##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR522054_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 53767063 Sequences flagged as poor quality 0 Sequence length 36 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.008167323552712 29.0 2.0 35.0 2.0 39.0 2 19.846541999141742 25.0 2.0 35.0 2.0 39.0 3 19.750793678278466 24.0 2.0 35.0 2.0 39.0 4 19.454920831364735 24.0 2.0 35.0 2.0 39.0 5 19.289790349902503 23.0 2.0 35.0 2.0 39.0 6 18.629494008255573 20.0 2.0 35.0 2.0 39.0 7 18.26686897515678 17.0 2.0 35.0 2.0 39.0 8 18.1364921866757 17.0 2.0 35.0 2.0 39.0 9 17.86968094202951 15.0 2.0 35.0 2.0 39.0 10 17.73335346585697 14.0 2.0 35.0 2.0 39.0 11 17.64117573243679 11.0 2.0 35.0 2.0 39.0 12 17.499047083899672 10.0 2.0 35.0 2.0 39.0 13 17.370084953310542 9.0 2.0 35.0 2.0 38.0 14 17.25038416176833 7.0 2.0 35.0 2.0 38.0 15 17.049880258477202 2.0 2.0 35.0 2.0 38.0 16 16.961593122540467 2.0 2.0 35.0 2.0 38.0 17 16.846312788184097 2.0 2.0 35.0 2.0 38.0 18 16.73037307245144 2.0 2.0 34.0 2.0 38.0 19 16.584369058804644 2.0 2.0 34.0 2.0 38.0 20 16.406474536278836 2.0 2.0 34.0 2.0 38.0 21 16.307979905839378 2.0 2.0 34.0 2.0 38.0 22 16.138990556356035 2.0 2.0 33.0 2.0 38.0 23 16.013651387281467 2.0 2.0 33.0 2.0 38.0 24 15.868716485406688 2.0 2.0 33.0 2.0 38.0 25 15.722156462219258 2.0 2.0 33.0 2.0 38.0 26 15.500935358883188 2.0 2.0 33.0 2.0 38.0 27 15.298409660204054 2.0 2.0 33.0 2.0 38.0 28 15.205217253544237 2.0 2.0 33.0 2.0 38.0 29 15.046557424942478 2.0 2.0 33.0 2.0 38.0 30 14.896286319377348 2.0 2.0 33.0 2.0 38.0 31 14.821645270079193 2.0 2.0 33.0 2.0 38.0 32 14.582711222296073 2.0 2.0 33.0 2.0 38.0 33 14.35462591661367 2.0 2.0 32.0 2.0 38.0 34 14.124216418516294 2.0 2.0 32.0 2.0 38.0 35 13.976304582602921 2.0 2.0 32.0 2.0 37.0 36 13.942584143009634 2.0 2.0 33.0 2.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -18.66501798704589 1 2 -17.503552997245706 1 3 -17.417017320803335 1 4 -17.118145618061753 1 5 -16.96330774606627 1 6 -16.31595040152594 1 7 -15.952923273363044 1 8 -15.835508901448232 1 9 -15.559290847096353 1 10 -15.433308378977152 1 11 -15.342464707845384 1 12 -15.204776168985923 1 13 -15.079123660582532 1 14 -14.960815869916726 1 15 -14.75725525793031 1 16 -14.673673860748274 1 17 -14.564592823475746 1 18 -14.452877058108715 1 19 -14.308166287381052 1 20 -14.129928111676982 1 21 -14.038555047337319 1 22 -13.869108334424558 1 23 -13.749551484572805 1 24 -13.605663448609882 1 25 -13.46096372754082 1 26 -13.24320997390335 1 27 -13.045198597271819 1 28 -12.94927681953995 1 29 -12.794018972071326 1 30 -12.654569595119627 1 31 -12.577288692326027 1 32 -12.337249728541666 1 33 -12.113682167321763 1 34 -11.891571258346698 1 35 -11.744180927827887 1 36 -11.711002210308802 2 1 -0.289177226998806 2 2 -0.13977714387425877 2 3 -0.13761173815662175 2 4 -0.055193709536446534 2 5 -0.058776049412497144 2 6 0.11739834574159147 2 7 0.16114684602488083 2 8 0.11276333665501426 2 9 0.1959218651955581 2 10 0.21054566507867278 2 11 0.2264565970142094 2 12 0.17070726804114855 2 13 0.17346167656224765 2 14 0.12611871895161286 2 15 0.04861324719832538 2 16 0.1853306793559817 2 17 0.17500277094160666 2 18 0.21575838833486927 2 19 0.1439944659399508 2 20 0.12120691438657971 2 21 0.2016080601118162 2 22 0.19563018920080033 2 23 0.25412684063318025 2 24 0.2694206273948261 2 25 0.2005252585025623 2 26 0.27495044311363337 2 27 