FastQCFastQC Report
Fri 27 May 2016
SRR522054_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522054_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53767063
Sequences flagged as poor quality0
Sequence length36
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4044350.7521984230382828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG52800.09.66983720
GATCGGA53000.09.44766821
GAGATCG57700.08.78861519
CGGTTCA62050.08.7044251
CGAGATC58150.08.69245618
AATGCCG72800.08.06858213
CCGAGAT72050.07.3475717
GAGCGGT67950.07.11515929
AGCGGTT65000.07.085801630
ATCGGAA73100.07.042032722
ATGCCGA81250.06.854130714
GCCGAGA84250.06.82494116
TGCCGAG83250.06.73494715
TCAACGC158750.06.59381449
GGTATCA164450.06.32685475
AGTGGTA155350.06.1827412
ATCAACG173800.06.15938578
GTATCAA167800.06.14451936
CAACGCA167400.05.965158510
TCGGAAG92200.05.94685423
Produced by FastQC (version 0.11.5)