022964651266 119 3 0.503686712297224 119 4 0.4743279035630117 119 5 0.5118420578632517 119 6 0.36179006145865245 119 7 0.5769557665562566 119 8 0.4053595582390095 119 9 0.42870743237006437 119 10 0.33918396578127386 119 11 0.3043097200517053 119 12 0.1914787005538443 119 13 0.2123453400763644 119 14 0.138032483038792 119 15 0.24263044884299134 119 16 0.10155213863197332 119 17 0.1813151345145485 119 18 0.16073335297706492 119 19 0.1587467837468104 119 20 0.3558522255972605 119 21 0.0941816793613981 119 22 0.23446874583678934 119 23 0.3081807039739175 119 24 0.38163965563403934 119 25 0.4228033174097199 119 26 0.2529410860713952 119 27 0.21579974607115204 119 28 0.303844221305944 119 29 0.24677106073086463 119 30 0.10118792086248973 119 31 0.22223890759802245 119 32 0.14409324047337435 119 33 0.34894114021855316 119 34 0.16966868803722335 119 35 0.19717594956927087 119 36 0.1818849525649533 120 1 -24.497443642180492 120 2 -23.508948136865623 120 3 -23.207030495229365 120 4 -23.03216241549889 120 5 -22.799911419093387 120 6 -22.230192485582524 120 7 -21.733543278985056 120 8 -21.705821243506286 120 9 -21.443722346740927 120 10 -21.439708862143462 120 11 -21.386745638552057 120 12 -21.23328268477476 120 13 -21.13355048757567 120 14 -21.016098959557336 120 15 -20.886766878672688 120 16 -20.748761469221858 120 17 -20.579579329625076 120 18 -20.434603434587704 120 19 -20.288158043087115 120 20 -20.03787561732611 120 21 -19.908381723965572 120 22 -19.724980395150393 120 23 -19.58301100587064 120 24 -19.33859486850013 120 25 -19.25881653533094 120 26 -18.96887511245601 120 27 -18.744167529631056 120 28 -18.672430662795502 120 29 -18.51338710670846 120 30 -18.27721404259538 120 31 -18.18359692550806 120 32 -17.946307632174566 120 33 -17.727551905868165 120 34 -17.508978707654077 120 35 -17.271054211325193 120 36 -17.177755080159343 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 1.0120483E7 3 513164.0 4 524427.0 5 597776.0 6 549622.0 7 238738.0 8 284017.0 9 317588.0 10 243055.0 11 187175.0 12 205390.0 13 229762.0 14 221593.0 15 197113.0 16 203979.0 17 218569.0 18 227890.0 19 238136.0 20 272754.0 21 315520.0 22 356108.0 23 392724.0 24 434670.0 25 488358.0 26 542015.0 27 586979.0 28 622269.0 29 687065.0 30 800570.0 31 957764.0 32 1176694.0 33 1480590.0 34 1910422.0 35 2456465.0 36 3062086.0 37 3444250.0 38 2755967.0 39 1400339.0 40 201.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 19.598045740872504 29.79886628756651 23.246117112026464 27.356970859534517 2 25.384483772430915 29.622527724523025 24.086251841986655 20.906736661059405 3 24.11606302695782 27.63087852790096 24.676312481145857 23.576745963995364 4 26.89103286816834 26.418595854569393 23.39255958510419 23.297811692158078 5 26.87615580536918 27.695511527403 24.263728328749398 21.164604338478426 6 25.863106831180644 26.523330631777903 22.17402657173723 25.439535965304223 7 24.61827657271895 26.067616387809846 25.195948616919274 24.11815842255193 8 24.532183068313298 25.670067359016212 25.097134707545816 24.700614865124667 9 23.89463618637767 25.381116495298144 25.39305118287013 25.331196135454054 10 24.150798138000606 26.73472799476746 23.711353519248178 25.403120347983755 11 24.401689428362218 26.087973965749946 24.169539374166455 25.340797231721385 12 24.315086604035532 25.675012141411635 24.808964734921233 25.2009365196316 13 24.139843454867616 25.889237228603807 24.92033648351961 25.050582833008967 14 24.06835376073714 25.98996900914981 25.032990136824335 24.908687093288712 15 23.72654638818853 25.64291533927981 25.208949517828167 25.42158875470349 16 23.85847456917344 25.61835221218866 25.0500228737435 25.473150344894403 17 23.92305001004137 25.477164668297732 25.065042395022246 25.534742926638653 18 23.71686031925158 25.361624049592667 24.984298649642785 25.937216981512968 19 