0.274143087611316 2 28 0.2685851276603195 2 29 0.3172135821576969 2 30 0.271128235719452 2 31 0.13304845245285257 2 32 0.2588911828422251 2 33 0.3507116446842904 2 34 0.35176571828017345 2 35 0.2545369131003117 2 36 0.29282324790542447 3 1 0.134337082821542 3 2 0.33858665331175786 3 3 0.2451109486341423 3 4 0.3427054367994593 3 5 0.2926199205933706 3 6 0.19280673801923953 3 7 0.3337544934082999 3 8 0.2638862131006334 3 9 0.3859954723758996 3 10 0.36208437504312485 3 11 0.29135829466267893 3 12 0.34961083124591497 3 13 0.1317642775378971 3 14 0.3684146399152972 3 15 0.3536474334938049 3 16 0.28822046344503605 3 17 0.13087791229258627 3 18 0.26662048652002923 3 19 0.18396177129494262 3 20 0.4795308636006972 3 21 0.3499837433133024 3 22 0.2843049477639905 3 23 0.2952774824851758 3 24 0.19432390570124447 3 25 0.4365851164324557 3 26 0.2797524894768433 3 27 0.26936291346647856 3 28 0.3795110911814863 3 29 0.2574279054973303 3 30 0.3969826631896449 3 31 0.31507541920894866 3 32 0.23355559107812063 3 33 0.1546171982861786 3 34 0.20150101176521673 3 35 0.23097029353489695 3 36 0.223514197472511 4 1 0.45233748989608813 4 2 0.570397899867011 4 3 0.47967172954936643 4 4 0.6420806195724964 4 5 0.5594537088027103 4 6 0.47463150369998175 4 7 0.41602624683523004 4 8 0.48904042386369184 4 9 0.3666311559964086 4 10 0.5532190428035157 4 11 0.5703024665143523 4 12 0.5413843580545574 4 13 0.581964737324558 4 14 0.5902676558315747 4 15 0.668740886709255 4 16 0.573408139802492 4 17 0.5341001669705854 4 18 0.7551641222247092 4 19 0.75902713732291 4 20 0.7258983547368985 4 21 0.6300322384465709 4 22 0.6707056762094599 4 23 0.7520690378073489 4 24 0.6580474417241788 4 25 0.7397903979066758 4 26 0.7198887190870966 4 27 0.6004683588999349 4 28 0.6028446878636124 4 29 0.6712933756737112 4 30 0.5343218828391496 4 31 0.5726890652099321 4 32 0.6139015837637132 4 33 0.5152499825712624 4 34 0.467633944271558 4 35 0.5598841472669083 4 36 0.5787429546031806 5 1 0.30244511160548626 5 2 0.4071829059081651 5 3 0.3029571933731532 5 4 0.4094746431679397 5 5 0.44542114848613323 5 6 0.5119140988457289 5 7 0.5562834803801877 5 8 0.4374442683510651 5 9 0.5605774761488433 5 10 0.49870818680813045 5 11 0.593693304260384 5 12 0.49113548015924025 5 13 0.5259097895608242 5 14 0.522437820218137 5 15 0.6236303744366865 5 16 0.508082537255337 5 17 0.5855823915608909 5 18 0.4924346122746357 5 19 0.50941893084131 5 20 0.3618420911476967 5 21 0.5821946606376187 5 22 0.5633436141790309 5 23 0.5177098513091103 5 24 0.4367067329766101 5 25 0.5639274267546046 5 26 0.6906543140864567 5 27 0.6452760761910667 5 28 0.613067876223031 5 29 0.6540005670600326 5 30 0.6938640886437799 5 31 0.5759235833456309 5 32 0.6044939265501892 5 33 0.6917972847330311 5 34 0.6480061017467502 5 35 0.6073110547567993 5 36 0.6027707534800903 6 1 0.3337888226615675 6 2 0.5738552229831306 6 3 0.5351272256778188 6 4 0.6901768842287446 6 5 0.6258299608776703 6 6 0.5999509898191704 6 7 0.6195310764547806 6 8 0.5869443830211161 6 9 0.6703556702794806 6 10 0.6741426682603979 6 11 0.7481751404489927 6 12 0.6390011305687615 6 13 0.6439070427605316 6 14 0.7051760760488008 6 15 0.8551070457792314 6 16 0.6605472629813001 6 17 0.7675402172418728 6 18 0.7698660011459673 6 19 0.7429065185337613 6 20 0.7718680658526864 6 21 0.7859607517805713 6 22 0.7769829020188812 6 23 0.6947414436440127 6 24 0.7462211528432547 6 25 0.8528027054594158 6 26 0.8276996206250207 6 27 0.8192464627072997 6 28 0.888875440064167 6 29 0.5430146716335305 6 30 0.7145736516363872 6 31 0.6571945071285139 6 32 0.7047036944229852 6 33 0.5905344819442124 6 34 0.6702082997467542 6 35 0.5040878808994478 6 36 0.5552922946237615 7 1 0.5134403926273094 7 2 0.6064008054748022 7 3 0.5856561328710583 7 4 0.6021041894495838 7 5 0.5196249256763927 7 6 0.4753918860949007 7 7 0.4963452697116395 7 8 0.31296402181295235 7 9 0.547711462767623 7 10 0.436975648741214 7 11 0.33712721618575614 7 12 0.34086503214334485 7 13 0.36015685956461496 7 14 0.28897025378932284 7 15 0.44836541107473593 7 16 0.4272115871086868 7 17 0.3258597387622544 7 18 0.3066618246015267 7 19 0.3524975846353726 7 20 0.33439732249189547 7 21 0.36635555237181094 7 22 0.41817030361120366 7 23 0.43600758805958506 7 24 0.3353922447770632 7 25 0.4207199066451661 7 26 0.3472169231793778 7 27 0.36824566656597746 7 28 0.19029884989284973 7 29 0.17103364149698663 7 30 0.16399914472124877 7 31 0.21687676237497477 7 32 0.27210522055577613 7 33 0.2280707627834655 7 34 0.3895477424232574 7 35 0.3055453105158552 7 36 0.25464925688257445 8 1 0.2886043941717098 8 2 0.3342217339140561 8 3 0.40152087378987034 8 4 0.4654412034111921 8 5 0.3365508718164243 8 6 0.2964698032639177 8 7 0.5626287595404307 8 8 0.440593236420753 8 9 0.4437724321119063 8 10 0.5272545789081136 8 11 0.5755979287168884 8 12 0.5098675909215373 8 13 0.5222216116851506 8 14 0.4715991937052273 8 15 0.6324024259735488 8 16 0.618622956011933 8 17 0.5485985564994067 8 18 0.666012449624457 8 19 0.6908483221044008 8 20 0.6818194569790315 8 21 0.6648852508504213 8 22 0.6001517658282172 8 23 0.4851428301523555 8 24 0.6520375924766171 8 25 0.69134337155519 8 26 0.5429174413744882 8 27 0.5661505308717913 8 28 0.5607484578689022 8 29 0.6982050114769365 8 30 0.548013841751116 8 31 0.5299475014960038 8 32 0.5495803130161079 8 33 0.5923501363498875 8 34 0.5444212012737122 8 35 0.27893290619807587 8 36 0.4942160751117033 9 1 0.752932376668273 9 2 0.9426600964641771 9 3 0.8870905097486528 9 4 0.8091115492467615 9 5 0.9473683343787442 9 6 0.9482344508489007 9 7 0.9546505392171873 9 8 0.8610465114743171 9 9 0.8911156569686867 9 10 0.9482919633420615 9 11 0.8733033708880349 9 12 0.9224382084210099 9 13 0.9287068914071988 9 14 0.8159706084743732 9 15 1.0286240745472242 9 16 0.9175969993287474 9 17 0.8138838560278927 9 18 0.9288944438168301 9 19 0.904371153782833 9 20 0.9604441604667926 9 21 0.9617230870229712 9 22 0.693236552182201 9 23 0.8371408560348108 9 24 0.9611101928107431 9 25 0.6484072609059073 9 26 0.9638931497594658 9 27 0.9154346932716138 9 28 0.8565426070753688 9 29 0.7981076860373602 9 30 0.8666541960656531 9 31 0.8960104090860401 9 32 0.8453561764647528 9 33 0.8472292575819598 9 34 0.900084710844574 9 35 0.8919979339607309 9 36 0.9349327490407919 10 1 0.9145879538881765 10 2 0.9719792829740754 10 3 0.9470365940318288 10 4 0.9495484066635207 10 5 0.9174657772853791 10 6 0.9376553677048314 10 7 1.0384271730655286 10 8 0.8210845051451727 10 9 0.893104413892658 10 10 0.940081902616253 10 11 0.8416441520447258 10 12 0.9177770590360552 10 13 0.8536544987581287 10 14 0.8229238278854716 10 15 0.8876837873993608 10 16 0.9704281310099674 10 17 0.9964476985022763 10 18 0.8982561836495258 10 19 0.8725514323991668 10 20 0.9224628567296129 10 21 0.9411625007396047 10 22 