23.9783283286043 25.41195485667463 24.857375436043093 25.75234137867798 20 23.927869611434392 25.447182318490324 24.925432461622403 25.699515608452884 21 23.83554228122822 25.248304115824975 24.84236767846383 26.073785924482966 22 23.910590531413025 25.268943273014045 24.712237562870023 26.108228632702907 23 23.95248019292853 25.34643172335468 24.831434991753905 25.869653091962885 24 23.959354276061635 25.16982731706305 24.579311556158654 26.29150685071666 25 24.121871893530315 25.19614746472863 24.48162498585724 26.20035565588381 26 24.206956283991985 25.190158211643453 24.562047283199806 26.04083822116475 27 24.20026017307764 24.94941608388635 24.466087579581195 26.384236163454815 28 24.136655708489023 25.17118248478145 24.522546938421737 26.16961486830779 29 24.105387588142474 25.20035671520176 24.647199178294247 26.047056518361515 30 23.962476445968452 25.050441976125086 24.549444349250766 26.437637228655692 31 24.03112250929261 25.073556500063315 24.429434972926604 26.465886017717473 32 24.038831798035513 25.068429624760856 24.54589887981588 26.346839697387747 33 23.977900744344264 24.879085543487562 24.455948278517088 26.68706543365108 34 24.06258706008576 24.86694047256236 24.306740732653875 26.763731734698005 35 24.038331747927934 24.92779535022297 24.42173167207865 26.612141229770447 36 24.01709725837383 24.788979134313244 24.323215030629843 26.870708576683082 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1177516.0 1 1227663.5 2 1277811.0 3 1277811.0 4 1009436.0 5 741061.0 6 741061.0 7 580377.5 8 419694.0 9 419694.0 10 334591.5 11 249489.0 12 249489.0 13 208726.5 14 167964.0 15 160344.5 16 152725.0 17 152725.0 18 174149.0 19 195573.0 20 195573.0 21 245726.0 22 295879.0 23 295879.0 24 367938.0 25 439997.0 26 548907.5 27 657818.0 28 657818.0 29 814490.5 30 971163.0 31 971163.0 32 1149475.5 33 1327788.0 34 1327788.0 35 1522079.0 36 1716370.0 37 1716370.0 38 1910036.5 39 2103703.0 40 2308611.5 41 2513520.0 42 2513520.0 43 2709234.5 44 2904949.0 45 2904949.0 46 3043615.0 47 3182281.0 48 3182281.0 49 3244217.5 50 3306154.0 51 3286653.0 52 3267152.0 53 3267152.0 54 3144267.0 55 3021382.0 56 3021382.0 57 2835415.0 58 2649448.0 59 2649448.0 60 2397602.5 61 2145757.0 62 2145757.0 63 1880933.5 64 1616110.0 65 1375142.0 66 1134174.0 67 1134174.0 68 937140.0 69 740106.0 70 740106.0 71 600703.0 72 461300.0 73 461300.0 74 369277.0 75 277254.0 76 218955.0 77 160656.0 78 160656.0 79 125810.0 80 90964.0 81 90964.0 82 70476.0 83 49988.0 84 49988.0 85 37592.5 86 25197.0 87 25197.0 88 18595.5 89 11994.0 90 8551.5 91 5109.0 92 5109.0 93 3601.0 94 2093.0 95 2093.0 96 1614.5 97 1136.0 98 1136.0 99 1074.0 100 1012.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 3.3842716718369616 2 4.026064176158873 3 6.6146951898657065 4 6.610607743033241 5 8.994810665686963 6 8.435349932962579 7 10.369507981126386 8 9.38215263600916 9 11.140849490046028 10 9.929883181884517 11 10.045352921385422 12 10.154464691820825 13 10.220570845273109 14 10.319128235015878 15 10.39097404567556 16 10.441500767555615 17 10.499644888802314 18 10.560847626494633 19 11.301235531534196 20 10.761061567465667 21 10.77009044103298 22 10.670914739432106 23 11.41935083488699 24 10.838910578091939 25 10.814358022382232 26 10.819489503991495 27 10.905054742519106 28 10.886748657015241 29 10.840517175297013 30 10.95577151927358 31 10.867705158598637 32 10.902667653296424 33 10.973578393973973 34 10.914139365516245 35 10.864213217039346 36 10.927116312341452 >>END_MODULE >>Sequence Length Distribution pass #Length Count 36 3.9462287E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.65765510048116 #Duplication Level Percentage of deduplicated Percentage of total 1 96.33697801580523 85.41010570347896 2 2.30364718933779 4.084719159710053 3 0.6172158454405748 1.6416272864286712 4 0.2864180769264674 1.0157262031475933 5 0.1433913865701855 0.6356372047459636 6 0.07270728997978924 0.3867634702991295 7 0.04549657718745286 0.2823533893976331 8 0.03473921026401353 0.24639175376400058 9 0.025375383358355742 0.20247497872448342 >10 0.11760689680034321 1.8868090637884953 >50 0.008490644219994117 0.5280316824122105 >100 0.007345210481728052 1.1632992553070958 >500 1.8687909856281252E-4 0.1106165626244663 >1k 2.7004389127261363E-4 0.503870949148175 >5k 8.104614384610596E-5 0.5400120693070942 >10k+ 5.0304494422104113E-5 1.361561267716091 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 324261 0.8216984484452206 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.547690989120828E-5 3.0408779906749954E-5 0.0 0.0 2.534064992229163E-6 2 4.054503987566661E-5 5.3215364836812424E-5 0.0 1.2670324961145816E-5 2.534064992229163E-6 3 5.5749429829041586E-5 1.2163511962699982E-4 0.0 1.2670324961145816E-5 1.2670324961145816E-5 4 6.081755981349991E-5 1.4444170455706228E-4 0.0 1.5204389953374977E-5 1.2670324961145816E-5 5 6.081755981349991E-5 1.6724828948712475E-4 0.0 1.5204389953374977E-5 1.773845494560414E-5 6 6.335162480572907E-5 1.9258893940941638E-4 0.0 1.5204389953374977E-5 2.0272519937833305E-5 7 6.335162480572907E-5 2.1032739435502052E-4 2.534064992229163E-6 1.773845494560414E-5 2.534064992229163E-5 8 6.84197547901874E-5 2.2806584930062468E-4 2.534064992229163E-6 1.773845494560414E-5 3.0408779906749954E-5 9 1.0643072967362485E-4 2.534064992229163E-4 2.534064992229163E-6 2.534064992229163E-5 3.0408779906749954E-5 10 7.62753562660978E-4 2.736790191607496E-4 2.534064992229163E-6 2.7874714914520793E-5 3.294284489897912E-5 11 8.919908772646654E-4 2.939515390985829E-4 2.534064992229163E-6 3.801097488343744E-5 3.547690989120828E-5 12 9.553425020703945E-4 3.091559290519579E-4 2.534064992229163E-6 4.8147234852354095E-5 4.8147234852354095E-5 13 9.857512819771443E-4 3.344965789742495E-4 2.534064992229163E-6 5.068129984458326E-5 6.335162480572907E-5 14 0.0010338985168294986 3.5983722889654116E-4 5.068129984458326E-6 5.828349482127075E-5 7.855601475910405E-5 15 0.0025315309272369337 3.699734888654578E-4 5.068129984458326E-6 6.588568979795824E-5 9.122633972024987E-5 16 0.0026176891369727256 3.826438138266036E-4 7.602194976687489E-6 6.588568979795824E-5 9.882853469693736E-5 17 0.002772267101498704 4.0798446374889526E-4 7.602194976687489E-6 6.588568979795824E-5 1.0389666468139568E-4 18 0.0029749923008770373 4.257229186944994E-4 7.602194976687489E-6 6.84197547901874E-5 1.0389666468139568E-4 19 0.003312022944843516 4.4852950362456184E-4 7.602194976687489E-6 7.602194976687488E-5 1.1910105463477066E-4 20 0.035575738425905216 4.8654047850799927E-4 7.602194976687489E-6 7.602194976687488E-5 1.3177137959591648E-4 21 0.0362168568689392 5.220173883992076E-4 7.602194976687489E-6 7.602194976687488E-5 1.368395095803748E-4 22 0.03642465019830199 5.473580383214993E-4 7.602194976687489E-6 7.602194976687488E-5 1.4444170455706228E-4 23 0.03668565889250159 5.676305582593325E-4 7.602194976687489E-6 7.602194976687488E-5 1.5204389953374977E-4 24 0.03692892913175559 5.777668182282491E-4 1.0136259968916653E-5 7.602194976687488E-5 1.6218015950266644E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGGA 4265 0.0 11.378109 21 AGATCGG 4225 0.0 11.284529 20 CGGTTCA 4830 0.0 11.057933 1 CGAGATC 4575 0.0 10.419094 18 GAGATCG 4745 0.0 10.078857 19 AATGCCG 5400 0.0 9.7895565 13 AGCGGTT 5020 0.0 8.981962 30 GAGCGGT 5475 0.0 8.316535 29 ATGCCGA 6525 0.0 8.025233 14 ATCGGAA 6160 0.0 8.024151 22 CCGAGAT 6310 0.0 7.9277396 17 TGCCGAG 6890 0.0 7.4517827 15 GCCGAGA 7040 0.0 7.3153186 16 TCGGAAG 7060 0.0 7.065305 23 AAGAGCG 6605 0.0 6.983647 27 AGTGGTA 12640 0.0 6.8572702 2 TCAACGC 13325 0.0 6.818999 9 GGTATCA 13620 0.0 6.5884814 5 CAACGCA 13625 0.0 6.578308 10 ATCAACG 14180 0.0 6.476303 8 >>END_MODULE