0.9872403949710478 10 23 0.8869955621304904 10 24 0.7343305417747352 10 25 0.7956683122943442 10 26 0.7449854244482985 10 27 0.8168807296512579 10 28 0.6468770266138968 10 29 0.7948589468847178 10 30 0.6856390243858677 10 31 0.28884712498166465 10 32 0.13598602008470806 10 33 -1.719816267596487 10 34 0.22542307544451035 10 35 0.27331134552376035 10 36 0.28886901221867056 11 1 0.8135673051689061 11 2 0.8819117428880006 11 3 0.9056481956944005 11 4 0.9621864932170894 11 5 0.8663234810202063 11 6 0.8531028656726818 11 7 1.0595416824273372 11 8 1.0277216355368886 11 9 1.1377115251270666 11 10 0.847562873988128 11 11 0.9885467219583681 11 12 0.9792868027630526 11 13 0.9472080193829626 11 14 1.0058220425266633 11 15 1.0119489689405121 11 16 0.9358797299353583 11 17 0.8259398485104512 11 18 0.9687101750161489 11 19 0.8602615754613012 11 20 0.9950880625736112 11 21 1.02051331582852 11 22 0.9531258687240278 11 23 0.8974401048168872 11 24 1.0392578367039 11 25 0.9235305575573047 11 26 1.0603483612271223 11 27 1.0007147087450026 11 28 1.0008202259636079 11 29 0.9927397081098253 11 30 1.0042184143692676 11 31 0.9420341457158106 11 32 1.0474170818961177 11 33 1.0767642419946064 11 34 1.1331861278944064 11 35 0.8983250027306635 11 36 1.0812125837390738 12 1 0.96950677032968 12 2 1.1296375947363906 12 3 1.130937317573288 12 4 1.2620484814842001 12 5 1.12586614732961 12 6 1.1390215164444086 12 7 1.0601816738083087 12 8 1.1122219414430319 12 9 1.1845709161538451 12 10 1.0952147860074604 12 11 1.1045452553574755 12 12 1.0961857212251758 12 13 1.1117480658705254 12 14 1.0335031677626887 12 15 1.1502139163934615 12 16 1.0611069703076552 12 17 1.061130746062247 12 18 1.1058369786023512 12 19 1.0216523091630307 12 20 1.1380024256473718 12 21 1.0969488152866838 12 22 0.9260112024909386 12 23 0.9327665202043676 12 24 0.8586476271147632 12 25 0.8684244930698828 12 26 1.077549209243129 12 27 1.0303754166293242 12 28 0.9445279188990767 12 29 0.9843374222391041 12 30 0.9181101406295493 12 31 1.0223110580559318 12 32 1.0499379799216761 12 33 0.9123986142409333 12 34 0.9692451647989202 12 35 1.010289602180075 12 36 0.9147428780258586 13 1 1.0088992784425557 13 2 1.219413706105538 13 3 1.0955093834110734 13 4 1.2610865953130137 13 5 1.1091766629883466 13 6 1.1452826318570253 13 7 1.201324584905013 13 8 1.0278302582591543 13 9 1.2574804785116385 13 10 1.1694623855725972 13 11 1.2090555441992414 13 12 1.0234666397579524 13 13 1.1175056741318663 13 14 1.1719607210070784 13 15 1.2391626009851215 13 16 1.2364273455269483 13 17 1.197086898584633 13 18 1.0749276936230139 13 19 1.2038425169175184 13 20 1.2169137777945451 13 21 1.1162538691728976 13 22 1.1066798445098645 13 23 1.2296030438145849 13 24 1.273083233593784 13 25 1.1282769017075776 13 26 1.2364085064919816 13 27 1.2577081160058388 13 28 1.2407277120843148 13 29 1.3029793663331102 13 30 1.178062415411004 13 31 1.2192197990746774 13 32 1.2092641576498266 13 33 1.2770301608541512 13 34 1.272861964932904 13 35 1.2081507007515562 13 36 1.2407036225389998 14 1 1.2180603265960492 14 2 1.3424021967629471 14 3 1.2355102794290893 14 4 1.3571379164896484 14 5 1.255406610358719 14 6 1.1974031612175153 14 7 1.1167390676531603 14 8 1.176585703325049 14 9 1.1008335422552307 14 10 1.1054610703094383 14 11 1.1067573206909955 14 12 1.0824599340549454 14 13 1.1466064274369216 14 14 1.1512636704022121 14 15 1.020845329583409 14 16 1.2375104205696523 14 17 1.0703308858233278 14 18 1.1974769565683658 14 19 1.2478196811557574 14 20 1.1887711802936636 14 21 1.169140736767531 14 22 1.257519164660815 14 23 1.1749110372168516 14 24 1.133420202153026 14 25 1.2405233493094183 14 26 1.146012485046013 14 27 1.1714497768431453 14 28 1.0900198856486796 14 29 1.1397619907625138 14 30 1.2740033706410205 14 31 1.1259845983964212 14 32 1.1191386761326196 14 33 0.924732191255293 14 34 1.1313267122560955 14 35 1.0895074962149813 14 36 1.2423625431721632 15 1 1.013679253126142 15 2 1.234166092464914 15 3 1.1773722536601525 15 4 1.1918325540114694 15 5 1.1834364349542383 15 6 1.1614760642949875 15 7 1.2253741483481804 15 8 1.1318788792351313 15 9 1.2406702516480017 15 10 1.176555250509999 15 11 1.0675113030469667 15 12 1.15560074095767 15 13 1.1183650472474262 15 14 1.0964121995004028 15 15 1.1851924955221769 15 16 1.1731848628583066 15 17 1.0558173686532335 15 18 1.1073442468122927 15 19 1.087610765200477 15 20 1.0854551737501765 15 21 1.1510669621286524 15 22 1.0786991475836114 15 23 1.1848566760461559 15 24 1.114831029572997 15 25 1.2041612967724635 15 26 1.1057991246681116 15 27 1.2267427915030087 15 28 1.105768457199261 15 29 1.1925168711133907 15 30 0.9984312045172956 15 31 1.0443533728984118 15 32 0.9331139981986247 15 33 0.9470902387208984 15 34 1.0469215341151035 15 35 0.7885501564365551 15 36 0.8738133329040085 16 1 1.0126282009629932 16 2 1.1295510215638913 16 3 1.0882293374454548 16 4 1.217553428396208 16 5 1.1136287280052244 16 6 1.2024673282602762 16 7 1.3169639561401567 16 8 1.2748886254381695 16 9 1.3431126013296506 16 10 1.0626641032799995 16 11 1.2897031852185776 16 12 1.1828452008055166 16 13 1.170451817277005 16 14 1.2087970529726384 16 15 1.346763551666065 16 16 1.3855633056208632 16 17 1.2150693004411792 16 18 1.2791950453440641 16 19 1.3383918177262757 16 20 1.2772648173080832 16 21 1.213850578125303 16 22 1.2453384805708705 16 23 1.0008730375676578 16 24 1.045684137056508 16 25 1.0925042917912666 16 26 0.8626593168181191 16 27 1.0750826758591945 16 28 0.9125466663310817 16 29 1.0260707218373248 16 30 1.1282927726253984 16 31 1.1292685419666544 16 32 1.1304430480508358 16 33 0.6968503358349398 16 34 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0.015535780803830335 119 13 -0.05598634881916453 119 14 -0.017550961161003187 119 15 0.06547606340282996 119 16 0.013319945826285817 119 17 0.016346340101112133 119 18 -0.034313352824625554 119 19 -0.02606507976665995 119 20 0.10219070266525065 119 21 -0.0879574542805841 119 22 0.09781173103104202 119 23 -0.09942823747791607 119 24 0.06759711024837323 119 25 -0.034963768968415465 119 26 0.014531807897508742 119 27 0.04092937256889151 119 28 -0.005763345214701232 119 29 -0.06489330146213312 119 30 0.018463111966562806 119 31 0.008663210236269592 119 32 -0.012146677399300643 119 33 0.0049871783293760075 119 34 0.040591054970203544 119 35 0.07206697490011926 119 36 -0.08590123054618282 120 1 -18.66501798704589 120 2 -17.503552997245706 120 3 -17.417017320803335 120 4 -17.118145618061753 120 5 -16.96330774606627 120 6 -16.31595040152594 120 7 -15.952923273363044 120 8 -15.835508901448232 120 9 -15.559290847096353 120 10 -15.433308378977152 120 11 -15.342464707845384 120 12 -15.204776168985923 120 13 -15.079123660582532 120 14 -14.960815869916726 120 15 -14.75725525793031 120 16 -14.673673860748274 120 17 -14.564592823475746 120 18 -14.452877058108715 120 19 -14.308166287381052 120 20 -14.129928111676982 120 21 -14.038555047337319 120 22 -13.869108334424558 120 23 -13.749551484572805 120 24 -13.605663448609882 120 25 -13.46096372754082 120 26 -13.24320997390335 120 27 -13.045198597271819 120 28 -12.94927681953995 120 29 -12.794018972071326 120 30 -12.654569595119627 120 31 -12.577288692326027 120 32 -12.337249728541666 120 33 -12.113682167321763 120 34 -11.891571258346698 120 35 -11.744180927827887 120 36 -11.711002210308802 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 2.1715917E7 3 783536.0 4 928764.0 5 1067149.0 6 765395.0 7 418009.0 8 433892.0 9 466420.0 10 349564.0 11 303301.0 12 316830.0 13 326277.0 14 316042.0 15 307849.0 16 315775.0 17 325863.0 18 333031.0 19 351302.0 20 402065.0 21 471368.0 22 537162.0 23 596778.0 24 657700.0 25 711057.0 26 763677.0 27 819442.0 28 893448.0 29 989414.0 30 1113844.0 31 1271504.0 32 1477419.0 33 1738598.0 34 2047483.0 35 2334136.0 36 2501011.0 37 2338939.0 38 1643678.0 39 633228.0 40 196.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 20.69667306347704 30.44752941138851 23.296982000067683 25.55881552506677 2 25.69660238167212 30.296258984366297 24.1024942967618 19.90464433719978 3 24.718271643696188 28.38013617119418 24.442073917322833 22.459518267786795 4 27.300172524781136 27.328207023147154 23.263042014115058 22.10857843795665 5 27.32947841562164 28.327827028277593 23.83982910079029 20.502865455310477 6 25.551115094024514 27.271609988985684 22.234381460336973 24.94289345665283 7 24.31596210019304 26.804709053528562 25.404810579402483 23.47451826687591 8 24.24511598178187 26.379610415676822 25.15191326716338 24.223360335377933 9 23.53297879464067 26.149714131124842 25.352093900648892 24.9652131735856 10 23.761907177409107 27.47067789418043 23.923176705643844 24.844238222766624 11 24.09177351938834 26.74706184994004 24.421352182072205 24.739812448599416 12 23.98623040944119 26.347589969638907 25.054376654527815 24.611802966392087 13 23.57471519441377 26.64704748459279 25.09154132560952 24.686695995383925 14 23.509860521426326 26.629894738263676 25.279051434695226 24.581193305614775 15 23.212763714775203 26.230682827410213 25.54279703242537 25.01375642538922 16 23.236033837845028 26.032056359964685 25.376131864355006 25.355777937835285 17 23.282506465075553 26.1008826406266 25.36843920716201 25.24817168713584 18 23.141996467656966 26.027823599656386 25.356138055895116 25.47404187679153 19 23.474062754076954 25.952125146836757 25.331157274408078 25.24265482467821 20 23.344180782469596 26.050168178656254 25.340552891515493 25.26509814735866 21 23.221556853986947 26.053111679306724 25.128182611181593 25.597148855524736 22 23.34741054829728 25.946352234494324 25.07166330771896 25.63457390948944 23 23.39750037733675 25.982907917789383 25.14554107151718 25.474050633356686 24 23.325873355491268 25.776142040022037 25.005494254518894 25.8924903499678 25 23.536398305506108 25.761846872379284 24.782323249901385 25.91943157221322 26 23.63288154325929 25.842363977409143 24.96759500096696 25.557159478364603 27 23.51145635801568 25.62437678057697 24.920796645772686 25.94337021563467 28 23.59242179059363 25.78864079621149 24.96609788343926 25.65283952975562 29 23.401117403819143 25.879916034542156 24.972560544896087 25.74640601674262 30 23.395631027903605 25.69037651659219 24.958783538317135 25.955208917187072 31 23.468949499128815 25.709312553161496 24.92624288231347 25.89549506539622 32 23.43324019554059 25.74218688204638 25.009376195350924 25.8151967270621 33 23.314594234739037 25.470694274760763 24.856300340972748 26.358411149527456 34 23.423514651197955 25.557750868594344 24.75554064884458 26.263193831363118 35 23.391092717807993 25.62957722124617 24.883227995495442 26.096102065450395 36 23.277480009157372 25.492361174541635 24.756611167486582 26.47354764881441 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1458740.0 1 1533534.5 2 1608329.0 3 1608329.0 4 1295209.0 5 982089.0 6 982089.0 7 771087.0 8 560085.0 9 560085.0 10 447698.0 11 335311.0 12 335311.0 13 283490.0 14 231669.0 15 227061.5 16 222454.0 17 222454.0 18 261094.5 19 299735.0 20 299735.0 21 381389.5 22 463044.0 23 463044.0 24 580594.5 25 698145.0 26 870993.5 27 1043842.0 28 1043842.0 29 1283584.5 30 1523327.0 31 1523327.0 32 1797655.0 33 2071983.0 34 2071983.0 35 2362635.5 36 2653288.0 37 2653288.0 38 2929341.5 39 3205395.0 40 3475121.5 41 3744848.0 42 3744848.0 43 3974810.5 44 4204773.0 45 4204773.0 46 4344182.0 47 4483591.0 48 4483591.0 49 4503731.5 50 4523872.0 51 4434014.0 52 4344156.0 53 4344156.0 54 4125355.0 55 3906554.0 56 3906554.0 57 3616128.5 58 3325703.0 59 3325703.0 60 2980211.5 61 2634720.0 62 2634720.0 63 2287989.5 64 1941259.0 65 1637742.0 66 1334225.0 67 1334225.0 68 1093164.0 69 852103.0 70 852103.0 71 681963.0 72 511823.0 73 511823.0 74 401551.0 75 291279.0 76 224070.0 77 156861.0 78 156861.0 79 118964.0 80 81067.0 81 81067.0 82 60458.5 83 39850.0 84 39850.0 85 28909.5 86 17969.0 87 17969.0 88 12913.0 89 7857.0 90 5515.5 91 3174.0 92 3174.0 93 2246.0 94 1318.0 95 1318.0 96 1120.5 97 923.0 98 923.0 99 1312.5 100 1702.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 2.28656900972999 2 4.301817638802402 3 4.713296316743207 4 7.4325093784646565 5 7.77590734312566 6 8.581022921039969 7 10.488633161904342 8 8.800028746223315 9 9.870183907943792 10 10.573060313895144 11 10.171152551144555 12 10.97243120755917 13 9.660546271608698 14 9.65674282785355 15 10.646032497627775 16 9.97567228100222 17 9.8575441995037 18 9.960689130444042 19 10.001368309814506 20 10.798179547207182 21 10.878224834412102 22 12.229048107016743 23 10.120562099514343 24 10.217980476262948 25 10.225187490713413 26 10.92631933419908 27 10.248995746708351 28 12.215554716090779 29 10.240962947892466 30 10.971499410336026 31 10.304062916733987 32 12.294532435219681 33 11.025428337047162 34 13.161743649639185 35 10.832827152935618 36 10.895382178490948 >>END_MODULE >>Sequence Length Distribution pass #Length Count 36 5.3767063E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.1745543756426 #Duplication Level Percentage of deduplicated Percentage of total 1 99.96229970609927 99.13716527716825 2 0.027546714537348495 0.054638662775091454 3 0.004678959295136486 0.013921011091107955 4 0.0022580328161922415 0.008957575932457712 5 0.0010045067280371489 0.004981075356020953 6 8.378699143147919E-4 0.004985722522615641 7 0.0 0.0 8 2.518299950928632E-4 0.001998010203340398 9 1.120287660460783E-4 9.999362654885644E-4 >10 9.477542803687058E-4 0.016196989414781895 >50 3.378017218676714E-5 0.0021041233281824446 >100 2.6949437150245214E-5 0.004819662316629907 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 1.8679588329463263E-6 0.7492319536260645 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 404435 0.7521984230382828 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.579624090681688E-6 2.603824575651454E-5 0.0 3.7197493937877915E-6 5.579624090681688E-6 2 9.299373484469478E-6 5.9515990300604664E-5 1.8598746968938958E-6 7.439498787575583E-6 7.439498787575583E-6 3 1.301912287825727E-5 8.183448666333142E-5 1.8598746968938958E-6 1.4878997575151166E-5 9.299373484469478E-6 4 2.603824575651454E-5 1.1903198060120933E-4 1.8598746968938958E-6 1.673887227204506E-5 1.301912287825727E-5 5 3.161786984719623E-5 1.283313540856788E-4 1.8598746968938958E-6 1.673887227204506E-5 1.8598746968938957E-5 6 3.347774454409012E-5 1.4693010105461778E-4 1.8598746968938958E-6 2.0458621665832856E-5 2.0458621665832856E-5 7 4.6496867422347394E-5 1.748282215080262E-4 5.579624090681688E-6 2.7898120453408437E-5 2.4178371059620646E-5 8 5.207649151302908E-5 1.9714671787075295E-4 5.579624090681688E-6 3.905736863477181E-5 2.9757995150302332E-5 9 8.369436136022531E-5 2.213250889303736E-4 5.579624090681688E-6 4.091724333166571E-5 3.161786984719623E-5 10 4.2963105498248995E-4 2.3992383589931256E-4 5.579624090681688E-6 4.46369927254535E-5 3.533761924098402E-5 11 5.300642886147603E-4 2.641022069589332E-4 5.579624090681688E-6 5.0216616816135184E-5 4.46369927254535E-5 12 5.89580278915365E-4 2.845608286247661E-4 5.579624090681688E-6 5.5796240906816874E-5 5.207649151302908E-5 13 6.230580234594551E-4 3.068793249874928E-4 7.439498787575583E-6 5.765611560371077E-5 5.3936366209922975E-5 14 6.472363945190758E-4 3.1989844786575007E-4 7.439498787575583E-6 6.323573969439246E-5 6.137586499749856E-5 15 0.0017222439693237476 3.3105769604711345E-4 7.439498787575583E-6 6.881536378507415E-5 6.509561439128635E-5 16 0.0017910593331088216 3.4407681892537073E-4 7.439498787575583E-6 7.625486257264973E-5 6.695548908818025E-5 17 0.0019063715643162433 3.682551899849914E-4 7.439498787575583E-6 7.997461196643752E-5 7.067523848196804E-5 18 0.002032843043705028 3.998730598321876E-4 9.299373484469478E-6 8.369436136022531E-5 7.253511317886194E-5 19 0.0022746267543012344 4.1661193210423266E-4 9.299373484469478E-6 8.55542360571192E-5 8.55542360571192E-5 20 0.027276922304645877 4.519495513452167E-4 9.299373484469478E-6 8.9273985450907E-5 9.11338601478009E-5 21 0.027795827345079273 4.742680477079434E-4 9.299373484469478E-6 8.9273985450907E-5 9.857335893537648E-5 22 0.027946477195527678 4.8170754649551903E-4 1.1159248181363375E-5 8.9273985450907E-5 1.0415298302605817E-4 23 0.028112006043551234 5.040260428582458E-4 1.1159248181363375E-5 9.671348423848258E-5 1.0973260711673986E-4 24 0.02829055401445305 5.244846645240786E-4 1.1159248181363375E-5 1.0229310832916427E-4 1.1345235651052764E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGATCGG 5280 0.0 9.669837 20 GATCGGA 5300 0.0 9.447668 21 GAGATCG 5770 0.0 8.788615 19 CGGTTCA 6205 0.0 8.704425 1 CGAGATC 5815 0.0 8.692456 18 AATGCCG 7280 0.0 8.068582 13 CCGAGAT 7205 0.0 7.34757 17 GAGCGGT 6795 0.0 7.115159 29 AGCGGTT 6500 0.0 7.0858016 30 ATCGGAA 7310 0.0 7.0420327 22 ATGCCGA 8125 0.0 6.8541307 14 GCCGAGA 8425 0.0 6.824941 16 TGCCGAG 8325 0.0 6.734947 15 TCAACGC 15875 0.0 6.5938144 9 GGTATCA 16445 0.0 6.3268547 5 AGTGGTA 15535 0.0 6.182741 2 ATCAACG 17380 0.0 6.1593857 8 GTATCAA 16780 0.0 6.1445193 6 CAACGCA 16740 0.0 5.9651585 10 TCGGAAG 9220 0.0 5.946854 23 >>END_MODULE